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P00048

- CYC_NEUCR

UniProt

P00048 - CYC_NEUCR

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Protein

Cytochrome c

Gene

cyc-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191Heme (covalent)
Binding sitei22 – 221Heme (covalent)
Metal bindingi23 – 231Iron (heme axial ligand)
Metal bindingi85 – 851Iron (heme axial ligand)

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. mitochondrial electron transport, cytochrome c to oxygen Source: EnsemblFungi
  2. mitochondrial electron transport, ubiquinol to cytochrome c Source: EnsemblFungi
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c
Gene namesi
Name:cyc-1
Synonyms:cyt-12
ORF Names:B1K11.020, NCU01808
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 6, Linkage Group II

Subcellular locationi

Mitochondrion intermembrane space
Note: Loosely associated with the inner membrane.

GO - Cellular componenti

  1. mitochondrial intermembrane space Source: EnsemblFungi
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 108107Cytochrome cPRO_0000108328Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771N6,N6,N6-trimethyllysine1 Publication

Post-translational modificationi

Binds 1 heme group per subunit.

Keywords - PTMi

Methylation

Interactioni

Protein-protein interaction databases

STRINGi5141.NCU01808.1.

Structurei

3D structure databases

ProteinModelPortaliP00048.
SMRiP00048. Positions 3-106.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

eggNOGiCOG3474.
HOGENOMiHOG000009762.
InParanoidiP00048.
KOiK08738.
OrthoDBiEOG71RXXT.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
IPR003088. Cyt_c_dom.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00048-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGFSAGDSKK GANLFKTRCA QCHTLEEGGG NKIGPALHGL FGRKTGSVDG
60 70 80 90 100
YAYTDANKQK GITWDENTLF EYLENPKKYI PGTKMAFGGL KKDKDRNDII

TFMKEATA
Length:108
Mass (Da):11,813
Last modified:January 23, 2007 - v2
Checksum:i8150D1A1B92A6E85
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211Q → E AA sequence (PubMed:5950080)Curated
Sequence conflicti24 – 318TLEEGGGN → GEGGNLTQ AA sequence (PubMed:5950080)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 111G → D in cyt-12-12/cyc-1-1; characterized by slow growth and a deficiency of spectrophotometrically-detectable cytochromes aa3 and c. 2 Publications

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05506 mRNA. Translation: CAA29050.1.
L19358 Genomic DNA. Translation: AAA92156.1.
X05507 Genomic DNA. Translation: CAA29051.1.
AL669998 Genomic DNA. Translation: CAD21169.1.
CM002237 Genomic DNA. Translation: EAA27250.2.
PIRiS48221. CCNC.
RefSeqiXP_956486.2. XM_951393.2.

Genome annotation databases

EnsemblFungiiEFNCRT00000001740; EFNCRP00000001740; EFNCRG00000001738.
GeneIDi3872635.
KEGGincr:NCU01808.

Cross-referencesi

Web resourcesi

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05506 mRNA. Translation: CAA29050.1 .
L19358 Genomic DNA. Translation: AAA92156.1 .
X05507 Genomic DNA. Translation: CAA29051.1 .
AL669998 Genomic DNA. Translation: CAD21169.1 .
CM002237 Genomic DNA. Translation: EAA27250.2 .
PIRi S48221. CCNC.
RefSeqi XP_956486.2. XM_951393.2.

3D structure databases

ProteinModelPortali P00048.
SMRi P00048. Positions 3-106.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU01808.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000001740 ; EFNCRP00000001740 ; EFNCRG00000001738 .
GeneIDi 3872635.
KEGGi ncr:NCU01808.

Phylogenomic databases

eggNOGi COG3474.
HOGENOMi HOG000009762.
InParanoidi P00048.
KOi K08738.
OrthoDBi EOG71RXXT.

Family and domain databases

Gene3Di 1.10.760.10. 1 hit.
InterProi IPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
IPR003088. Cyt_c_dom.
[Graphical view ]
PANTHERi PTHR11961. PTHR11961. 1 hit.
Pfami PF00034. Cytochrom_C. 1 hit.
[Graphical view ]
PRINTSi PR00604. CYTCHRMECIAB.
SUPFAMi SSF46626. SSF46626. 1 hit.
PROSITEi PS51007. CYTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Deficiency in mRNA splicing in a cytochrome c mutant of Neurospora crassa: importance of carboxy terminus for import of apocytochrome c into mitochondria."
    Stuart R.A., Neupert W., Tropschug M.
    EMBO J. 6:2131-2137(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT ASP-11.
    Strain: 74A.
  2. "Mutations in the structural gene for cytochrome c result in deficiency of both cytochromes aa3 and c in Neurospora crassa."
    Bottorff D.A., Parmaksizoglu S., Lemire E.G., Coffin J.W., Bertrand H., Nargang F.E.
    Curr. Genet. 26:329-335(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASP-11.
  3. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  4. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  5. "Neurospora crassa cytochrome c. II. Chymotryptic peptides, tryptic peptides, cyanogen bromide peptides, and the complete amino acid sequence."
    Heller J., Smith E.L.
    J. Biol. Chem. 241:3165-3180(1966) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-108.
  6. "Neurospora crassa and Humicola lanuginosa cytochromes c: more homology in the heme region."
    Lederer F., Simon A.M.
    Biochem. Biophys. Res. Commun. 56:317-323(1974) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-44.
  7. "Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora."
    Delange R.J., Glazer A.N., Smith E.L.
    J. Biol. Chem. 244:1385-1388(1969) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-77.

Entry informationi

Entry nameiCYC_NEUCR
AccessioniPrimary (citable) accession number: P00048
Secondary accession number(s): Q7RV53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3