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O97524 (BGLR_FELCA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucuronidase

EC=3.2.1.31
Gene names
Name:GUSB
OrganismFelis catus (Cat) (Felis silvestris catus) [Reference proteome]
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis

Protein attributes

Sequence length651 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in the degradation of dermatan and keratan sulfates.

Catalytic activity

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

Enzyme regulation

Inhibited by L-aspartic acid By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Lysosome.

Involvement in disease

Defects in GUSB are the cause of mucopolysaccharidosis type VII (MPS VII), an inherited disease reported in humans, mice, cats, and dogs. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Cellular componentLysosome
   DiseaseDisease mutation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-glucuronidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 By similarity
Chain23 – 651629Beta-glucuronidase
PRO_0000012160

Sites

Active site4501Proton donor By similarity

Amino acid modifications

Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation4191N-linked (GlcNAc...) Potential
Glycosylation6301N-linked (GlcNAc...) Potential

Natural variations

Natural variant3511E → K in MPS VII. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O97524 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 2AE30884B70D4232

FASTA65174,610
        10         20         30         40         50         60 
MLRGPAAVWA ALGPLLWACG LALRGGMLYP RESPSRERKE LNGLWSFRAD FSENRRQGFE 

        70         80         90        100        110        120 
QQWYRTPLRE SGPTLDMPVP SSFNDVGQDR QLRSFVGWVW YEREATLPQR WTQDLGTRVV 

       130        140        150        160        170        180 
LRIGSAHYYA IVWVNGVHVA EHEGGHLPFE ADISKLVQSG PLASCRITIA INNTLTPHTL 

       190        200        210        220        230        240 
PPGTILYQTD TSKYPKGYFV QNINFDFFNY AGLHRPVLLY TTPTTYIDDI TISTSVNQDT 

       250        260        270        280        290        300 
GLVDYQIFVE GGEHFQLEVR LLDEEGKVVA QGTGGRGQLQ VPNAHLWWPY LMHEHPAYLY 

       310        320        330        340        350        360 
SLEVRLTAQT AAGSVSDFYT LPVGIRTVAV TEHQFLINGK PFYFHGVNKH EDADIRGKGF 

       370        380        390        400        410        420 
DWPLLVKDFN LLRWLGANAF RTSHYPYAEE VMQLCDRYGI VVIDESPGVG IVLVESYSNV 

       430        440        450        460        470        480 
SLQHHLEVME ELVRRDKNHP AVVMWSVANE PASFLKPAGY YFKTLIAHTK ALDPSRPVTF 

       490        500        510        520        530        540 
VTNSNYEADL GAPYVDVICV NSYYSWYHDY GHMEVIQLQL ATQFENWYRT YQKPIIQSEY 

       550        560        570        580        590        600 
GADTIAGFHQ DPPLMFSEEY QKGLLEQYHL VLDQKRKEYV VGELIWNFAD FMTNQSPQRV 

       610        620        630        640        650 
MGNKKGIFTR QRQPKGAAFL LRERYWKLAN ETRYPWSAVK SQCLENSPFT L 

« Hide

References

[1]"Molecular basis of feline beta-glucuronidase deficiency: an animal model of mucopolysaccharidosis VII."
Fyfe J.C., Kurzhals R.L., Lassaline M.E., Henthorn P.S., Alur P.R., Wang P., Wolfe J.H., Giger U., Haskins M.E., Patterson D.F., Sun H., Jain S., Yuhki N.
Genomics 58:121-128(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MPS VII LYS-351.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF012423 mRNA. Translation: AAD01498.1.
AF012424 mRNA. Translation: AAD01499.1.
RefSeqNP_001009310.1. NM_001009310.1.

3D structure databases

ProteinModelPortalO97524.
SMRO97524. Positions 22-631.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9685.ENSFCAP00000006431.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID493879.
KEGGfca:493879.

Organism-specific databases

CTD2990.

Phylogenomic databases

eggNOGCOG3250.
HOVERGENHBG004843.
KOK01195.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SUPFAMSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGLR_FELCA
AccessionPrimary (citable) accession number: O97524
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1999
Last modified: October 16, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries