Reviewed, UniProtKB/Swiss-Prot O97399 (TRYP_PHACE)

Names and origin

Protein names	Recommended name:     Trypsin     EC=3.4.21.4
Organism	Phaedon cochleariae (Mustard beetle)
Taxonomic identifier	80249 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Chrysomelini › Phaedon

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa. UniProtKB P35049
Subcellular location	Secreted By similarity. UniProtKB P35049
Tissue specificity	Expressed in larval carcasses and gut, and adult gut. Ref.1
Developmental stage	Ovarial and mature eggs, larvae and adult. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 30	14	Activation peptide By similarity UniProtKB P35049		PRO_0000314681
	Chain	30 – 258	229	Trypsin UniProtKB P35049		PRO_5000147327
Regions
	Domain	30 – 257	228	Peptidase S1
Sites
	Active site	70	1	Charge relay system By similarity UniProtKB P35049
	Active site	117	1	Charge relay system By similarity UniProtKB P35049
	Active site	213	1	Charge relay system By similarity UniProtKB P35049
	Site	207	1	Required for specificity By similarity UniProtKB P35049
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Disulfide bond	55 ↔ 71		By similarity UniProtKB P35049
	Disulfide bond	182 ↔ 197		By similarity UniProtKB P35049
	Disulfide bond	209 ↔ 233		By similarity UniProtKB P35049

Sequences

	Sequence		Length	Mass (Da)
	O97399-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BDBDFAFECB86866C	FASTA	258	28,071
	10 20 30 40 50 60 MIRFTLALAV IGVTFAASTP QIETNPNLEI IGGHDANIID YPWQISFQHR LHHFCGGFLI 70 80 90 100 110 120 SDTWVVTAAH CIYEGYSDTE NLNIRVGSSE WSAKGKLHDV KRYITHPQYN ITTMDNDIAL 130 140 150 160 170 180 LELALPVDLN QSVRPAKLPV AGQEIPDNAQ LTITGWGATY VGGYNEYTLQ VVTIPTVNIN 190 200 210 220 230 240 VCQSAITNDT ITNNMFCAGL IGVGGKDSCS GDSGGPAVID GQVVGIVSWG YSCADPKYPG 250 IYTKVSAFRD WINEETEI

References

[1]

"Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae." Girard C., Jouanin L. Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed: 10612046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Larval gut.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17905 mRNA. Translation: CAA76929.1.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.4. 290922.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYP_PHACE

Accession

Primary (citable) accession number: O97399
Secondary accession number(s): P81524

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)