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P00125

- CY1_BOVIN

UniProt

P00125 - CY1_BOVIN

Protein

Cytochrome c1, heme protein, mitochondrial

Gene

CYC1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211Heme (covalent)
    Binding sitei124 – 1241Heme (covalent)
    Metal bindingi125 – 1251Iron (heme axial ligand)
    Metal bindingi244 – 2441Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c1, heme protein, mitochondrial
    Alternative name(s):
    Complex III subunit 4
    Complex III subunit IV
    Cytochrome b-c1 complex subunit 4
    Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit
    Short name:
    Cytochrome c-1
    Gene namesi
    Name:CYC1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 14

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial inner membrane Source: AgBase
    4. mitochondrion Source: AgBase
    5. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 8484Mitochondrion1 PublicationAdd
    BLAST
    Chaini85 – 325241Cytochrome c1, heme protein, mitochondrialPRO_0000108416Add
    BLAST

    Post-translational modificationi

    Binds 1 heme group per subunit.

    Proteomic databases

    PaxDbiP00125.
    PRIDEiP00125.

    Interactioni

    Subunit structurei

    The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).

    Protein-protein interaction databases

    IntActiP00125. 9 interactions.

    Structurei

    Secondary structure

    1
    325
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi100 – 1023
    Helixi107 – 11913
    Helixi121 – 1233
    Beta strandi127 – 1315
    Helixi132 – 1354
    Turni136 – 1383
    Helixi142 – 1509
    Beta strandi152 – 1565
    Turni160 – 1623
    Beta strandi165 – 1684
    Beta strandi171 – 1744
    Beta strandi178 – 1814
    Helixi182 – 1887
    Turni189 – 1913
    Beta strandi197 – 1993
    Turni200 – 2023
    Beta strandi203 – 2064
    Helixi207 – 2159
    Beta strandi229 – 2335
    Beta strandi235 – 2373
    Beta strandi240 – 2445
    Beta strandi248 – 2503
    Turni256 – 2583
    Helixi263 – 27816
    Helixi282 – 31534
    Beta strandi318 – 3214

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BCCX-ray3.16D85-325[»]
    1BE3X-ray3.00D85-325[»]
    1BGYX-ray3.00D/P85-325[»]
    1L0LX-ray2.35D85-325[»]
    1L0NX-ray2.60D85-325[»]
    1NTKX-ray2.60D85-325[»]
    1NTMX-ray2.40D85-325[»]
    1NTZX-ray2.60D85-325[»]
    1NU1X-ray3.20D85-325[»]
    1PP9X-ray2.10D/Q85-325[»]
    1PPJX-ray2.10D/Q85-325[»]
    1QCRX-ray2.70D251-325[»]
    1SQBX-ray2.69D85-325[»]
    1SQPX-ray2.70D85-325[»]
    1SQQX-ray3.00D85-325[»]
    1SQVX-ray2.85D85-325[»]
    1SQXX-ray2.60D85-325[»]
    2A06X-ray2.10D/Q85-325[»]
    2BCCX-ray3.50D85-325[»]
    2FYUX-ray2.26D85-325[»]
    2YBBelectron microscopy19.00D/d85-325[»]
    3BCCX-ray3.70D85-325[»]
    ProteinModelPortaliP00125.
    SMRiP00125. Positions 85-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00125.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei292 – 30615Helical; Note=Anchors to the membraneSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 209102Cytochrome cPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cytochrome c family.Curated
    Contains 1 cytochrome c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2857.
    GeneTreeiENSGT00390000012445.
    HOGENOMiHOG000003867.
    HOVERGENiHBG001239.
    KOiK00413.
    OMAiGLHPPAY.
    OrthoDBiEOG7GBFX7.
    TreeFamiTF314799.

    Family and domain databases

    Gene3Di1.10.760.10. 1 hit.
    InterProiIPR009056. Cyt_c-like_dom.
    IPR002326. Cyt_c1.
    IPR021157. Cyt_c1_TM_anchor_C.
    [Graphical view]
    PANTHERiPTHR10266. PTHR10266. 1 hit.
    PfamiPF02167. Cytochrom_C1. 1 hit.
    [Graphical view]
    PRINTSiPR00603. CYTOCHROMEC1.
    SUPFAMiSSF46626. SSF46626. 1 hit.
    SSF81496. SSF81496. 1 hit.
    PROSITEiPS51007. CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00125-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAAATLRG AMVGPRGAGL PGARARGLLC GARPGQLPLR TPQAVSLSSK    50
    SGLSRGRKVI LSALGMLAAG GAGLAVALHS AVSASDLELH PPSYPWSHRG 100
    LLSSLDHTSI RRGFQVYKQV CSSCHSMDYV AYRHLVGVCY TEDEAKALAE 150
    EVEVQDGPNE DGEMFMRPGK LSDYFPKPYP NPEAARAANN GALPPDLSYI 200
    VRARHGGEDY VFSLLTGYCE PPTGVSLREG LYFNPYFPGQ AIGMAPPIYN 250
    EVLEFDDGTP ATMSQVAKDV CTFLRWAAEP EHDHRKRMGL KMLLMMGLLL 300
    PLVYAMKRHK WSVLKSRKLA YRPPK 325
    Length:325
    Mass (Da):35,297
    Last modified:June 27, 2006 - v2
    Checksum:i588ED1DEFEAABD6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC109917 mRNA. Translation: AAI09918.1.
    U97172 Genomic DNA. Translation: AAB57834.1.
    PIRiA00118. CCBO1.
    RefSeqiNP_001033179.1. NM_001038090.2.
    UniGeneiBt.1808.

