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P00127

- QCR6_YEAST

UniProt

P00127 - QCR6_YEAST

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Protein

Cytochrome b-c1 complex subunit 6

Gene

QCR6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. QCR6 may mediate formation of the complex between cytochromes c and c1.

GO - Molecular functioni

  1. ubiquinol-cytochrome-c reductase activity Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: SGD
  2. hydrogen ion transmembrane transport Source: GOC
  3. mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

BioCyciYEAST:YFR033C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit 6
Alternative name(s):
Complex III subunit 6
Complex III subunit VI
Cytochrome c1 non-heme 17 kDa protein
Mitochondrial hinge protein
Ubiquinol-cytochrome c reductase complex 17 kDa protein
Gene namesi
Name:QCR6
Synonyms:UCR6
Ordered Locus Names:YFR033C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

CYGDiYFR033c.
SGDiS000001929. QCR6.

Subcellular locationi

Mitochondrion inner membrane 1 Publication

GO - Cellular componenti

  1. mitochondrial intermembrane space Source: SGD
  2. mitochondrial respiratory chain complex III Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Cytochrome b-c1 complex subunit 6PRO_0000193542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi101 ↔ 1236 Publications

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP00127.
PaxDbiP00127.
PeptideAtlasiP00127.

Expressioni

Gene expression databases

GenevestigatoriP00127.

Interactioni

Subunit structurei

Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.

Protein-protein interaction databases

BioGridi31190. 68 interactions.
DIPiDIP-1621N.
IntActiP00127. 4 interactions.
MINTiMINT-404182.
STRINGi4932.YFR033C.

Structurei

Secondary structure

1
147
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi77 – 8610Combined sources
Helixi89 – 11022Combined sources
Helixi114 – 1163Combined sources
Helixi124 – 14219Combined sources
Helixi143 – 1453Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30H74-147[»]
1KB9X-ray2.30F74-147[»]
1KYOX-ray2.97F/Q75-147[»]
1P84X-ray2.50F74-147[»]
2IBZX-ray2.30H74-147[»]
3CX5X-ray1.90F/Q2-147[»]
3CXHX-ray2.50F/Q2-147[»]
4PD4X-ray3.04F74-147[»]
ProteinModelPortaliP00127.
SMRiP00127. Positions 74-147.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00127.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 8055Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the UQCRH/QCR6 family.Curated

Phylogenomic databases

eggNOGiNOG69921.
HOGENOMiHOG000001146.
InParanoidiP00127.
KOiK00416.
OMAiCVERVTR.
OrthoDBiEOG7SBP1B.

Family and domain databases

Gene3Di1.10.287.20. 1 hit.
InterProiIPR003422. Cyt_b-c1_6.
IPR023184. Ubol_cytC_Rdtase_hinge_dom.
[Graphical view]
PANTHERiPTHR15336. PTHR15336. 1 hit.
PfamiPF02320. UCR_hinge. 1 hit.
[Graphical view]
SUPFAMiSSF81531. SSF81531. 1 hit.

Sequencei

Sequence statusi: Complete.

P00127-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGMLELVGEY WEQLKITVVP VVAAAEDDDN EQHEEKAAEG EEKEEENGDE
60 70 80 90 100
DEDEDEDEDD DDDDDEDEEE EEEVTDQLED LREHFKNTEE GKALVHHYEE
110 120 130 140
CAERVKIQQQ QPGYADLEHK EDCVEEFFHL QHYLDTATAP RLFDKLK
Length:147
Mass (Da):17,257
Last modified:November 1, 1995 - v2
Checksum:i1488B9C0EB57BE8F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21G → D in CAA25220. (PubMed:6329732)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00551 Genomic DNA. Translation: CAA25220.1.
D50617 Genomic DNA. Translation: BAA09272.1.
BK006940 Genomic DNA. Translation: DAA12476.1.
PIRiS56288. RDBYUC.
RefSeqiNP_116691.3. NM_001179998.3.

