P00024 (CYC_LITCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cytochrome c
Organism	Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)
Taxonomic identifier	8400 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Rana › Aquarana

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Subcellular location	Mitochondrion intermembrane space. Note: Loosely associated with the inner membrane.
Post-translational modification	Binds 1 heme group per subunit.
Sequence similarities	Belongs to the cytochrome c family.
Caution	The positions of the amino acids 89-93 were arranged by homology with other cytochrome c sequences. This order differs from that submitted to PIR by Chan et al. The presence of Lys-105 is not certain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Ligand	Heme Iron Metal-binding
PTM	Acetylation Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Probable
	Chain	2 – 105	104	Cytochrome c		PRO_0000108256
Sites
	Metal binding	19	1	Iron (heme axial ligand) By similarity
	Metal binding	81	1	Iron (heme axial ligand) By similarity
	Binding site	15	1	Heme (covalent) By similarity
	Binding site	18	1	Heme (covalent) By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine Probable
	Disulfide bond	21 ↔ 103
Experimental info
	Sequence uncertainty	13 – 27	15
	Sequence uncertainty	61 – 68	8
	Sequence uncertainty	87 – 93	7
	Sequence uncertainty	99 – 105	7

Sequences

	Sequence		Length	Mass (Da)
	P00024 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5229F869BB3FAF28	FASTA	105	11,590
	10 20 30 40 50 60 MGDVEKGKKI FVQKCAQCHT CEKGGKHKVG PNLYGLIGRK TGQAAGFSYT DANKNKGITW 70 80 90 100 GEDTLMEYLE NPKKYIPGTK MIFAGIKKKG ERQDLIAYLK SACSK

References

[1]	Chan S.K., Walasek O.F., Barlow G.H., Margoliash E. Submitted (JUL-1967) to the PIR data bank Cited for: PROTEIN SEQUENCE OF 2-105.

Web resources

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Cross-references

Sequence databases
PIR	CCFG. A00021.
3D structure databases
ProteinModelPortal	P00024.
SMR	P00024. Positions 2-105.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG003023.
Family and domain databases
InterPro	IPR002327. Cyt_c_1A/1B. IPR009056. Cyt_c_dom. IPR003088. Cyt_c_I. [Graphical view]
PANTHER	PTHR11961. PTHR11961. 1 hit.
Pfam	PF00034. Cytochrom_C. 1 hit. [Graphical view]
PRINTS	PR00604. CYTCHRMECIAB.
SUPFAM	SSF46626. Cytochrome_c. 1 hit.
PROSITE	PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYC_LITCT

Accession

Primary (citable) accession number: P00024

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	March 6, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program