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O97831

- LPHN1_BOVIN

UniProt

O97831 - LPHN1_BOVIN

Protein

Latrophilin-1

Gene

LPHN1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization. Receptor propably implicated in the regulation of exocytosis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei42 – 421CarbohydrateBy similarity
    Sitei837 – 8382Cleavage; by autocatalysisBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. cell adhesion molecule binding Source: UniProtKB
    3. G-protein coupled receptor activity Source: UniProtKB-KW
    4. latrotoxin receptor activity Source: UniProtKB

    GO - Biological processi

    1. calcium-mediated signaling using intracellular calcium source Source: UniProtKB
    2. heterophilic cell-cell adhesion Source: UniProtKB
    3. neuropeptide signaling pathway Source: InterPro
    4. positive regulation of synapse maturation Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Ligandi

    Lectin

    Protein family/group databases

    MEROPSiS63.013.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Latrophilin-1
    Alternative name(s):
    Calcium-independent alpha-latrotoxin receptor 1
    Short name:
    CIRL-1
    Gene namesi
    Name:LPHN1
    Synonyms:LPH1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 7

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cell projectionaxon. Cell projectiongrowth cone. Cell junctionsynapse. Cell junctionsynapsepresynaptic cell membrane. Cell junctionsynapsesynaptosome
    Note: Colocalizes with TENM2 on the cell surface, across intercellular junctions and on nerve terminals near synaptic clefts.

    GO - Cellular componenti

    1. axon Source: UniProtKB
    2. cell junction Source: UniProtKB-KW
    3. growth cone Source: UniProtKB
    4. integral component of membrane Source: UniProtKB-KW
    5. neuron projection Source: UniProtKB
    6. plasma membrane Source: UniProtKB
    7. presynaptic membrane Source: UniProtKB
    8. synapse Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424By similarityAdd
    BLAST
    Chaini25 – 14721448Latrophilin-1PRO_0000012906Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi41 ↔ 71PROSITE-ProRule annotation
    Disulfide bondi50 ↔ 128PROSITE-ProRule annotation
    Disulfide bondi83 ↔ 115PROSITE-ProRule annotation
    Disulfide bondi96 ↔ 102PROSITE-ProRule annotation
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi140 ↔ 322PROSITE-ProRule annotation
    Disulfide bondi480 ↔ 515PROSITE-ProRule annotation
    Disulfide bondi503 ↔ 532PROSITE-ProRule annotation
    Glycosylationi531 – 5311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi640 – 6401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi741 – 7411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi800 – 8001N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi801 ↔ 832PROSITE-ProRule annotation
    Glycosylationi805 – 8051N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi820 ↔ 834PROSITE-ProRule annotation
    Glycosylationi826 – 8261N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Autoproteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. This proteolytic processing takes place early in the biosynthetic pathway, either in the endoplasmic reticulum or in the early compartment of the Golgi apparatus By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiO97831.

    Expressioni

    Tissue specificityi

    Brain-specific expression but low levels are also detected in kidney, lung and spleen.1 Publication

    Interactioni

    Subunit structurei

    Forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). Interacts with syntaxin and with protein of the SHANK family via the PDZ domain By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO97831.
    SMRiO97831. Positions 29-134.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 857833ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini879 – 89214CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini914 – 9196ExtracellularSequence Analysis
    Topological domaini941 – 96323CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini985 – 100117ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1023 – 104927CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1071 – 10744ExtracellularSequence Analysis
    Topological domaini1096 – 1472377CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei858 – 87821Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei893 – 91321Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei920 – 94021Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei964 – 98421Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei1002 – 102221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei1050 – 107021Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei1075 – 109521Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 12990SUEL-type lectinPROSITE-ProRule annotationAdd
    BLAST
    Domaini139 – 398260Olfactomedin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini798 – 84952GPSPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni117 – 1204Carbohydrate bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi413 – 4164Poly-Thr
    Compositional biasi1302 – 131312Poly-ProAdd
    BLAST
    Compositional biasi1408 – 141811Poly-ProAdd
    BLAST

    Domaini

    The extracellular domain coupled to the a single transmembrane region are sufficient for full responsiveness to alpha-latrotoxin.By similarity

    Sequence similaritiesi

    Contains 1 GPS domain.PROSITE-ProRule annotation
    Contains 1 olfactomedin-like domain.PROSITE-ProRule annotation
    Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG253931.
    GeneTreeiENSGT00710000106375.
    HOGENOMiHOG000049065.
    HOVERGENiHBG052337.
    InParanoidiO97831.
    KOiK04592.

