Reviewed, UniProtKB/Swiss-Prot O97400 (PGLR_PHACE)

Names and origin

Protein names	Recommended name:     Polygalacturonase       Short name=PG     EC=3.2.1.15 Alternative name(s):     Pectinase
Organism	Phaedon cochleariae (Mustard beetle)
Taxonomic identifier	80249 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Chrysomelini › Phaedon

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Potential. Secreted › cell wall Potential.
Tissue specificity	Expressed in larval carcasses and gut, and adult gut. Ref.1
Developmental stage	Eggs, larvae and adult. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 2 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Digestion
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW digestion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – ?		Potential		PRO_0000316163
	Chain	? – 367		Polygalacturonase		PRO_5000147328
Regions
	Repeat	187 – 208	22	PbH1 1
	Repeat	240 – 261	22	PbH1 2
Sites
	Active site	201	1	Proton donor By similarity UniProtKB O74213
	Active site	223	1	By similarity UniProtKB O74213
Amino acid modifications
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Glycosylation	242	1	N-linked (GlcNAc...) Potential
	Glycosylation	343	1	N-linked (GlcNAc...) Potential
	Glycosylation	357	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 42		By similarity UniProtKB O74213
	Disulfide bond	203 ↔ 219		By similarity UniProtKB O74213
	Disulfide bond	334 ↔ 339		By similarity UniProtKB O74213
	Disulfide bond	358 ↔ 367		By similarity UniProtKB O74213

Sequences

	Sequence		Length	Mass (Da)
	O97400-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3059A69AF71E2A76	FASTA	367	39,426
	10 20 30 40 50 60 MSIRLIAVLS AASIAVTSAT PVADSSCTIS SFDQVASVLA ECTDIVVSNL EVPAGETLNL 70 80 90 100 110 120 ETKKKGVTIT FEGKTTFAYK EWTGPLLRVK GKAITVVGAK GSVLDGQGQL YWDGKGGNGG 130 140 150 160 170 180 IKKPKFFKIK ATEGSHFKNI NLLNCPVQVH SIDHSGPLTL SGWNIDVSQG DKDALGHNTD 190 200 210 220 230 240 GFDINTTDQL TIEDTVVKNQ DDCIAVNQGT NFLFNNLDCS GGHGLSLSVG TSHEIIKNTV 250 260 270 280 290 300 RNVTFSNSVV RKSRNGIHIK TTYQFRGRYP WRMLTYSNIA MEGIWKYAVN VEQDYKKGKP 310 320 330 340 350 360 TGIPVGNIPI KGLHLEKVTG TLTGEESTPV YIICADGACS NFNWSGVSFE GASHASNCSY VPTGYSC

References

[1]

"Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae." Girard C., Jouanin L. Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed: 10612046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Larval gut.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17906 mRNA. Translation: CAA76930.1.
3D structure databases
HSSP	HSSP built from PDB template 1NHC based on UniProtKB P26213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 290922.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 2 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_PHACE

Accession

Primary (citable) accession number: O97400
Secondary accession number(s): P81523

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)