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O97831 (LPHN1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Latrophilin-1
Alternative name(s):
Calcium-independent alpha-latrotoxin receptor 1
Short name=CIRL-1
Gene names
Name:LPHN1
Synonyms:LPH1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length1472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization. Receptor propably implicated in the regulation of exocytosis By similarity.

Subunit structure

Forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). Interacts with syntaxin and with protein of the SHANK family via the PDZ domain By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell projectionaxon. Cell projectiongrowth cone. Cell junctionsynapse. Cell junctionsynapsepresynaptic cell membrane. Cell junctionsynapsesynaptosome. Note: Colocalizes with TENM2 on the cell surface, across intercellular junctions and on nerve terminals near synaptic clefts.

Tissue specificity

Brain-specific expression but low levels are also detected in kidney, lung and spleen. Ref.2

Domain

The extracellular domain coupled to the a single transmembrane region are sufficient for full responsiveness to alpha-latrotoxin By similarity.

Post-translational modification

Autoproteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. This proteolytic processing takes place early in the biosynthetic pathway, either in the endoplasmic reticulum or in the early compartment of the Golgi apparatus By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Contains 1 GPS domain.

Contains 1 olfactomedin-like domain.

Contains 1 SUEL-type lectin domain.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Cell projection
Membrane
Synapse
Synaptosome
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   LigandLectin
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcalcium-mediated signaling using intracellular calcium source

Inferred from sequence or structural similarity. Source: UniProtKB

heterophilic cell-cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

neuropeptide signaling pathway

Inferred from electronic annotation. Source: InterPro

positive regulation of synapse maturation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentaxon

Inferred from sequence or structural similarity. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

growth cone

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

presynaptic membrane

Inferred from sequence or structural similarity. Source: UniProtKB

synapse

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

cell adhesion molecule binding

Inferred from sequence or structural similarity. Source: UniProtKB

latrotoxin receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O97831-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O97831-2)

The sequence of this isoform differs from the canonical sequence as follows:
     132-137: KVKQKV → I

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 By similarity
Chain25 – 14721448Latrophilin-1
PRO_0000012906

Regions

Topological domain25 – 857833Extracellular Potential
Transmembrane858 – 87821Helical; Name=1; Potential
Topological domain879 – 89214Cytoplasmic Potential
Transmembrane893 – 91321Helical; Name=2; Potential
Topological domain914 – 9196Extracellular Potential
Transmembrane920 – 94021Helical; Name=3; Potential
Topological domain941 – 96323Cytoplasmic Potential
Transmembrane964 – 98421Helical; Name=4; Potential
Topological domain985 – 100117Extracellular Potential
Transmembrane1002 – 102221Helical; Name=5; Potential
Topological domain1023 – 104927Cytoplasmic Potential
Transmembrane1050 – 107021Helical; Name=6; Potential
Topological domain1071 – 10744Extracellular Potential
Transmembrane1075 – 109521Helical; Name=7; Potential
Topological domain1096 – 1472377Cytoplasmic Potential
Domain40 – 12990SUEL-type lectin
Domain139 – 398260Olfactomedin-like
Domain798 – 84952GPS
Region117 – 1204Carbohydrate binding By similarity
Compositional bias413 – 4164Poly-Thr
Compositional bias1302 – 131312Poly-Pro
Compositional bias1408 – 141811Poly-Pro

Sites

Binding site421Carbohydrate By similarity
Site837 – 8382Cleavage; by autocatalysis By similarity

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation5311N-linked (GlcNAc...) Potential
Glycosylation6401N-linked (GlcNAc...) Potential
Glycosylation7411N-linked (GlcNAc...) Potential
Glycosylation8001N-linked (GlcNAc...) Potential
Glycosylation8051N-linked (GlcNAc...) Potential
Glycosylation8261N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 71 By similarity
Disulfide bond50 ↔ 128 By similarity
Disulfide bond83 ↔ 115 By similarity
Disulfide bond96 ↔ 102 By similarity
Disulfide bond140 ↔ 322 By similarity
Disulfide bond480 ↔ 515 By similarity
Disulfide bond503 ↔ 532 By similarity
Disulfide bond801 ↔ 832 By similarity
Disulfide bond820 ↔ 834 By similarity

Natural variations

Alternative sequence132 – 1376KVKQKV → I in isoform 2.
VSP_022136

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 860D7C75AA0D2F24

FASTA1,472162,205
        10         20         30         40         50         60 
MARLAAVLWS LCVTAILVTS ATQGLSRAGL PFGLMRRELA CEGYPIELRC PGSDVIMVEN 

        70         80         90        100        110        120 
ANYGRTDDKI CDADPFQMEN VQCYLPDAFK IMSQRCNNRT QCVVVAGSDA FPDPCPGTYK 

       130        140        150        160        170        180 
YLEVQYDCVP YKVKQKVFVC PGTLQKVLEP TSTHESEHQS GAWCKDPLQA GDRIYVMPWI 

       190        200        210        220        230        240 
PYRTDTLTEY ASWEDYVAAR HTTTYRLPNR VDGTGFVVYD GAVFYNKERT RNIVKYDLRT 

       250        260        270        280        290        300 
RIKSGETVIN TANYHDTSPY RWGGKTDIDL AVDENGLWVI YATEGNNGRL VVSQLNPYTL 

