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Protein

Inositol-3-phosphate synthase

Gene

Inos

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate.1 Publication

Cofactori

NAD+By similarity

Pathwayi: myo-inositol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Inositol-3-phosphate synthase (Inos)
  2. Putative inositol monophosphatase 3 (CG15743)
This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

GO - Molecular functioni

  • inositol-3-phosphate synthase activity Source: FlyBase

GO - Biological processi

  • inositol biosynthetic process Source: FlyBase
  • phospholipid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Inositol biosynthesis, Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-DME-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
UniPathwayiUPA00823; UER00787.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-3-phosphate synthase (EC:5.5.1.4)
Short name:
MIP synthase
Alternative name(s):
Myo-inositol 1-phosphate synthase
Short name:
IPS
Short name:
MI-1-P synthase
Gene namesi
Name:Inos
ORF Names:CG11143
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0025885. Inos.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951821 – 565Inositol-3-phosphate synthaseAdd BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei536Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO97477.
PRIDEiO97477.

PTM databases

iPTMnetiO97477.

Expressioni

Tissue specificityi

Higher expression in adult heads than bodies.1 Publication

Gene expression databases

BgeeiFBgn0025885.
GenevisibleiO97477. DM.

Interactioni

Protein-protein interaction databases

BioGridi61549. 62 interactors.
STRINGi7227.FBpp0088368.

Structurei

3D structure databases

ProteinModelPortaliO97477.
SMRiO97477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0693. Eukaryota.
COG1260. LUCA.
GeneTreeiENSGT00390000018395.
InParanoidiO97477.
KOiK01858.
OMAiNPVLYAP.
OrthoDBiEOG091G05DF.
PhylomeDBiO97477.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002587. Myo-inos-1-P_Synthase.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11510. PTHR11510. 1 hit.
PfamiPF01658. Inos-1-P_synth. 1 hit.
PF07994. NAD_binding_5. 1 hit.
[Graphical view]
PIRSFiPIRSF015578. Myoinos-ppht_syn. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.

Sequencei

Sequence statusi: Complete.

O97477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPTNNSTLE VISPKVQVDD EFITTDYDYQ TSHVKRTADG QLQVHPQTTS
60 70 80 90 100
LKIRTGRHVP KLGVMLVGWG GNNGSTLTAA LEANRRQLKW RKRTGVQEAN
110 120 130 140 150
WYGSITQAST VFIGSDEDGG DVYVPMKELL PMVEPDNIIV DGWDISGLHL
160 170 180 190 200
GDAMRRAEVL DVALQDQIYD QLAQLRPRPS IYDPDFIAAN QSDRADNVIR
210 220 230 240 250
GTRLEQYEQI RKDIRDFRER SGVDSVIVLW TANTERFADV QPGLNTTSQE
260 270 280 290 300
LIASLEANHS EVSPSTIFAM ASIAEGCTYI NGSPQNTFVP GLIQLAEEKN
310 320 330 340 350
VFIAGDDFKS GQTKIKSVLV DFLVGAGIKP VSIASYNHLG NNDGKNLSAP
360 370 380 390 400
QQFRSKEISK SNVVDDMVAS NRLLYGPDEH PDHVVVIKYV PYVGDSKRAM
410 420 430 440 450
DEYTSEIMMG GHNTLVIHNT CEDSLLATPL ILDLVILGEL STRIQLRNAE
460 470 480 490 500
KESAPWVPFK PVLSLLSYLC KAPLVPQGSQ VVNSLFRQRA AIENILRGCI
510 520 530 540 550
GLPPISHMTL EQRFDFSTIT NEPPLKRVKI LGQPCSVESV TNGKKLHANG
560
HSNGSAKLAT NGNGH
Length:565
Mass (Da):62,249
Last modified:May 1, 1999 - v1
Checksum:i6CF53D0D27BE186F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti324V → L in AAN71315 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071103 mRNA. Translation: AAD02819.1.
AF071104 Genomic DNA. Translation: AAD13140.1.
AE013599 Genomic DNA. Translation: AAF59252.1.
AY122137 mRNA. Translation: AAM52649.1.
BT001560 mRNA. Translation: AAN71315.1. Sequence problems.
BT001772 mRNA. Translation: AAN71527.1.
RefSeqiNP_477405.1. NM_058057.6.
UniGeneiDm.3378.

Genome annotation databases

EnsemblMetazoaiFBtr0089329; FBpp0088368; FBgn0025885.
GeneIDi35671.
KEGGidme:Dmel_CG11143.
UCSCiCG11143-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071103 mRNA. Translation: AAD02819.1.
AF071104 Genomic DNA. Translation: AAD13140.1.
AE013599 Genomic DNA. Translation: AAF59252.1.
AY122137 mRNA. Translation: AAM52649.1.
BT001560 mRNA. Translation: AAN71315.1. Sequence problems.
BT001772 mRNA. Translation: AAN71527.1.
RefSeqiNP_477405.1. NM_058057.6.
UniGeneiDm.3378.

3D structure databases

ProteinModelPortaliO97477.
SMRiO97477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61549. 62 interactors.
STRINGi7227.FBpp0088368.

PTM databases

iPTMnetiO97477.

Proteomic databases

PaxDbiO97477.
PRIDEiO97477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089329; FBpp0088368; FBgn0025885.
GeneIDi35671.
KEGGidme:Dmel_CG11143.
UCSCiCG11143-RA. d. melanogaster.

Organism-specific databases

CTDi35671.
FlyBaseiFBgn0025885. Inos.

Phylogenomic databases

eggNOGiKOG0693. Eukaryota.
COG1260. LUCA.
GeneTreeiENSGT00390000018395.
InParanoidiO97477.
KOiK01858.
OMAiNPVLYAP.
OrthoDBiEOG091G05DF.
PhylomeDBiO97477.

Enzyme and pathway databases

UniPathwayiUPA00823; UER00787.
ReactomeiR-DME-1855183. Synthesis of IP2, IP, and Ins in the cytosol.

Miscellaneous databases

GenomeRNAii35671.
PROiO97477.

Gene expression databases

BgeeiFBgn0025885.
GenevisibleiO97477. DM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002587. Myo-inos-1-P_Synthase.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11510. PTHR11510. 1 hit.
PfamiPF01658. Inos-1-P_synth. 1 hit.
PF07994. NAD_binding_5. 1 hit.
[Graphical view]
PIRSFiPIRSF015578. Myoinos-ppht_syn. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiINO1_DROME
AccessioniPrimary (citable) accession number: O97477
Secondary accession number(s): Q8IGW1, Q8MR42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.