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O97463 (GPDA_DROKA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic

Short name=GPD-C
Short name=GPDH-C
EC=1.1.1.8
Gene names
Name:Gpdh1
AND
Name:Gpdh2
OrganismDrosophila kanekoi (Fruit fly)
Taxonomic identifier50033 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Pathway

Phospholipid metabolism; alpha-glycerophosphate cycle.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O97463-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O97463-2)

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: DTFIMPSPKL → QTL
Isoform 3 (identifier: O97463-3)

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 360359Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic
PRO_0000138074

Regions

Nucleotide binding11 – 166NAD By similarity
Region270 – 2712Substrate binding By similarity

Sites

Active site2061Proton acceptor By similarity
Binding site981NAD By similarity
Binding site1211NAD; via amide nitrogen By similarity
Binding site1211Substrate By similarity
Binding site1551NAD; via amide nitrogen By similarity
Binding site2701NAD By similarity
Binding site2991NAD By similarity

Natural variations

Alternative sequence351 – 36010DTFIMPSPKL → QTL in isoform 2.
VSP_001587
Alternative sequence351 – 36010Missing in isoform 3.
VSP_001588

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 32040734744C3372

FASTA36039,465
        10         20         30         40         50         60 
MAEKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 

        70         80         90        100        110        120 
NVKYLKGHKL PTNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 

       130        140        150        160        170        180 
KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 

       190        200        210        220        230        240 
DLFQANHFRV VVVEDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 

       250        260        270        280        290        300 
VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 

       310        320        330        340        350        360 
PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC INQLKPKDLI DCIRNHPEHM DTFIMPSPKL 

« Hide

Isoform 2 [UniParc].

Checksum: CC8B4C4893027CA8
Show »

FASTA35338,677
Isoform 3 [UniParc].

Checksum: F893027CA8D7D660
Show »

FASTA35038,335

References

[1]Kitagawa H.
Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Asyoro.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB019506 Genomic DNA. Translation: BAA34356.1.
AB019506 Genomic DNA. Translation: BAA34357.1.
AB019506 Genomic DNA. Translation: BAA34358.1.
AB019507 Genomic DNA. Translation: BAA34359.1.
AB019507 Genomic DNA. Translation: BAA34360.1.
AB019507 Genomic DNA. Translation: BAA34361.1.

3D structure databases

ProteinModelPortalO97463.
SMRO97463. Positions 1-350.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

FlyBaseFBgn0044992. Dkan\Gpdh1.
FBgn0044991. Dkan\Gpdh2.

Enzyme and pathway databases

UniPathwayUPA00086.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR03376. glycerol3P_DH. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGPDA_DROKA
AccessionPrimary (citable) accession number: O97463
Secondary accession number(s): Q9TVF9, Q9TVK5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase