Reviewed, UniProtKB/Swiss-Prot O97398 (CTR_PHACE)

Names and origin

Protein names	Recommended name:     Chymotrypsin     EC=3.4.21.1
Organism	Phaedon cochleariae (Mustard beetle)
Taxonomic identifier	80249 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Chrysomelini › Phaedon

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Serine protease with chymotryptic and collagenolytic activities By similarity. UniProtKB Q00871
Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa. UniProtKB Q00871
Subcellular location	Secreted › extracellular space By similarity. UniProtKB Q00871
Tissue specificity	Expressed in larval carcasses and gut, and adult gut. Ref.1
Developmental stage	Ovarial and mature eggs, larvae and adult. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Collagen degradation Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 45	29	Activation peptide By similarity UniProtKB Q00871		PRO_0000314680
	Chain	46 – 276	231	Chymotrypsin		PRO_5000147324
Regions
	Domain	46 – 272	227	Peptidase S1
Sites
	Active site	89	1	Charge relay system By similarity UniProtKB P00771
	Active site	135	1	Charge relay system By similarity UniProtKB P00771
	Active site	229	1	Charge relay system By similarity UniProtKB P00771
Amino acid modifications
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Disulfide bond	74 ↔ 90		By similarity UniProtKB P00771
	Disulfide bond	202 ↔ 215		By similarity UniProtKB P00771
	Disulfide bond	225 ↔ 250		By similarity UniProtKB P00771

Sequences

	Sequence		Length	Mass (Da)
	O97398-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: FC5FD05DB882A1DE	FASTA	276	29,868
	10 20 30 40 50 60 MKVALVVLAL FGVSLAASID NIEIPPSKNI YVEPINQPEV DPSLEIVNGQ EVVPHSIPYQ 70 80 90 100 110 120 IFLVASAGET SWTCGGSLIT KRYVLTAAHC IQGAKSVHVT LGAHNLAKHE ASKVTVNGRS 130 140 150 160 170 180 WVIHEKYDST NIDNDIGVIQ LERNLTLTRS IQLARLPSLR DVGINLEGRT ATVSGWGLTN 190 200 210 220 230 240 GIFQTTTDVL RANNTIISNK ECNDVFKIVQ PTEVCLSIAG GRSACSGDSG GPLVIDNVQH 250 260 270 GIVSYGSSYC RSTPSVFTRV SSYLNWLQTH SEWRAQ

References

[1]

"Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae." Girard C., Jouanin L. Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed: 10612046] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Larval gut.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y17904 mRNA. Translation: CAA76928.1.
3D structure databases
HSSP	HSSP built from PDB template 1NN6 based on UniProtKB P23946.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.1. 290922.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTR_PHACE

Accession

Primary (citable) accession number: O97398
Secondary accession number(s): P81521

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)