       310        320        330        340        350        360 
RFEGTWETGY DKRSASNAFM VCGVLYVLRS VYVDDDSEAA GNRVDYAFNT NANREEPVSL 

       370        380        390        400        410        420 
AFPNPYQFVS SVDYNPRDNQ LYVWNNYFVV RYSLEFGPPD PSAGPATSPP LSTTTTARPT 

       430        440        450        460        470        480 
PLTSTASPAA TTPLRRAPLT THPVGAINQL GPDLPPATAP APSTRRPPAP NLHVSPELFC 

       490        500        510        520        530        540 
EPREVRRVQW PATQQGMLVE RPCPKGTRGI ASFQCLPALG LWNPRGPDLS NCTSPWVNQV 

       550        560        570        580        590        600 
AQKIKSGENA ANIASELARH TRGSIYAGDV SSSVKLMEQL LDILDAQLQA LRPIERESAG 

       610        620        630        640        650        660 
KNYNKMHKRE RTCKDYIKAV VETVDNLLRP EALESWKDMN ATEQAHTATM LLDVLEEGAF 

       670        680        690        700        710        720 
LLADNVREPA RFLAAKQNVV LEVTVLNTEG QVQELVFPQE YPSENSIQLS ANTIKQNSRN 

       730        740        750        760        770        780 
GVVKVVFILY NNLGLFLSTE NATVKLAGEA GSGGPGGASL VVNSQVIAAS INKESSRVFL 

       790        800        810        820        830        840 
MDPVIFTVAH LEAKNHFNAN CSFWNYSERS MLGYWSTQGC RLVESNKTHT TCACSHLTNF 

       850        860        870        880        890        900 
AVLMAHREIY QGRINELLLS VITWVGIVIS LVCLAICIST FCFLRGLQTD RNTIHKNLCI 

       910        920        930        940        950        960 
NLFLAELLFL VGIDKTQYEI ACPIFAGLLH YFFLAAFSWL CLEGVHLYLL LVEVFESEYS 

       970        980        990       1000       1010       1020 
RTKYYYLGGY CFPALVVGIA AAIDYRSYGT EKACWLRVDN YFIWSFIGPV SFVIVVNLVF 

      1030       1040       1050       1060       1070       1080 
LMVTLHKMVR SSSVLKPDSS RLDNIKSWAL GAIALLFLLG LTWAFGLLFI NKESVVMAYL 

      1090       1100       1110       1120       1130       1140 
FTTFNAFQGV FIFVFHCALQ KKVHKEYSKC LRHSYCCIRS PPGGAHGSLK TSAMRSNARY 

      1150       1160       1170       1180       1190       1200 
YTGTQSRIRR MWNDTVRKQT ESSFMAGDIN STPTLNRGTM GNHLLTNPVL QPRGGTSPYN 

      1210       1220       1230       1240       1250       1260 
TLIAESVGFN PSSPPVFNSP GSYREPKHPL GGREACGMDT LPLNGNFNNS YSLRSGDFPP 

      1270       1280       1290       1300       1310       1320 
GDGAPEPPRG RNLADAAAFE KMIISELVHN NLRGGSSGAK GPPPPEPPVP PVPGGSGEEE 

      1330       1340       1350       1360       1370       1380 
AGGPGADRAE IELLYKALEE PLLLPRAQSV LYQSDLDESE SCTAEDGATS RPLSSPPGRD 

      1390       1400       1410       1420       1430       1440 
SLYASGANLR DSPSYPDSSP EGPSEALPPP PPAPPGPPEI YYTSRPPALV ARNPLQGYYQ 

      1450       1460       1470 
VRRPSHEGYL AAPGLEGPGP DGDGQMQLVT SL 

« Hide

Isoform 2 [UniParc].

Checksum: 0F8079CF9E8FC6DD
Show »

FASTA1,467161,608

References

[1]"Alpha-latrotoxin receptor, latrophilin, is a novel member of the secretin family of G protein-coupled receptors."
Lelianova V.G., Davletov B.A., Sterling A., Rahman M.A., Grishin E.V., Totty N.F., Ushkaryov Y.A.
J. Biol. Chem. 272:21504-21508(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[2]"The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution."
Matsushita H., Lelianova V.G., Ushkaryov Y.A.
FEBS Lett. 443:348-352(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF111097 mRNA. Translation: AAD09191.1.
AF111098 mRNA. Translation: AAD09192.1.
PIRT18411.
T18413.
RefSeqNP_001107200.1. NM_001113728.1. [O97831-1]
XP_005208683.1. XM_005208626.1. [O97831-2]
UniGeneBt.27855.

3D structure databases

ProteinModelPortalO97831.
SMRO97831. Positions 29-134.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS63.013.
GPCRDBSearch...

Proteomic databases

PRIDEO97831.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000004789; ENSBTAP00000004789; ENSBTAG00000003675. [O97831-2]
GeneID788252.
KEGGbta:788252.

Organism-specific databases

CTD22859.

Phylogenomic databases

eggNOGNOG253931.
GeneTreeENSGT00710000106375.
HOGENOMHOG000049065.
HOVERGENHBG052337.
InParanoidO97831.
KOK04592.

Family and domain databases

InterProIPR022624. DUF3497.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF12003. DUF3497. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
PRINTSPR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
[Graphical view]
PROSITEPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20928986.

Entry information

Entry nameLPHN1_BOVIN
AccessionPrimary (citable) accession number: O97831
Secondary accession number(s): O97830
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries