Reviewed, UniProtKB/Swiss-Prot O97756 (SNAT_MACMU)

Names and origin

Protein names

Recommended name:
    Serotonin N-acetyltransferase
      Short name=Serotonin acetylase
    EC=2.3.1.87
Alternative name(s):
    Aralkylamine N-acetyltransferase
      Short name=AA-NAT

Gene names

Name:	AANAT
Synonyms:	SNAT

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the N-acetylation of serotonin into N-acetylserotonin.
Catalytic activity	Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine.
Pathway	Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
Subunit structure	Monomer By similarity. Interacts with YWHAZ; the interaction requires phosphorylation on Thr-28, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-202 site, the other YWHAZ monomer with similar effect By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highly expressed in pineal gland and retina.
Post-translational modification	Phosphorylated; cAMP-dependent phosphorylation on both N-terminal and C-terminal sites regulates AANAT activity through allowing interaction with 14-3-3 proteins and protecting the enzyme against proteasomal degradation By similarity.
Sequence similarities	Belongs to the acetyltransferase family. AANAT subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Biological rhythms Melatonin biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	melatonin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aralkylamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Serotonin N-acetyltransferase		PRO_0000074581
Regions
	Domain	32 – 193	162	N-acetyltransferase
	Region	121 – 123	3	Acetyl-CoA binding By similarity
	Region	129 – 134	6	Acetyl-CoA binding By similarity
	Region	165 – 167	3	Acetyl-CoA binding By similarity
Sites
	Binding site	121	1	Substrate; via carbonyl oxygen By similarity
	Site	118	1	Important for the catalytic mechanism; involved in substrate deprotonation By similarity
	Site	120	1	Important for the catalytic mechanism; involved in substrate deprotonation By similarity
Amino acid modifications
	Modified residue	28	1	Phosphothreonine; by PKA By similarity
	Modified residue	202	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O97756-1 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 40A5A415E554DF79	FASTA	204	22,891
	10 20 30 40 50 60 MSTQSTHPPK PEAPRLPPAI SSCQRRHTLP ASEFRCLTPE DAVSAFEIER EAFISVLGVC 70 80 90 100 110 120 PLYLDEIRHF LTLCPELSLG WFEEGCLVAF IIGSLWDKDR LMQESLTMHR PGGHIAHLHV 130 140 150 160 170 180 LAVHCAFRQQ GRGPILLWRY LHHLGSQPAV HRAALMCEDA LVPFYERFGF HAMGPCAITV 190 200 GSLSFTELHC SLQGHPFLRR NSGC

References

[1]	Coon S.L. Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pineal gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U46661 mRNA. Translation: AAD00061.2.
RefSeq	NP_001040592.1.
UniGene	Mmu.3603
3D structure databases
SMR	O97756. Positions 18-193.
ModBase	Search...
Protein-protein interaction databases
STRING	O97756.
Genome annotation databases
Ensembl	ENSMMUT00000026668; ENSMMUP00000024958; ENSMMUG00000018970; Macaca mulatta. [Genome view] ENSMMUT00000040995; ENSMMUP00000034025; ENSMMUG00000018970; Macaca mulatta. [Genome view]
GeneID	706924.
KEGG	mcc:706924.
Organism-specific databases
CTD	706924.
Phylogenomic databases
eggNOG	prNOG15095.
HOVERGEN	O97756.
InParanoid	O97756.
OMA	VPFYERF.
OrthoDB	EOG9GF60Z.
PhylomeDB	O97756.
Enzyme and pathway databases
BRENDA	2.3.1.87. 1774.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SNAT_MACMU

Accession

Primary (citable) accession number: O97756

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 27, 2002
Last modified:	January 19, 2010
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)