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Protein

Cytochrome b

Gene

Mt-Cyb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.By similarity

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Iron 1 (heme b562 axial ligand)PROSITE-ProRule annotation1
Metal bindingi97Iron 2 (heme b566 axial ligand)By similarity1
Metal bindingi182Iron 1 (heme b562 axial ligand)By similarity1
Metal bindingi196Iron 2 (heme b566 axial ligand)By similarity1
Binding sitei201UbiquinoneBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding, Ubiquinone

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b
Alternative name(s):
Complex III subunit 3
Complex III subunit III
Cytochrome b-c1 complex subunit 3
Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Gene namesi
Name:Mt-Cyb
Synonyms:Cob, Cytb, mt-Cytb, Mtcyb
Encoded oniMitochondrion
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Mitochondrion

Organism-specific databases

MGIiMGI:102501. mt-Cytb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei33 – 53HelicalBy similarityAdd BLAST21
Transmembranei77 – 98HelicalBy similarityAdd BLAST22
Transmembranei113 – 133HelicalBy similarityAdd BLAST21
Transmembranei178 – 198HelicalBy similarityAdd BLAST21
Transmembranei226 – 246HelicalBy similarityAdd BLAST21
Transmembranei288 – 308HelicalBy similarityAdd BLAST21
Transmembranei320 – 340HelicalBy similarityAdd BLAST21
Transmembranei347 – 367HelicalBy similarityAdd BLAST21

GO - Cellular componenti

  • integral component of mitochondrial inner membrane Source: UniProtKB
  • membrane Source: MGI
  • mitochondrion Source: MGI
  • respiratory chain complex III Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000612111 – 381Cytochrome bAdd BLAST381

Proteomic databases

PaxDbiP00158.
PRIDEiP00158.

PTM databases

PhosphoSitePlusiP00158.

Expressioni

Gene expression databases

BgeeiENSMUSG00000064370.
ExpressionAtlasiP00158. baseline and differential.

Interactioni

Subunit structurei

The cytochrome bc1 complex contains 11 subunits: 3 respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). This cytochrome bc1 complex then forms a dimer.By similarity

Protein-protein interaction databases

IntActiP00158. 1 interactor.
STRINGi10090.ENSMUSP00000081003.

Structurei

3D structure databases

ProteinModelPortaliP00158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4663. Eukaryota.
COG1290. LUCA.
GeneTreeiENSGT00390000017948.
HOGENOMiHOG000255206.
HOVERGENiHBG017694.
InParanoidiP00158.
KOiK00412.
OMAiPEHIVPE.
OrthoDBiEOG091G0DW4.
PhylomeDBiP00158.
TreeFamiTF353088.

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
InterProiIPR005798. Cyt_b/b6_C.
IPR005797. Cyt_b/b6_N.
IPR027387. Cytb/b6-like.
IPR030689. Cytochrome_b.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00032. Cytochrom_B_C. 1 hit.
PF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF038885. COB. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
SSF81648. SSF81648. 1 hit.
PROSITEiPS51003. CYTB_CTER. 1 hit.
PS51002. CYTB_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNMRKTHPL FKIINHSFID LPAPSNISSW WNFGSLLGVC LMVQIITGLF
60 70 80 90 100
LAMHYTSDTM TAFSSVTHIC RDVNYGWLIR YMHANGASMF FICLFLHVGR
110 120 130 140 150
GLYYGSYTFM ETWNIGVLLL FAVMATAFMG YVLPWGQMSF WGATVITNLL
160 170 180 190 200
SAIPYIGTTL VEWIWGGFSV DKATLTRFFA FHFILPFIIA ALAIVHLLFL
210 220 230 240 250
HETGSNNPTG LNSDADKIPF HPYYTIKDIL GILIMFLILM TLVLFFPDML
260 270 280 290 300
GDPDNYMPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALILSILI
310 320 330 340 350
LALMPFLHTS KQRSLMFRPI TQILYWILVA NLLILTWIGG QPVEHPFIII
360 370 380
GQLASISYFS IILILMPISG IIEDKMLKLY P
Length:381
Mass (Da):43,210
Last modified:July 21, 1986 - v1
Checksum:i6B46D5B1DD8E001A
GO

Sequence cautioni

The sequence CAA24092 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01420 Genomic DNA. Translation: AAB48656.1.
V00711 Genomic DNA. Translation: CAA24092.1. Different initiation.
AY172335 Genomic DNA. Translation: AAN85134.1.
X57780 mRNA. Translation: CAA40926.1.
PIRiA00153. CBMS.
RefSeqiNP_904340.1. NC_005089.1.

Genome annotation databases

EnsembliENSMUST00000082421; ENSMUSP00000081003; ENSMUSG00000064370.
GeneIDi17711.
KEGGimmu:17711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01420 Genomic DNA. Translation: AAB48656.1.
V00711 Genomic DNA. Translation: CAA24092.1. Different initiation.
AY172335 Genomic DNA. Translation: AAN85134.1.
X57780 mRNA. Translation: CAA40926.1.
PIRiA00153. CBMS.
RefSeqiNP_904340.1. NC_005089.1.

3D structure databases

ProteinModelPortaliP00158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00158. 1 interactor.
STRINGi10090.ENSMUSP00000081003.

PTM databases

PhosphoSitePlusiP00158.

Proteomic databases

PaxDbiP00158.
PRIDEiP00158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082421; ENSMUSP00000081003; ENSMUSG00000064370.
GeneIDi17711.
KEGGimmu:17711.

Organism-specific databases

CTDi4519.
MGIiMGI:102501. mt-Cytb.

Phylogenomic databases

eggNOGiKOG4663. Eukaryota.
COG1290. LUCA.
GeneTreeiENSGT00390000017948.
HOGENOMiHOG000255206.
HOVERGENiHBG017694.
InParanoidiP00158.
KOiK00412.
OMAiPEHIVPE.
OrthoDBiEOG091G0DW4.
PhylomeDBiP00158.
TreeFamiTF353088.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Miscellaneous databases

PROiP00158.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064370.
ExpressionAtlasiP00158. baseline and differential.

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
InterProiIPR005798. Cyt_b/b6_C.
IPR005797. Cyt_b/b6_N.
IPR027387. Cytb/b6-like.
IPR030689. Cytochrome_b.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00032. Cytochrom_B_C. 1 hit.
PF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF038885. COB. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
SSF81648. SSF81648. 1 hit.
PROSITEiPS51003. CYTB_CTER. 1 hit.
PS51002. CYTB_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB_MOUSE
AccessioniPrimary (citable) accession number: P00158
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.By similarity

Caution

The full-length protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.