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O97552 (PO5F1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name=Oct-3
Octamer-binding transcription factor 3
Short name=OTF-3
Gene names
Name:POU5F1
Synonyms:OCT3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.

Subunit structure

Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 By similarity.

Subcellular location

Cytoplasm. Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern By similarity.

Tissue specificity

Expressed in immature oocytes. Ref.1

Domain

The POU-specific domain mediates interaction with PKM By similarity.

Post-translational modification

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.

Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation By similarity.

ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription By similarity.

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360POU domain, class 5, transcription factor 1
PRO_0000100746

Regions

Domain138 – 21275POU-specific
DNA binding230 – 28960Homeobox

Amino acid modifications

Modified residue1111Phosphoserine; by MAPK By similarity
Modified residue2351Phosphothreonine By similarity
Modified residue2361Phosphoserine By similarity
Modified residue2891Phosphoserine By similarity
Modified residue2901Phosphoserine By similarity
Modified residue3551Phosphoserine By similarity
Cross-link123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence LengthMass (Da)Tools
O97552 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: DF0A326BE6329F08

FASTA36038,289
        10         20         30         40         50         60 
MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWMSFQG PPGGSGIGPG VVPGAEVWGL 

        70         80         90        100        110        120 
PPCPPPYDLC GGMAYCAPQV GVGPVPPGGL ETPQPEGEAG AGVESNSEGA SPDPCAAPAG 

       130        140        150        160        170        180 
APKLDKEKLE PNPEESQDIK ALQKDLEQFA KLLKQKRITL GYTQADVGLT LGVLFGKVFS 

       190        200        210        220        230        240 
QTTICRFEAL QLSFKNMCKL RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR 

       250        260        270        280        290        300 
VRGNLESMFL QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA 

       310        320        330        340        350        360 
AGSPFTGGPV SSPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEVFPSVSV TALGSPMHAN 

« Hide

References

[1]"Molecular cloning, genetic mapping, and developmental expression of bovine POU5F1."
van Eijk M.J.T., van Rooijen M.A., Modina S., Scesi L., Folkers G., van Tol H.T.A., Bevers M.M., Fisher S.R., Lewin H.A., Shehu D., Galli C., de Vaureix C., Trounson A.O., Mummery C.L., Gandolfi F.
Biol. Reprod. 60:1093-1103(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF022987, AF022986 Genomic DNA. Translation: AAD01757.1.
RefSeqNP_777005.1. NM_174580.2.
UniGeneBt.92603.

3D structure databases

ProteinModelPortalO97552.
SMRO97552. Positions 144-289.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO97552.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282316.
KEGGbta:282316.

Organism-specific databases

CTD5460.

Phylogenomic databases

eggNOGNOG329627.
HOGENOMHOG000089941.
HOVERGENHBG057998.
InParanoidO97552.
KOK09367.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5/6.
IPR000327. POU_specific.
[Graphical view]
PANTHERPTHR11636:SF15. PTHR11636:SF15. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806114.

Entry information

Entry namePO5F1_BOVIN
AccessionPrimary (citable) accession number: O97552
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: May 1, 1999
Last modified: February 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families