Reviewed, UniProtKB/Swiss-Prot O97402 (XYN_PHACE)

Names and origin

Protein names	Recommended name:     Endo-1,4-beta-xylanase       Short name=Xylanase     EC=3.2.1.8 Alternative name(s):     1,4-beta-D-xylan xylanohydrolase
Organism	Phaedon cochleariae (Mustard beetle)
Taxonomic identifier	80249 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Chrysomelini › Phaedon

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation. UniProtKB P36217
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in larval carcasses and gut, and adult gut. Ref.1
Developmental stage	Larvae and adult. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 11 (cellulase G) family.

Ontologies

Keywords
Biological process	Digestion Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 217	200	Endo-1,4-beta-xylanase		PRO_5000147330
Sites
	Active site	107	1	Nucleophile By similarity UniProtKB P36217
	Active site	204	1	Proton donor By similarity UniProtKB P36217
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	80	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O97402-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 92185C41BF39A644	FASTA	217	23,942
	10 20 30 40 50 60 MQFLIPVVIL CVSLVDSQKV LYNNEIGFNN GFYYAFWKDS GSATFTLESG GRYAGNWTTS 70 80 90 100 110 120 TNNWVGGKGW NPGNSWRTVN YSGYYGINEY ANSYLSLYGW TTNPLIEYYV VESYGSYSPL 130 140 150 160 170 180 NCPGGTDEGS FTSGGATYQV RKCRRTNAPS IIGTQSFDQY FSVRTPKKGF GQVSGSVNFA 190 200 210 DHVQYWASKG LPLGTHAHQI FATEGYQSSG FADITVS

References

[1]

"Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae." Girard C., Jouanin L. Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed: 10612046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Larval gut.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y17908 mRNA. Translation: CAA76932.1.
3D structure databases
HSSP	HSSP built from PDB template 1XYO based on UniProtKB P36217.
SMR	O97402. Positions 18-217.
ModBase	Search...
Protein family/group databases
CAZy	GH11. Glycoside Hydrolase Family 11.
Enzyme and pathway databases
BRENDA	3.2.1.8. 290922.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001137. Glyco_hydro_11. IPR013319. Glyco_hydro_11/12_cat. IPR018208. Glyco_hydro_11_AS. [Graphical view]
Gene3D	G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.
Pfam	PF00457. Glyco_hydro_11. 1 hit. [Graphical view]
PRINTS	PR00911. GLHYDRLASE11.
PROSITE	PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit. PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYN_PHACE

Accession

Primary (citable) accession number: O97402
Secondary accession number(s): P81525

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 1999
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)