Reviewed, UniProtKB/Swiss-Prot O97524 (BGLR_FELCA)

Names and origin

Protein names

Recommended name:
    Beta-glucuronidase
    EC=3.2.1.31

Gene names

Name:

GUSB

Organism

Felis silvestris catus (Cat)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays an important role in the degradation of dermatan and keratan sulfates.
Catalytic activity	A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.
Enzyme regulation	Inhibited by L-aspartic acid By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Lysosome.
Involvement in disease	Defects in GUSB are the cause of mucopolysaccharidosis type VII (MPS VII), an inherited disease reported in humans, mice, cats, and dogs. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Cellular component	Lysosome
Disease	Disease mutation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-glucuronidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 651	629	Beta-glucuronidase		PRO_0000012160
Sites
	Active site	450	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	419	1	N-linked (GlcNAc...) Potential
	Glycosylation	630	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	351	1	E → K in MPS VII. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O97524-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2AE30884B70D4232	FASTA	651	74,610
	10 20 30 40 50 60 MLRGPAAVWA ALGPLLWACG LALRGGMLYP RESPSRERKE LNGLWSFRAD FSENRRQGFE 70 80 90 100 110 120 QQWYRTPLRE SGPTLDMPVP SSFNDVGQDR QLRSFVGWVW YEREATLPQR WTQDLGTRVV 130 140 150 160 170 180 LRIGSAHYYA IVWVNGVHVA EHEGGHLPFE ADISKLVQSG PLASCRITIA INNTLTPHTL 190 200 210 220 230 240 PPGTILYQTD TSKYPKGYFV QNINFDFFNY AGLHRPVLLY TTPTTYIDDI TISTSVNQDT 250 260 270 280 290 300 GLVDYQIFVE GGEHFQLEVR LLDEEGKVVA QGTGGRGQLQ VPNAHLWWPY LMHEHPAYLY 310 320 330 340 350 360 SLEVRLTAQT AAGSVSDFYT LPVGIRTVAV TEHQFLINGK PFYFHGVNKH EDADIRGKGF 370 380 390 400 410 420 DWPLLVKDFN LLRWLGANAF RTSHYPYAEE VMQLCDRYGI VVIDESPGVG IVLVESYSNV 430 440 450 460 470 480 SLQHHLEVME ELVRRDKNHP AVVMWSVANE PASFLKPAGY YFKTLIAHTK ALDPSRPVTF 490 500 510 520 530 540 VTNSNYEADL GAPYVDVICV NSYYSWYHDY GHMEVIQLQL ATQFENWYRT YQKPIIQSEY 550 560 570 580 590 600 GADTIAGFHQ DPPLMFSEEY QKGLLEQYHL VLDQKRKEYV VGELIWNFAD FMTNQSPQRV 610 620 630 640 650 MGNKKGIFTR QRQPKGAAFL LRERYWKLAN ETRYPWSAVK SQCLENSPFT L

References

[1]

"Molecular basis of feline beta-glucuronidase deficiency: an animal model of mucopolysaccharidosis VII." Fyfe J.C., Kurzhals R.L., Lassaline M.E., Henthorn P.S., Alur P.R., Wang P., Wolfe J.H., Giger U., Haskins M.E., Patterson D.F., Sun H., Jain S., Yuhki N. Genomics 58:121-128(1999) [PubMed: 10366443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MPS VII LYS-351. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF012423 mRNA. Translation: AAD01498.1. AF012424 mRNA. Translation: AAD01499.1.
RefSeq	NP_001009310.1.
3D structure databases
HSSP	HSSP built from PDB template 1BHG based on UniProtKB P08236.
SMR	O97524. Positions 22-631.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Genome annotation databases
GeneID	493879.
Phylogenomic databases
HOVERGEN	O97524.
Enzyme and pathway databases
BRENDA	3.2.1.31. 273949.
Family and domain databases
InterPro	IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLR_FELCA

Accession

Primary (citable) accession number: O97524

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)