    Family and domain databases

    InterProiIPR022624. DUF3497.
    IPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR003924. GPCR_2_latrophilin.
    IPR003334. GPCR_2_latrophilin_rcpt_C.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    IPR000203. GPS.
    IPR000922. Lectin_gal-bd_dom.
    IPR003112. Olfac-like.
    [Graphical view]
    PfamiPF00002. 7tm_2. 1 hit.
    PF12003. DUF3497. 1 hit.
    PF02140. Gal_Lectin. 1 hit.
    PF01825. GPS. 1 hit.
    PF02793. HRM. 1 hit.
    PF02354. Latrophilin. 1 hit.
    PF02191. OLF. 1 hit.
    [Graphical view]
    PRINTSiPR00249. GPCRSECRETIN.
    PR01444. LATROPHILIN.
    SMARTiSM00303. GPS. 1 hit.
    SM00008. HormR. 1 hit.
    SM00284. OLF. 1 hit.
    [Graphical view]
    PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    PS50221. GPS. 1 hit.
    PS51132. OLF. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O97831-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MARLAAVLWS LCVTAILVTS ATQGLSRAGL PFGLMRRELA CEGYPIELRC     50
    PGSDVIMVEN ANYGRTDDKI CDADPFQMEN VQCYLPDAFK IMSQRCNNRT 100
    QCVVVAGSDA FPDPCPGTYK YLEVQYDCVP YKVKQKVFVC PGTLQKVLEP 150
    TSTHESEHQS GAWCKDPLQA GDRIYVMPWI PYRTDTLTEY ASWEDYVAAR 200
    HTTTYRLPNR VDGTGFVVYD GAVFYNKERT RNIVKYDLRT RIKSGETVIN 250
    TANYHDTSPY RWGGKTDIDL AVDENGLWVI YATEGNNGRL VVSQLNPYTL 300
    RFEGTWETGY DKRSASNAFM VCGVLYVLRS VYVDDDSEAA GNRVDYAFNT 350
    NANREEPVSL AFPNPYQFVS SVDYNPRDNQ LYVWNNYFVV RYSLEFGPPD 400
    PSAGPATSPP LSTTTTARPT PLTSTASPAA TTPLRRAPLT THPVGAINQL 450
    GPDLPPATAP APSTRRPPAP NLHVSPELFC EPREVRRVQW PATQQGMLVE 500
    RPCPKGTRGI ASFQCLPALG LWNPRGPDLS NCTSPWVNQV AQKIKSGENA 550
    ANIASELARH TRGSIYAGDV SSSVKLMEQL LDILDAQLQA LRPIERESAG 600
    KNYNKMHKRE RTCKDYIKAV VETVDNLLRP EALESWKDMN ATEQAHTATM 650
    LLDVLEEGAF LLADNVREPA RFLAAKQNVV LEVTVLNTEG QVQELVFPQE 700
    YPSENSIQLS ANTIKQNSRN GVVKVVFILY NNLGLFLSTE NATVKLAGEA 750
    GSGGPGGASL VVNSQVIAAS INKESSRVFL MDPVIFTVAH LEAKNHFNAN 800
    CSFWNYSERS MLGYWSTQGC RLVESNKTHT TCACSHLTNF AVLMAHREIY 850
    QGRINELLLS VITWVGIVIS LVCLAICIST FCFLRGLQTD RNTIHKNLCI 900
    NLFLAELLFL VGIDKTQYEI ACPIFAGLLH YFFLAAFSWL CLEGVHLYLL 950
    LVEVFESEYS RTKYYYLGGY CFPALVVGIA AAIDYRSYGT EKACWLRVDN 1000
    YFIWSFIGPV SFVIVVNLVF LMVTLHKMVR SSSVLKPDSS RLDNIKSWAL 1050
    GAIALLFLLG LTWAFGLLFI NKESVVMAYL FTTFNAFQGV FIFVFHCALQ 1100
    KKVHKEYSKC LRHSYCCIRS PPGGAHGSLK TSAMRSNARY YTGTQSRIRR 1150
    MWNDTVRKQT ESSFMAGDIN STPTLNRGTM GNHLLTNPVL QPRGGTSPYN 1200
    TLIAESVGFN PSSPPVFNSP GSYREPKHPL GGREACGMDT LPLNGNFNNS 1250
    YSLRSGDFPP GDGAPEPPRG RNLADAAAFE KMIISELVHN NLRGGSSGAK 1300
    GPPPPEPPVP PVPGGSGEEE AGGPGADRAE IELLYKALEE PLLLPRAQSV 1350
    LYQSDLDESE SCTAEDGATS RPLSSPPGRD SLYASGANLR DSPSYPDSSP 1400
    EGPSEALPPP PPAPPGPPEI YYTSRPPALV ARNPLQGYYQ VRRPSHEGYL 1450
    AAPGLEGPGP DGDGQMQLVT SL 1472
    Length:1,472
    Mass (Da):162,205
    Last modified:May 1, 1999 - v1
    Checksum:i860D7C75AA0D2F24
    GO
    Isoform 2 (identifier: O97831-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         132-137: KVKQKV → I

