Reviewed, UniProtKB/Swiss-Prot O97627 (ARBK1_DIDMA)

Names and origin

Protein names

Recommended name:
    Beta-adrenergic receptor kinase 1
      Short name=Beta-ARK-1
    EC=2.7.11.15
Alternative name(s):
    G-protein coupled receptor kinase 2

Gene names

Name:	ADRBK1
Synonyms:	GRK2

Organism

Didelphis marsupialis virginiana (North American opossum)

Taxonomic identifier

9267 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Metatheria › Didelphimorphia › Didelphidae › Didelphis

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Does not act on HTR1B/5-hydroxytryptamine 1B receptor. Ref.1
Catalytic activity	ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.
Subunit structure	Interacts with GIT1 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: InterPro beta-adrenergic receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Beta-adrenergic receptor kinase 1		PRO_0000260315
Regions
	Domain	54 – 175	122	RGS
	Domain	191 – 453	263	Protein kinase
	Domain	454 – 521	68	AGC-kinase C-terminal
	Domain	558 – 652	95	PH
	Nucleotide binding	197 – 205	9	ATP By similarity
	Region	1 – 190	190	N-terminal
Sites
	Active site	317	1	Proton acceptor By similarity
	Binding site	220	1	ATP By similarity
Amino acid modifications
	Modified residue	670	1	Phosphoserine By similarity
Experimental info
	Mutagenesis	220	1	K → R: Loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O97627-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 8FA39ED496C7D592	FASTA	689	79,783
	10 20 30 40 50 60 MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR GEVTFEKIFS 70 80 90 100 110 120 QKLGYLLFRE FCLNHMEEAK PLVEFYDEIK KYEKLDSEEE RTVKSREIFD LYIMKELLSC 130 140 150 160 170 180 SHLFSKSATE HVQSRLLKKQ VPTDLFQPYI EEICQRFRDD VFQKFIESEK FTRFCQWKNV 190 200 210 220 230 240 ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 250 260 270 280 290 300 IMLSLVSTGD CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS ESDMRFYAAE 310 320 330 340 350 360 IILGLEHMHS RFVVYRDLKP ANILLDEFGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 370 380 390 400 410 420 VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTMA VELPDSFSPE 430 440 450 460 470 480 LRSLLEGLLQ RDVNRSLGCL GRGAQEVKED PFFKAVDWQM VLLQKYPPPL IPPRGEVNAA 490 500 510 520 530 540 DAFDIGSFDE EDTKGIKLLD SDQELYRNFP LTISERWQQE VAETVFDTVN SETDRLEARK 550 560 570 580 590 600 KAKNKQLGHE DDYALGKDCI MHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RAEGEAPQSL 610 620 630 640 650 660 LTMEEIQSVE ETQIKDRKCI LLKIRGGKQF ILQCDSDPEL VQWKKELRDV YREAQQLLQR 670 680 VPKMKNKPRS PVVELSKMPL TQRGSANGL

References

[1]

"Receptor selectivity of the cloned opossum G protein-coupled receptor kinase 2 (GRK2) in intact opossum kidney cells: role in desensitization of endogenous alpha2C-adrenergic but not serotonin 1B receptors." Lembo P.M.C., Ghahremani M.H., Albert P.R. Mol. Endocrinol. 13:138-147(1999) [PubMed: 9892019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LYS-220. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF087455 mRNA. Translation: AAD11419.1.
3D structure databases
HSSP	HSSP built from PDB template 1BAK based on UniProtKB P25098.
SMR	O97627. Positions 29-668.
ModBase	Search...
Phylogenomic databases
HOVERGEN	O97627.
Enzyme and pathway databases
BRENDA	2.7.11.15. 281319.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR015743. B-adrenergic_rcpt_kinase. IPR000239. GPCR_kinase. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR22985:SF5. BARK. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00233. PH. 1 hit. SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARBK1_DIDMA

Accession

Primary (citable) accession number: O97627

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)