Genome annotation databases

EnsemblFungiiYFR033C; YFR033C; YFR033C.
GeneIDi850593.
KEGGisce:YFR033C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00551 Genomic DNA. Translation: CAA25220.1 .
D50617 Genomic DNA. Translation: BAA09272.1 .
BK006940 Genomic DNA. Translation: DAA12476.1 .
PIRi S56288. RDBYUC.
RefSeqi NP_116691.3. NM_001179998.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EZV X-ray 2.30 H 74-147 [» ]
1KB9 X-ray 2.30 F 74-147 [» ]
1KYO X-ray 2.97 F/Q 75-147 [» ]
1P84 X-ray 2.50 F 74-147 [» ]
2IBZ X-ray 2.30 H 74-147 [» ]
3CX5 X-ray 1.90 F/Q 2-147 [» ]
3CXH X-ray 2.50 F/Q 2-147 [» ]
4PD4 X-ray 3.04 F 74-147 [» ]
ProteinModelPortali P00127.
SMRi P00127. Positions 74-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31190. 68 interactions.
DIPi DIP-1621N.
IntActi P00127. 4 interactions.
MINTi MINT-404182.
STRINGi 4932.YFR033C.

Proteomic databases

MaxQBi P00127.
PaxDbi P00127.
PeptideAtlasi P00127.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR033C ; YFR033C ; YFR033C .
GeneIDi 850593.
KEGGi sce:YFR033C.

Organism-specific databases

CYGDi YFR033c.
SGDi S000001929. QCR6.

Phylogenomic databases

eggNOGi NOG69921.
HOGENOMi HOG000001146.
InParanoidi P00127.
KOi K00416.
OMAi CVERVTR.
OrthoDBi EOG7SBP1B.

Enzyme and pathway databases

BioCyci YEAST:YFR033C-MONOMER.

Miscellaneous databases

EvolutionaryTracei P00127.
NextBioi 966446.
PROi P00127.

Gene expression databases

Genevestigatori P00127.

Family and domain databases

Gene3Di 1.10.287.20. 1 hit.
InterProi IPR003422. Cyt_b-c1_6.
IPR023184. Ubol_cytC_Rdtase_hinge_dom.
[Graphical view ]
PANTHERi PTHR15336. PTHR15336. 1 hit.
Pfami PF02320. UCR_hinge. 1 hit.
[Graphical view ]
SUPFAMi SSF81531. SSF81531. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence of the nuclear gene coding for the 17-kd subunit VI of the yeast ubiquinol-cytochrome c reductase: a protein with an extremely high content of acidic amino acids."
    van Loon A.P.G.M., de Groot R.J., de Haan M., Dekker A., Grivell L.A.
    EMBO J. 3:1039-1043(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
    Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
    Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment."
    Hunte C., Koepke J., Lange C., Rossmanith T., Michel H.
    Structure 8:669-684(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 74-147, DISULFIDE BOND.
  8. "Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure."
    Lange C., Nett J.H., Trumpower B.L., Hunte C.
    EMBO J. 20:6591-6600(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 74-147, DISULFIDE BOND.
  9. "Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c."
    Lange C., Hunte C.
    Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS) OF 75-147, DISULFIDE BOND.
  10. "Structure of the yeast cytochrome bc1 complex with a hydroxyquinone anion Qo site inhibitor bound."
    Palsdottir H., Lojero C.G., Trumpower B.L., Hunte C.
    J. Biol. Chem. 278:31303-31311(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 74-147, DISULFIDE BOND.
  11. "A comparison of stigmatellin conformations, free and bound to the photosynthetic reaction center and the cytochrome bc1 complex."
    Lancaster C.R., Hunte C., Kelley J., Trumpower B.L., Ditchfield R.
    J. Mol. Biol. 368:197-208(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 74-147, DISULFIDE BOND.
  12. "Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer."
    Solmaz S.R., Hunte C.
    J. Biol. Chem. 283:17542-17549(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-147, DISULFIDE BOND.

Entry informationi

Entry nameiQCR6_YEAST
AccessioniPrimary (citable) accession number: P00127
Secondary accession number(s): D6VTR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4490 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3