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P00127

- QCR6_YEAST

UniProt

P00127 - QCR6_YEAST

Protein

Cytochrome b-c1 complex subunit 6

Gene

QCR6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. QCR6 may mediate formation of the complex between cytochromes c and c1.

    GO - Molecular functioni

    1. ubiquinol-cytochrome-c reductase activity Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: SGD
    2. hydrogen ion transmembrane transport Source: GOC
    3. mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Enzyme and pathway databases

    BioCyciYEAST:YFR033C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b-c1 complex subunit 6
    Alternative name(s):
    Complex III subunit 6
    Complex III subunit VI
    Cytochrome c1 non-heme 17 kDa protein
    Mitochondrial hinge protein
    Ubiquinol-cytochrome c reductase complex 17 kDa protein
    Gene namesi
    Name:QCR6
    Synonyms:UCR6
    Ordered Locus Names:YFR033C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFR033c.
    SGDiS000001929. QCR6.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: SGD
    2. mitochondrial respiratory chain complex III Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Cytochrome b-c1 complex subunit 6PRO_0000193542Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi101 ↔ 1236 Publications

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP00127.
    PaxDbiP00127.
    PeptideAtlasiP00127.

    Expressioni

    Gene expression databases

    GenevestigatoriP00127.

    Interactioni

    Subunit structurei

    Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.

    Protein-protein interaction databases

    BioGridi31190. 68 interactions.
    DIPiDIP-1621N.
    IntActiP00127. 4 interactions.
    MINTiMINT-404182.
    STRINGi4932.YFR033C.

    Structurei

    Secondary structure

    1
    147
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi77 – 8610
    Helixi89 – 11022
    Helixi114 – 1163
    Helixi124 – 14219
    Helixi143 – 1453

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EZVX-ray2.30H74-147[»]
    1KB9X-ray2.30F74-147[»]
    1KYOX-ray2.97F/Q75-147[»]
    1P84X-ray2.50F74-147[»]
    2IBZX-ray2.30H74-147[»]
    3CX5X-ray1.90F/Q2-147[»]
    3CXHX-ray2.50F/Q2-147[»]
    4PD4X-ray3.04F74-147[»]
    ProteinModelPortaliP00127.
    SMRiP00127. Positions 74-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00127.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi26 – 8055Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the UQCRH/QCR6 family.Curated

    Phylogenomic databases

    eggNOGiNOG69921.
    HOGENOMiHOG000001146.
    KOiK00416.
    OMAiCVERVTR.
    OrthoDBiEOG7SBP1B.

    Family and domain databases

    Gene3Di1.10.287.20. 1 hit.
    InterProiIPR003422. Cyt_b-c1_6.
    IPR023184. Ubol_cytC_Rdtase_hinge_dom.
    [Graphical view]
    PANTHERiPTHR15336. PTHR15336. 1 hit.
    PfamiPF02320. UCR_hinge. 1 hit.
    [Graphical view]
    SUPFAMiSSF81531. SSF81531. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00127-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGMLELVGEY WEQLKITVVP VVAAAEDDDN EQHEEKAAEG EEKEEENGDE    50
    DEDEDEDEDD DDDDDEDEEE EEEVTDQLED LREHFKNTEE GKALVHHYEE 100
    CAERVKIQQQ QPGYADLEHK EDCVEEFFHL QHYLDTATAP RLFDKLK 147
    Length:147
    Mass (Da):17,257
    Last modified:November 1, 1995 - v2
    Checksum:i1488B9C0EB57BE8F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21G → D in CAA25220. (PubMed:6329732)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00551 Genomic DNA. Translation: CAA25220.1.
    D50617 Genomic DNA. Translation: BAA09272.1.
    BK006940 Genomic DNA. Translation: DAA12476.1.
    PIRiS56288. RDBYUC.
    RefSeqiNP_116691.3. NM_001179998.3.

