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O97401 (GUN_PHACE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase

EC=3.2.1.4
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
OrganismPhaedon cochleariae (Mustard beetle)
Taxonomic identifier80249 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeChrysomeliniPhaedon

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted Potential.

Tissue specificity

Expressed in larval carcasses and gut, and adult gut. Ref.1

Developmental stage

Eggs, larvae and adult. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Digestion
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

digestion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 242221Endoglucanase
PRO_5000147329

Sites

Active site471Nucleophile By similarity UniProtKB P43316

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation1031N-linked (GlcNAc...) Potential
Glycosylation2171N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O97401 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: E57A9EC9DAE4379D

FASTA24225,336
        10         20         30         40         50         60 
MQVIVLPLVF LATFATSGSL AAPDASPEIV PVDGGLSGYG TTTRYWDCCK PSCAWKENIN 

        70         80         90        100        110        120 
TPTMTPVQTC AIDGNTVVNA SVQSGCIGGS SYMCSNQQAF VVNSTLAFGF AAGSFTGGVD 

       130        140        150        160        170        180 
NNLCCSCMLL TFQGQLAGKQ FLVQITNTGG DLGSTSSIWP FPGGGVGIFT QGCHDQWTPR 

       190        200        210        220        230        240 
GAAGGDQYGG VYSVEQCSDL PEVLQPGCRF RFEFLENVSN PQVSFQQVQC PAEIVAISNC 


AL 

« Hide

References

[1]"Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae."
Girard C., Jouanin L.
Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Larval gut.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y17907 mRNA. Translation: CAA76931.1.

3D structure databases

ProteinModelPortalO97401.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH45. Glycoside Hydrolase Family 45.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.40.40.10. 1 hit.
InterProIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMSSF50685. SSF50685. 1 hit.
PROSITEPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN_PHACE
AccessionPrimary (citable) accession number: O97401
Secondary accession number(s): P81522
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: October 16, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries