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P00015

- CYC2_MOUSE

UniProt

P00015 - CYC2_MOUSE

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Protein

Cytochrome c, testis-specific

Gene

Cyct

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151Heme (covalent)1 PublicationPROSITE-ProRule annotation
Binding sitei18 – 181Heme (covalent)1 PublicationPROSITE-ProRule annotation
Metal bindingi19 – 191Iron (heme axial ligand)
Metal bindingi81 – 811Iron (heme axial ligand)

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. heme binding Source: MGI
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. hydrogen peroxide metabolic process Source: MGI
  3. oxidation-reduction process Source: UniProtKB-KW
  4. positive regulation of intrinsic apoptotic signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c, testis-specific
Gene namesi
Name:Cyct
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:88579. Cyct.

Subcellular locationi

Mitochondrion intermembrane space
Note: Loosely associated with the inner membrane.

GO - Cellular componenti

  1. mitochondrion Source: MGI
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 105104Cytochrome c, testis-specificPRO_0000108226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication

Post-translational modificationi

Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00015.
PRIDEiP00015.

PTM databases

PhosphoSiteiP00015.

Expressioni

Tissue specificityi

This is one of two isocytochromes C found in the testis. The other is identical with the form found in other mouse tissues. These cytochromes are assumed to be located in the sperm.

Gene expression databases

BgeeiP00015.
CleanExiMM_CYCT.
ExpressionAtlasiP00015. baseline and differential.
GenevestigatoriP00015.

Interactioni

Protein-protein interaction databases

IntActiP00015. 1 interaction.
MINTiMINT-4092706.

Structurei

Secondary structure

1
105
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1411Combined sources
Turni15 – 184Combined sources
Beta strandi39 – 424Combined sources
Helixi51 – 555Combined sources
Helixi62 – 709Combined sources
Helixi72 – 754Combined sources
Helixi89 – 10416Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AIUX-ray1.60A1-105[»]
ProteinModelPortaliP00015.
SMRiP00015. Positions 2-105.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00015.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

eggNOGiCOG3474.
GeneTreeiENSGT00390000009405.
HOGENOMiHOG000009762.
HOVERGENiHBG003023.
InParanoidiP00015.
KOiK08738.
OMAiRGGAHKQ.
OrthoDBiEOG761BWX.
PhylomeDBiP00015.
TreeFamiTF300226.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
IPR003088. Cyt_c_dom.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00015-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDAEAGKKI FVQKCAQCHT VEKGGKHKTG PNLWGLFGRK TGQAPGFSYT
60 70 80 90 100
DANKNKGVIW SEETLMEYLE NPKKYIPGTK MIFAGIKKKS EREDLIKYLK

QATSS
Length:105
Mass (Da):11,714
Last modified:January 23, 2007 - v3
Checksum:i00C275FC70B5AAF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20625 mRNA. Translation: AAA37501.1.
X55771 mRNA. Translation: CAA39293.1.
AK005582 mRNA. Translation: BAB24136.1.
AK018833 mRNA. Translation: BAB31455.1.
AK018851 mRNA. Translation: BAB31464.1.
CCDSiCCDS16156.1.
PIRiB28160. CCMST.
RefSeqiNP_034119.1. NM_009989.3.
XP_006498733.1. XM_006498670.1.
UniGeneiMm.4872.

Genome annotation databases

EnsembliENSMUST00000028430; ENSMUSP00000028430; ENSMUSG00000056436.
GeneIDi13067.
KEGGimmu:13067.
UCSCiuc008key.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20625 mRNA. Translation: AAA37501.1 .
X55771 mRNA. Translation: CAA39293.1 .
AK005582 mRNA. Translation: BAB24136.1 .
AK018833 mRNA. Translation: BAB31455.1 .
AK018851 mRNA. Translation: BAB31464.1 .
CCDSi CCDS16156.1.
PIRi B28160. CCMST.
RefSeqi NP_034119.1. NM_009989.3.
XP_006498733.1. XM_006498670.1.
UniGenei Mm.4872.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AIU X-ray 1.60 A 1-105 [» ]
ProteinModelPortali P00015.
SMRi P00015. Positions 2-105.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P00015. 1 interaction.
MINTi MINT-4092706.

PTM databases

PhosphoSitei P00015.

Proteomic databases

PaxDbi P00015.
PRIDEi P00015.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028430 ; ENSMUSP00000028430 ; ENSMUSG00000056436 .
GeneIDi 13067.
KEGGi mmu:13067.
UCSCi uc008key.1. mouse.

Organism-specific databases

CTDi 13067.
MGIi MGI:88579. Cyct.

Phylogenomic databases

eggNOGi COG3474.
GeneTreei ENSGT00390000009405.
HOGENOMi HOG000009762.
HOVERGENi HBG003023.
InParanoidi P00015.
KOi K08738.
OMAi RGGAHKQ.
OrthoDBi EOG761BWX.
PhylomeDBi P00015.
TreeFami TF300226.

Miscellaneous databases

ChiTaRSi Cyct. mouse.
EvolutionaryTracei P00015.
NextBioi 283000.
PROi P00015.
SOURCEi Search...

Gene expression databases

Bgeei P00015.
CleanExi MM_CYCT.
ExpressionAtlasi P00015. baseline and differential.
Genevestigatori P00015.

Family and domain databases

Gene3Di 1.10.760.10. 1 hit.
InterProi IPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
IPR003088. Cyt_c_dom.
[Graphical view ]
PANTHERi PTHR11961. PTHR11961. 1 hit.
Pfami PF00034. Cytochrom_C. 1 hit.
[Graphical view ]
PRINTSi PR00604. CYTCHRMECIAB.
SUPFAMi SSF46626. SSF46626. 1 hit.
PROSITEi PS51007. CYTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of rodent genes encoding the testis-specific cytochrome c. Differences in gene structure and evolution between somatic and testicular variants."
    Virbasius J.V., Scarpulla R.C.
    J. Biol. Chem. 263:6791-6796(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "Change of cytochrome c structure during development of the mouse."
    Hennig B.
    Eur. J. Biochem. 55:167-183(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-105, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT GLY-2.
    Strain: BALB/c.

Entry informationi

Entry nameiCYC2_MOUSE
AccessioniPrimary (citable) accession number: P00015
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3