P00026 (CYC_CYPCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c iso-1/iso-2

Gene names

Name:

cyc

Organism

Cyprinus carpio (Common carp)

Taxonomic identifier

7962 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Cyprinus

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular location	Mitochondrion intermembrane space. Note: Loosely associated with the inner membrane.
Post-translational modification	Binds 1 heme group per subunit.
Miscellaneous	The protein was obtained from food carp that consisted of two cross-bred strains, the European carp and the Amur carp. The isocytochromes may be the result of strain differences. The sequence shown is the iso-1 cytochrome c.
Sequence similarities	Belongs to the cytochrome c family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	Heme Iron Metal-binding
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Probable
	Chain	2 – 104	103	Cytochrome c iso-1/iso-2		PRO_0000108251
Sites
	Metal binding	19	1	Iron (heme axial ligand) By similarity
	Metal binding	81	1	Iron (heme axial ligand) By similarity
	Binding site	15	1	Heme (covalent) Ref.1
	Binding site	18	1	Heme (covalent) Ref.1
Amino acid modifications
	Modified residue	2	1	N-acetylglycine Probable Ref.1
Natural variations
	Natural variant	5	1	E → D in isoform iso-2.
Experimental info
	Sequence uncertainty	22	1	E or Q
	Sequence uncertainty	23	1	N or D
	Sequence uncertainty	51	1	D or N
	Sequence uncertainty	53	1	N or D
	Sequence uncertainty	62	1	E or Q
	Sequence uncertainty	70	1	E or Q
	Sequence uncertainty	71	1	N or D

Sequences

	Sequence		Length	Mass (Da)
	P00026 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 23FDB6A70BCD684A	FASTA	104	11,496
	10 20 30 40 50 60 MGDVEKGKKV FVQKCAQCHT VENGGKHKVG PNLWGLFGRK TGQAPGFSYT DANKSKGIVW 70 80 90 100 BEZTLMEYLE NPKKYIPGTK MIFAGIKKKG ERADLIAYLK SATS

References

[1]	"The amino acid sequence of cytochrome c of the carp, Cyprinus carpio." Guertler L., Horstmann H.J. Eur. J. Biochem. 12:48-57(1970) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-104, ACETYLATION AT GLY-2. Strain: Amur carp and European carp.

Web resources

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Cross-references

Sequence databases
PIR	CCCA. A00023.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P00026.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG003023.
Family and domain databases
InterPro	IPR002327. Cyt_c_1A/1B. IPR009056. Cyt_c_dom. IPR003088. Cyt_c_I. [Graphical view]
PANTHER	PTHR11961. PTHR11961. 1 hit.
Pfam	PF00034. Cytochrom_C. 1 hit. [Graphical view]
PRINTS	PR00604. CYTCHRMECIAB.
SUPFAM	SSF46626. Cytochrome_c. 1 hit.
PROSITE	PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYC_CYPCA

Accession

Primary (citable) accession number: P00026

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program