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P00157

- CYB_BOVIN

UniProt

P00157 - CYB_BOVIN

Protein

Cytochrome b

Gene

MT-CYB

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.

    Cofactori

    Binds 2 heme groups non-covalently.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi83 – 831Iron 1 (heme b562 axial ligand)1 PublicationPROSITE-ProRule annotation
    Metal bindingi97 – 971Iron 2 (heme b566 axial ligand)
    Metal bindingi182 – 1821Iron 1 (heme b562 axial ligand)
    Metal bindingi196 – 1961Iron 2 (heme b566 axial ligand)

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW
    3. oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. respiratory electron transport chain Source: InterPro

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Protein family/group databases

    TCDBi3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b
    Alternative name(s):
    Complex III subunit 3
    Complex III subunit III
    Cytochrome b-c1 complex subunit 3
    Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
    Gene namesi
    Name:MT-CYB
    Synonyms:COB, CYTB, MTCYB
    Encoded oniMitochondrion
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Mitochondrion

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Cytochrome bPRO_0000060686Add
    BLAST

    Proteomic databases

    PRIDEiP00157.

    Interactioni

    Subunit structurei

    The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

    Protein-protein interaction databases

    DIPiDIP-350N.
    IntActiP00157. 2 interactions.

    Structurei

    Secondary structure

    1
    379
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 74
    Helixi9 – 1810
    Beta strandi22 – 243
    Helixi29 – 313
    Helixi33 – 5220
    Turni59 – 613
    Helixi62 – 7211
    Beta strandi73 – 753
    Helixi76 – 10328
    Helixi106 – 1083
    Helixi110 – 13223
    Helixi137 – 14711
    Helixi148 – 1525
    Turni154 – 1563
    Helixi157 – 1659
    Beta strandi167 – 1704
    Helixi172 – 20332
    Helixi214 – 2163
    Beta strandi217 – 2193
    Helixi220 – 24526
    Turni247 – 2504
    Helixi253 – 2564
    Turni261 – 2633
    Helixi272 – 2743
    Helixi275 – 2828
    Beta strandi284 – 2863
    Helixi287 – 29913
    Helixi300 – 3078
    Beta strandi311 – 3155
    Helixi319 – 33921
    Helixi347 – 36317
    Helixi365 – 37612

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BE3X-ray3.00C1-379[»]
    1BGYX-ray3.00C/O1-379[»]
    1L0LX-ray2.35C1-379[»]
    1L0NX-ray2.60C1-379[»]
    1NTKX-ray2.60C1-379[»]
    1NTMX-ray2.40C1-379[»]
    1NTZX-ray2.60C1-379[»]
    1NU1X-ray3.20C1-379[»]
    1PP9X-ray2.10C/P1-379[»]
    1PPJX-ray2.10C/P1-379[»]
    1QCRX-ray2.70C2-379[»]
    1SQBX-ray2.69C1-379[»]
    1SQPX-ray2.70C1-379[»]
    1SQQX-ray3.00C1-379[»]
    1SQVX-ray2.85C1-379[»]
    1SQXX-ray2.60C1-379[»]
    2A06X-ray2.10C/P1-379[»]
    2FYUX-ray2.26C1-379[»]
    2YBBelectron microscopy19.00C/c1-379[»]
    ProteinModelPortaliP00157.
    SMRiP00157. Positions 1-379.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00157.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei33 – 5321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei76 – 9823HelicalSequence AnalysisAdd
    BLAST
    Transmembranei115 – 13521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei140 – 15819HelicalSequence AnalysisAdd
    BLAST
    Transmembranei178 – 19821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei323 – 34321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei346 – 36621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome b family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1290.
    HOGENOMiHOG000255206.
    HOVERGENiHBG017694.
    InParanoidiP00157.
    KOiK00412.
    OMAiNTINQIF.
    OrthoDBiEOG7BGHKX.
    TreeFamiTF353088.

    Family and domain databases

    Gene3Di1.20.810.10. 1 hit.
    InterProiIPR005798. Cyt_b/b6_C.
    IPR005797. Cyt_b/b6_N.
    IPR027387. Cytb/b6-like.
    IPR016174. Di-haem_cyt_TM.
    [Graphical view]
    PfamiPF00032. Cytochrom_B_C. 1 hit.
    PF13631. Cytochrom_B_N_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF81342. SSF81342. 1 hit.
    SSF81648. SSF81648. 1 hit.
    PROSITEiPS51003. CYTB_CTER. 1 hit.
    PS51002. CYTB_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00157-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNIRKSHPL MKIVNNAFID LPAPSNISSW WNFGSLLGIC LILQILTGLF    50
    LAMHYTSDTT TAFSSVTHIC RDVNYGWIIR YMHANGASMF FICLYMHVGR 100
    GLYYGSYTFL ETWNIGVILL LTVMATAFMG YVLPWGQMSF WGATVITNLL 150
    SAIPYIGTNL VEWIWGGFSV DKATLTRFFA FHFILPFIIM AIAMVHLLFL 200
    HETGSNNPTG ISSDVDKIPF HPYYTIKDIL GALLLILALM LLVLFAPDLL 250
    GDPDNYTPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALAFSILI 300
    LALIPLLHTS KQRSMMFRPL SQCLFWALVA DLLTLTWIGG QPVEHPYITI 350
    GQLASVLYFL LILVLMPTAG TIENKLLKW 379
    Length:379
    Mass (Da):42,591
    Last modified:July 21, 1986 - v1
    Checksum:i90160B99E031E016
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti90 – 901F → V in strain: 65, 66 and D.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00654 Genomic DNA. Translation: CAA24007.1.
    AF490528 Genomic DNA. Translation: AAM08329.1.
    AF490529 Genomic DNA. Translation: AAM08342.1.
    AF493541 Genomic DNA. Translation: AAM12801.1.
    AF493542 Genomic DNA. Translation: AAM12814.1.
    PIRiA00152. CBBO.
    RefSeqiYP_209217.1. NC_006853.1.

