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Reviewed, UniProtKB/Swiss-Prot O97859 (NEUR3_BOVIN)

Last modified June 16, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sialidase-3
    EC=3.2.1.18
Alternative name(s):
    Membrane sialidase
    Ganglioside sialidase
    N-acetyl-alpha-neuraminidase 3
Gene names
Name: NEU3
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed in brain.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 3 BNR repeats.

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Ontologies

Keywords
   Cellular componentMembrane
   DomainRepeat
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionexo-alpha-sialidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Sialidase-3
PRO_0000208902

Regions

Repeat129 – 14012BNR 1
Repeat203 – 21412BNR 2
Repeat254 – 26512BNR 3
Motif24 – 274FRIP motif

Sites

Active site501Proton acceptor By similarity
Active site3711Nucleophile By similarity
Active site3881 Potential
Binding site251Substrate By similarity
Binding site2451Substrate Potential
Binding site3411Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
O97859-1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 418B34F3245A8F21

FASTA42847,917
        10         20         30         40         50         60 
MEEVTSCSFS SPLFQQEDKR GVTYRIPALI YVPPAHTFLA FAEKRSSSKD EDALHLVLRR 

        70         80         90        100        110        120 
GLRTGQSVQW EPLKSLMKAT LPGHRTMNPC PVWERKSGYV YLFFICVQGH VTERQQIMSG 

       130        140        150        160        170        180 
RNPARLCFIC SQDAGYSWSD VRDLTEEVIG PEVTHWATFA VGPGHGIQLQ SGRLIIPAYA 

       190        200        210        220        230        240 
YYIPFWFFCF RLPYRARPHS LMIYSDDLGA TWHHGRLIKP MVTVECEVAE VIGKAGHPVL 

       250        260        270        280        290        300 
YCSARTPNRH RAEALSIDHG ECFQKPVLSH QLCEPPHGCQ GSVVSFCPLE IPGGCQDLAG 

       310        320        330        340        350        360 
EDAPAIQQSP LLCSSVRPEP EAGTLSESWL LYSHPTNKKR RVDLGIYLNQ SPLEAACWSR 

       370        380        390        400        410        420 
PWILHCGPCG YSDLAALENE GLFGCLFECG TKQECEQIAF RLFTDREILS HVQGDCSTPG 


MNSEPSKK

« Hide

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References

[1]"Molecular cloning and characterization of a plasma membrane-associated sialidase specific for gangliosides."
Miyagi T., Wada T., Iwamatsu A., Hata K., Yoshikawa Y., Tokuyama S., Sawada M.
J. Biol. Chem. 274:5004-5011(1999) [PubMed: 9988745] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
Tissue: Brain.

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Cross-references

Sequence databases

AB008184 mRNA. Translation: BAA75071.1.
IPIIPI00687458.
RefSeqNP_776547.1.
UniGeneBt.477

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Genome annotation databases

EnsemblENSBTAG00000025931. Bos taurus. [Contig view]
GeneID281349.
KEGGbta:281349.

Phylogenomic databases

HOVERGENO97859.

Enzyme and pathway databases

BRENDA3.2.1.18. 251.

Family and domain databases

ProtoNetSearch...

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Entry information

Entry nameNEUR3_BOVIN
AccessionPrimary (citable) accession number: O97859
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 1999
Last modified: June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents