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Protein

Cytochrome c iso-1

Gene

CYC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201Heme (covalent)
Binding sitei23 – 231Heme (covalent)
Metal bindingi24 – 241Iron (heme axial ligand)
Metal bindingi86 – 861Iron (heme axial ligand)

GO - Molecular functioni

  • electron carrier activity Source: SGD
  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • mitochondrial electron transport, cytochrome c to oxygen Source: SGD
  • mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31683-MONOMER.
ReactomeiR-SCE-111457. Release of apoptotic factors from the mitochondria.
R-SCE-111458. Formation of apoptosome.
R-SCE-3299685. Detoxification of Reactive Oxygen Species.

Protein family/group databases

MoonProtiP00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c iso-1
Gene namesi
Name:CYC1
Ordered Locus Names:YJR048W
ORF Names:J1653
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR048W.
SGDiS000003809. CYC1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial intermembrane space Source: SGD
  • respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781K → R: Loss of methylation by CTM1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 109108Cytochrome c iso-1PRO_0000108337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781N6,N6,N6-trimethyllysine; by CTM11 Publication
Modified residuei79 – 791N6,N6,N6-trimethyllysine2 Publications

Post-translational modificationi

Binds 1 heme group per subunit.
Methylation may enhance its transport into mitochondria. Methylation occurs in fungi, plants, and some protozoa, but not in animals. The precise role of methylation at Lys-79 is unknown, but it seems to preclude pro-apoptotic activity, possibly by lowering affinity for APAF1.4 Publications

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP00044.

Expressioni

Developmental stagei

Iso-1 is the predominant cytochrome c in aerobically grown cells.

Inductioni

By oxygen.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CCP1P004313EBI-5393,EBI-4389

Protein-protein interaction databases

BioGridi33801. 78 interactions.
DIPiDIP-4265N.
IntActiP00044. 5 interactions.
MINTiMINT-8285287.

Structurei

Secondary structure

1
109
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 1911Combined sources
Turni20 – 234Combined sources
Beta strandi27 – 293Combined sources
Beta strandi33 – 353Combined sources
Helixi41 – 433Combined sources
Beta strandi44 – 474Combined sources
Beta strandi50 – 523Combined sources
Helixi56 – 616Combined sources
Beta strandi64 – 663Combined sources
Helixi67 – 759Combined sources
Helixi77 – 804Combined sources
Turni82 – 843Combined sources
Beta strandi85 – 884Combined sources
Helixi94 – 10714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CHHX-ray1.97A2-107[»]
1CHIX-ray2.00A2-107[»]
1CHJX-ray1.90A2-107[»]
1CIEX-ray1.80A2-107[»]
1CIFX-ray1.90A2-107[»]
1CIGX-ray1.80A2-107[»]
1CIHX-ray1.80A2-107[»]
1CRGX-ray2.00A2-107[»]
1CRHX-ray1.90A2-109[»]
1CRIX-ray2.00A2-107[»]
1CRJX-ray2.05A2-107[»]
1CSUX-ray1.81A2-107[»]
1CSVX-ray1.90A2-107[»]
1CSWX-ray1.90A2-107[»]
1CSXX-ray1.90A2-107[»]
1CTYX-ray2.20A2-107[»]
1CTZX-ray1.90A2-107[»]
1FHBNMR-A2-107[»]
1IRVX-ray1.90A2-107[»]
1IRWX-ray2.00A2-107[»]
1KYOX-ray2.97W2-109[»]
1LMSNMR-A2-107[»]
1NMINMR-A2-107[»]
1RAPX-ray2.25A2-109[»]
1RAQX-ray1.90A2-109[»]
1S6VX-ray1.88B/D2-107[»]
1U74X-ray2.40B/D2-107[»]
1YCCX-ray1.23A2-109[»]
1YFCNMR-A2-107[»]
1YICNMR-A2-107[»]
2B0ZX-ray2.70B2-109[»]
2B10X-ray2.80B/D2-109[»]
2B11X-ray2.30B/D2-109[»]
2B12X-ray3.02B2-109[»]
2BCNX-ray1.70B2-107[»]
2GB8NMR-B2-109[»]
2HV4NMR-A2-107[»]
2JQRNMR-A2-107[»]
2JTINMR-B2-109[»]
2LIRNMR-A2-107[»]
2LITNMR-A2-107[»]
2MHMNMR-A2-107[»]
2N18NMR-B3-107[»]
C2-109[»]
2ORLNMR-A2-107[»]
2PCCX-ray2.30B/D2-109[»]
2YCCX-ray1.90A2-107[»]
3CX5X-ray1.90W2-109[»]
3TYIX-ray1.40A/B2-107[»]
4MU8X-ray1.45A/B2-107[»]
4N0KX-ray1.05A/B2-107[»]
4P4QX-ray2.01B/D7-109[»]
4Q5PX-ray1.87A/B/C4-107[»]
4QAOX-ray2.10A/B/C4-107[»]
4YE1X-ray1.39A/B2-109[»]
ProteinModelPortaliP00044.
SMRiP00044. Positions 2-109.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00044.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009405.
HOGENOMiHOG000009762.
InParanoidiP00044.
KOiK08738.
OMAiKVGPQLN.
OrthoDBiEOG092C56RQ.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFKAGSAK KGATLFKTRC LQCHTVEKGG PHKVGPNLHG IFGRHSGQAE
60 70 80 90 100
GYSYTDANIK KNVLWDENNM SEYLTNPKKY IPGTKMAFGG LKKEKDRNDL

