Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sterol regulatory element-binding protein 1

Gene

SREBF1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol regulatory element-binding protein 1
Short name:
SREBP-1
Alternative name(s):
Adipocyte determination and differentiation-dependent factor 1
Sterol regulatory element-binding transcription factor 1
Cleaved into the following chain:
Gene namesi
Name:SREBF1
Synonyms:ADD1, SREBP1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Processed sterol regulatory element-binding protein 1 :
  • Nucleus By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 487487CytoplasmicSequence analysisAdd
BLAST
Transmembranei488 – 50821HelicalSequence analysisAdd
BLAST
Topological domaini509 – 54739LumenalSequence analysisAdd
BLAST
Transmembranei548 – 56821HelicalSequence analysisAdd
BLAST
Topological domaini569 – 1151583CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11511151Sterol regulatory element-binding protein 1PRO_0000127449Add
BLAST
Chaini1 – 490490Processed sterol regulatory element-binding protein 1PRO_0000317058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei98 – 981PhosphoserineBy similarity
Modified residuei117 – 1171PhosphoserineBy similarity
Modified residuei338 – 3381Phosphoserine; by SIK1By similarity
Modified residuei339 – 3391Phosphoserine; by SIK1By similarity
Modified residuei397 – 3971Phosphoserine; by AMPKBy similarity
Modified residuei403 – 4031Phosphoserine; by SIK1By similarity
Modified residuei457 – 4571PhosphoserineBy similarity

Post-translational modificationi

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7 (By similarity).By similarity
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-403 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei460 – 4612Cleavage; by caspase-3 and caspase-7By similarity
Sitei490 – 4912Cleavage; by S2PBy similarity
Sitei530 – 5312Cleavage; by S1PBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Interacts with CEBPA, the interaction produces a transcriptional synergy (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO97676.
SMRiO97676. Positions 320-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini324 – 37451bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5959Transcriptional activation (acidic)By similarityAdd
BLAST
Regioni234 – 497264Interaction with LMNABy similarityAdd
BLAST
Regioni374 – 39623Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 179120Pro/Ser-richAdd
BLAST
Compositional biasi427 – 46236Gly/Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the SREBP family.Curated
Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG061592.
InParanoidiO97676.
KOiK07197.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEPPFTEAA LEQALAEPCE LDAALLTDIE DMLQLINNQD SDFPGLFDAP
60 70 80 90 100
YAGVAGGTDP TSPDASSPGS PTPPPSTMSS PLEGFLGGAR TPPPPPVSPT
110 120 130 140 150
QPAPTPLKMY PSVPAFSPGP GIKEEPVPLT ILQPPTPQPL SGALLPQSLP
160 170 180 190 200
ALAPPQLSPA PVLGYPSPPG SFSSATPPGS TSQTLPGLPL ASLPGVLPVS
210 220 230 240 250
VHTQVQSAAP QQLLTATATP VVSPGTTTVT SQIQQVPVLL QPHFIKADSL
260 270 280 290 300
LLTTMKTDMG TPVKAAGIGS LAPGTAVQAA PLQTLVSGGT ILATVPLVVD
310 320 330 340 350
TDKLPINRLA AGGKALSSGQ SRGEKRTAHN AIEKRYRSSI NDKIIELKDL
360 370 380 390 400
VVGTEAKLNK SAVLRKAIDY IRFLQQSNQK LKQENLSLRT AAHKSKSLKD
410 420 430 440 450
LVSCSSGGRT DVPMEGVKPE VVDTLSPPPS DAGSPSQSSP LSLGSRGSSS
460 470 480 490 500
GGSGSDSEPD SPVFEDSQMK PEQLPAPHGR GMLDRSRLAL CVLVFLCLSC
510 520 530 540 550
NPLASLMGSW ALPGPSDATS AYHGPWRSVL GAEGRDGPGW VLWLLPPLVW
560 570 580 590 600
LTNGLLVLLF LALLFVYGEP VTRPHSDPAV RFWRHRKQAD LDLARGDFAQ
610 620 630 640 650
AAQQLWLALR ALGRPLPTSH LDLACSLLWN LIRHLLQRLW VGRWLAGRAG
660 670 680 690 700
GLRRDSALEA DTRTSACDAA LVYHKLHQLH TMGKYPGGHL DAANLALSAL
710 720 730 740 750
NLAECAGDAL SVAVLAEVYV AAALRVKTSL PRALHFLTRF FLSSARQACL
760 770 780 790 800
AQSGSVPVAM QWLCHPVGHR FFVDGDWAVC GAPRDSLYSV AGNPVDPLAQ
810 820 830 840 850
VTQLFREHLL EQALHCVAQP SPGPGSADGD REFSEALGFL QLLNSCCDTA
860 870 880 890 900
GAPACSFSIA SSTAATAGTD PVAKWWASLT AVVTHWLGRD EEAAERLYPL
910 920 930 940 950
VEHLPRALQE SERPLPRAAL HSFKAARALL GRGKAESGSA SLAMCEKASG
960 970 980 990 1000
YLQDSLAATP AGSSIDKAMQ LLLCDLLLVA RTSLWQRQKP PPPSQASQGS
1010 1020 1030 1040 1050
SSGAQASALE LRGFQRDLSG LRRLAQSFRP AMRRVFLHEA TARLMAGASP
1060 1070 1080 1090 1100
ARTHQLLDRS LRRRAGPCGR GGAAAAAAAE LEPRPTRREQ AEALLLASCY
1110 1120 1130 1140 1150
LPPGFLSAPG QRVGMLAEAA RTLEKLGDRR LLHDCQQMLM RLGGGTTVTS

S
Length:1,151
Mass (Da):121,502
Last modified:February 5, 2008 - v2
Checksum:iF9AEA7FCAF831F2D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971L → P in AAP74567 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496867 mRNA. Translation: AAS18238.1.
AY307771 mRNA. Translation: AAP74567.1.
AF102873 mRNA. Translation: AAC78685.1.
DQ464433 Genomic DNA. Translation: ABE02287.1.
RefSeqiNP_999322.1. NM_214157.1.
UniGeneiSsc.15992.

Genome annotation databases

GeneIDi397308.
KEGGissc:397308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496867 mRNA. Translation: AAS18238.1.
AY307771 mRNA. Translation: AAP74567.1.
AF102873 mRNA. Translation: AAC78685.1.
DQ464433 Genomic DNA. Translation: ABE02287.1.
RefSeqiNP_999322.1. NM_214157.1.
UniGeneiSsc.15992.

3D structure databases

ProteinModelPortaliO97676.
SMRiO97676. Positions 320-401.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397308.
KEGGissc:397308.

Organism-specific databases

CTDi6720.

Phylogenomic databases

HOVERGENiHBG061592.
InParanoidiO97676.
KOiK07197.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Pig ADD1 gene cloning and expression."
    Li C., Meng H., Zhao W., Pan Y.
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "SREBP-1c mRNA levels in the pig uterus."
    Palin M.F., Beaudry D., Murphy B.D.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 159-1151.
    Tissue: Uterus.
  3. "Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo."
    Ding S.T., McNeel R.L., Mersmann H.J.
    Comp. Biochem. Physiol. 123B:307-318(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 229-372.
  4. Chen J.F., Jiang S.W.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 230-363.

Entry informationi

Entry nameiSRBP1_PIG
AccessioniPrimary (citable) accession number: O97676
Secondary accession number(s): Q1W0S6, Q6RIB9, Q7YS02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 5, 2008
Last modified: January 20, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.