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O97972

- INMT_RABIT

UniProt

O97972 - INMT_RABIT

Protein

Indolethylamine N-methyltransferase

Gene

INMT

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the N-methylation of tryptamine and structurally related compounds By similarity. Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine.
    S-adenosyl-L-methionine + dimethyl sulfide = S-adenosyl-L-homocysteine + trimethylsulfonium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201S-adenosyl-L-methionineBy similarity
    Binding sitei25 – 251S-adenosyl-L-methionineBy similarity
    Binding sitei69 – 691S-adenosyl-L-methionineBy similarity
    Binding sitei85 – 851S-adenosyl-L-methionineBy similarity
    Binding sitei90 – 901S-adenosyl-L-methionineBy similarity
    Binding sitei163 – 1631S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. amine N-methyltransferase activity Source: UniProtKB
    2. thioether S-methyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. amine metabolic process Source: UniProtKB
    2. methylation Source: UniProtKB
    3. response to toxic substance Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Detoxification

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    SABIO-RKO97972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Indolethylamine N-methyltransferase (EC:2.1.1.49, EC:2.1.1.96)
    Short name:
    Indolamine N-methyltransferase
    Alternative name(s):
    Aromatic alkylamine N-methyltransferase
    Short name:
    Amine N-methyltransferase
    Short name:
    Arylamine N-methyltransferase
    Thioether S-methyltransferase
    Gene namesi
    Name:INMT
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 263263Indolethylamine N-methyltransferasePRO_0000159715Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131N6-succinyllysineBy similarity
    Modified residuei96 – 961N6-succinyllysineBy similarity

    Expressioni

    Tissue specificityi

    Highly expressed in lung, also detected in liver and at very low levels in brain.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000021686.

    Structurei

    3D structure databases

    ProteinModelPortaliO97972.
    SMRiO97972. Positions 5-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 642S-adenosyl-L-methionine bindingBy similarity
    Regioni142 – 1432S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG71857.
    HOGENOMiHOG000013229.
    HOVERGENiHBG000797.
    OMAiAMECACC.
    OrthoDBiEOG7673B9.
    TreeFamiTF313114.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025817. Amine_MeTrfase.
    IPR025820. NNMT/PNMT/TEMT_CS.
    IPR000940. NNMT_TEMT_trans.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10867. PTHR10867. 1 hit.
    PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000384. PNMTase. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
    PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O97972-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGGFTGGDE YQKHFLPRDY LNTYYSFQSG PSPEAEMLKF NLECLHKTFG    50
    PGGLQGDTLI DIGSGPTIYQ VLAACESFKD ITLSDFTDRN REELAKWLKK 100
    EPGAYDWTPA LKFACELEGN SGRWQEKAEK LRATVKRVLK CDANLSNPLT 150
    PVVLPPADCV LTLLAMECAC CSLDAYRAAL RNLASLLKPG GHLVTTVTLQ 200
    LSSYMVGERE FSCVALEKEE VEQAVLDAGF DIEQLLYSPQ SYSASTAPNR 250
    GVCFLVARKK PGS 263
    Length:263
    Mass (Da):28,955
    Last modified:May 1, 1999 - v1
    Checksum:i42F368414BE8B459
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077828, AF077827 Genomic DNA. Translation: AAC97492.1.
    AF077826 mRNA. Translation: AAC97491.1.
    RefSeqiNP_001075512.1. NM_001082043.1.
    UniGeneiOcu.2318.

    Genome annotation databases

    GeneIDi100008695.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF077828 , AF077827 Genomic DNA. Translation: AAC97492.1 .
    AF077826 mRNA. Translation: AAC97491.1 .
    RefSeqi NP_001075512.1. NM_001082043.1.
    UniGenei Ocu.2318.

    3D structure databases

    ProteinModelPortali O97972.
    SMRi O97972. Positions 5-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000021686.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100008695.

    Organism-specific databases

    CTDi 11185.

    Phylogenomic databases

    eggNOGi NOG71857.
    HOGENOMi HOG000013229.
    HOVERGENi HBG000797.
    OMAi AMECACC.
    OrthoDBi EOG7673B9.
    TreeFami TF313114.

    Enzyme and pathway databases

    SABIO-RK O97972.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR025817. Amine_MeTrfase.
    IPR025820. NNMT/PNMT/TEMT_CS.
    IPR000940. NNMT_TEMT_trans.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10867. PTHR10867. 1 hit.
    Pfami PF01234. NNMT_PNMT_TEMT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000384. PNMTase. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS01100. NNMT_PNMT_TEMT. 1 hit.
    PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rabbit lung indolethylamine N-methyltransferase. cDNA and gene cloning and characterization."
      Thompson M.A., Weinshilboum R.M.
      J. Biol. Chem. 273:34502-34510(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 1-129, CHARACTERIZATION.
      Tissue: Lung.
    2. "Purification and molecular properties of rabbit lung indolamine N-methyltransferase."
      Irace G., Colonna G., Camardella M., Della Pietra G., Porta R.
      Biochemistry 21:1464-1470(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Tissue: Lung.

    Entry informationi

    Entry nameiINMT_RABIT
    AccessioniPrimary (citable) accession number: O97972
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3