P00047 (CYC_THELA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cytochrome c
Organism	Thermomyces lanuginosus (Humicola lanuginosa)
Taxonomic identifier	5541 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › mitosporic Ascomycota › Thermomyces

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular location	Mitochondrion intermembrane space. Note: Loosely associated with the inner membrane.
Post-translational modification	Binds 1 heme group per subunit. Lys-94 was found to be 95% trimethylated.
Sequence similarities	Belongs to the cytochrome c family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	Heme Iron Metal-binding
PTM	Methylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 111	111	Cytochrome c		PRO_0000108334
Sites
	Metal binding	26	1	Iron (heme axial ligand)
	Metal binding	88	1	Iron (heme axial ligand)
	Binding site	22	1	Heme (covalent) Ref.1
	Binding site	25	1	Heme (covalent) Ref.1
Amino acid modifications
	Modified residue	80	1	N6,N6-dimethyllysine Ref.1
	Modified residue	94	1	N6,N6,N6-trimethyllysine; partial Ref.1
Experimental info
	Sequence conflict	11 – 12	2	AS → SA AA sequence Ref.1
	Sequence conflict	24	1	Q → E AA sequence Ref.1
	Sequence conflict	27 – 34	8	SVEQGGAN → GEGANVSQ AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00047 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 123501124E3CF317	FASTA	111	12,076
	10 20 30 40 50 60 AKGGSFEPGD ASKGANLFKT RCAQCHSVEQ GGANKIGPNL HGLFGRKTGS VEGYSYTDAN 70 80 90 100 110 KQAGITWNED TLFEYLENPK KFIPGTKMAF GGLKKNKDRN DLITYLKEAT K

References

[1]	"Proteins of the thermophilic fungus Humicola lanuginosa. I. Isolation and amino acid sequence of a cytochrome C." Morgan W.T., Hensley C.P. Jr., Riehm J.P. J. Biol. Chem. 247:6555-6565(1972) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 16455 / CBS 632.91.
[2]	"Neurospora crassa and Humicola lanuginosa cytochromes c: more homology in the heme region." Lederer F., Simon A.M. Biochem. Biophys. Res. Commun. 56:317-323(1974) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-37.

Web resources

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Cross-references

Sequence databases
PIR	CCHL. A92114.
3D structure databases
ProteinModelPortal	P00047.
SMR	P00047. Positions 6-109.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002327. Cyt_c_1A/1B. IPR009056. Cyt_c_dom. IPR003088. Cyt_c_I. [Graphical view]
PANTHER	PTHR11961. PTHR11961. 1 hit.
Pfam	PF00034. Cytochrom_C. 1 hit. [Graphical view]
PRINTS	PR00604. CYTCHRMECIAB.
SUPFAM	SSF46626. Cytochrome_c. 1 hit.
PROSITE	PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYC_THELA

Accession

Primary (citable) accession number: P00047

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program