Reviewed, UniProtKB/Swiss-Prot O97463 (GPDA_DROKA)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic
      Short name=GPDH-C
      Short name=GPD-C
    EC=1.1.1.8

Gene names

Name:	Gpdh1
AND
Name:	Gpdh2

Organism

Drosophila kanekoi (Fruit fly)

Taxonomic identifier

50033 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Pathway	Phospholipid metabolism; alpha-glycerophosphate cycle.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O97463-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O97463-2)

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: DTFIMPSPKL → QTL

Isoform 3 (identifier: O97463-3)

The sequence of this isoform differs from the canonical sequence as follows:
     351-360: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 360	359	Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic		PRO_0000138074
Regions
	Nucleotide binding	11 – 16	6	NAD By similarity
	Region	270 – 271	2	Substrate binding By similarity
Sites
	Active site	206	1	Proton acceptor By similarity
	Binding site	98	1	NAD By similarity
	Binding site	121	1	NAD; via amide nitrogen By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	155	1	NAD; via amide nitrogen By similarity
	Binding site	270	1	NAD By similarity
	Binding site	299	1	NAD By similarity
Natural variations
	Alternative sequence	351 – 360	10	DTFIMPSPKL → QTL in isoform 2.		VSP_001587
	Alternative sequence	351 – 360	10	Missing in isoform 3.		VSP_001588

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 32040734744C3372	FASTA	360	39,465
	10 20 30 40 50 60 MAEKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 70 80 90 100 110 120 NVKYLKGHKL PTNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 130 140 150 160 170 180 KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 190 200 210 220 230 240 DLFQANHFRV VVVEDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 250 260 270 280 290 300 VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 310 320 330 340 350 360 PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC INQLKPKDLI DCIRNHPEHM DTFIMPSPKL
	Isoform 2. Checksum: CC8B4C4893027CA8	FASTA	353	38,677
	10 20 30 40 50 60 MAEKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 70 80 90 100 110 120 NVKYLKGHKL PTNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 130 140 150 160 170 180 KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 190 200 210 220 230 240 DLFQANHFRV VVVEDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 250 260 270 280 290 300 VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 310 320 330 340 350 PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC INQLKPKDLI DCIRNHPEHM QTL
	Isoform 3. Checksum: F893027CA8D7D660	FASTA	350	38,335
	10 20 30 40 50 60 MAEKVNVCIV GSGNWGSAIA KIVGANAAAL PEFEERVTMF VYEEMIDGKK LTEIINETHE 70 80 90 100 110 120 NVKYLKGHKL PTNVVAVPDL VEAAKNADIL IFVVPHQFIP NFCKQLLGKI KPNAIAISLI 130 140 150 160 170 180 KGFDKAEGGG IDLISHIITR HLKIPCAVLM GANLANEVAE GNFCETTIGC TDKKYGKVLR 190 200 210 220 230 240 DLFQANHFRV VVVEDADAVE VCGALKNIVA CGAGFVDGLK LGDNTKAAVI RLGLMEMIRF 250 260 270 280 290 300 VDVFYPGSKL STFFESCGVA DLITTCYGGR NRRVSEAFVT SGKTIEELEK EMLNGQKLQG 310 320 330 340 350 PPTAEEVNYM LKNKGLEDKF PLFTAIHKIC INQLKPKDLI DCIRNHPEHM

References

[1]	Kitagawa H. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Asyoro.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB019506 Genomic DNA. Translation: BAA34356.1. AB019506 Genomic DNA. Translation: BAA34357.1. AB019506 Genomic DNA. Translation: BAA34358.1. AB019507 Genomic DNA. Translation: BAA34359.1. AB019507 Genomic DNA. Translation: BAA34360.1. AB019507 Genomic DNA. Translation: BAA34361.1.
3D structure databases
ModBase	Search...
Organism-specific databases
FlyBase	FBgn0044992. Dkan\Gpdh1. FBgn0044991. Dkan\Gpdh2.
Enzyme and pathway databases
BRENDA	1.1.1.8. 316327.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDA_DROKA

Accession

Primary (citable) accession number: O97463
Secondary accession number(s): Q9TVF9, Q9TVK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project