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O97552

- PO5F1_BOVIN

UniProt

O97552 - PO5F1_BOVIN

Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi230 – 28960HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1
    Alternative name(s):
    Octamer-binding protein 3
    Short name:
    Oct-3
    Octamer-binding transcription factor 3
    Short name:
    OTF-3
    Gene namesi
    Name:POU5F1
    Synonyms:OCT3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Expressed in a diffuse and slightly punctuate pattern.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360POU domain, class 5, transcription factor 1PRO_0000100746Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei111 – 1111Phosphoserine; by MAPKBy similarity
    Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei235 – 2351PhosphothreonineBy similarity
    Modified residuei236 – 2361PhosphoserineBy similarity
    Modified residuei289 – 2891PhosphoserineBy similarity
    Modified residuei290 – 2901PhosphoserineBy similarity
    Modified residuei355 – 3551PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.By similarity
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
    ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiO97552.

    Expressioni

    Tissue specificityi

    Expressed in immature oocytes.1 Publication

    Interactioni

    Subunit structurei

    Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO97552.
    SMRiO97552. Positions 144-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 21275POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The POU-specific domain mediates interaction with PKM.By similarity

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG329627.
    HOGENOMiHOG000089941.
    HOVERGENiHBG057998.
    InParanoidiO97552.
    KOiK09367.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015585. POU_dom_5.
    IPR000327. POU_specific.
    [Graphical view]
    PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O97552-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWMSFQG PPGGSGIGPG    50
    VVPGAEVWGL PPCPPPYDLC GGMAYCAPQV GVGPVPPGGL ETPQPEGEAG 100
    AGVESNSEGA SPDPCAAPAG APKLDKEKLE PNPEESQDIK ALQKDLEQFA 150
    KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL 200
    RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLESMFL 250
    QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA 300
    AGSPFTGGPV SSPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEVFPSVSV 350
    TALGSPMHAN 360
    Length:360
    Mass (Da):38,289
    Last modified:May 1, 1999 - v1
    Checksum:iDF0A326BE6329F08
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF022987, AF022986 Genomic DNA. Translation: AAD01757.1.
    RefSeqiNP_777005.1. NM_174580.2.
    UniGeneiBt.92603.

    Genome annotation databases

    GeneIDi282316.
    KEGGibta:282316.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF022987 , AF022986 Genomic DNA. Translation: AAD01757.1 .
    RefSeqi NP_777005.1. NM_174580.2.
    UniGenei Bt.92603.

    3D structure databases

    ProteinModelPortali O97552.
    SMRi O97552. Positions 144-289.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O97552.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 282316.
    KEGGi bta:282316.

    Organism-specific databases

    CTDi 5460.

    Phylogenomic databases

    eggNOGi NOG329627.
    HOGENOMi HOG000089941.
    HOVERGENi HBG057998.
    InParanoidi O97552.
    KOi K09367.

    Miscellaneous databases

    NextBioi 20806114.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015585. POU_dom_5.
    IPR000327. POU_specific.
    [Graphical view ]
    PANTHERi PTHR11636:SF15. PTHR11636:SF15. 1 hit.
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPO5F1_BOVIN
    AccessioniPrimary (citable) accession number: O97552
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2003
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3