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Protein

Cytochrome b

Gene

MT-CYB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.1 Publication3 Publications

Cofactori

heme6 PublicationsNote: Binds 2 heme groups non-covalently.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Iron 1 (heme b562 axial ligand)PROSITE-ProRule annotationCombined sources6 Publications1
Metal bindingi97Iron 2 (heme b566 axial ligand)Combined sources6 Publications1
Metal bindingi182Iron 1 (heme b562 axial ligand)Combined sources6 Publications1
Metal bindingi196Iron 2 (heme b566 axial ligand)Combined sources6 Publications1
Binding sitei201UbiquinoneCombined sources2 Publications1

GO - Molecular functioni

  • electron carrier activity Source: InterPro
  • heme binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • ubiquinol-cytochrome-c reductase activity Source: UniProtKB
  • ubiquinone binding Source: UniProtKB

GO - Biological processi

  • mitochondrial electron transport, ubiquinol to cytochrome c Source: UniProtKB
  • respiratory electron transport chain Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.

Protein family/group databases

TCDBi3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b
Alternative name(s):
Complex III subunit 3
Complex III subunit III
Cytochrome b-c1 complex subunit 3
Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Gene namesi
Name:MT-CYB
Synonyms:COB, CYTB, MTCYB
Encoded oniMitochondrion
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Mitochondrion

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei33 – 53HelicalCombined sourcesAdd BLAST21
Transmembranei77 – 98HelicalCombined sourcesAdd BLAST22
Transmembranei113 – 133HelicalCombined sourcesAdd BLAST21
Transmembranei178 – 198HelicalCombined sourcesAdd BLAST21
Transmembranei226 – 246HelicalCombined sourcesAdd BLAST21
Transmembranei288 – 308HelicalCombined sourcesAdd BLAST21
Transmembranei320 – 340HelicalCombined sourcesAdd BLAST21
Transmembranei347 – 367HelicalCombined sourcesAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • integral component of mitochondrial membrane Source: UniProtKB
  • intrinsic component of mitochondrial membrane Source: UniProtKB
  • mitochondrial respiratory chain complex III Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000606861 – 379Cytochrome bAdd BLAST379

Proteomic databases

PaxDbiP00157.
PRIDEiP00157.

Expressioni

Tissue specificityi

Detected in heart (at protein level).3 Publications

Gene expression databases

BgeeiENSBTAG00000043550.
ExpressionAtlasiP00157. differential.

Interactioni

Subunit structurei

The cytochrome bc1 complex contains 11 subunits: 3 respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). This cytochrome bc1 complex then forms a dimer.1 Publication6 Publications

Protein-protein interaction databases

DIPiDIP-350N.
IntActiP00157. 2 interactors.
STRINGi9913.ENSBTAP00000053148.

Structurei

Secondary structure

1379
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Helixi9 – 18Combined sources10
Beta strandi22 – 24Combined sources3
Helixi29 – 31Combined sources3
Helixi33 – 52Combined sources20
Turni59 – 61Combined sources3
Helixi62 – 72Combined sources11
Beta strandi73 – 75Combined sources3
Helixi76 – 103Combined sources28
Helixi106 – 108Combined sources3
Helixi110 – 132Combined sources23
Helixi137 – 147Combined sources11
Helixi148 – 152Combined sources5
Turni154 – 156Combined sources3
Helixi157 – 165Combined sources9
Beta strandi167 – 170Combined sources4
Helixi172 – 203Combined sources32
Helixi214 – 216Combined sources3
Beta strandi217 – 219Combined sources3
Helixi220 – 245Combined sources26
Turni247 – 250Combined sources4
Helixi253 – 256Combined sources4
Turni261 – 263Combined sources3
Helixi272 – 274Combined sources3
Helixi275 – 282Combined sources8
Beta strandi284 – 286Combined sources3
Helixi287 – 299Combined sources13
Helixi300 – 307Combined sources8
Beta strandi311 – 315Combined sources5
Helixi319 – 339Combined sources21
Helixi347 – 363Combined sources17
Helixi365 – 376Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BE3X-ray3.00C1-379[»]
1BGYX-ray3.00C/O1-379[»]
1L0LX-ray2.35C1-379[»]
1L0NX-ray2.60C1-379[»]
1NTKX-ray2.60C1-379[»]
1NTMX-ray2.40C1-379[»]
1NTZX-ray2.60C1-379[»]
1NU1X-ray3.20C1-379[»]
1PP9X-ray2.10C/P1-379[»]
1PPJX-ray2.10C/P1-379[»]
1QCRX-ray2.70C2-379[»]
1SQBX-ray2.69C1-379[»]
1SQPX-ray2.70C1-379[»]
1SQQX-ray3.00C1-379[»]
1SQVX-ray2.85C1-379[»]
1SQXX-ray2.60C1-379[»]
2A06X-ray2.10C/P1-379[»]
2FYUX-ray2.26C1-379[»]
2YBBelectron microscopy19.00C/c1-379[»]
4D6TX-ray3.57C/P1-379[»]
4D6UX-ray4.09C/P1-379[»]
5KLVX-ray2.65C1-379[»]
ProteinModelPortaliP00157.
SMRiP00157.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00157.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4663. Eukaryota.
COG1290. LUCA.
GeneTreeiENSGT00390000017948.
HOGENOMiHOG000255206.
HOVERGENiHBG017694.
InParanoidiP00157.
KOiK00412.
OMAiPEHIVPE.
OrthoDBiEOG091G0DW4.
TreeFamiTF353088.