    Genome annotation databases

    EnsembliENSBTAT00000060567; ENSBTAP00000053148; ENSBTAG00000043550.
    GeneIDi3283889.
    KEGGibta:3283889.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00654 Genomic DNA. Translation: CAA24007.1 .
    AF490528 Genomic DNA. Translation: AAM08329.1 .
    AF490529 Genomic DNA. Translation: AAM08342.1 .
    AF493541 Genomic DNA. Translation: AAM12801.1 .
    AF493542 Genomic DNA. Translation: AAM12814.1 .
    PIRi A00152. CBBO.
    RefSeqi YP_209217.1. NC_006853.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BE3 X-ray 3.00 C 1-379 [» ]
    1BGY X-ray 3.00 C/O 1-379 [» ]
    1L0L X-ray 2.35 C 1-379 [» ]
    1L0N X-ray 2.60 C 1-379 [» ]
    1NTK X-ray 2.60 C 1-379 [» ]
    1NTM X-ray 2.40 C 1-379 [» ]
    1NTZ X-ray 2.60 C 1-379 [» ]
    1NU1 X-ray 3.20 C 1-379 [» ]
    1PP9 X-ray 2.10 C/P 1-379 [» ]
    1PPJ X-ray 2.10 C/P 1-379 [» ]
    1QCR X-ray 2.70 C 2-379 [» ]
    1SQB X-ray 2.69 C 1-379 [» ]
    1SQP X-ray 2.70 C 1-379 [» ]
    1SQQ X-ray 3.00 C 1-379 [» ]
    1SQV X-ray 2.85 C 1-379 [» ]
    1SQX X-ray 2.60 C 1-379 [» ]
    2A06 X-ray 2.10 C/P 1-379 [» ]
    2FYU X-ray 2.26 C 1-379 [» ]
    2YBB electron microscopy 19.00 C/c 1-379 [» ]
    ProteinModelPortali P00157.
    SMRi P00157. Positions 1-379.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-350N.
    IntActi P00157. 2 interactions.

    Protein family/group databases

    TCDBi 3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

    Proteomic databases

    PRIDEi P00157.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000060567 ; ENSBTAP00000053148 ; ENSBTAG00000043550 .
    GeneIDi 3283889.
    KEGGi bta:3283889.

    Organism-specific databases

    CTDi 4519.

    Phylogenomic databases

    eggNOGi COG1290.
    HOGENOMi HOG000255206.
    HOVERGENi HBG017694.
    InParanoidi P00157.
    KOi K00412.
    OMAi NTINQIF.
    OrthoDBi EOG7BGHKX.
    TreeFami TF353088.

    Miscellaneous databases

    EvolutionaryTracei P00157.
    NextBioi 20810196.

    Family and domain databases

    Gene3Di 1.20.810.10. 1 hit.
    InterProi IPR005798. Cyt_b/b6_C.
    IPR005797. Cyt_b/b6_N.
    IPR027387. Cytb/b6-like.
    IPR016174. Di-haem_cyt_TM.
    [Graphical view ]
    Pfami PF00032. Cytochrom_B_C. 1 hit.
    PF13631. Cytochrom_B_N_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81342. SSF81342. 1 hit.
    SSF81648. SSF81648. 1 hit.
    PROSITEi PS51003. CYTB_CTER. 1 hit.
    PS51002. CYTB_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome."
      Anderson S., de Bruijn M.H.L., Coulson A.R., Eperon I.C., Sanger F., Young I.G.
      J. Mol. Biol. 156:683-717(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Heart.
    2. "Evolution of the cytochrome b gene of mammals."
      Irwin D.M., Kocher T.D., Wilson A.C.
      J. Mol. Evol. 32:128-144(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Bos taurus mitochondrial protein coding regions."
      Wettstein P.J.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 65, 66, D and F.
    4. "Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria."
      Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J.
      Science 277:60-66(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
    5. "Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex."
      Iwata S., Lee J.W., Okada K., Lee J.K., Iwata M., Rasmussen B., Link T.A., Ramaswamy S., Jap B.K.
      Science 281:64-71(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    6. "The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition."
      Gao X., Wen X., Yu C., Esser L., Tsao S., Quinn B., Zhang L., Yu L., Xia D.
      Biochemistry 41:11692-11702(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
    7. "Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex."
      Esser L., Quinn B., Li Y.F., Zhang M., Elberry M., Yu L., Yu C.A., Xia D.
      J. Mol. Biol. 341:281-302(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).
    8. "Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern."
      Huang L.S., Cobessi D., Tung E.Y., Berry E.A.
      J. Mol. Biol. 351:573-597(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    9. "Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex."
      Esser L., Gong X., Yang S., Yu L., Yu C.A., Xia D.
      Proc. Natl. Acad. Sci. U.S.A. 103:13045-13050(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS).

    Entry informationi

    Entry nameiCYB_BOVIN
    AccessioniPrimary (citable) accession number: P00157
    Secondary accession number(s): Q8SE06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3