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O97831

- LPHN1_BOVIN

UniProt

O97831 - LPHN1_BOVIN

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Protein

Latrophilin-1

Gene

LPHN1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization. Receptor propably implicated in the regulation of exocytosis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421CarbohydrateBy similarity
Sitei837 – 8382Cleavage; by autocatalysisBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. cell adhesion molecule binding Source: UniProtKB
  3. G-protein coupled receptor activity Source: UniProtKB-KW
  4. latrotoxin receptor activity Source: UniProtKB

GO - Biological processi

  1. calcium-mediated signaling using intracellular calcium source Source: UniProtKB
  2. heterophilic cell-cell adhesion Source: UniProtKB
  3. neuropeptide signaling pathway Source: InterPro
  4. positive regulation of synapse maturation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Lectin

Protein family/group databases

MEROPSiS63.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Latrophilin-1
Alternative name(s):
Calcium-independent alpha-latrotoxin receptor 1
Short name:
CIRL-1
Gene namesi
Name:LPHN1
Synonyms:LPH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 7

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cell projectionaxon. Cell projectiongrowth cone. Cell junctionsynapse. Cell junctionsynapsepresynaptic cell membrane. Cell junctionsynapsesynaptosome
Note: Colocalizes with TENM2 on the cell surface, across intercellular junctions and on nerve terminals near synaptic clefts.

GO - Cellular componenti

  1. axon Source: UniProtKB
  2. cell junction Source: UniProtKB-KW
  3. growth cone Source: UniProtKB
  4. integral component of membrane Source: UniProtKB-KW
  5. neuron projection Source: UniProtKB
  6. plasma membrane Source: UniProtKB
  7. presynaptic membrane Source: UniProtKB
  8. synapse Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424By similarityAdd
BLAST
Chaini25 – 14721448Latrophilin-1PRO_0000012906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 71PROSITE-ProRule annotation
Disulfide bondi50 ↔ 128PROSITE-ProRule annotation
Disulfide bondi83 ↔ 115PROSITE-ProRule annotation
Disulfide bondi96 ↔ 102PROSITE-ProRule annotation
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi140 ↔ 322PROSITE-ProRule annotation
Disulfide bondi480 ↔ 515PROSITE-ProRule annotation
Disulfide bondi503 ↔ 532PROSITE-ProRule annotation
Glycosylationi531 – 5311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi640 – 6401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi741 – 7411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi800 – 8001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi801 ↔ 832PROSITE-ProRule annotation
Glycosylationi805 – 8051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi820 ↔ 834PROSITE-ProRule annotation
Glycosylationi826 – 8261N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Autoproteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. This proteolytic processing takes place early in the biosynthetic pathway, either in the endoplasmic reticulum or in the early compartment of the Golgi apparatus (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO97831.

Expressioni

Tissue specificityi

Brain-specific expression but low levels are also detected in kidney, lung and spleen.1 Publication

Gene expression databases

ExpressionAtlasiO97831. baseline.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). Interacts with syntaxin and with protein of the SHANK family via the PDZ domain (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO97831.
SMRiO97831. Positions 29-134.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 857833ExtracellularSequence AnalysisAdd
BLAST
Topological domaini879 – 89214CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini914 – 9196ExtracellularSequence Analysis
Topological domaini941 – 96323CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini985 – 100117ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1023 – 104927CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini1071 – 10744ExtracellularSequence Analysis
Topological domaini1096 – 1472377CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei858 – 87821Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei893 – 91321Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei920 – 94021Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei964 – 98421Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei1002 – 102221Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei1050 – 107021Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei1075 – 109521Helical; Name=7Sequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 12990SUEL-type lectinPROSITE-ProRule annotationAdd
BLAST
Domaini139 – 398260Olfactomedin-likePROSITE-ProRule annotationAdd
BLAST
Domaini798 – 84952GPSPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 1204Carbohydrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi413 – 4164Poly-Thr
Compositional biasi1302 – 131312Poly-ProAdd
BLAST
Compositional biasi1408 – 141811Poly-ProAdd
BLAST

Domaini

The extracellular domain coupled to the a single transmembrane region are sufficient for full responsiveness to alpha-latrotoxin.By similarity

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation
Contains 1 olfactomedin-like domain.PROSITE-ProRule annotation
Contains 1 SUEL-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253931.
GeneTreeiENSGT00760000118787.
HOGENOMiHOG000049065.
HOVERGENiHBG052337.
InParanoidiO97831.
KOiK04592.

