Reviewed, UniProtKB/Swiss-Prot O97972 (INMT_RABIT)

Names and origin

Protein names

Recommended name:
    Indolethylamine N-methyltransferase
      Short name=Indolamine N-methyltransferase
    EC=2.1.1.49
Alternative name(s):
    Aromatic alkylamine N-methyltransferase
      Short name=Arylamine N-methyltransferase
      Short name=Amine N-methyltransferase

Gene names

Name:

INMT

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the N-methylation of tryptamine and structurally related compounds Potential.
Catalytic activity	S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Highly expressed in lung, also detected in liver and at very low levels in brain.
Sequence similarities	Belongs to the NNMT/PNMT/TEMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Indolethylamine N-methyltransferase		PRO_0000159715
Regions
	Region	63 – 64	2	S-adenosyl-L-methionine binding By similarity
	Region	142 – 143	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	20	1	S-adenosyl-L-methionine By similarity
	Binding site	25	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine By similarity
	Binding site	85	1	S-adenosyl-L-methionine By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	163	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O97972-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 42F368414BE8B459	FASTA	263	28,955
	10 20 30 40 50 60 MEGGFTGGDE YQKHFLPRDY LNTYYSFQSG PSPEAEMLKF NLECLHKTFG PGGLQGDTLI 70 80 90 100 110 120 DIGSGPTIYQ VLAACESFKD ITLSDFTDRN REELAKWLKK EPGAYDWTPA LKFACELEGN 130 140 150 160 170 180 SGRWQEKAEK LRATVKRVLK CDANLSNPLT PVVLPPADCV LTLLAMECAC CSLDAYRAAL 190 200 210 220 230 240 RNLASLLKPG GHLVTTVTLQ LSSYMVGERE FSCVALEKEE VEQAVLDAGF DIEQLLYSPQ 250 260 SYSASTAPNR GVCFLVARKK PGS

References

[1]	"Rabbit lung indolethylamine N-methyltransferase. cDNA and gene cloning and characterization." Thompson M.A., Weinshilboum R.M. J. Biol. Chem. 273:34502-34510(1998) [PubMed: 9852119] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 1-129, CHARACTERIZATION. Tissue: Lung.
[2]	"Purification and molecular properties of rabbit lung indolamine N-methyltransferase." Irace G., Colonna G., Camardella M., Della Pietra G., Porta R. Biochemistry 21:1464-1470(1982) [PubMed: 7074100] [Abstract] Cited for: CHARACTERIZATION. Tissue: Lung.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF077828, AF077827 Genomic DNA. Translation: AAC97492.1. AF077826 mRNA. Translation: AAC97491.1.
RefSeq	NP_001075512.1.
UniGene	Ocu.2318
3D structure databases
HSSP	HSSP built from PDB template 1HNN based on UniProtKB P11086.
SMR	O97972. Positions 5-261.
ModBase	Search...
Genome annotation databases
GeneID	100008695.
Phylogenomic databases
HOVERGEN	O97972.
Enzyme and pathway databases
BRENDA	2.1.1.49. 255.
Family and domain databases
InterPro	IPR000940. NNMT_TEMT_trans. [Graphical view]
PANTHER	PTHR10867. NNMT_TEMT_trans. 1 hit.
Pfam	PF01234. NNMT_PNMT_TEMT. 1 hit. [Graphical view]
PIRSF	PIRSF000384. PNMTase. 1 hit.
PROSITE	PS01100. NNMT_PNMT_TEMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INMT_RABIT

Accession

Primary (citable) accession number: O97972

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)