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O97972

- INMT_RABIT

UniProt

O97972 - INMT_RABIT

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Protein

Indolethylamine N-methyltransferase

Gene

INMT

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the N-methylation of tryptamine and structurally related compounds (By similarity). Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine.
S-adenosyl-L-methionine + dimethyl sulfide = S-adenosyl-L-homocysteine + trimethylsulfonium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201S-adenosyl-L-methionineBy similarity
Binding sitei25 – 251S-adenosyl-L-methionineBy similarity
Binding sitei69 – 691S-adenosyl-L-methionineBy similarity
Binding sitei85 – 851S-adenosyl-L-methionineBy similarity
Binding sitei90 – 901S-adenosyl-L-methionineBy similarity
Binding sitei163 – 1631S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. amine N-methyltransferase activity Source: UniProtKB
  2. thioether S-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. amine metabolic process Source: UniProtKB
  2. methylation Source: UniProtKB
  3. response to toxic substance Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Detoxification

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

SABIO-RKO97972.

Names & Taxonomyi

Protein namesi
Recommended name:
Indolethylamine N-methyltransferase (EC:2.1.1.49, EC:2.1.1.96)
Short name:
Indolamine N-methyltransferase
Alternative name(s):
Aromatic alkylamine N-methyltransferase
Short name:
Amine N-methyltransferase
Short name:
Arylamine N-methyltransferase
Thioether S-methyltransferase
Gene namesi
Name:INMT
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Indolethylamine N-methyltransferasePRO_0000159715Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131N6-succinyllysineBy similarity
Modified residuei96 – 961N6-succinyllysineBy similarity

Expressioni

Tissue specificityi

Highly expressed in lung, also detected in liver and at very low levels in brain.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000021686.

Structurei

3D structure databases

ProteinModelPortaliO97972.
SMRiO97972. Positions 5-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 642S-adenosyl-L-methionine bindingBy similarity
Regioni142 – 1432S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG71857.
HOGENOMiHOG000013229.
HOVERGENiHBG000797.
InParanoidiO97972.
OMAiAMECACC.
OrthoDBiEOG7673B9.
TreeFamiTF313114.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025817. Amine_MeTrfase.
IPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10867. PTHR10867. 1 hit.
PfamiPF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view]
PIRSFiPIRSF000384. PNMTase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O97972-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGGFTGGDE YQKHFLPRDY LNTYYSFQSG PSPEAEMLKF NLECLHKTFG
60 70 80 90 100
PGGLQGDTLI DIGSGPTIYQ VLAACESFKD ITLSDFTDRN REELAKWLKK
110 120 130 140 150
EPGAYDWTPA LKFACELEGN SGRWQEKAEK LRATVKRVLK CDANLSNPLT
160 170 180 190 200
PVVLPPADCV LTLLAMECAC CSLDAYRAAL RNLASLLKPG GHLVTTVTLQ
210 220 230 240 250
LSSYMVGERE FSCVALEKEE VEQAVLDAGF DIEQLLYSPQ SYSASTAPNR
260
GVCFLVARKK PGS
Length:263
Mass (Da):28,955
Last modified:May 1, 1999 - v1
Checksum:i42F368414BE8B459
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077828, AF077827 Genomic DNA. Translation: AAC97492.1.
AF077826 mRNA. Translation: AAC97491.1.
RefSeqiNP_001075512.1. NM_001082043.1.
UniGeneiOcu.2318.

Genome annotation databases

GeneIDi100008695.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077828 , AF077827 Genomic DNA. Translation: AAC97492.1 .
AF077826 mRNA. Translation: AAC97491.1 .
RefSeqi NP_001075512.1. NM_001082043.1.
UniGenei Ocu.2318.

3D structure databases

ProteinModelPortali O97972.
SMRi O97972. Positions 5-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000021686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100008695.

Organism-specific databases

CTDi 11185.

Phylogenomic databases

eggNOGi NOG71857.
HOGENOMi HOG000013229.
HOVERGENi HBG000797.
InParanoidi O97972.
OMAi AMECACC.
OrthoDBi EOG7673B9.
TreeFami TF313114.

Enzyme and pathway databases

SABIO-RK O97972.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR025817. Amine_MeTrfase.
IPR025820. NNMT/PNMT/TEMT_CS.
IPR000940. NNMT_TEMT_trans.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10867. PTHR10867. 1 hit.
Pfami PF01234. NNMT_PNMT_TEMT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000384. PNMTase. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS01100. NNMT_PNMT_TEMT. 1 hit.
PS51681. SAM_MT_NNMT_PNMT_TEMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rabbit lung indolethylamine N-methyltransferase. cDNA and gene cloning and characterization."
    Thompson M.A., Weinshilboum R.M.
    J. Biol. Chem. 273:34502-34510(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 1-129, CHARACTERIZATION.
    Tissue: Lung.
  2. "Purification and molecular properties of rabbit lung indolamine N-methyltransferase."
    Irace G., Colonna G., Camardella M., Della Pietra G., Porta R.
    Biochemistry 21:1464-1470(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Tissue: Lung.

Entry informationi

Entry nameiINMT_RABIT
AccessioniPrimary (citable) accession number: O97972
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3