Family and domain databases

InterProiIPR022624. DUF3497.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF12003. DUF3497. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTiSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
[Graphical view]
PROSITEiPS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O97831-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARLAAVLWS LCVTAILVTS ATQGLSRAGL PFGLMRRELA CEGYPIELRC
60 70 80 90 100
PGSDVIMVEN ANYGRTDDKI CDADPFQMEN VQCYLPDAFK IMSQRCNNRT
110 120 130 140 150
QCVVVAGSDA FPDPCPGTYK YLEVQYDCVP YKVKQKVFVC PGTLQKVLEP
160 170 180 190 200
TSTHESEHQS GAWCKDPLQA GDRIYVMPWI PYRTDTLTEY ASWEDYVAAR
210 220 230 240 250
HTTTYRLPNR VDGTGFVVYD GAVFYNKERT RNIVKYDLRT RIKSGETVIN
260 270 280 290 300
TANYHDTSPY RWGGKTDIDL AVDENGLWVI YATEGNNGRL VVSQLNPYTL
310 320 330 340 350
RFEGTWETGY DKRSASNAFM VCGVLYVLRS VYVDDDSEAA GNRVDYAFNT
360 370 380 390 400
NANREEPVSL AFPNPYQFVS SVDYNPRDNQ LYVWNNYFVV RYSLEFGPPD
410 420 430 440 450
PSAGPATSPP LSTTTTARPT PLTSTASPAA TTPLRRAPLT THPVGAINQL
460 470 480 490 500
GPDLPPATAP APSTRRPPAP NLHVSPELFC EPREVRRVQW PATQQGMLVE
510 520 530 540 550
RPCPKGTRGI ASFQCLPALG LWNPRGPDLS NCTSPWVNQV AQKIKSGENA
560 570 580 590 600
ANIASELARH TRGSIYAGDV SSSVKLMEQL LDILDAQLQA LRPIERESAG
610 620 630 640 650
KNYNKMHKRE RTCKDYIKAV VETVDNLLRP EALESWKDMN ATEQAHTATM
660 670 680 690 700
LLDVLEEGAF LLADNVREPA RFLAAKQNVV LEVTVLNTEG QVQELVFPQE
710 720 730 740 750
YPSENSIQLS ANTIKQNSRN GVVKVVFILY NNLGLFLSTE NATVKLAGEA
760 770 780 790 800
GSGGPGGASL VVNSQVIAAS INKESSRVFL MDPVIFTVAH LEAKNHFNAN
810 820 830 840 850
CSFWNYSERS MLGYWSTQGC RLVESNKTHT TCACSHLTNF AVLMAHREIY
860 870 880 890 900
QGRINELLLS VITWVGIVIS LVCLAICIST FCFLRGLQTD RNTIHKNLCI
910 920 930 940 950
NLFLAELLFL VGIDKTQYEI ACPIFAGLLH YFFLAAFSWL CLEGVHLYLL
960 970 980 990 1000
LVEVFESEYS RTKYYYLGGY CFPALVVGIA AAIDYRSYGT EKACWLRVDN
1010 1020 1030 1040 1050
YFIWSFIGPV SFVIVVNLVF LMVTLHKMVR SSSVLKPDSS RLDNIKSWAL
1060 1070 1080 1090 1100
GAIALLFLLG LTWAFGLLFI NKESVVMAYL FTTFNAFQGV FIFVFHCALQ
1110 1120 1130 1140 1150
KKVHKEYSKC LRHSYCCIRS PPGGAHGSLK TSAMRSNARY YTGTQSRIRR
1160 1170 1180 1190 1200
MWNDTVRKQT ESSFMAGDIN STPTLNRGTM GNHLLTNPVL QPRGGTSPYN
1210 1220 1230 1240 1250
TLIAESVGFN PSSPPVFNSP GSYREPKHPL GGREACGMDT LPLNGNFNNS
1260 1270 1280 1290 1300
YSLRSGDFPP GDGAPEPPRG RNLADAAAFE KMIISELVHN NLRGGSSGAK
1310 1320 1330 1340 1350
GPPPPEPPVP PVPGGSGEEE AGGPGADRAE IELLYKALEE PLLLPRAQSV
1360 1370 1380 1390 1400
LYQSDLDESE SCTAEDGATS RPLSSPPGRD SLYASGANLR DSPSYPDSSP
1410 1420 1430 1440 1450
EGPSEALPPP PPAPPGPPEI YYTSRPPALV ARNPLQGYYQ VRRPSHEGYL
1460 1470
AAPGLEGPGP DGDGQMQLVT SL
Length:1,472
Mass (Da):162,205
Last modified:May 1, 1999 - v1
Checksum:i860D7C75AA0D2F24
GO
Isoform 2 (identifier: O97831-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-137: KVKQKV → I

