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O97399

- TRYP_PHACE

UniProt

O97399 - TRYP_PHACE

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Protein
Trypsin
Gene
N/A
Organism
Phaedon cochleariae (Mustard beetle)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Charge relay system By similarityBy similarity
Active sitei117 – 1171Charge relay system By similarityBy similarity
Sitei207 – 2071Required for specificity By similarityBy similarity
Active sitei213 – 2131Charge relay system By similarityBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
OrganismiPhaedon cochleariae (Mustard beetle)
Taxonomic identifieri80249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeChrysomeliniPhaedon

Subcellular locationi

Secreted By similarity By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed prediction
Add
BLAST
Propeptidei17 – 2913Activation peptide By similarity
PRO_0000314681Add
BLAST
Chaini30 – 258229TrypsinBy similarity
PRO_5000147327Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 71 By similarityBy similarity
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi130 – 1301N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi182 ↔ 197 By similarityBy similarity
Glycosylationi188 – 1881N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi209 ↔ 233 By similarityBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed in larval carcasses and gut, and adult gut.1 Publication

Developmental stagei

Ovarial and mature eggs, larvae and adult.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO97399.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 257228Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97399-1 [UniParc]FASTAAdd to Basket

« Hide

MIRFTLALAV IGVTFAASTP QIETNPNLEI IGGHDANIID YPWQISFQHR    50
LHHFCGGFLI SDTWVVTAAH CIYEGYSDTE NLNIRVGSSE WSAKGKLHDV 100
KRYITHPQYN ITTMDNDIAL LELALPVDLN QSVRPAKLPV AGQEIPDNAQ 150
LTITGWGATY VGGYNEYTLQ VVTIPTVNIN VCQSAITNDT ITNNMFCAGL 200
IGVGGKDSCS GDSGGPAVID GQVVGIVSWG YSCADPKYPG IYTKVSAFRD 250
WINEETEI 258
Length:258
Mass (Da):28,071
Last modified:May 1, 1999 - v1
Checksum:iBDBDFAFECB86866C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17905 mRNA. Translation: CAA76929.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y17905 mRNA. Translation: CAA76929.1 .

3D structure databases

ProteinModelPortali O97399.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.130.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae."
    Girard C., Jouanin L.
    Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Larval gut.

Entry informationi

Entry nameiTRYP_PHACE
AccessioniPrimary (citable) accession number: O97399
Secondary accession number(s): P81524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3