ID   GPDA_DROKA              Reviewed;         360 AA.
AC   O97463; Q9TVF9; Q9TVK5;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic;
DE            Short=GPDH-C;
DE            Short=GPD-C;
DE            EC=1.1.1.8;
GN   Name=Gpdh1;
GN   and
GN   Name=Gpdh2;
OS   Drosophila kanekoi (Fruit fly).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila.
OX   NCBI_TaxID=50033;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Asyoro;
RA   Kitagawa H.;
RL   Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(+) = glycerone
CC       phosphate + NADH.
CC   -!- PATHWAY: Phospholipid metabolism; alpha-glycerophosphate cycle.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=O97463-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O97463-2; Sequence=VSP_001587;
CC       Name=3;
CC         IsoId=O97463-3; Sequence=VSP_001588;
CC   -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; AB019506; BAA34356.1; -; Genomic_DNA.
DR   EMBL; AB019506; BAA34357.1; -; Genomic_DNA.
DR   EMBL; AB019506; BAA34358.1; -; Genomic_DNA.
DR   EMBL; AB019507; BAA34359.1; -; Genomic_DNA.
DR   EMBL; AB019507; BAA34360.1; -; Genomic_DNA.
DR   EMBL; AB019507; BAA34361.1; -; Genomic_DNA.
DR   FlyBase; FBgn0044992; Dkan\Gpdh1.
DR   FlyBase; FBgn0044991; Dkan\Gpdh2.
DR   BRENDA; 1.1.1.8; 316327.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase (NAD+) a...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006168; NAD-dep_Gly3P_DH.
DR   InterPro; IPR017751; NAD-dep_Gly3P_DH_euk.
DR   InterPro; IPR011128; NAD-dep_Gly3P_DH_N.
DR   InterPro; IPR006109; NAD_Gly3P_DH_C.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR11728; NAD_Gly3P_DH; 1.
DR   Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR   PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR   PRINTS; PR00077; GPDHDRGNASE.
DR   ProDom; PD001278; NAD_Gly3P_C; 1.
DR   TIGRFAMs; TIGR03376; glycerol3P_DH; 1.
DR   PROSITE; PS00957; NAD_G3PDH; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Cytoplasm; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    360       Glycerol-3-phosphate dehydrogenase
FT                                [NAD+], cytoplasmic.
FT                                /FTId=PRO_0000138074.
FT   NP_BIND      11     16       NAD (By similarity).
FT   REGION      270    271       Substrate binding (By similarity).
FT   ACT_SITE    206    206       Proton acceptor (By similarity).
FT   BINDING      98     98       NAD (By similarity).
FT   BINDING     121    121       NAD; via amide nitrogen (By similarity).
FT   BINDING     121    121       Substrate (By similarity).
FT   BINDING     155    155       NAD; via amide nitrogen (By similarity).
FT   BINDING     270    270       NAD (By similarity).
FT   BINDING     299    299       NAD (By similarity).
FT   VAR_SEQ     351    360       DTFIMPSPKL -> QTL (in isoform 2).
FT                                /FTId=VSP_001587.
FT   VAR_SEQ     351    360       Missing (in isoform 3).
FT                                /FTId=VSP_001588.
SQ   SEQUENCE   360 AA;  39465 MW;  32040734744C3372 CRC64;
     MAEKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEEMIDGKK LTEIINETHE
     NVKYLKGHKL PTNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI
     KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR
     DLFQANHFRV VVVEDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF
     VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG
     PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC INQLKPKDLI DCIRNHPEHM DTFIMPSPKL
//