Reviewed, UniProtKB/Swiss-Prot P00044 (CYC1_YEAST)

Names and origin

Protein names

Recommended name:
    Cytochrome c iso-1

Gene names

Name:	CYC1
Ordered Locus Names:	YJR048W
ORF Names:	J1653

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular location	Mitochondrion intermembrane space.
Developmental stage	Iso-1 is the predominant cytochrome c in aerobically grown cells.
Induction	By oxygen.
Post-translational modification	Binds 1 heme group per subunit. Methylation may enhance its transport into mitochondria.
Miscellaneous	Present with 7330 molecules/cell in log phase SD medium. Ref.11
Sequence similarities	Belongs to the cytochrome c family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	Heme Iron Metal-binding
PTM	Methylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay. Source: SGD mitochondrial electron transport, ubiquinol to cytochrome c Inferred from direct assay. Source: SGD transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial intermembrane space Inferred from direct assay. Source: SGD respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from direct assay. Source: SGD heme binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SFB3	P38810	1	EBI-5393,EBI-17012

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.6

Chain

2 – 109

108

Cytochrome c iso-1

PRO_0000108337

Sites

Metal binding

24

1

Iron (heme axial ligand)

Metal binding

86

1

Iron (heme axial ligand)

Binding site

20

1

Heme (covalent)

Binding site

23

1

Heme (covalent)

Amino acid modifications

Modified residue

78

1

N6,N6,N6-trimethyllysine; by CTM1 Ref.10

Experimental info

Mutagenesis

78

1

K → R: Loss of methylation by CTM1. Ref.10

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

109

Helix Strand Turn

Details...

Helix

9 – 19

11

Turn

20 – 23

4

Helix

56 – 61

6

Helix

67 – 75

9

Helix

77 – 80

4

Helix

94 – 107

14

Sequences

	Sequence		Length	Mass (Da)
	P00044-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 1F8B6CB3B60C0BE8	FASTA	109	12,182
	10 20 30 40 50 60 MTEFKAGSAK KGATLFKTRC LQCHTVEKGG PHKVGPNLHG IFGRHSGQAE GYSYTDANIK 70 80 90 100 KNVLWDENNM SEYLTNPKKY IPGTKMAFGG LKKEKDRNDL ITYLKKACE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae." Smith M., Leung D.W., Gillam S., Astell C.R., Montgomery D.L., Hall B.D. Cell 16:753-761(1979) [PubMed: 222467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of the yeast iso-1-cytochrome c mRNA." Boss J.M., Gillam S., Ziotmer R.S., Smith M. J. Biol. Chem. 256:12958-12961(1981) [PubMed: 6273415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae." Melnick L., Sherman F. Gene 87:157-166(1990) [PubMed: 2158927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 10:811-818(1994) [PubMed: 7975898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The complete amino acid sequence in baker's yeast cytochrome c." Narita K., Titani K. J. Biochem. 65:259-267(1969) [PubMed: 5771953] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-109. Strain: Oviformis.
[7]	"Transcription initiation of the Saccharomyces cerevisiae iso-1-cytochrome c gene. Multiple, independent T-A-T-A sequences." McNeil J.B., Smith M. J. Mol. Biol. 187:363-378(1986) [PubMed: 3009831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
[8]	"Studies on cytochrome c. 8. Synthesis of the protected hexadecapeptide (sequence 93-108) of Baker's yeast iso-1-cytochrome c." Moroder L., Marchiori F., Borin G., Scoffone E. Biopolymers 12:729-750(1973) [PubMed: 4348687] [Abstract] Cited for: SYNTHESIS.
[9]	"Enzymatic methylation of in vitro synthesized apocytochrome c enhances its transport into mitochondria." Park K.S., Frost B., Tuck M., Ho L.L., Kim S., Paik W.K. J. Biol. Chem. 262:14702-14708(1987) [PubMed: 2822698] [Abstract] Cited for: METHYLATION.
[10]	"Cytochrome c methyltransferase, Ctm1p, of yeast." Polevoda B., Martzen M.R., Das B., Phizicky E.M., Sherman F. J. Biol. Chem. 275:20508-20513(2000) [PubMed: 10791961] [Abstract] Cited for: METHYLATION AT LYS-78, MUTAGENESIS OF LYS-78.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Yeast iso-1-cytochrome c. A 2.8-A resolution three-dimensional structure determination." Louie G.V., Hutcheon W.L., Brayer G.D. J. Mol. Biol. 199:295-314(1988) [PubMed: 2832611] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[13]	"High-resolution refinement of yeast iso-1-cytochrome c and comparisons with other eukaryotic cytochromes c." Louie G.V., Brayer G.D. J. Mol. Biol. 214:527-555(1990) [PubMed: 2166169] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.23 ANGSTROMS).
[14]	"Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c." Lange C., Hunte C. Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed: 11880631] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
[15]	"Three-dimensional solution structure of Saccharomyces cerevisiae reduced iso-1-cytochrome c." Baistrocchi P., Banci L., Bertini I., Turano P., Bren K.L., Gray H.B. Biochemistry 35:13788-13796(1996) [PubMed: 8901521] [Abstract] Cited for: STRUCTURE BY NMR.
[16]	"Solution structure of oxidized Saccharomyces cerevisiae iso-1-cytochrome c." Banci L., Bertini I., Bren K.L., Gray H.B., Sompornpisut P., Turano P. Biochemistry 36:8992-9001(1997) [PubMed: 9220987] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Cross-references