    Show »
    Length:1,467
    Mass (Da):161,608
    Checksum:i0F8079CF9E8FC6DD
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei132 – 1376KVKQKV → I in isoform 2. 2 PublicationsVSP_022136

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF111097 mRNA. Translation: AAD09191.1.
    AF111098 mRNA. Translation: AAD09192.1.
    PIRiT18411.
    T18413.
    RefSeqiNP_001107200.1. NM_001113728.1. [O97831-1]
    XP_005208683.1. XM_005208626.1. [O97831-2]
    UniGeneiBt.27855.

    Genome annotation databases

    EnsembliENSBTAT00000004789; ENSBTAP00000004789; ENSBTAG00000003675. [O97831-2]
    GeneIDi788252.
    KEGGibta:788252.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF111097 mRNA. Translation: AAD09191.1 .
    AF111098 mRNA. Translation: AAD09192.1 .
    PIRi T18411.
    T18413.
    RefSeqi NP_001107200.1. NM_001113728.1. [O97831-1 ]
    XP_005208683.1. XM_005208626.1. [O97831-2 ]
    UniGenei Bt.27855.

    3D structure databases

    ProteinModelPortali O97831.
    SMRi O97831. Positions 29-134.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S63.013.
    GPCRDBi Search...

    Proteomic databases

    PRIDEi O97831.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000004789 ; ENSBTAP00000004789 ; ENSBTAG00000003675 . [O97831-2 ]
    GeneIDi 788252.
    KEGGi bta:788252.

    Organism-specific databases

    CTDi 22859.

    Phylogenomic databases

    eggNOGi NOG253931.
    GeneTreei ENSGT00710000106375.
    HOGENOMi HOG000049065.
    HOVERGENi HBG052337.
    InParanoidi O97831.
    KOi K04592.

    Miscellaneous databases

    NextBioi 20928986.

    Family and domain databases

    InterProi IPR022624. DUF3497.
    IPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR003924. GPCR_2_latrophilin.
    IPR003334. GPCR_2_latrophilin_rcpt_C.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    IPR000203. GPS.
    IPR000922. Lectin_gal-bd_dom.
    IPR003112. Olfac-like.
    [Graphical view ]
    Pfami PF00002. 7tm_2. 1 hit.
    PF12003. DUF3497. 1 hit.
    PF02140. Gal_Lectin. 1 hit.
    PF01825. GPS. 1 hit.
    PF02793. HRM. 1 hit.
    PF02354. Latrophilin. 1 hit.
    PF02191. OLF. 1 hit.
    [Graphical view ]
    PRINTSi PR00249. GPCRSECRETIN.
    PR01444. LATROPHILIN.
    SMARTi SM00303. GPS. 1 hit.
    SM00008. HormR. 1 hit.
    SM00284. OLF. 1 hit.
    [Graphical view ]
    PROSITEi PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    PS50221. GPS. 1 hit.
    PS51132. OLF. 1 hit.
    PS50228. SUEL_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alpha-latrotoxin receptor, latrophilin, is a novel member of the secretin family of G protein-coupled receptors."
      Lelianova V.G., Davletov B.A., Sterling A., Rahman M.A., Grishin E.V., Totty N.F., Ushkaryov Y.A.
      J. Biol. Chem. 272:21504-21508(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain.
    2. "The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution."
      Matsushita H., Lelianova V.G., Ushkaryov Y.A.
      FEBS Lett. 443:348-352(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
      Tissue: Brain.

    Entry informationi

    Entry nameiLPHN1_BOVIN
    AccessioniPrimary (citable) accession number: O97831
    Secondary accession number(s): O97830
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2004
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3