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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase I

Gene

GlcAT-I

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates.1 Publication

Catalytic activityi

UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.By similarity

Cofactori

Mn2+1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi163ManganeseBy similarity1
Active sitei252Proton acceptorBy similarity1

GO - Molecular functioni

  • galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Source: FlyBase
  • glucuronosyltransferase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionTransferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase I (EC:2.4.1.135By similarity)
Alternative name(s):
Beta-1,3-glucuronyltransferase I
Glucuronosyltransferase I
Short name:
DmGlcAT-I
UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase
Short name:
GlcUAT-I
Gene namesi
Name:GlcAT-I
ORF Names:CG32775
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0066114. GlcAT-I.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini30 – 306LumenalSequence analysisAdd BLAST277

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951791 – 306Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase IAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO97422.
PRIDEiO97422.

Expressioni

Developmental stagei

Expressed at low levels from early embryos to adults; maximal expression in third instar larvae.1 Publication

Gene expression databases

BgeeiFBgn0066114.
ExpressionAtlasiO97422. differential.
GenevisibleiO97422. DM.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0070612.

Structurei

3D structure databases

ProteinModelPortaliO97422.
SMRiO97422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
InParanoidiO97422.
KOiK10158.
OMAiFHTAWEP.
OrthoDBiEOG091G0G8P.
PhylomeDBiO97422.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiView protein in Pfam
PF03360. Glyco_transf_43. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O97422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVRIRPRQ VLILIIVFLV VLMMVHRNGK RTCQGPEYLQ AMFVQGDTLP
60 70 80 90 100
TIYAVTPTYP RPAQKAELTR LSHLFMLLPH LHWIIVEDTN ATTPLVRNLL
110 120 130 140 150
DRAGLEKRST LLNIKTPSEF KLKGKDPNWI KPRGVEQRNL ALAWLRNHVD
160 170 180 190 200
VDRHSIVFFM DDDNSYSTEL FAEMSKIERG RVGVWPVGLV GGLMVERPLL
210 220 230 240 250
TEDGTKVTGF NAAWRPERPF PIDMAAFAIS MDLFIRNPQA TFSYEVQRGY
260 270 280 290 300
QESEILRHLT TRDQLQPLAN RCTDVLVWHT RTEKTKLAAE EALLKKGQRS

DGGMEV
Length:306
Mass (Da):35,072
Last modified:November 7, 2003 - v2
Checksum:i69910A46534218B0
GO

Sequence cautioni

The sequence CAA21824 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55V → L (PubMed:12511570).Curated1
Sequence conflicti55V → L (PubMed:10731137).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB080695 mRNA. Translation: BAC65095.1.
AE014298 Genomic DNA. Translation: AAN09117.1.
AL033125 Genomic DNA. Translation: CAA21824.1. Sequence problems.
AY060634 mRNA. Translation: AAL28182.1.
RefSeqiNP_726910.1. NM_167003.3.
UniGeneiDm.3928.

Genome annotation databases

EnsemblMetazoaiFBtr0070644; FBpp0070612; FBgn0066114.
GeneIDi251900.
KEGGidme:Dmel_CG32775.
UCSCiCG32775-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiB3GI_DROME
AccessioniPrimary (citable) accession number: O97422
Secondary accession number(s): Q8IRS6, Q95SR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 7, 2003
Last modified: August 30, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families