Cytochrome b-c1 complex subunit 6 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P00127 (QCR6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 133. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome b-c1 complex subunit 6

Alternative name(s):
Complex III subunit 6
Complex III subunit VI
Cytochrome c1 non-heme 17 kDa protein
Mitochondrial hinge protein
Ubiquinol-cytochrome c reductase complex 17 kDa protein

Gene names

Name:	QCR6
Synonyms:	UCR6
Ordered Locus Names:	YFR033C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. QCR6 may mediate formation of the complex between cytochromes c and c1.
Subunit structure	Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 4490 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the UQCRH/QCR6 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from mutant phenotype PubMed 8288589. Source: SGD mitochondrial electron transport, ubiquinol to cytochrome c Inferred from mutant phenotype PubMed 8288589. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial intermembrane space Inferred from direct assay PubMed 22984289. Source: SGD mitochondrial respiratory chain complex III Inferred from direct assay Ref.6. Source: SGD
Molecular_function	ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 147

147

Cytochrome b-c1 complex subunit 6

PRO_0000193542

Regions

Compositional bias

26 – 80

Asp/Glu-rich (acidic)

Experimental info

Sequence conflict

G → D in CAA25220. Ref.1

Secondary structure

147

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P00127 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 1488B9C0EB57BE8F
Show »

FASTA

147

17,257

        10         20         30         40         50         60 
MGMLELVGEY WEQLKITVVP VVAAAEDDDN EQHEEKAAEG EEKEEENGDE DEDEDEDEDD 

        70         80         90        100        110        120 
DDDDDEDEEE EEEVTDQLED LREHFKNTEE GKALVHHYEE CAERVKIQQQ QPGYADLEHK 

       130        140 
EDCVEEFFHL QHYLDTATAP RLFDKLK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of the nuclear gene coding for the 17-kd subunit VI of the yeast ubiquinol-cytochrome c reductase: a protein with an extremely high content of acidic amino acids." van Loon A.P.G.M., de Groot R.J., de Haan M., Dekker A., Grivell L.A. EMBO J. 3:1039-1043(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y. Yeast 12:177-190(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment." Hunte C., Koepke J., Lange C., Rossmanith T., Michel H. Structure 8:669-684(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[7]	"Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c." Lange C., Hunte C. Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X00551 Genomic DNA. Translation: CAA25220.1.
D50617 Genomic DNA. Translation: BAA09272.1.
BK006940 Genomic DNA. Translation: DAA12476.1.

PIR

RDBYUC. S56288.

RefSeq

NP_116691.3. NM_001179998.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EZV	X-ray	2.30	H	74-147	[»]
1KB9	X-ray	2.30	F	74-147	[»]
1KYO	X-ray	2.97	F/Q	75-147	[»]
1P84	X-ray	2.50	F	74-147	[»]
2IBZ	X-ray	2.30	H	74-147	[»]
3CX5	X-ray	1.90	F/Q	2-147	[»]
3CXH	X-ray	2.50	F/Q	2-147	[»]

ProteinModelPortal

P00127.

SMR

P00127. Positions 74-147.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1621N.

IntAct

P00127. 2 interactions.

MINT

MINT-404182.

STRING

4932.YFR033C.

Proteomic databases

PaxDb

P00127.

PeptideAtlas

P00127.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFR033C; YFR033C; YFR033C.

GeneID

850593.

KEGG

sce:YFR033C.
sce:YFR035C.

Organism-specific databases

CYGD

YFR033c.

SGD

S000001929. QCR6.

Phylogenomic databases

eggNOG

NOG69921.

GeneTree

ENSGT00700000105751.

HOGENOM

HOG000001146.

K00416.

OMA

EEFFHLQ.

OrthoDB

EOG4VX5G2.

Gene expression databases

Genevestigator

P00127.

GermOnline

YFR033C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.287.20. 1 hit.

InterPro

IPR003422. Cyt_b-c1_6.
IPR023184. Ubol_cytC_Rdtase_hinge_dom.
[Graphical view]

PANTHER

PTHR15336. PTHR15336. 1 hit.

Pfam

PF02320. UCR_hinge. 1 hit.
[Graphical view]

SUPFAM

SSF81531. Ubol_cytC_Rdtase_hinge_dom. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P00127.

NextBio

966446.

Entry information

Entry name

QCR6_YEAST

Accession

Primary (citable) accession number: P00127
Secondary accession number(s): D6VTR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 133 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents