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Protein

Trypsin

Gene
N/A
Organism
Phaedon cochleariae (Mustard beetle)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Charge relay systemBy similarity
Active sitei117 – 1171Charge relay systemBy similarity
Sitei207 – 2071Required for specificityBy similarity
Active sitei213 – 2131Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
OrganismiPhaedon cochleariae (Mustard beetle)
Taxonomic identifieri80249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeChrysomeliniPhaedon

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Propeptidei17 – 2913Activation peptideBy similarityPRO_0000314681Add
BLAST
Chaini30 – 258229TrypsinSequence AnalysisBy similarityPRO_5000147327Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 71PROSITE-ProRule annotationBy similarity
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi182 ↔ 197PROSITE-ProRule annotationBy similarity
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi209 ↔ 233PROSITE-ProRule annotationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed in larval carcasses and gut, and adult gut.1 Publication

Developmental stagei

Ovarial and mature eggs, larvae and adult.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO97399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 257228Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRFTLALAV IGVTFAASTP QIETNPNLEI IGGHDANIID YPWQISFQHR
60 70 80 90 100
LHHFCGGFLI SDTWVVTAAH CIYEGYSDTE NLNIRVGSSE WSAKGKLHDV
110 120 130 140 150
KRYITHPQYN ITTMDNDIAL LELALPVDLN QSVRPAKLPV AGQEIPDNAQ
160 170 180 190 200
LTITGWGATY VGGYNEYTLQ VVTIPTVNIN VCQSAITNDT ITNNMFCAGL
210 220 230 240 250
IGVGGKDSCS GDSGGPAVID GQVVGIVSWG YSCADPKYPG IYTKVSAFRD

WINEETEI
Length:258
Mass (Da):28,071
Last modified:May 1, 1999 - v1
Checksum:iBDBDFAFECB86866C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17905 mRNA. Translation: CAA76929.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17905 mRNA. Translation: CAA76929.1.

3D structure databases

ProteinModelPortaliO97399.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae."
    Girard C., Jouanin L.
    Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Larval gut1 Publication.

Entry informationi

Entry nameiTRYP_PHACE
AccessioniPrimary (citable) accession number: O97399
Secondary accession number(s): P81524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.