O97422 (B3GI_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase I
EC=2.4.1.135

Alternative name(s):
Beta-1,3-glucuronyltransferase I
Glucuronosyltransferase I
Short name=DmGlcAT-I
UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase
Short name=GlcUAT-I

Gene names

Name:	GlcAT-I
ORF Names:	CG32775

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates. Ref.1
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Developmental stage	Expressed at low levels from early embryos to adults; maximal expression in third instar larvae. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 43 family.
Sequence caution	The sequence CAA21824.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteoglycan biosynthetic process Non-traceable author statement Ref.1. Source: FlyBase
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from direct assay Ref.1. Source: FlyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase I		PRO_0000195179
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 29	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 306	277	Lumenal Potential
Sites
	Active site	252	1	Proton acceptor By similarity
	Metal binding	163	1	Manganese By similarity
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	55	1	V → L Ref.1
	Sequence conflict	55	1	V → L Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O97422 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: 69910A46534218B0	FASTA	306	35,072
	10 20 30 40 50 60 MSEVRIRPRQ VLILIIVFLV VLMMVHRNGK RTCQGPEYLQ AMFVQGDTLP TIYAVTPTYP 70 80 90 100 110 120 RPAQKAELTR LSHLFMLLPH LHWIIVEDTN ATTPLVRNLL DRAGLEKRST LLNIKTPSEF 130 140 150 160 170 180 KLKGKDPNWI KPRGVEQRNL ALAWLRNHVD VDRHSIVFFM DDDNSYSTEL FAEMSKIERG 190 200 210 220 230 240 RVGVWPVGLV GGLMVERPLL TEDGTKVTGF NAAWRPERPF PIDMAAFAIS MDLFIRNPQA 250 260 270 280 290 300 TFSYEVQRGY QESEILRHLT TRDQLQPLAN RCTDVLVWHT RTEKTKLAAE EALLKKGQRS DGGMEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of three Drosophila melanogaster glucuronyltransferases responsible for the synthesis of the conserved glycosaminoglycan-protein linkage region of proteoglycans: two novel homologs exhibit broad specificity toward oligosaccharides from proteoglycans, glycoproteins, and glycosphingolipids." Kim B.-T., Tsuchida K., Lincecum J., Kitagawa K., Bernfield M., Sugahara K. J. Biol. Chem. 278:9116-9124(2003) [PubMed: 12511570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, COFACTOR, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"From sequence to chromosome: the tip of the X chromosome of D. melanogaster." Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D., Minana B. , Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S., McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C., Glover D.M. Science 287:2220-2222(2000) [PubMed: 10731137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Oregon-R.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB080695 mRNA. Translation: BAC65095.1. AE014298 Genomic DNA. Translation: AAN09117.1. AL033125 Genomic DNA. Translation: CAA21824.1. Sequence problems. AY060634 mRNA. Translation: AAL28182.1.
RefSeq	NP_726910.1. NM_167003.2.
UniGene	Dm.3928.
3D structure databases
ProteinModelPortal	O97422.
SMR	O97422. Positions 48-306.
ModBase	Search...
Protein-protein interaction databases
STRING	O97422.
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0070644; FBpp0070612; FBgn0066114.
GeneID	251900.
KEGG	dme:Dmel_CG32775.
NMPDR	fig|7227.3.peg.16447.
UCSC	CG32775-RA. d. melanogaster.
Organism-specific databases
CTD	251900.
FlyBase	FBgn0066114. GlcAT-I.
Phylogenomic databases
eggNOG	inNOG05045.
GeneTree	EMGT00050000013702.
InParanoid	O97422.
OMA	SAWRPER.
OrthoDB	EOG451C6H.
PhylomeDB	O97422.
Gene expression databases
Bgee	O97422.
GermOnline	CG32775. Drosophila melanogaster.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
KO	K10158.
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	843575.

Entry information

Entry name

B3GI_DROME

Accession

Primary (citable) accession number: O97422
Secondary accession number(s): Q8IRS6, Q95SR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 7, 2003
Last modified:	January 25, 2012
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project