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O97524

- BGLR_FELCA

UniProt

O97524 - BGLR_FELCA

Protein

Beta-glucuronidase

Gene

GUSB

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Plays an important role in the degradation of dermatan and keratan sulfates.

    Catalytic activityi

    A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

    Enzyme regulationi

    Inhibited by L-aspartic acid.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei450 – 4501Proton donorBy similarity

    GO - Molecular functioni

    1. beta-glucuronidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucuronidase (EC:3.2.1.31)
    Gene namesi
    Name:GUSB
    OrganismiFelis catus (Cat) (Felis silvestris catus)
    Taxonomic identifieri9685 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
    ProteomesiUP000011712: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Involvement in diseasei

    Defects in GUSB are the cause of mucopolysaccharidosis type VII (MPS VII), an inherited disease reported in humans, mice, cats, and dogs.

    Keywords - Diseasei

    Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222By similarityAdd
    BLAST
    Chaini23 – 651629Beta-glucuronidasePRO_0000012160Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi630 – 6301N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi9685.ENSFCAP00000006431.

    Structurei

    3D structure databases

    ProteinModelPortaliO97524.
    SMRiO97524. Positions 22-631.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3250.
    HOVERGENiHBG004843.
    KOiK01195.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SUPFAMiSSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O97524-1 [UniParc]FASTAAdd to Basket

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    MLRGPAAVWA ALGPLLWACG LALRGGMLYP RESPSRERKE LNGLWSFRAD    50
    FSENRRQGFE QQWYRTPLRE SGPTLDMPVP SSFNDVGQDR QLRSFVGWVW 100
    YEREATLPQR WTQDLGTRVV LRIGSAHYYA IVWVNGVHVA EHEGGHLPFE 150
    ADISKLVQSG PLASCRITIA INNTLTPHTL PPGTILYQTD TSKYPKGYFV 200
    QNINFDFFNY AGLHRPVLLY TTPTTYIDDI TISTSVNQDT GLVDYQIFVE 250
    GGEHFQLEVR LLDEEGKVVA QGTGGRGQLQ VPNAHLWWPY LMHEHPAYLY 300
    SLEVRLTAQT AAGSVSDFYT LPVGIRTVAV TEHQFLINGK PFYFHGVNKH 350
    EDADIRGKGF DWPLLVKDFN LLRWLGANAF RTSHYPYAEE VMQLCDRYGI 400
    VVIDESPGVG IVLVESYSNV SLQHHLEVME ELVRRDKNHP AVVMWSVANE 450
    PASFLKPAGY YFKTLIAHTK ALDPSRPVTF VTNSNYEADL GAPYVDVICV 500
    NSYYSWYHDY GHMEVIQLQL ATQFENWYRT YQKPIIQSEY GADTIAGFHQ 550
    DPPLMFSEEY QKGLLEQYHL VLDQKRKEYV VGELIWNFAD FMTNQSPQRV 600
    MGNKKGIFTR QRQPKGAAFL LRERYWKLAN ETRYPWSAVK SQCLENSPFT 650
    L 651
    Length:651
    Mass (Da):74,610
    Last modified:May 1, 1999 - v1
    Checksum:i2AE30884B70D4232
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti351 – 3511E → K in MPS VII. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012423 mRNA. Translation: AAD01498.1.
    AF012424 mRNA. Translation: AAD01499.1.
    RefSeqiNP_001009310.1. NM_001009310.1.

    Genome annotation databases

    GeneIDi493879.
    KEGGifca:493879.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF012423 mRNA. Translation: AAD01498.1 .
    AF012424 mRNA. Translation: AAD01499.1 .
    RefSeqi NP_001009310.1. NM_001009310.1.

    3D structure databases

    ProteinModelPortali O97524.
    SMRi O97524. Positions 22-631.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9685.ENSFCAP00000006431.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 493879.
    KEGGi fca:493879.

    Organism-specific databases

    CTDi 2990.

    Phylogenomic databases

    eggNOGi COG3250.
    HOVERGENi HBG004843.
    KOi K01195.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SUPFAMi SSF49303. SSF49303. 1 hit.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular basis of feline beta-glucuronidase deficiency: an animal model of mucopolysaccharidosis VII."
      Fyfe J.C., Kurzhals R.L., Lassaline M.E., Henthorn P.S., Alur P.R., Wang P., Wolfe J.H., Giger U., Haskins M.E., Patterson D.F., Sun H., Jain S., Yuhki N.
      Genomics 58:121-128(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MPS VII LYS-351.
      Tissue: Liver.

    Entry informationi

    Entry nameiBGLR_FELCA
    AccessioniPrimary (citable) accession number: O97524
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3