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Protein

Endoglucanase

Gene
N/A
Organism
Phaedon cochleariae (Mustard beetle)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei47 – 471NucleophilePROSITE-ProRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Digestion, Polysaccharide degradation

Protein family/group databases

CAZyiGH45. Glycoside Hydrolase Family 45.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
OrganismiPhaedon cochleariae (Mustard beetle)
Taxonomic identifieri80249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeChrysomeliniPhaedon

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 242221EndoglucanaseSequence AnalysisPRO_5000147329Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in larval carcasses and gut, and adult gut.1 Publication

Developmental stagei

Eggs, larvae and adult.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO97401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 45 (cellulase K) family.Sequence Analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
InterProiIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVIVLPLVF LATFATSGSL AAPDASPEIV PVDGGLSGYG TTTRYWDCCK
60 70 80 90 100
PSCAWKENIN TPTMTPVQTC AIDGNTVVNA SVQSGCIGGS SYMCSNQQAF
110 120 130 140 150
VVNSTLAFGF AAGSFTGGVD NNLCCSCMLL TFQGQLAGKQ FLVQITNTGG
160 170 180 190 200
DLGSTSSIWP FPGGGVGIFT QGCHDQWTPR GAAGGDQYGG VYSVEQCSDL
210 220 230 240
PEVLQPGCRF RFEFLENVSN PQVSFQQVQC PAEIVAISNC AL
Length:242
Mass (Da):25,336
Last modified:May 1, 1999 - v1
Checksum:iE57A9EC9DAE4379D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17907 mRNA. Translation: CAA76931.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17907 mRNA. Translation: CAA76931.1.

3D structure databases

ProteinModelPortaliO97401.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH45. Glycoside Hydrolase Family 45.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
InterProiIPR014733. Barwin-like_endoglucanase.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae."
    Girard C., Jouanin L.
    Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Larval gut1 Publication.

Entry informationi

Entry nameiGUN_PHACE
AccessioniPrimary (citable) accession number: O97401
Secondary accession number(s): P81522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.