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O97524

- BGLR_FELCA

UniProt

O97524 - BGLR_FELCA

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Protein

Beta-glucuronidase

Gene

GUSB

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in the degradation of dermatan and keratan sulfates.

Catalytic activityi

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

Enzyme regulationi

Inhibited by L-aspartic acid.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei450 – 4501Proton donorBy similarity

GO - Molecular functioni

  1. beta-glucuronidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucuronidase (EC:3.2.1.31)
Gene namesi
Name:GUSB
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in GUSB are the cause of mucopolysaccharidosis type VII (MPS VII), an inherited disease reported in humans, mice, cats, and dogs.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 651629Beta-glucuronidasePRO_0000012160Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi630 – 6301N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000006431.

Structurei

3D structure databases

ProteinModelPortaliO97524.
SMRiO97524. Positions 22-631.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250.
HOVERGENiHBG004843.
InParanoidiO97524.
KOiK01195.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97524-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRGPAAVWA ALGPLLWACG LALRGGMLYP RESPSRERKE LNGLWSFRAD
60 70 80 90 100
FSENRRQGFE QQWYRTPLRE SGPTLDMPVP SSFNDVGQDR QLRSFVGWVW
110 120 130 140 150
YEREATLPQR WTQDLGTRVV LRIGSAHYYA IVWVNGVHVA EHEGGHLPFE
160 170 180 190 200
ADISKLVQSG PLASCRITIA INNTLTPHTL PPGTILYQTD TSKYPKGYFV
210 220 230 240 250
QNINFDFFNY AGLHRPVLLY TTPTTYIDDI TISTSVNQDT GLVDYQIFVE
260 270 280 290 300
GGEHFQLEVR LLDEEGKVVA QGTGGRGQLQ VPNAHLWWPY LMHEHPAYLY
310 320 330 340 350
SLEVRLTAQT AAGSVSDFYT LPVGIRTVAV TEHQFLINGK PFYFHGVNKH
360 370 380 390 400
EDADIRGKGF DWPLLVKDFN LLRWLGANAF RTSHYPYAEE VMQLCDRYGI
410 420 430 440 450
VVIDESPGVG IVLVESYSNV SLQHHLEVME ELVRRDKNHP AVVMWSVANE
460 470 480 490 500
PASFLKPAGY YFKTLIAHTK ALDPSRPVTF VTNSNYEADL GAPYVDVICV
510 520 530 540 550
NSYYSWYHDY GHMEVIQLQL ATQFENWYRT YQKPIIQSEY GADTIAGFHQ
560 570 580 590 600
DPPLMFSEEY QKGLLEQYHL VLDQKRKEYV VGELIWNFAD FMTNQSPQRV
610 620 630 640 650
MGNKKGIFTR QRQPKGAAFL LRERYWKLAN ETRYPWSAVK SQCLENSPFT

L
Length:651
Mass (Da):74,610
Last modified:May 1, 1999 - v1
Checksum:i2AE30884B70D4232
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti351 – 3511E → K in MPS VII. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012423 mRNA. Translation: AAD01498.1.
AF012424 mRNA. Translation: AAD01499.1.
RefSeqiNP_001009310.1. NM_001009310.1.

Genome annotation databases

GeneIDi493879.
KEGGifca:493879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012423 mRNA. Translation: AAD01498.1 .
AF012424 mRNA. Translation: AAD01499.1 .
RefSeqi NP_001009310.1. NM_001009310.1.

3D structure databases

ProteinModelPortali O97524.
SMRi O97524. Positions 22-631.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9685.ENSFCAP00000006431.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493879.
KEGGi fca:493879.

Organism-specific databases

CTDi 2990.

Phylogenomic databases

eggNOGi COG3250.
HOVERGENi HBG004843.
InParanoidi O97524.
KOi K01195.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SUPFAMi SSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular basis of feline beta-glucuronidase deficiency: an animal model of mucopolysaccharidosis VII."
    Fyfe J.C., Kurzhals R.L., Lassaline M.E., Henthorn P.S., Alur P.R., Wang P., Wolfe J.H., Giger U., Haskins M.E., Patterson D.F., Sun H., Jain S., Yuhki N.
    Genomics 58:121-128(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MPS VII LYS-351.
    Tissue: Liver.

Entry informationi

Entry nameiBGLR_FELCA
AccessioniPrimary (citable) accession number: O97524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3