ID   NU4M_RHISA              Reviewed;         433 AA.
AC   O99825;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   27-MAR-2024, entry version 80.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4;
OS   Rhipicephalus sanguineus (Brown dog tick) (Ixodes sanguineus).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Rhipicephalinae;
OC   Rhipicephalus; Rhipicephalus.
OX   NCBI_TaxID=34632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9866211; DOI=10.1093/oxfordjournals.molbev.a025903;
RA   Black W.C. IV, Roehrdanz R.L.;
RT   "Mitochondrial gene order is not conserved in arthropods: prostriate and
RT   metastriate tick mitochondrial genomes.";
RL   Mol. Biol. Evol. 15:1772-1785(1998).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; AF081829; AAD05526.1; -; Genomic_DNA.
DR   PIR; T11162; T11162.
DR   RefSeq; NP_008519.1; NC_002074.1.
DR   AlphaFoldDB; O99825; -.
DR   SMR; O99825; -.
DR   EnsemblMetazoa; GeneID_808367_df_mr; NP_008519.1; GeneID_808367.
DR   GeneID; 808367; -.
DR   KEGG; rsan:808367; -.
DR   CTD; 4538; -.
DR   OrthoDB; 1946151at2759; -.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4; 1.
DR   PANTHER; PTHR43507:SF20; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..433
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117982"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        80..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        234..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..281
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        366..386
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   433 AA;  50758 MW;  93663B781633CD04 CRC64;
     MLMSVSILCL FFLINPYQIM IYLMIMTLFL LAKGLINNAE SFINFFFFDL MSLSMVILTV
     WISILMIMAS KSNNNYKNKI FNFYLLLMMN LLFICFMLEN LLMFYLFFEA VLFPIILMIS
     GWGSQPERIQ AGFYMLMYTV FGSLPLLILM LLKNQSLSII FNEWLFNEMG FIFFLMILGF
     LVKIPMFLFH LWLPKAHVEA PRAGSMILAG VLLKLGFYGL YRFKSFFFLD LLKFSFVLIV
     ISMWGAVLIS IFCLYQNDIK SLIAYSSVSH MGITLAGCVT FQLHTSFGML MMMIGHGLCS
     SGLFCLKNMI YERLHTRSIM MIKGMINFPN LSMWWFLFSI INMSAPMTMN LFGELFLGLG
     LMKYSLLLSL PVMMMIFLSA CYSMFMYSYI NHGQSWMIFS NKMISMREYY LMLLHIIPMI
     MWFLKINFFM KWI
//