Reviewed,
UniProtKB/Swiss-Prot O97399 (TRYP_PHACE)
Last modified
June 16, 2009.
Version 48.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Trypsin EC=3.4.21.4 |
| Organism | Phaedon cochleariae (Mustard beetle) |
| Taxonomic identifier | 80249 [NCBI] |
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Chrysomelini › Phaedon |
Protein attributes
| Sequence length | 258 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. UniProtKB P35049 |
| Subcellular location | Secreted By similarity. UniProtKB P35049 |
| Tissue specificity | Expressed in larval carcasses and gut, and adult gut. Ref.1 |
| Developmental stage | Ovarial and mature eggs, larvae and adult. Ref.1 |
| Sequence similarities | Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Digestion |
| Cellular component | Secreted |
| Domain | Signal |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Glycoprotein Zymogen |
| Gene Ontology (GO) | |
| Biological process | digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 16 | 16 | Potential | ||||||||
| Propeptide | 17 – 30 | 14 | Activation peptide By similarity UniProtKB P35049 | PRO_0000314681 | |||||||
| Chain | 30 – 258 | 229 | Trypsin UniProtKB P35049 | PRO_5000147327 | |||||||
Regions | |||||||||||
| Domain | 30 – 257 | 228 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 70 | 1 | Charge relay system By similarity UniProtKB P35049 | ||||||||
| Active site | 117 | 1 | Charge relay system By similarity UniProtKB P35049 | ||||||||
| Active site | 213 | 1 | Charge relay system By similarity UniProtKB P35049 | ||||||||
| Site | 207 | 1 | Required for specificity By similarity UniProtKB P35049 | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 110 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 130 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 188 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 55 ↔ 71 | By similarity UniProtKB P35049 | |||||||||
| Disulfide bond | 182 ↔ 197 | By similarity UniProtKB P35049 | |||||||||
| Disulfide bond | 209 ↔ 233 | By similarity UniProtKB P35049 | |||||||||
Sequences
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References
| [1] | "Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae." Girard C., Jouanin L. Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed: 10612046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Larval gut. |
Cross-references
Sequence databases | |
|---|---|
| Y17905 mRNA. Translation: CAA76929.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1EZX based on UniProtKB P00760. |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.4. 290922. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | TRYP_PHACE | ||||||||
| Accession | Primary (citable) accession number: O97399 Secondary accession number(s): P81524 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


