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O97552

- PO5F1_BOVIN

UniProt

O97552 - PO5F1_BOVIN

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Protein
POU domain, class 5, transcription factor 1
Gene
POU5F1, OCT3
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi230 – 28960Homeobox
Add
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:POU5F1
Synonyms:OCT3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasm. Nucleus
Note: Expressed in a diffuse and slightly punctuate pattern By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360POU domain, class 5, transcription factor 1
PRO_0000100746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111Phosphoserine; by MAPK By similarity
Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Modified residuei235 – 2351Phosphothreonine By similarity
Modified residuei236 – 2361Phosphoserine By similarity
Modified residuei289 – 2891Phosphoserine By similarity
Modified residuei290 – 2901Phosphoserine By similarity
Modified residuei355 – 3551Phosphoserine By similarity

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation By similarity.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription By similarity.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiO97552.

Expressioni

Tissue specificityi

Expressed in immature oocytes.1 Publication

Interactioni

Subunit structurei

Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliO97552.
SMRiO97552. Positions 144-289.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 21275POU-specific
Add
BLAST

Domaini

The POU-specific domain mediates interaction with PKM By similarity.

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG329627.
HOGENOMiHOG000089941.
HOVERGENiHBG057998.
InParanoidiO97552.
KOiK09367.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view]
PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O97552-1 [UniParc]FASTAAdd to Basket

« Hide

MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWMSFQG PPGGSGIGPG    50
VVPGAEVWGL PPCPPPYDLC GGMAYCAPQV GVGPVPPGGL ETPQPEGEAG 100
AGVESNSEGA SPDPCAAPAG APKLDKEKLE PNPEESQDIK ALQKDLEQFA 150
KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL 200
RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLESMFL 250
QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYSQREDFEA 300
AGSPFTGGPV SSPLAPGPHF GTPGYGGPHF TTLYSSVPFP EGEVFPSVSV 350
TALGSPMHAN 360
Length:360
Mass (Da):38,289
Last modified:May 1, 1999 - v1
Checksum:iDF0A326BE6329F08
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF022987, AF022986 Genomic DNA. Translation: AAD01757.1.
RefSeqiNP_777005.1. NM_174580.2.
UniGeneiBt.92603.

Genome annotation databases

GeneIDi282316.
KEGGibta:282316.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF022987 , AF022986 Genomic DNA. Translation: AAD01757.1 .
RefSeqi NP_777005.1. NM_174580.2.
UniGenei Bt.92603.

3D structure databases

ProteinModelPortali O97552.
SMRi O97552. Positions 144-289.
ModBasei Search...

Proteomic databases

PRIDEi O97552.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 282316.
KEGGi bta:282316.

Organism-specific databases

CTDi 5460.

Phylogenomic databases

eggNOGi NOG329627.
HOGENOMi HOG000089941.
HOVERGENi HBG057998.
InParanoidi O97552.
KOi K09367.

Miscellaneous databases

NextBioi 20806114.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR015585. POU_dom_5.
IPR000327. POU_specific.
[Graphical view ]
PANTHERi PTHR11636:SF15. PTHR11636:SF15. 1 hit.
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.

Entry informationi

Entry nameiPO5F1_BOVIN
AccessioniPrimary (citable) accession number: O97552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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