Reviewed, UniProtKB/Swiss-Prot O97711 (HAS2_BOVIN)

Names and origin

Protein names

Recommended name:
    Hyaluronan synthase 2
    EC=2.4.1.212
Alternative name(s):
    Hyaluronate synthase 2
    Hyaluronic acid synthase 2
      Short name=HA synthase 2

Gene names

Name:

HAS2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis By similarity.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed in corneal endothelial cells. Ref.3
Sequence similarities	Belongs to the nodC/HAS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Hyaluronan synthase 2		PRO_0000197172
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	1 Potential
	Topological domain	33 – 45	13	Extracellular Potential
	Transmembrane	46 – 66	21	2 Potential
	Topological domain	67 – 374	308	Cytoplasmic Potential
	Transmembrane	375 – 395	21	3 Potential
	Topological domain	396 – 402	7	Extracellular Potential
	Transmembrane	403 – 423	21	4 Potential
	Topological domain	424 – 429	6	Cytoplasmic Potential
	Transmembrane	430 – 450	21	5 Potential
	Topological domain	451 – 475	25	Extracellular Potential
	Transmembrane	476 – 496	21	6 Potential
	Topological domain	497 – 510	14	Cytoplasmic Potential
	Transmembrane	511 – 531	21	7 Potential
	Topological domain	532 – 552	21	Extracellular Potential
Experimental info
	Sequence conflict	406	1	T → I in BAA76547. Ref.2
	Sequence conflict	425	1	S → C in BAA76547. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O97711-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 85AD256435A5EB64	FASTA	552	63,459
	10 20 30 40 50 60 MHCERFLCIL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG AFLASHLIIQ 70 80 90 100 110 120 SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR KCLQSVKRLT YPGIKVVMVI 130 140 150 160 170 180 DGNSEDDLYM MDIFSEVMGR DKSATYIWKN NYHVKGPGET DESHKESSQH VTQLVLSNKS 190 200 210 220 230 240 ICTMQKWGGK REVMYTAFRA LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG 250 260 270 280 290 300 DVQILNKYDS WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY 310 320 330 340 350 360 NQEFMGSQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ QTRWSKSYFR 370 380 390 400 410 420 EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR GKIWNTLLFL LTVQLVGLIK 430 440 450 460 470 480 SSFASCLRGN IVMVFMSLYS VLYMSSLLPA KMFAIATINK AGWGTSGRKT IVVNFIGLIP 490 500 510 520 530 540 VSVWFTILLG GVIFTIYKES KKPFSESKQT VLIVGTLLYA CYWVMLLTLY VVLINKCGRR 550 KKGQQYDMVL DV

References

[1]	"Hyaluronan synthase is expressed during cumulus-oocyte complex maturation in the cow." Einspanier R., Patzner K., Lauer B., Berisha B. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"HAS2." Yamashita H., Usui T., Suzuki K. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 267-469.
[3]	"Hyaluronan synthase expression in bovine eyes." Usui T., Suzuki K., Kaji Y., Amano S., Miyata K., Heldin P., Yamashita H. Invest. Ophthalmol. Vis. Sci. 40:563-567(1999) [PubMed: 10067958] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ004951 mRNA. Translation: CAA06239.1. AB017804 mRNA. Translation: BAA76547.1.
IPI	IPI00709204.
RefSeq	NP_776504.1.
UniGene	Bt.63577
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
Ensembl	ENSBTAT00000026503; ENSBTAP00000026503; ENSBTAG00000019892; Bos taurus. [Genome view]
GeneID	281220.
KEGG	bta:281220.
Organism-specific databases
CTD	281220.
Phylogenomic databases
HOVERGEN	O97711.
Enzyme and pathway databases
BRENDA	2.4.1.212. 251.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HAS2_BOVIN

Accession

Primary (citable) accession number: O97711

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	October 13, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)