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O97921

- PTGDS_HORSE

UniProt

O97921 - PTGDS_HORSE

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Protein

Prostaglandin-H2 D-isomerase

Gene
PTGDS
Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system By similarity.

Catalytic activityi

(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Nucleophile By similarity

GO - Molecular functioni

  1. prostaglandin-D synthase activity Source: UniProtKB
  2. retinoid binding Source: UniProtKB
  3. small molecule binding Source: InterPro
  4. transporter activity Source: UniProtKB

GO - Biological processi

  1. prostaglandin biosynthetic process Source: UniProtKB
  2. regulation of circadian sleep/wake cycle, sleep Source: UniProtKB
  3. transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin-H2 D-isomerase (EC:5.3.99.2)
Alternative name(s):
Glutathione-independent PGD synthase
Lipocalin-type prostaglandin-D synthase
Prostaglandin-D2 synthase
Short name:
PGD2 synthase
Short name:
PGDS
Short name:
PGDS2
Gene namesi
Name:PTGDS
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

Rough endoplasmic reticulum By similarity. Nucleus membrane By similarity. Golgi apparatus By similarity. Cytoplasmperinuclear region By similarity. Secreted By similarity
Note: Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted By similarity.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. extracellular space Source: UniProtKB
  3. Golgi apparatus Source: UniProtKB
  4. nuclear membrane Source: UniProtKB-SubCell
  5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  6. rough endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 By similarityAdd
BLAST
Chaini25 – 194170Prostaglandin-H2 D-isomerasePRO_0000017944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251Pyrrolidone carboxylic acid By similarity
Glycosylationi51 – 511N-linked (GlcNAc...) By similarity
Glycosylationi78 – 781N-linked (GlcNAc...) By similarity
Disulfide bondi89 ↔ 189 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

In the male reproductive system, it is expressed in the testis and epididymis, and is secreted into the seminal fluid.1 Publication

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi9796.ENSECAP00000007190.

Structurei

3D structure databases

ProteinModelPortaliO97921.
SMRiO97921. Positions 24-192.

Family & Domainsi

Domaini

Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG45731.
HOVERGENiHBG106490.
KOiK01830.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002972. PstgldnD_synth.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00179. LIPOCALIN.
PR01254. PGNDSYNTHASE.
SUPFAMiSSF50814. SSF50814. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97921-1 [UniParc]FASTAAdd to Basket

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MAASHTLWMG LVLLGVLGVL QTRAQAQPSL QPNFQQDKFL GRWFTSGLAS    50
NSSWFREKKK VLSMCTSVVA PTADGGFNLT STFLRKDQCE TRTLLLQPAG 100
PPGCYSYTSP HWGMVHEVSV VETDYEEYAL LYTHAESTKG LGGQDFRMAT 150
LYSRVQSPRP EVKEKFSTFA KAQGFTEDAI VFLPQTDKCM EEHN 194
Length:194
Mass (Da):21,669
Last modified:May 1, 1999 - v1
Checksum:iC6E271A540877994
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1217VHEVSVV → PHEMSMM AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ133469 mRNA. Translation: CAB38173.1.
RefSeqiNP_001075337.1. NM_001081868.1.
UniGeneiEca.12658.

Genome annotation databases

GeneIDi100067921.
KEGGiecb:100067921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ133469 mRNA. Translation: CAB38173.1 .
RefSeqi NP_001075337.1. NM_001081868.1.
UniGenei Eca.12658.

3D structure databases

ProteinModelPortali O97921.
SMRi O97921. Positions 24-192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000007190.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100067921.
KEGGi ecb:100067921.

Organism-specific databases

CTDi 5730.

Phylogenomic databases

eggNOGi NOG45731.
HOVERGENi HBG106490.
KOi K01830.

Family and domain databases

Gene3Di 2.40.128.20. 1 hit.
InterProi IPR012674. Calycin.
IPR011038. Calycin-like.
IPR002345. Lipocalin.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
IPR002972. PstgldnD_synth.
[Graphical view ]
Pfami PF00061. Lipocalin. 1 hit.
[Graphical view ]
PRINTSi PR00179. LIPOCALIN.
PR01254. PGNDSYNTHASE.
SUPFAMi SSF50814. SSF50814. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Mammalian lipocalin-type prostaglandin D2 synthase in the fluids of the male genital tract: putative biochemical and physiological functions."
    Fouchecourt S., Charpigny G., Reinaud P., Dumont P., Dacheux J.-L.
    Biol. Reprod. 66:458-467(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Glutathione-independent prostaglandin D2 synthase in ram and stallion epididymal fluids: origin and regulation."
    Fouchecourt S., Dacheux F., Dacheux J.-L.
    Biol. Reprod. 60:558-566(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 108-122, TISSUE SPECIFICITY.

Entry informationi

Entry nameiPTGDS_HORSE
AccessioniPrimary (citable) accession number: O97921
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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