Show »
Length:1,467
Mass (Da):161,608
Checksum:i0F8079CF9E8FC6DD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei132 – 1376KVKQKV → I in isoform 2. 2 PublicationsVSP_022136

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF111097 mRNA. Translation: AAD09191.1.
AF111098 mRNA. Translation: AAD09192.1.
PIRiT18411.
T18413.
RefSeqiNP_001107200.1. NM_001113728.1. [O97831-1]
XP_005208683.1. XM_005208626.1. [O97831-2]
UniGeneiBt.27855.

Genome annotation databases

EnsembliENSBTAT00000004789; ENSBTAP00000004789; ENSBTAG00000003675. [O97831-2]
GeneIDi788252.
KEGGibta:788252.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF111097 mRNA. Translation: AAD09191.1 .
AF111098 mRNA. Translation: AAD09192.1 .
PIRi T18411.
T18413.
RefSeqi NP_001107200.1. NM_001113728.1. [O97831-1 ]
XP_005208683.1. XM_005208626.1. [O97831-2 ]
UniGenei Bt.27855.

3D structure databases

ProteinModelPortali O97831.
SMRi O97831. Positions 29-134.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S63.013.
GPCRDBi Search...

Proteomic databases

PRIDEi O97831.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000004789 ; ENSBTAP00000004789 ; ENSBTAG00000003675 . [O97831-2 ]
GeneIDi 788252.
KEGGi bta:788252.

Organism-specific databases

CTDi 22859.

Phylogenomic databases

eggNOGi NOG253931.
GeneTreei ENSGT00760000118787.
HOGENOMi HOG000049065.
HOVERGENi HBG052337.
InParanoidi O97831.
KOi K04592.

Miscellaneous databases

NextBioi 20928986.

Gene expression databases

ExpressionAtlasi O97831. baseline.

Family and domain databases

InterProi IPR022624. DUF3497.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like.
[Graphical view ]
Pfami PF00002. 7tm_2. 1 hit.
PF12003. DUF3497. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view ]
PRINTSi PR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTi SM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
[Graphical view ]
PROSITEi PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Alpha-latrotoxin receptor, latrophilin, is a novel member of the secretin family of G protein-coupled receptors."
    Lelianova V.G., Davletov B.A., Sterling A., Rahman M.A., Grishin E.V., Totty N.F., Ushkaryov Y.A.
    J. Biol. Chem. 272:21504-21508(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  2. "The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution."
    Matsushita H., Lelianova V.G., Ushkaryov Y.A.
    FEBS Lett. 443:348-352(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiLPHN1_BOVIN
AccessioniPrimary (citable) accession number: O97831
Secondary accession number(s): O97830
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3