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
InterProiIPR005798. Cyt_b/b6_C.
IPR005797. Cyt_b/b6_N.
IPR027387. Cytb/b6-like.
IPR030689. Cytochrome_b.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00032. Cytochrom_B_C. 1 hit.
PF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF038885. COB. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
SSF81648. SSF81648. 1 hit.
PROSITEiPS51003. CYTB_CTER. 1 hit.
PS51002. CYTB_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNIRKSHPL MKIVNNAFID LPAPSNISSW WNFGSLLGIC LILQILTGLF
60 70 80 90 100
LAMHYTSDTT TAFSSVTHIC RDVNYGWIIR YMHANGASMF FICLYMHVGR
110 120 130 140 150
GLYYGSYTFL ETWNIGVILL LTVMATAFMG YVLPWGQMSF WGATVITNLL
160 170 180 190 200
SAIPYIGTNL VEWIWGGFSV DKATLTRFFA FHFILPFIIM AIAMVHLLFL
210 220 230 240 250
HETGSNNPTG ISSDVDKIPF HPYYTIKDIL GALLLILALM LLVLFAPDLL
260 270 280 290 300
GDPDNYTPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALAFSILI
310 320 330 340 350
LALIPLLHTS KQRSMMFRPL SQCLFWALVA DLLTLTWIGG QPVEHPYITI
360 370
GQLASVLYFL LILVLMPTAG TIENKLLKW
Length:379
Mass (Da):42,591
Last modified:July 21, 1986 - v1
Checksum:i90160B99E031E016
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti90F → V in strain: 65, 66 and D. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA24007.1.
AF490528 Genomic DNA. Translation: AAM08329.1.
AF490529 Genomic DNA. Translation: AAM08342.1.
AF493541 Genomic DNA. Translation: AAM12801.1.
AF493542 Genomic DNA. Translation: AAM12814.1.
PIRiA00152. CBBO.
RefSeqiYP_209217.1. NC_006853.1.

Genome annotation databases

EnsembliENSBTAT00000060567; ENSBTAP00000053148; ENSBTAG00000043550.
GeneIDi3283889.
KEGGibta:3283889.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA24007.1.
AF490528 Genomic DNA. Translation: AAM08329.1.
AF490529 Genomic DNA. Translation: AAM08342.1.
AF493541 Genomic DNA. Translation: AAM12801.1.
AF493542 Genomic DNA. Translation: AAM12814.1.
PIRiA00152. CBBO.
RefSeqiYP_209217.1. NC_006853.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BE3X-ray3.00C1-379[»]
1BGYX-ray3.00C/O1-379[»]
1L0LX-ray2.35C1-379[»]
1L0NX-ray2.60C1-379[»]
1NTKX-ray2.60C1-379[»]
1NTMX-ray2.40C1-379[»]
1NTZX-ray2.60C1-379[»]
1NU1X-ray3.20C1-379[»]
1PP9X-ray2.10C/P1-379[»]
1PPJX-ray2.10C/P1-379[»]
1QCRX-ray2.70C2-379[»]
1SQBX-ray2.69C1-379[»]
1SQPX-ray2.70C1-379[»]
1SQQX-ray3.00C1-379[»]
1SQVX-ray2.85C1-379[»]
1SQXX-ray2.60C1-379[»]
2A06X-ray2.10C/P1-379[»]
2FYUX-ray2.26C1-379[»]
2YBBelectron microscopy19.00C/c1-379[»]
4D6TX-ray3.57C/P1-379[»]
4D6UX-ray4.09C/P1-379[»]
5KLVX-ray2.65C1-379[»]
ProteinModelPortaliP00157.
SMRiP00157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-350N.
IntActiP00157. 2 interactors.
STRINGi9913.ENSBTAP00000053148.

Protein family/group databases

TCDBi3.D.3.2.1. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Proteomic databases

PaxDbiP00157.
PRIDEiP00157.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000060567; ENSBTAP00000053148; ENSBTAG00000043550.
GeneIDi3283889.
KEGGibta:3283889.

Organism-specific databases

CTDi4519.

Phylogenomic databases

eggNOGiKOG4663. Eukaryota.
COG1290. LUCA.
GeneTreeiENSGT00390000017948.
HOGENOMiHOG000255206.
HOVERGENiHBG017694.
InParanoidiP00157.
KOiK00412.
OMAiPEHIVPE.
OrthoDBiEOG091G0DW4.
TreeFamiTF353088.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.

Miscellaneous databases

EvolutionaryTraceiP00157.
PROiP00157.

Gene expression databases

BgeeiENSBTAG00000043550.
ExpressionAtlasiP00157. differential.

Family and domain databases

CDDicd00284. Cytochrome_b_N. 1 hit.
Gene3Di1.20.810.10. 1 hit.
InterProiIPR005798. Cyt_b/b6_C.
IPR005797. Cyt_b/b6_N.
IPR027387. Cytb/b6-like.
IPR030689. Cytochrome_b.
IPR016174. Di-haem_cyt_TM.
[Graphical view]
PfamiPF00032. Cytochrom_B_C. 1 hit.
PF00033. Cytochrome_B. 1 hit.
[Graphical view]
PIRSFiPIRSF038885. COB. 1 hit.
SUPFAMiSSF81342. SSF81342. 1 hit.
SSF81648. SSF81648. 1 hit.
PROSITEiPS51003. CYTB_CTER. 1 hit.
PS51002. CYTB_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB_BOVIN
AccessioniPrimary (citable) accession number: P00157
Secondary accession number(s): Q8SE06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.

Caution

The full-length protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.6 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.