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Protein

Minor histocompatibility protein HB-1

Gene

HMHB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Precursor of the histocomplatibility antigen HB-1. More generally, minor histocomplatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and its expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocomplatibility antigen in HLA-matched sibling marrow transplants. HB-1 is presented on the cell surface by MHC class I HLA-B44. This complex specifically elicits donor-cytotoxic T lymphocyte (CTL) reactivity in B-cell acute lymphoblastic leukemia (B-ALL) after treatment by HLA-identical allogenic bone marrow transplantation (BMT). It induces cell recognition and lysis by CTL. However, HB-1 restricted expression in B-ALL cells and not in normal tissues may allow a specific CTL reactivity against B-ALL without the risk of evoking graft-versus-host disease.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Minor histocompatibility protein HB-1
Cleaved into the following chain:
Gene namesi
Name:HMHB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:29677. HMHB1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi9 – 91E → A: Decreased CTL recognition. 1 Publication
Mutagenesisi10 – 101E → A: Decreased CTL recognition. 1 Publication
Mutagenesisi11 – 111K → A: Complete loss of CTL recognition. 1 Publication
Mutagenesisi12 – 121R → A: Complete loss of CTL recognition. 1 Publication
Mutagenesisi13 – 131G → A: Complete loss of CTL recognition. 1 Publication
Mutagenesisi14 – 141S → A: Complete loss of CTL recognition. 1 Publication
Mutagenesisi15 – 151L → A: Complete loss of CTL recognition. 1 Publication
Mutagenesisi16 – 161H → A: Complete loss of CTL recognition. 1 Publication
Mutagenesisi16 – 161H → R: CTL recognition. 1 Publication
Mutagenesisi17 – 171V → A: Decreased CTL recognition. 1 Publication
Mutagenesisi18 – 181W → A: Complete loss of CTL recognition. 1 Publication

Organism-specific databases

PharmGKBiPA147357973.

Polymorphism and mutation databases

BioMutaiHMHB1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4141Minor histocompatibility protein HB-1PRO_0000330317Add
BLAST
Peptidei9 – 1810Minor histocompatibility antigen HB-1PRO_0000330318

Proteomic databases

PaxDbiO97980.
PRIDEiO97980.

PTM databases

PhosphoSiteiO97980.

Expressioni

Tissue specificityi

Expressed in acute lymphoblastic leukemia B-cells and Epstein-Barr virus-transformed B-cells.2 Publications

Gene expression databases

BgeeiO97980.
CleanExiHS_HMHB1.
GenevisibleiO97980. HS.

Organism-specific databases

HPAiHPA060308.

Interactioni

Subunit structurei

HB-1 forms a complex with MHC class I HLA-B44.

Protein-protein interaction databases

STRINGi9606.ENSP00000289448.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 179Loss of recognition by cytotoxic T lymphocyte (CTL)

Phylogenomic databases

eggNOGiENOG410IT4Y. Eukaryota.
ENOG4110PZA. LUCA.
HOGENOMiHOG000112918.
InParanoidiO97980.
OrthoDBiEOG7HXCV7.
PhylomeDBiO97980.
TreeFamiTF341229.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
MEEQPECREE KRGSLHVWKS ELVEVEDDVY LRHSSSLTYR L
Length:41
Mass (Da):4,965
Last modified:May 1, 1999 - v1
Checksum:iCF81D8EB0AE6F206
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti16 – 161H → Y in allele HB-1Y; loss of CTL recognition for epitope HB-1. No influence on HLA-B/HLA-B44 binding, nor on the processing by the proteasome. 1 Publication
Corresponds to variant rs161557 [ dbSNP | Ensembl ].
VAR_042698

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103884 mRNA. Translation: AAC78642.1.
BC104412 mRNA. Translation: AAI04413.1.
BC104413 mRNA. Translation: AAI04414.1.
AF233094 Genomic DNA. Translation: AAF81110.1.
CCDSiCCDS43376.1.
RefSeqiNP_067005.1. NM_021182.1.
UniGeneiHs.158320.

Genome annotation databases

EnsembliENST00000289448; ENSP00000289448; ENSG00000158497.
GeneIDi57824.
KEGGihsa:57824.
UCSCiuc003lnj.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF103884 mRNA. Translation: AAC78642.1.
BC104412 mRNA. Translation: AAI04413.1.
BC104413 mRNA. Translation: AAI04414.1.
AF233094 Genomic DNA. Translation: AAF81110.1.
CCDSiCCDS43376.1.
RefSeqiNP_067005.1. NM_021182.1.
UniGeneiHs.158320.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000289448.

PTM databases

PhosphoSiteiO97980.

Polymorphism and mutation databases

BioMutaiHMHB1.

Proteomic databases

PaxDbiO97980.
PRIDEiO97980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000289448; ENSP00000289448; ENSG00000158497.
GeneIDi57824.
KEGGihsa:57824.
UCSCiuc003lnj.5. human.

Organism-specific databases

CTDi57824.
GeneCardsiHMHB1.
HGNCiHGNC:29677. HMHB1.
HPAiHPA060308.
MIMi609961. gene.
neXtProtiNX_O97980.
PharmGKBiPA147357973.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IT4Y. Eukaryota.
ENOG4110PZA. LUCA.
HOGENOMiHOG000112918.
InParanoidiO97980.
OrthoDBiEOG7HXCV7.
PhylomeDBiO97980.
TreeFamiTF341229.

Miscellaneous databases

GenomeRNAii57824.
NextBioi64802.
PROiO97980.
SOURCEiSearch...

Gene expression databases

BgeeiO97980.
CleanExiHS_HMHB1.
GenevisibleiO97980. HS.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human minor histocompatibility antigen specific for B cell acute lymphoblastic leukemia."
    Dolstra H., Fredrix H., Maas F., Coulie P.G., Brasseur F., Mensink E., Adema G.J., de Witte T.M., Figdor C.G., van de Wiel-van Kemenade E.
    J. Exp. Med. 189:301-308(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 9-18, TISSUE SPECIFICITY, FUNCTION, VARIANT TYR-16.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Genomic identification of the HB-1 gene."
    Balas A., Aviles M.J., Vicario J.L.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
  4. "Recognition of a B cell leukemia-associated minor histocompatibility antigen by CTL."
    Dolstra H., Fredrix H., Preijers F., Goulmy E., Figdor C.G., de Witte T.M., van de Wiel-van Kemenade E.
    J. Immunol. 158:560-565(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. "Bi-directional allelic recognition of the human minor histocompatibility antigen HB-1 by cytotoxic T lymphocytes."
    Dolstra H., de Rijke B., Fredrix H., Balas A., Maas F., Scherpen F., Aviles M.J., Vicario J.L., Beekman N.J., Ossendorp F., de Witte T.M., van de Wiel-van Kemenade E.
    Eur. J. Immunol. 32:2748-2758(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HLA-B44, MUTAGENESIS OF GLU-9; GLU-10; LYS-11; ARG-12; GLY-13; SER-14; LEU-15; HIS-16; VAL-17 AND TRP-18.
  6. "Minor histocompatibility antigens -- big in tumour therapy."
    Spierings E., Wieles B., Goulmy E.
    Trends Immunol. 25:56-60(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHMHB1_HUMAN
AccessioniPrimary (citable) accession number: O97980
Secondary accession number(s): Q9MY25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 1, 1999
Last modified: March 16, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

This sequence containing mHag HB-1 may not represent the full-length protein, because functional domains are absent in this short gene product.1 Publication
PubMed:9892612 postulated that the initiator methionine is coded by a non-canonical CTG leucine codon.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.