    Genome annotation databases

    EnsembliENSBTAT00000016224; ENSBTAP00000016224; ENSBTAG00000012232.
    GeneIDi512500.
    KEGGibta:512500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC109917 mRNA. Translation: AAI09918.1 .
    U97172 Genomic DNA. Translation: AAB57834.1 .
    PIRi A00118. CCBO1.
    RefSeqi NP_001033179.1. NM_001038090.2.
    UniGenei Bt.1808.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BCC X-ray 3.16 D 85-325 [» ]
    1BE3 X-ray 3.00 D 85-325 [» ]
    1BGY X-ray 3.00 D/P 85-325 [» ]
    1L0L X-ray 2.35 D 85-325 [» ]
    1L0N X-ray 2.60 D 85-325 [» ]
    1NTK X-ray 2.60 D 85-325 [» ]
    1NTM X-ray 2.40 D 85-325 [» ]
    1NTZ X-ray 2.60 D 85-325 [» ]
    1NU1 X-ray 3.20 D 85-325 [» ]
    1PP9 X-ray 2.10 D/Q 85-325 [» ]
    1PPJ X-ray 2.10 D/Q 85-325 [» ]
    1QCR X-ray 2.70 D 251-325 [» ]
    1SQB X-ray 2.69 D 85-325 [» ]
    1SQP X-ray 2.70 D 85-325 [» ]
    1SQQ X-ray 3.00 D 85-325 [» ]
    1SQV X-ray 2.85 D 85-325 [» ]
    1SQX X-ray 2.60 D 85-325 [» ]
    2A06 X-ray 2.10 D/Q 85-325 [» ]
    2BCC X-ray 3.50 D 85-325 [» ]
    2FYU X-ray 2.26 D 85-325 [» ]
    2YBB electron microscopy 19.00 D/d 85-325 [» ]
    3BCC X-ray 3.70 D 85-325 [» ]
    ProteinModelPortali P00125.
    SMRi P00125. Positions 85-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00125. 9 interactions.

    Proteomic databases

    PaxDbi P00125.
    PRIDEi P00125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000016224 ; ENSBTAP00000016224 ; ENSBTAG00000012232 .
    GeneIDi 512500.
    KEGGi bta:512500.

    Organism-specific databases

    CTDi 1537.

    Phylogenomic databases

    eggNOGi COG2857.
    GeneTreei ENSGT00390000012445.
    HOGENOMi HOG000003867.
    HOVERGENi HBG001239.
    KOi K00413.
    OMAi GLHPPAY.
    OrthoDBi EOG7GBFX7.
    TreeFami TF314799.

    Miscellaneous databases

    EvolutionaryTracei P00125.
    NextBioi 20870422.

    Family and domain databases

    Gene3Di 1.10.760.10. 1 hit.
    InterProi IPR009056. Cyt_c-like_dom.
    IPR002326. Cyt_c1.
    IPR021157. Cyt_c1_TM_anchor_C.
    [Graphical view ]
    PANTHERi PTHR10266. PTHR10266. 1 hit.
    Pfami PF02167. Cytochrom_C1. 1 hit.
    [Graphical view ]
    PRINTSi PR00603. CYTOCHROMEC1.
    SUPFAMi SSF46626. SSF46626. 1 hit.
    SSF81496. SSF81496. 1 hit.
    PROSITEi PS51007. CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.
    2. "Structural studies of bovine heart cytochrome c1."
      Wakabayashi S., Matsubara H., Kim C.H., King T.E.
      J. Biol. Chem. 257:9335-9344(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 85-325.
      Tissue: Heart.
    3. "Partial genomic sequence of the bovine cytochrome c1 gene."
      Band M., Ron M.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 120-194.
      Strain: Holstein-Friesian.
    4. "Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria."
      Xia D., Yu C.A., Kim H., Xia J.Z., Kachurin A.M., Zhang L., Yu L., Deisenhofer J.
      Science 277:60-66(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 85-321.
    5. "Complete structure of the 11-subunit bovine mitochondrial cytochrome bc1 complex."
      Iwata S., Lee J.W., Okada K., Lee J.K., Iwata M., Rasmussen B., Link T.A., Ramaswamy S., Jap B.K.
      Science 281:64-71(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 85-321.
    6. "The crystal structure of mitochondrial cytochrome bc1 in complex with famoxadone: the role of aromatic-aromatic interaction in inhibition."
      Gao X., Wen X., Yu C., Esser L., Tsao S., Quinn B., Zhang L., Yu L., Xia D.
      Biochemistry 41:11692-11702(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
    7. "Crystallographic studies of quinol oxidation site inhibitors: a modified classification of inhibitors for the cytochrome bc(1) complex."
      Esser L., Quinn B., Li Y.F., Zhang M., Elberry M., Yu L., Yu C.A., Xia D.
      J. Mol. Biol. 341:281-302(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS).
    8. "Binding of the respiratory chain inhibitor antimycin to the mitochondrial bc1 complex: a new crystal structure reveals an altered intramolecular hydrogen-bonding pattern."
      Huang L.S., Cobessi D., Tung E.Y., Berry E.A.
      J. Mol. Biol. 351:573-597(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    9. "Surface-modulated motion switch: capture and release of iron-sulfur protein in the cytochrome bc1 complex."
      Esser L., Gong X., Yang S., Yu L., Yu C.A., Xia D.
      Proc. Natl. Acad. Sci. U.S.A. 103:13045-13050(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.26 ANGSTROMS).

    Entry informationi

    Entry nameiCY1_BOVIN
    AccessioniPrimary (citable) accession number: P00125
    Secondary accession number(s): Q2TBM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3