    Genome annotation databases

    EnsemblFungiiYFR033C; YFR033C; YFR033C.
    GeneIDi850593.
    KEGGisce:YFR033C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00551 Genomic DNA. Translation: CAA25220.1 .
    D50617 Genomic DNA. Translation: BAA09272.1 .
    BK006940 Genomic DNA. Translation: DAA12476.1 .
    PIRi S56288. RDBYUC.
    RefSeqi NP_116691.3. NM_001179998.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EZV X-ray 2.30 H 74-147 [» ]
    1KB9 X-ray 2.30 F 74-147 [» ]
    1KYO X-ray 2.97 F/Q 75-147 [» ]
    1P84 X-ray 2.50 F 74-147 [» ]
    2IBZ X-ray 2.30 H 74-147 [» ]
    3CX5 X-ray 1.90 F/Q 2-147 [» ]
    3CXH X-ray 2.50 F/Q 2-147 [» ]
    4PD4 X-ray 3.04 F 74-147 [» ]
    ProteinModelPortali P00127.
    SMRi P00127. Positions 74-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31190. 68 interactions.
    DIPi DIP-1621N.
    IntActi P00127. 4 interactions.
    MINTi MINT-404182.
    STRINGi 4932.YFR033C.

    Proteomic databases

    MaxQBi P00127.
    PaxDbi P00127.
    PeptideAtlasi P00127.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR033C ; YFR033C ; YFR033C .
    GeneIDi 850593.
    KEGGi sce:YFR033C.

    Organism-specific databases

    CYGDi YFR033c.
    SGDi S000001929. QCR6.

    Phylogenomic databases

    eggNOGi NOG69921.
    HOGENOMi HOG000001146.
    KOi K00416.
    OMAi CVERVTR.
    OrthoDBi EOG7SBP1B.

    Enzyme and pathway databases

    BioCyci YEAST:YFR033C-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P00127.
    NextBioi 966446.
    PROi P00127.

    Gene expression databases

    Genevestigatori P00127.

    Family and domain databases

    Gene3Di 1.10.287.20. 1 hit.
    InterProi IPR003422. Cyt_b-c1_6.
    IPR023184. Ubol_cytC_Rdtase_hinge_dom.
    [Graphical view ]
    PANTHERi PTHR15336. PTHR15336. 1 hit.
    Pfami PF02320. UCR_hinge. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81531. SSF81531. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence of the nuclear gene coding for the 17-kd subunit VI of the yeast ubiquinol-cytochrome c reductase: a protein with an extremely high content of acidic amino acids."
      van Loon A.P.G.M., de Groot R.J., de Haan M., Dekker A., Grivell L.A.
      EMBO J. 3:1039-1043(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae."
      Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.
      Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment."
      Hunte C., Koepke J., Lange C., Rossmanith T., Michel H.
      Structure 8:669-684(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 74-147, DISULFIDE BOND.
    8. "Specific roles of protein-phospholipid interactions in the yeast cytochrome bc1 complex structure."
      Lange C., Nett J.H., Trumpower B.L., Hunte C.
      EMBO J. 20:6591-6600(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 74-147, DISULFIDE BOND.
    9. "Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c."
      Lange C., Hunte C.
      Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS) OF 75-147, DISULFIDE BOND.
    10. "Structure of the yeast cytochrome bc1 complex with a hydroxyquinone anion Qo site inhibitor bound."
      Palsdottir H., Lojero C.G., Trumpower B.L., Hunte C.
      J. Biol. Chem. 278:31303-31311(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 74-147, DISULFIDE BOND.
    11. "A comparison of stigmatellin conformations, free and bound to the photosynthetic reaction center and the cytochrome bc1 complex."
      Lancaster C.R., Hunte C., Kelley J., Trumpower B.L., Ditchfield R.
      J. Mol. Biol. 368:197-208(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 74-147, DISULFIDE BOND.
    12. "Structure of complex III with bound cytochrome c in reduced state and definition of a minimal core interface for electron transfer."
      Solmaz S.R., Hunte C.
      J. Biol. Chem. 283:17542-17549(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-147, DISULFIDE BOND.

    Entry informationi

    Entry nameiQCR6_YEAST
    AccessioniPrimary (citable) accession number: P00127
    Secondary accession number(s): D6VTR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 146 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4490 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3