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O97859

- NEUR3_BOVIN

UniProt

O97859 - NEUR3_BOVIN

Protein

Sialidase-3

Gene

NEU3

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.1 Publication

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251SubstrateBy similarity
    Binding sitei45 – 451SubstrateBy similarity
    Active sitei50 – 501Proton acceptorBy similarity
    Binding sitei179 – 1791SubstrateBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei225 – 2251SubstrateBy similarity
    Binding sitei245 – 2451SubstrateSequence Analysis
    Binding sitei341 – 3411SubstrateBy similarity
    Active sitei371 – 3711NucleophileBy similarity
    Active sitei388 – 3881Sequence Analysis

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. ganglioside catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid degradation, Lipid metabolism

    Protein family/group databases

    CAZyiGH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase-3 (EC:3.2.1.18)
    Alternative name(s):
    Ganglioside sialidase
    Membrane sialidase
    N-acetyl-alpha-neuraminidase 3
    Gene namesi
    Name:NEU3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Sialidase-3PRO_0000208902Add
    BLAST

    Proteomic databases

    PRIDEiO97859.

    Expressioni

    Tissue specificityi

    Expressed in brain.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000036593.

    Structurei

    3D structure databases

    ProteinModelPortaliO97859.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati129 – 14012BNR 1Add
    BLAST
    Repeati203 – 21412BNR 2Add
    BLAST
    Repeati254 – 26512BNR 3Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi24 – 274FRIP motif

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 3 BNR repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG84455.
    HOGENOMiHOG000233778.
    HOVERGENiHBG052608.
    InParanoidiO97859.
    KOiK12357.

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR026944. Sialidase-3.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF7. PTHR10628:SF7. 1 hit.
    SUPFAMiSSF50939. SSF50939. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    O97859-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEVTSCSFS SPLFQQEDKR GVTYRIPALI YVPPAHTFLA FAEKRSSSKD    50
    EDALHLVLRR GLRTGQSVQW EPLKSLMKAT LPGHRTMNPC PVWERKSGYV 100
    YLFFICVQGH VTERQQIMSG RNPARLCFIC SQDAGYSWSD VRDLTEEVIG 150
    PEVTHWATFA VGPGHGIQLQ SGRLIIPAYA YYIPFWFFCF RLPYRARPHS 200
    LMIYSDDLGA TWHHGRLIKP MVTVECEVAE VIGKAGHPVL YCSARTPNRH 250
    RAEALSIDHG ECFQKPVLSH QLCEPPHGCQ GSVVSFCPLE IPGGCQDLAG 300
    EDAPAIQQSP LLCSSVRPEP EAGTLSESWL LYSHPTNKKR RVDLGIYLNQ 350
    SPLEAACWSR PWILHCGPCG YSDLAALENE GLFGCLFECG TKQECEQIAF 400
    RLFTDREILS HVQGDCSTPG MNSEPSKK 428
    Length:428
    Mass (Da):47,917
    Last modified:May 1, 1999 - v1
    Checksum:i418B34F3245A8F21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008184 mRNA. Translation: BAA75071.1.
    RefSeqiNP_776547.2. NM_174122.3.
    UniGeneiBt.477.

    Genome annotation databases

    GeneIDi281349.
    KEGGibta:281349.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB008184 mRNA. Translation: BAA75071.1 .
    RefSeqi NP_776547.2. NM_174122.3.
    UniGenei Bt.477.

    3D structure databases

    ProteinModelPortali O97859.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000036593.

    Protein family/group databases

    CAZyi GH33. Glycoside Hydrolase Family 33.

    Proteomic databases

    PRIDEi O97859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281349.
    KEGGi bta:281349.

    Organism-specific databases

    CTDi 10825.

    Phylogenomic databases

    eggNOGi NOG84455.
    HOGENOMi HOG000233778.
    HOVERGENi HBG052608.
    InParanoidi O97859.
    KOi K12357.

    Miscellaneous databases

    NextBioi 20805357.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR026944. Sialidase-3.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF7. PTHR10628:SF7. 1 hit.
    SUPFAMi SSF50939. SSF50939. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a plasma membrane-associated sialidase specific for gangliosides."
      Miyagi T., Wada T., Iwamatsu A., Hata K., Yoshikawa Y., Tokuyama S., Sawada M.
      J. Biol. Chem. 274:5004-5011(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Tissue: Brain.

    Entry informationi

    Entry nameiNEUR3_BOVIN
    AccessioniPrimary (citable) accession number: O97859
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3