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O97401

- GUN_PHACE

UniProt

O97401 - GUN_PHACE

Protein

Endoglucanase

Gene
N/A
Organism
Phaedon cochleariae (Mustard beetle)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei47 – 471NucleophileBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW
    2. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Digestion, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH45. Glycoside Hydrolase Family 45.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase (EC:3.2.1.4)
    Alternative name(s):
    Cellulase
    Endo-1,4-beta-glucanase
    OrganismiPhaedon cochleariae (Mustard beetle)
    Taxonomic identifieri80249 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeChrysomeliniPhaedon

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 242221EndoglucanaseSequence AnalysisPRO_5000147329Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed in larval carcasses and gut, and adult gut.1 Publication

    Developmental stagei

    Eggs, larvae and adult.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO97401.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 45 (cellulase K) family.Sequence Analysis

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.40.40.10. 1 hit.
    InterProiIPR014733. Barwin-like_endoglucanase.
    IPR000334. Glyco_hydro_45.
    IPR009009. RlpA-like_DPBB.
    [Graphical view]
    PfamiPF02015. Glyco_hydro_45. 1 hit.
    [Graphical view]
    SUPFAMiSSF50685. SSF50685. 1 hit.
    PROSITEiPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O97401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQVIVLPLVF LATFATSGSL AAPDASPEIV PVDGGLSGYG TTTRYWDCCK    50
    PSCAWKENIN TPTMTPVQTC AIDGNTVVNA SVQSGCIGGS SYMCSNQQAF 100
    VVNSTLAFGF AAGSFTGGVD NNLCCSCMLL TFQGQLAGKQ FLVQITNTGG 150
    DLGSTSSIWP FPGGGVGIFT QGCHDQWTPR GAAGGDQYGG VYSVEQCSDL 200
    PEVLQPGCRF RFEFLENVSN PQVSFQQVQC PAEIVAISNC AL 242
    Length:242
    Mass (Da):25,336
    Last modified:May 1, 1999 - v1
    Checksum:iE57A9EC9DAE4379D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17907 mRNA. Translation: CAA76931.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y17907 mRNA. Translation: CAA76931.1 .

    3D structure databases

    ProteinModelPortali O97401.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH45. Glycoside Hydrolase Family 45.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.40.40.10. 1 hit.
    InterProi IPR014733. Barwin-like_endoglucanase.
    IPR000334. Glyco_hydro_45.
    IPR009009. RlpA-like_DPBB.
    [Graphical view ]
    Pfami PF02015. Glyco_hydro_45. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50685. SSF50685. 1 hit.
    PROSITEi PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae."
      Girard C., Jouanin L.
      Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Larval gut1 Publication.

    Entry informationi

    Entry nameiGUN_PHACE
    AccessioniPrimary (citable) accession number: O97401
    Secondary accession number(s): P81522
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3