Sequence databases

EMBLGenBankDDBJ

M37696 Genomic DNA. Translation: AAB59344.1.
V01298 Genomic DNA. Translation: CAA24605.1.
L26347 Genomic DNA. Translation: AAA62856.1.
L36344 Genomic DNA. Translation: AAA88751.1.
Z49548 Genomic DNA. Translation: CAA89576.1.
X03472 Genomic DNA. Translation: CAA27189.1.

PIR

CCBY. A00037.

RefSeq

NP_012582.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CHH	X-ray	1.97	A	2-107	[»]
1CHI	X-ray	2.00	A	2-107	[»]
1CHJ	X-ray	1.90	A	2-107	[»]
1CIE	X-ray	1.80	A	2-107	[»]
1CIF	X-ray	1.90	A	2-107	[»]
1CIG	X-ray	1.80	A	2-107	[»]
1CIH	X-ray	1.80	A	2-107	[»]
1CRG	X-ray	2.00	A	2-107	[»]
1CRH	X-ray	1.90	A	2-108	[»]
1CRI	X-ray	2.00	A	2-107	[»]
1CRJ	X-ray	2.05	A	2-107	[»]
1CSU	X-ray	1.81	A	2-107	[»]
1CSV	X-ray	1.90	A	2-107	[»]
1CSW	X-ray	1.90	A	2-107	[»]
1CSX	X-ray	1.90	A	2-107	[»]
1CTY	X-ray	2.20	A	2-107	[»]
1CTZ	X-ray	1.90	A	2-107	[»]
1FHB	NMR	-	A	2-107	[»]
1IRV	X-ray	1.90	A	2-107	[»]
1IRW	X-ray	2.00	A	2-107	[»]
1KYO	X-ray	2.97	W	2-109	[»]
1LMS	NMR	-	A	2-107	[»]
1NMI	NMR	-	A	2-107	[»]
1RAP	X-ray	2.25	A	2-108	[»]
1RAQ	X-ray	1.90	A	2-108	[»]
1S6V	X-ray	1.88	B/D	3-107	[»]
1U74	X-ray	2.40	B/D	2-107	[»]
1YCC	X-ray	1.23	A	2-109	[»]
1YFC	NMR	-	A	2-107	[»]
1YIC	NMR	-	A	2-107	[»]
2B0Z	X-ray	2.70	B	2-108	[»]
2B10	X-ray	2.80	B/D	2-108	[»]
2B11	X-ray	2.30	B/D	2-108	[»]
2B12	X-ray	3.02	B	2-108	[»]
2BCN	X-ray	1.70	B	2-107	[»]
2GB8	NMR	-	B	2-108	[»]
2HV4	NMR	-	A	2-107	[»]
2JQR	NMR	-	A	2-107	[»]
2JTI	NMR	-	B	2-109	[»]
2ORL	NMR	-	A	2-107	[»]
2PCC	X-ray	2.30	B/D	2-108	[»]
2YCC	X-ray	1.90	A	2-107	[»]
3CX5	X-ray	1.90	W	2-109	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4265N.

IntAct

P00044. 9 interactions.

STRING

P00044.

Proteomic databases

PeptideAtlas

P00044.

PRIDE

P00044.

Genome annotation databases

Ensembl

YJR048W; YJR048W; YJR048W; Saccharomyces cerevisiae. [Genome view]

GeneID

853507.

GenomeReviews

Gene locus YJR048W in contig Y13136_GR.

KEGG

sce:YJR048W.

NMPDR

fig|4932.3.peg.3556.

Organism-specific databases

CYGD

YJR048w.

SGD

S000003809. CYC1.

Phylogenomic databases

HOGENOM

P00044.

OMA

TMSDYLE.

Gene expression databases

ArrayExpress

P00044.

Genevestigator

P00044.

GermOnline

YJR048W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002327. Cyt_c_1A/1B.
IPR003088. Cyt_c_I.
IPR009056. Cyt_c_monohaem.
[Graphical view]

Gene3D

G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.

PANTHER

PTHR11961. Cyt_CIAB. 1 hit.

Pfam

PF00034. Cytochrom_C. 1 hit.
[Graphical view]

PRINTS

PR00604. CYTCHRMECIAB.

ProDom

PD000375. Cyt_CIAB. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS51007. CYTC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

P00044.

NextBio

974162.

Entry information

Entry name

CYC1_YEAST

Accession

Primary (citable) accession number: P00044

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)