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O97399

- TRYP_PHACE

UniProt

O97399 - TRYP_PHACE

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Protein

Trypsin

Gene
N/A
Organism
Phaedon cochleariae (Mustard beetle)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Charge relay systemBy similarity
Active sitei117 – 1171Charge relay systemBy similarity
Sitei207 – 2071Required for specificityBy similarity
Active sitei213 – 2131Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.130.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin (EC:3.4.21.4)
OrganismiPhaedon cochleariae (Mustard beetle)
Taxonomic identifieri80249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeChrysomeliniPhaedon

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Propeptidei17 – 2913Activation peptideBy similarityPRO_0000314681Add
BLAST
Chaini30 – 258229TrypsinBy similaritySequence AnalysisPRO_5000147327Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 71By similarityPROSITE-ProRule annotation
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi182 ↔ 197By similarityPROSITE-ProRule annotation
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi209 ↔ 233By similarityPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed in larval carcasses and gut, and adult gut.1 Publication

Developmental stagei

Ovarial and mature eggs, larvae and adult.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO97399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 257228Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97399-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIRFTLALAV IGVTFAASTP QIETNPNLEI IGGHDANIID YPWQISFQHR
60 70 80 90 100
LHHFCGGFLI SDTWVVTAAH CIYEGYSDTE NLNIRVGSSE WSAKGKLHDV
110 120 130 140 150
KRYITHPQYN ITTMDNDIAL LELALPVDLN QSVRPAKLPV AGQEIPDNAQ
160 170 180 190 200
LTITGWGATY VGGYNEYTLQ VVTIPTVNIN VCQSAITNDT ITNNMFCAGL
210 220 230 240 250
IGVGGKDSCS GDSGGPAVID GQVVGIVSWG YSCADPKYPG IYTKVSAFRD

WINEETEI
Length:258
Mass (Da):28,071
Last modified:May 1, 1999 - v1
Checksum:iBDBDFAFECB86866C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17905 mRNA. Translation: CAA76929.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17905 mRNA. Translation: CAA76929.1 .

3D structure databases

ProteinModelPortali O97399.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.130.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae."
    Girard C., Jouanin L.
    Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Larval gut1 Publication.

Entry informationi

Entry nameiTRYP_PHACE
AccessioniPrimary (citable) accession number: O97399
Secondary accession number(s): P81524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3