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O97859 (NEUR3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase-3

EC=3.2.1.18
Alternative name(s):
Ganglioside sialidase
Membrane sialidase
N-acetyl-alpha-neuraminidase 3
Gene names
Name:NEU3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates. Ref.1

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. Ref.1

Subcellular location

Cell membrane; Peripheral membrane protein Ref.1.

Tissue specificity

Expressed in brain. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 3 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Sialidase-3
PRO_0000208902

Regions

Repeat129 – 14012BNR 1
Repeat203 – 21412BNR 2
Repeat254 – 26512BNR 3
Motif24 – 274FRIP motif

Sites

Active site501Proton acceptor By similarity
Active site3711Nucleophile By similarity
Active site3881 Potential
Binding site251Substrate By similarity
Binding site451Substrate By similarity
Binding site1791Substrate By similarity
Binding site1811Substrate By similarity
Binding site2251Substrate By similarity
Binding site2451Substrate Potential
Binding site3411Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O97859 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 418B34F3245A8F21

FASTA42847,917
        10         20         30         40         50         60 
MEEVTSCSFS SPLFQQEDKR GVTYRIPALI YVPPAHTFLA FAEKRSSSKD EDALHLVLRR 

        70         80         90        100        110        120 
GLRTGQSVQW EPLKSLMKAT LPGHRTMNPC PVWERKSGYV YLFFICVQGH VTERQQIMSG 

       130        140        150        160        170        180 
RNPARLCFIC SQDAGYSWSD VRDLTEEVIG PEVTHWATFA VGPGHGIQLQ SGRLIIPAYA 

       190        200        210        220        230        240 
YYIPFWFFCF RLPYRARPHS LMIYSDDLGA TWHHGRLIKP MVTVECEVAE VIGKAGHPVL 

       250        260        270        280        290        300 
YCSARTPNRH RAEALSIDHG ECFQKPVLSH QLCEPPHGCQ GSVVSFCPLE IPGGCQDLAG 

       310        320        330        340        350        360 
EDAPAIQQSP LLCSSVRPEP EAGTLSESWL LYSHPTNKKR RVDLGIYLNQ SPLEAACWSR 

       370        380        390        400        410        420 
PWILHCGPCG YSDLAALENE GLFGCLFECG TKQECEQIAF RLFTDREILS HVQGDCSTPG 


MNSEPSKK 

« Hide

References

[1]"Molecular cloning and characterization of a plasma membrane-associated sialidase specific for gangliosides."
Miyagi T., Wada T., Iwamatsu A., Hata K., Yoshikawa Y., Tokuyama S., Sawada M.
J. Biol. Chem. 274:5004-5011(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB008184 mRNA. Translation: BAA75071.1.
RefSeqNP_776547.2. NM_174122.3.
UniGeneBt.477.

3D structure databases

ProteinModelPortalO97859.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000036593.

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PRIDEO97859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID281349.
KEGGbta:281349.

Organism-specific databases

CTD10825.

Phylogenomic databases

eggNOGNOG84455.
HOGENOMHOG000233778.
HOVERGENHBG052608.
InParanoidO97859.
KOK12357.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF7. PTHR10628:SF7. 1 hit.
SUPFAMSSF50939. SSF50939. 2 hits.
ProtoNetSearch...

Other

NextBio20805357.

Entry information

Entry nameNEUR3_BOVIN
AccessionPrimary (citable) accession number: O97859
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 1999
Last modified: March 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries