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P00015

- CYC2_MOUSE

UniProt

P00015 - CYC2_MOUSE

Protein

Cytochrome c, testis-specific

Gene

Cyct

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
    Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151Heme (covalent)1 PublicationPROSITE-ProRule annotation
    Binding sitei18 – 181Heme (covalent)1 PublicationPROSITE-ProRule annotation
    Metal bindingi19 – 191Iron (heme axial ligand)
    Metal bindingi81 – 811Iron (heme axial ligand)

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: MGI
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. hydrogen peroxide metabolic process Source: MGI
    3. oxidation-reduction process Source: UniProtKB-KW
    4. positive regulation of intrinsic apoptotic signaling pathway Source: MGI

    Keywords - Biological processi

    Apoptosis, Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c, testis-specific
    Gene namesi
    Name:Cyct
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:88579. Cyct.

    Subcellular locationi

    Mitochondrion intermembrane space
    Note: Loosely associated with the inner membrane.

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 105104Cytochrome c, testis-specificPRO_0000108226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycine1 Publication

    Post-translational modificationi

    Binds 1 heme group per subunit.
    Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP00015.
    PRIDEiP00015.

    PTM databases

    PhosphoSiteiP00015.

    Expressioni

    Tissue specificityi

    This is one of two isocytochromes C found in the testis. The other is identical with the form found in other mouse tissues. These cytochromes are assumed to be located in the sperm.

    Gene expression databases

    BgeeiP00015.
    CleanExiMM_CYCT.
    GenevestigatoriP00015.

    Interactioni

    Protein-protein interaction databases

    IntActiP00015. 1 interaction.
    MINTiMINT-4092706.

    Structurei

    Secondary structure

    1
    105
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1411
    Turni15 – 184
    Beta strandi39 – 424
    Helixi51 – 555
    Helixi62 – 709
    Helixi72 – 754
    Helixi89 – 10416

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AIUX-ray1.60A1-105[»]
    ProteinModelPortaliP00015.
    SMRiP00015. Positions 2-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00015.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome c family.Curated

    Phylogenomic databases

    eggNOGiCOG3474.
    GeneTreeiENSGT00390000009405.
    HOGENOMiHOG000009762.
    HOVERGENiHBG003023.
    InParanoidiP00015.
    KOiK08738.
    OMAiRGGAHKQ.
    OrthoDBiEOG761BWX.
    PhylomeDBiP00015.
    TreeFamiTF300226.

    Family and domain databases

    Gene3Di1.10.760.10. 1 hit.
    InterProiIPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view]
    PANTHERiPTHR11961. PTHR11961. 1 hit.
    PfamiPF00034. Cytochrom_C. 1 hit.
    [Graphical view]
    PRINTSiPR00604. CYTCHRMECIAB.
    SUPFAMiSSF46626. SSF46626. 1 hit.
    PROSITEiPS51007. CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00015-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDAEAGKKI FVQKCAQCHT VEKGGKHKTG PNLWGLFGRK TGQAPGFSYT    50
    DANKNKGVIW SEETLMEYLE NPKKYIPGTK MIFAGIKKKS EREDLIKYLK 100
    QATSS 105
    Length:105
    Mass (Da):11,714
    Last modified:January 23, 2007 - v3
    Checksum:i00C275FC70B5AAF9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20625 mRNA. Translation: AAA37501.1.
    X55771 mRNA. Translation: CAA39293.1.
    AK005582 mRNA. Translation: BAB24136.1.
    AK018833 mRNA. Translation: BAB31455.1.
    AK018851 mRNA. Translation: BAB31464.1.
    CCDSiCCDS16156.1.
    PIRiB28160. CCMST.
    RefSeqiNP_034119.1. NM_009989.2.
    XP_006498733.1. XM_006498670.1.
    UniGeneiMm.4872.

    Genome annotation databases

    EnsembliENSMUST00000028430; ENSMUSP00000028430; ENSMUSG00000056436.
    GeneIDi13067.
    KEGGimmu:13067.
    UCSCiuc008key.1. mouse.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Life shuttle - Issue 76 of November 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20625 mRNA. Translation: AAA37501.1 .
    X55771 mRNA. Translation: CAA39293.1 .
    AK005582 mRNA. Translation: BAB24136.1 .
    AK018833 mRNA. Translation: BAB31455.1 .
    AK018851 mRNA. Translation: BAB31464.1 .
    CCDSi CCDS16156.1.
    PIRi B28160. CCMST.
    RefSeqi NP_034119.1. NM_009989.2.
    XP_006498733.1. XM_006498670.1.
    UniGenei Mm.4872.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AIU X-ray 1.60 A 1-105 [» ]
    ProteinModelPortali P00015.
    SMRi P00015. Positions 2-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00015. 1 interaction.
    MINTi MINT-4092706.

    PTM databases

    PhosphoSitei P00015.

    Proteomic databases

    PaxDbi P00015.
    PRIDEi P00015.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028430 ; ENSMUSP00000028430 ; ENSMUSG00000056436 .
    GeneIDi 13067.
    KEGGi mmu:13067.
    UCSCi uc008key.1. mouse.

    Organism-specific databases

    CTDi 13067.
    MGIi MGI:88579. Cyct.

    Phylogenomic databases

    eggNOGi COG3474.
    GeneTreei ENSGT00390000009405.
    HOGENOMi HOG000009762.
    HOVERGENi HBG003023.
    InParanoidi P00015.
    KOi K08738.
    OMAi RGGAHKQ.
    OrthoDBi EOG761BWX.
    PhylomeDBi P00015.
    TreeFami TF300226.

    Miscellaneous databases

    EvolutionaryTracei P00015.
    NextBioi 283000.
    PROi P00015.
    SOURCEi Search...

    Gene expression databases

    Bgeei P00015.
    CleanExi MM_CYCT.
    Genevestigatori P00015.

    Family and domain databases

    Gene3Di 1.10.760.10. 1 hit.
    InterProi IPR009056. Cyt_c-like_dom.
    IPR002327. Cyt_c_1A/1B.
    IPR003088. Cyt_c_dom.
    [Graphical view ]
    PANTHERi PTHR11961. PTHR11961. 1 hit.
    Pfami PF00034. Cytochrom_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00604. CYTCHRMECIAB.
    SUPFAMi SSF46626. SSF46626. 1 hit.
    PROSITEi PS51007. CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of rodent genes encoding the testis-specific cytochrome c. Differences in gene structure and evolution between somatic and testicular variants."
      Virbasius J.V., Scarpulla R.C.
      J. Biol. Chem. 263:6791-6796(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "Change of cytochrome c structure during development of the mouse."
      Hennig B.
      Eur. J. Biochem. 55:167-183(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-105, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT GLY-2.
      Strain: BALB/c.

    Entry informationi

    Entry nameiCYC2_MOUSE
    AccessioniPrimary (citable) accession number: P00015
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 133 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3