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Protein

Cytochrome c

Gene

CYCS

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151Heme (covalent)
Binding sitei18 – 181Heme (covalent)
Metal bindingi19 – 191Iron (heme axial ligand)
Metal bindingi81 – 811Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_275643. Formation of apoptosome.
REACT_284445. Respiratory electron transport.
REACT_288409. Transcriptional activation of mitochondrial biogenesis.
REACT_310683. Detoxification of Reactive Oxygen Species.
REACT_332058. Activation of caspases through apoptosome-mediated cleavage.
REACT_346812. Release of apoptotic factors from the mitochondria.
REACT_359221. TP53 Regulates Metabolic Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c
Gene namesi
Name:CYCS
Synonyms:CYC
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 105104Cytochrome cPRO_0000108211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication
Modified residuei49 – 491PhosphotyrosineBy similarity
Modified residuei98 – 981PhosphotyrosineBy similarity

Post-translational modificationi

Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00011.
PRIDEiP00011.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000004225.

Structurei

3D structure databases

ProteinModelPortaliP00011.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

eggNOGiCOG3474.
GeneTreeiENSGT00390000009405.
HOGENOMiHOG000009762.
HOVERGENiHBG003023.
InParanoidiP00011.
KOiK08738.
OMAiRDYLHNP.
OrthoDBiEOG761BWX.
TreeFamiTF300226.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDVEKGKKI FVQKCAQCHT VEKGGKHKTG PNLHGLFGRK TGQAPGFSYT
60 70 80 90 100
DANKNKGITW GEETLMEYLE NPKKYIPGTK MIFAGIKKTG ERADLIAYLK

KATKE
Length:105
Mass (Da):11,633
Last modified:January 23, 2007 - v2
Checksum:iB9670628E8E00483
GO

Sequence databases

PIRiA00010. CCDG.
RefSeqiNP_001183974.1. NM_001197045.1.
UniGeneiCfa.379.

Genome annotation databases

EnsembliENSCAFT00000004569; ENSCAFP00000004225; ENSCAFG00000002856.
GeneIDi475258.
KEGGicfa:475258.

Cross-referencesi

Web resourcesi

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Sequence databases

PIRiA00010. CCDG.
RefSeqiNP_001183974.1. NM_001197045.1.
UniGeneiCfa.379.

3D structure databases

ProteinModelPortaliP00011.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000004225.

Proteomic databases

PaxDbiP00011.
PRIDEiP00011.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000004569; ENSCAFP00000004225; ENSCAFG00000002856.
GeneIDi475258.
KEGGicfa:475258.

Organism-specific databases

CTDi54205.

Phylogenomic databases

eggNOGiCOG3474.
GeneTreeiENSGT00390000009405.
HOGENOMiHOG000009762.
HOVERGENiHBG003023.
InParanoidiP00011.
KOiK08738.
OMAiRDYLHNP.
OrthoDBiEOG761BWX.
TreeFamiTF300226.

Enzyme and pathway databases

ReactomeiREACT_275643. Formation of apoptosome.
REACT_284445. Respiratory electron transport.
REACT_288409. Transcriptional activation of mitochondrial biogenesis.
REACT_310683. Detoxification of Reactive Oxygen Species.
REACT_332058. Activation of caspases through apoptosome-mediated cleavage.
REACT_346812. Release of apoptotic factors from the mitochondria.
REACT_359221. TP53 Regulates Metabolic Genes.

Miscellaneous databases

NextBioi20851130.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR002327. Cyt_c_1A/1B.
[Graphical view]
PANTHERiPTHR11961. PTHR11961. 1 hit.
PfamiPF00034. Cytochrom_C. 1 hit.
[Graphical view]
PRINTSiPR00604. CYTCHRMECIAB.
SUPFAMiSSF46626. SSF46626. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Amino acid sequence of dog heart cytochrome c."
    McDowall M.A., Smith E.L.
    J. Biol. Chem. 240:4635-4647(1965) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-105, ACETYLATION AT GLY-2.

Entry informationi

Entry nameiCYC_CANFA
AccessioniPrimary (citable) accession number: P00011
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.