P00133 (CYC3_DESGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cytochrome c3
Organism	Desulfovibrio gigas
Taxonomic identifier	879 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in sulfate respiration coupled with phosphorylation by transferring electrons from the enzyme dehydrogenase to ferredoxin.
Post-translational modification	Binds 4 heme groups per subunit.
Miscellaneous	The second and fourth heme binding sites have unusual CXXXXCH motifs.

Ontologies

Keywords
Biological process	Electron transport Sulfate respiration Transport
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	anaerobic respiration Inferred from electronic annotation. Source: UniProtKB-KW electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 112

112

Cytochrome c3

PRO_0000108360

Sites

Metal binding

26

1

Iron (heme 1 axial ligand)

Metal binding

29

1

Iron (heme 3 axial ligand)

Metal binding

38

1

Iron (heme 1 axial ligand)

Metal binding

39

1

Iron (heme 2 axial ligand)

Metal binding

55

1

Iron (heme 2 axial ligand)

Metal binding

73

1

Iron (heme 4 axial ligand)

Metal binding

87

1

Iron (heme 3 axial ligand)

Metal binding

110

1

Iron (heme 4 axial ligand)

Binding site

34

1

Heme 1 (covalent)

Binding site

37

1

Heme 1 (covalent)

Binding site

49

1

Heme 2 (covalent); atypical

Binding site

54

1

Heme 2 (covalent); atypical

Binding site

83

1

Heme 3 (covalent)

Binding site

86

1

Heme 3 (covalent)

Binding site

104

1

Heme 4 (covalent); atypical

Binding site

109

1

Heme 4 (covalent); atypical

Experimental info

Sequence conflict

21

1

Missing AA sequence Ref.1

Secondary structure

1

.

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112

Helix Strand Turn

Details...

Beta strand

8 – 10

3

Beta strand

22 – 24

3

Helix

27 – 29

3

Helix

34 – 36

3

Turn

43 – 46

4

Beta strand

49 – 55

7

Beta strand

60 – 62

3

Helix

68 – 73

6

Helix

83 – 91

9

Helix

95 – 102

8

Beta strand

104 – 109

6

Sequences

	Sequence		Length	Mass (Da)
	P00133 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 61FF10231F4C0080	FASTA	112	11,978
	10 20 30 40 50 60 VDVPADGAKI DFIAGGEKNL TVVFNHSTHK DVKCDDCHHD PGDKQYAGCT TDGCHNILDK 70 80 90 100 110 ADKSVNSWYK VVHDAKGGAK PTCISCHKDK AGDDKELKKK LTGCKGSACH PS

References

[1]	"The structure of cytochrome c'-3 from Desulfovibrio gigas (NCIB 9332)." Ambler R.P., Bruschi M., le Gall J. FEBS Lett. 5:115-117(1969) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 19364 / DSM 1382 / NCIB 9332 / VKM B-1759.
[2]	Kissinger C. Thesis (1989), University of Seattle, United States Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[3]	"Cytochrome c3 from Desulfovibrio gigas: crystal structure at 1.8-A resolution and evidence for a specific calcium-binding site." Matias P.M., Morais J., Coelho R., Carrondo M.A., Wilson K., Dauter Z., Sieker L. Protein Sci. 5:1342-1354(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), SEQUENCE REVISION TO 21.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

CCDV3G. A00126.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QN0	NMR	-	A	1-112	[»]
1QN1	NMR	-	A	1-112	[»]
1WAD	X-ray	1.80	A	1-112	[»]

ProteinModelPortal

P00133.

SMR

P00133. Positions 1-111.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR002322. Cyt_c_III.
IPR020942. Cyt_c_III_dom.
IPR011031. Multihaem_cyt.
[Graphical view]

Pfam

PF02085. Cytochrom_CIII. 1 hit.
[Graphical view]

PRINTS

PR00609. CYTOCHROMEC3.

PROSITE

PS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P00133.

Entry information

Entry name

CYC3_DESGI

Accession

Primary (citable) accession number: P00133

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program