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Protein

Cytochrome c, testis-specific

Gene

Cyct

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15Heme (covalent)PROSITE-ProRule annotation1 Publication1
Binding sitei18Heme (covalent)PROSITE-ProRule annotation1 Publication1
Metal bindingi19Iron (heme axial ligand)1
Metal bindingi81Iron (heme axial ligand)1

GO - Molecular functioni

  • electron transfer activity Source: InterPro
  • heme binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processApoptosis, Electron transport, Respiratory chain, Transport
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c, testis-specific
Gene namesi
Name:Cyct
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88579 Cyct

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001082262 – 105Cytochrome c, testis-specificAdd BLAST104

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine1 Publication1

Post-translational modificationi

Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP00015
PaxDbiP00015
PeptideAtlasiP00015
PRIDEiP00015

PTM databases

iPTMnetiP00015
PhosphoSitePlusiP00015

Expressioni

Tissue specificityi

This is one of two isocytochromes C found in the testis. The other is identical with the form found in other mouse tissues. These cytochromes are assumed to be located in the sperm.

Gene expression databases

BgeeiENSMUSG00000056436
CleanExiMM_CYCT
GenevisibleiP00015 MM

Interactioni

Protein-protein interaction databases

IntActiP00015, 1 interactor
MINTiP00015
STRINGi10090.ENSMUSP00000028430

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 14Combined sources11
Turni15 – 18Combined sources4
Beta strandi39 – 42Combined sources4
Helixi51 – 55Combined sources5
Helixi62 – 70Combined sources9
Helixi72 – 75Combined sources4
Helixi89 – 104Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AIUX-ray1.60A1-105[»]
ProteinModelPortaliP00015
SMRiP00015
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00015

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

eggNOGiKOG3453 Eukaryota
COG3474 LUCA
GeneTreeiENSGT00390000009405
HOGENOMiHOG000009762
HOVERGENiHBG003023
InParanoidiP00015
KOiK08738
OMAiLVWDEAN
OrthoDBiEOG091G0UQY
PhylomeDBiP00015
TreeFamiTF300226

Family and domain databases

Gene3Di1.10.760.10, 1 hit
InterProiView protein in InterPro
IPR009056 Cyt_c-like_dom
IPR036909 Cyt_c-like_dom_sf
IPR002327 Cyt_c_1A/1B
PANTHERiPTHR11961 PTHR11961, 1 hit
PfamiView protein in Pfam
PF00034 Cytochrom_C, 1 hit
PRINTSiPR00604 CYTCHRMECIAB
SUPFAMiSSF46626 SSF46626, 1 hit
PROSITEiView protein in PROSITE
PS51007 CYTC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDAEAGKKI FVQKCAQCHT VEKGGKHKTG PNLWGLFGRK TGQAPGFSYT
60 70 80 90 100
DANKNKGVIW SEETLMEYLE NPKKYIPGTK MIFAGIKKKS EREDLIKYLK

QATSS
Length:105
Mass (Da):11,714
Last modified:January 23, 2007 - v3
Checksum:i00C275FC70B5AAF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20625 mRNA Translation: AAA37501.1
X55771 mRNA Translation: CAA39293.1
AK005582 mRNA Translation: BAB24136.1
AK018833 mRNA Translation: BAB31455.1
AK018851 mRNA Translation: BAB31464.1
CCDSiCCDS16156.1
PIRiB28160 CCMST
RefSeqiNP_034119.1, NM_009989.3
XP_011237575.1, XM_011239273.1
UniGeneiMm.4872

Genome annotation databases

EnsembliENSMUST00000028430; ENSMUSP00000028430; ENSMUSG00000056436
GeneIDi13067
KEGGimmu:13067
UCSCiuc008key.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCYC2_MOUSE
AccessioniPrimary (citable) accession number: P00015
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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