Reviewed, UniProtKB/Swiss-Prot P00118 (CYC6_SPIMA)

Names and origin

Protein names

Recommended name:
    Cytochrome c6
Alternative name(s):
    Soluble cytochrome f
    Cytochrome c553
    Cytochrome c-553

Gene names

Name:

petJ

Organism

Spirulina maxima (Arthrospira maxima)

Taxonomic identifier

129910 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriales › Arthrospira

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as an electron carrier between membrane-bound cytochrome b6-f and photosystem I in oxygenic photosynthesis. HAMAP MF_00594
Subunit structure	Monomer. HAMAP MF_00594
Subcellular location	Cellular thylakoid lumen Potential.
Post-translational modification	Binds 1 heme group per subunit. HAMAP MF_00594
Sequence similarities	Belongs to the cytochrome c family. PetJ subfamily.
biophysicochemical properties	Redox potential: E0 is +314 mV. HAMAP MF_00594

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Thylakoid
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 89

89

Cytochrome c6 HAMAP MF_00594

PRO_0000208686

Sites

Metal binding

18

1

Iron (heme axial ligand) HAMAP MF_00594

Metal binding

62

1

Iron (heme axial ligand) HAMAP MF_00594

Binding site

14

1

Heme (covalent) HAMAP MF_00594

Binding site

17

1

Heme (covalent) HAMAP MF_00594

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

89

Helix Strand Turn

Details...

Helix

3 – 13

11

Helix

15 – 18

4

Helix

19 – 21

3

Beta strand

24 – 26

3

Helix

33 – 39

7

Turn

41 – 45

5

Helix

47 – 57

11

Turn

66 – 68

3

Helix

71 – 87

17

Sequences

	Sequence		Length	Mass (Da)
	P00118-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 4796D56B3EA8AF85	FASTA	89	9,236
	10 20 30 40 50 60 GDVAAGASVF SANCAACHMG GRNVIVANKT LSKSDLAKYL KGFDDDAVAA VAYQVTNGKN 70 80 AMPGFNGRLS PKQIEDVAAY VVDQAEKGW

References

[1]	"Amino acid sequence similarity between cytochrome f from a blue-green bacterium and algal chloroplasts." Ambler R.P., Bartsch R.G. Nature 253:285-288(1975) [PubMed: 803642] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Structures of cytochrome c-549 and cytochrome c6 from the cyanobacterium Arthrospira maxima." Sawaya M.R., Krogmann D.W., Serag A., Ho K.K., Yeates T.O., Kerfeld C.A. Biochemistry 40:9215-9225(2001) [PubMed: 11478889] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
[3]	"Structure of cytochrome c6 from Arthrospira maxima: an assembly of 24 subunits in a nearly symmetric shell." Kerfeld C.A., Sawaya M.R., Krogmann D.W., Yeates T.O. Acta Crystallogr. D 58:1104-1110(2002) [PubMed: 12077429] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS).

Cross-references

Sequence databases

PIR

CCSG6. A00110.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F1F	X-ray	2.70	A	1-89	[»]
1KIB	X-ray	3.50	A/B/C/D/E/F/G/H	1-89	[»]

ModBase

Search...

Family and domain databases

HAMAP

MF_00594.
[Tree]

InterPro

IPR003088. Cyt_c_I.
IPR008168. Cyt_C_IC.
IPR009056. Cyt_c_monohaem.
[Graphical view]

Gene3D

G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.

Pfam

PF00034. Cytochrom_C. 1 hit.
[Graphical view]

PRINTS

PR00605. CYTCHROMECIC.

PROSITE

PS51007. CYTC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYC6_SPIMA

Accession

Primary (citable) accession number: P00118

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	March 24, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)