ITYLKKACE
Length:109
Mass (Da):12,182
Last modified:January 23, 2007 - v2
Checksum:i1F8B6CB3B60C0BE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37696 Genomic DNA. Translation: AAB59344.1.
V01298 Genomic DNA. Translation: CAA24605.1.
L26347 Genomic DNA. Translation: AAA62856.1.
L36344 Genomic DNA. Translation: AAA88751.1.
Z49548 Genomic DNA. Translation: CAA89576.1.
X03472 Genomic DNA. Translation: CAA27189.1.
BK006943 Genomic DNA. Translation: DAA08835.1.
PIRiA00037. CCBY.
RefSeqiNP_012582.1. NM_001181706.1.

Genome annotation databases

EnsemblFungiiYJR048W; YJR048W; YJR048W.
GeneIDi853507.
KEGGisce:YJR048W.

Cross-referencesi

Web resourcesi

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37696 Genomic DNA. Translation: AAB59344.1.
V01298 Genomic DNA. Translation: CAA24605.1.
L26347 Genomic DNA. Translation: AAA62856.1.
L36344 Genomic DNA. Translation: AAA88751.1.
Z49548 Genomic DNA. Translation: CAA89576.1.
X03472 Genomic DNA. Translation: CAA27189.1.
BK006943 Genomic DNA. Translation: DAA08835.1.
PIRiA00037. CCBY.
RefSeqiNP_012582.1. NM_001181706.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CHHX-ray1.97A2-107[»]
1CHIX-ray2.00A2-107[»]
1CHJX-ray1.90A2-107[»]
1CIEX-ray1.80A2-107[»]
1CIFX-ray1.90A2-107[»]
1CIGX-ray1.80A2-107[»]
1CIHX-ray1.80A2-107[»]
1CRGX-ray2.00A2-107[»]
1CRHX-ray1.90A2-109[»]
1CRIX-ray2.00A2-107[»]
1CRJX-ray2.05A2-107[»]
1CSUX-ray1.81A2-107[»]
1CSVX-ray1.90A2-107[»]
1CSWX-ray1.90A2-107[»]
1CSXX-ray1.90A2-107[»]
1CTYX-ray2.20A2-107[»]
1CTZX-ray1.90A2-107[»]
1FHBNMR-A2-107[»]
1IRVX-ray1.90A2-107[»]
1IRWX-ray2.00A2-107[»]
1KYOX-ray2.97W2-109[»]
1LMSNMR-A2-107[»]
1NMINMR-A2-107[»]
1RAPX-ray2.25A2-109[»]
1RAQX-ray1.90A2-109[»]
1S6VX-ray1.88B/D2-107[»]
1U74X-ray2.40B/D2-107[»]
1YCCX-ray1.23A2-109[»]
1YFCNMR-A2-107[»]
1YICNMR-A2-107[»]
2B0ZX-ray2.70B2-109[»]
2B10X-ray2.80B/D2-109[»]
2B11X-ray2.30B/D2-109[»]
2B12X-ray3.02B2-109[»]
2BCNX-ray1.70B2-107[»]
2GB8NMR-B2-109[»]
2HV4NMR-A2-107[»]
2JQRNMR-A2-107[»]
2JTINMR-B2-109[»]
2LIRNMR-A2-107[»]
2LITNMR-A2-107[»]
2MHMNMR-A2-107[»]
2N18NMR-B3-107[»]
C2-109[»]
2ORLNMR-A2-107[»]
2PCCX-ray2.30B/D2-109[»]
2YCCX-ray1.90A2-107[»]
3CX5X-ray1.90W2-109[»]
3TYIX-ray1.40A/B2-107[»]
4MU8X-ray1.45A/B2-107[»]
4N0KX-ray1.05A/B2-107[»]
4P4QX-ray2.01B/D7-109[»]
4Q5PX-ray1.87A/B/C4-107[»]
4QAOX-ray2.10A/B/C4-107[»]
4YE1X-ray1.39A/B2-109[»]
ProteinModelPortaliP00044.
SMRiP00044. Positions 2-109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33801. 78 interactions.
DIPiDIP-4265N.
IntActiP00044. 5 interactions.
MINTiMINT-8285287.

Protein family/group databases

MoonProtiP00044.

Proteomic databases

MaxQBiP00044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR048W; YJR048W; YJR048W.
GeneIDi853507.
KEGGisce:YJR048W.

Organism-specific databases

EuPathDBiFungiDB:YJR048W.
SGDiS000003809. CYC1.

Phylogenomic databases

GeneTreeiENSGT00390000009405.
HOGENOMiHOG000009762.
InParanoidiP00044.
KOiK08738.
OMAiKVGPQLN.
OrthoDBiEOG092C56RQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31683-MONOMER.
ReactomeiR-SCE-111457. Release of apoptotic factors from the mitochondria.
R-SCE-111458. Formation of apoptosome.
R-SCE-3299685. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

EvolutionaryTraceiP00044.
PROiP00044.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYC1_YEAST
AccessioniPrimary (citable) accession number: P00044
Secondary accession number(s): D6VWL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 187 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.