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P00048

- CYC_NEUCR

UniProt

P00048 - CYC_NEUCR

Protein

Cytochrome c

Gene

cyc-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei19 – 191Heme (covalent)
    Binding sitei22 – 221Heme (covalent)
    Metal bindingi23 – 231Iron (heme axial ligand)
    Metal bindingi85 – 851Iron (heme axial ligand)

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. mitochondrial electron transport, cytochrome c to oxygen Source: EnsemblFungi
    2. mitochondrial electron transport, ubiquinol to cytochrome c Source: EnsemblFungi

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c
    Gene namesi
    Name:cyc-1
    Synonyms:cyt-12
    ORF Names:B1K11.020, NCU01808
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 6, Linkage Group II

    Subcellular locationi

    Mitochondrion intermembrane space
    Note: Loosely associated with the inner membrane.

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 108107Cytochrome cPRO_0000108328Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771N6,N6,N6-trimethyllysine1 Publication

    Post-translational modificationi

    Binds 1 heme group per subunit.

    Keywords - PTMi

    Methylation

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU01808.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP00048.
    SMRiP00048. Positions 3-106.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome c family.Curated

    Phylogenomic databases

    eggNOGiCOG3474.
    HOGENOMiHOG000009762.
    KOiK08738.
    OrthoDBiEOG71RXXT.

    Family and domain databases

    Gene3Di1.10.760.10. 1 hit.
    InterProiIPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view]
    PANTHERiPTHR11961. PTHR11961. 1 hit.
    PfamiPF00034. Cytochrom_C. 1 hit.
    [Graphical view]
    PRINTSiPR00604. CYTCHRMECIAB.
    SUPFAMiSSF46626. SSF46626. 1 hit.
    PROSITEiPS51007. CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00048-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFSAGDSKK GANLFKTRCA QCHTLEEGGG NKIGPALHGL FGRKTGSVDG    50
    YAYTDANKQK GITWDENTLF EYLENPKKYI PGTKMAFGGL KKDKDRNDII 100
    TFMKEATA 108
    Length:108
    Mass (Da):11,813
    Last modified:January 23, 2007 - v2
    Checksum:i8150D1A1B92A6E85
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti21 – 211Q → E AA sequence (PubMed:5950080)Curated
    Sequence conflicti24 – 318TLEEGGGN → GEGGNLTQ AA sequence (PubMed:5950080)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti11 – 111G → D in cyt-12-12/cyc-1-1; characterized by slow growth and a deficiency of spectrophotometrically-detectable cytochromes aa3 and c. 2 Publications

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05506 mRNA. Translation: CAA29050.1.
    L19358 Genomic DNA. Translation: AAA92156.1.
    X05507 Genomic DNA. Translation: CAA29051.1.
    AL669998 Genomic DNA. Translation: CAD21169.1.
    CM002237 Genomic DNA. Translation: EAA27250.2.
    PIRiS48221. CCNC.
    RefSeqiXP_956486.2. XM_951393.2.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000001740; EFNCRP00000001740; EFNCRG00000001738.
    GeneIDi3872635.
    KEGGincr:NCU01808.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Life shuttle - Issue 76 of November 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05506 mRNA. Translation: CAA29050.1 .
    L19358 Genomic DNA. Translation: AAA92156.1 .
    X05507 Genomic DNA. Translation: CAA29051.1 .
    AL669998 Genomic DNA. Translation: CAD21169.1 .
    CM002237 Genomic DNA. Translation: EAA27250.2 .
    PIRi S48221. CCNC.
    RefSeqi XP_956486.2. XM_951393.2.

    3D structure databases

    ProteinModelPortali P00048.
    SMRi P00048. Positions 3-106.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU01808.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000001740 ; EFNCRP00000001740 ; EFNCRG00000001738 .
    GeneIDi 3872635.
    KEGGi ncr:NCU01808.

    Phylogenomic databases

    eggNOGi COG3474.
    HOGENOMi HOG000009762.
    KOi K08738.
    OrthoDBi EOG71RXXT.

    Family and domain databases

    Gene3Di 1.10.760.10. 1 hit.
    InterProi IPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view ]
    PANTHERi PTHR11961. PTHR11961. 1 hit.
    Pfami PF00034. Cytochrom_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00604. CYTCHRMECIAB.
    SUPFAMi SSF46626. SSF46626. 1 hit.
    PROSITEi PS51007. CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Deficiency in mRNA splicing in a cytochrome c mutant of Neurospora crassa: importance of carboxy terminus for import of apocytochrome c into mitochondria."
      Stuart R.A., Neupert W., Tropschug M.
      EMBO J. 6:2131-2137(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT ASP-11.
      Strain: 74A.
    2. "Mutations in the structural gene for cytochrome c result in deficiency of both cytochromes aa3 and c in Neurospora crassa."
      Bottorff D.A., Parmaksizoglu S., Lemire E.G., Coffin J.W., Bertrand H., Nargang F.E.
      Curr. Genet. 26:329-335(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASP-11.
    3. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    4. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    5. "Neurospora crassa cytochrome c. II. Chymotryptic peptides, tryptic peptides, cyanogen bromide peptides, and the complete amino acid sequence."
      Heller J., Smith E.L.
      J. Biol. Chem. 241:3165-3180(1966) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-108.
    6. "Neurospora crassa and Humicola lanuginosa cytochromes c: more homology in the heme region."
      Lederer F., Simon A.M.
      Biochem. Biophys. Res. Commun. 56:317-323(1974) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-44.
    7. "Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora."
      Delange R.J., Glazer A.N., Smith E.L.
      J. Biol. Chem. 244:1385-1388(1969) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LYS-77.

    Entry informationi

    Entry nameiCYC_NEUCR
    AccessioniPrimary (citable) accession number: P00048
    Secondary accession number(s): Q7RV53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3