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O97398

- CTR_PHACE

UniProt

O97398 - CTR_PHACE

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Protein

Chymotrypsin

Gene
N/A
Organism
Phaedon cochleariae (Mustard beetle)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Serine protease with chymotryptic and collagenolytic activities.By similarity

Catalytic activityi

Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.By similarityPROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Charge relay systemBy similarity
Active sitei135 – 1351Charge relay systemBy similarity
Active sitei229 – 2291Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. collagen catabolic process Source: UniProtKB-KW
  2. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Collagen degradation, Digestion

Protein family/group databases

MEROPSiS01.082.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin (EC:3.4.21.1)
OrganismiPhaedon cochleariae (Mustard beetle)
Taxonomic identifieri80249 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaColeopteraPolyphagaCucujiformiaChrysomeloideaChrysomelidaeChrysomelinaeChrysomeliniPhaedon

Subcellular locationi

Secretedextracellular space By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Propeptidei17 – 4529Activation peptideBy similarityPRO_0000314680Add
BLAST
Chaini46 – 276231ChymotrypsinSequence AnalysisPRO_5000147324Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi74 ↔ 90By similarityPROSITE-ProRule annotation
Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi202 ↔ 215By similarityPROSITE-ProRule annotation
Disulfide bondi225 ↔ 250By similarityPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed in larval carcasses and gut, and adult gut.1 Publication

Developmental stagei

Ovarial and mature eggs, larvae and adult.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO97398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 272227Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O97398-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVALVVLAL FGVSLAASID NIEIPPSKNI YVEPINQPEV DPSLEIVNGQ
60 70 80 90 100
EVVPHSIPYQ IFLVASAGET SWTCGGSLIT KRYVLTAAHC IQGAKSVHVT
110 120 130 140 150
LGAHNLAKHE ASKVTVNGRS WVIHEKYDST NIDNDIGVIQ LERNLTLTRS
160 170 180 190 200
IQLARLPSLR DVGINLEGRT ATVSGWGLTN GIFQTTTDVL RANNTIISNK
210 220 230 240 250
ECNDVFKIVQ PTEVCLSIAG GRSACSGDSG GPLVIDNVQH GIVSYGSSYC
260 270
RSTPSVFTRV SSYLNWLQTH SEWRAQ
Length:276
Mass (Da):29,868
Last modified:May 1, 1999 - v1
Checksum:iFC5FD05DB882A1DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17904 mRNA. Translation: CAA76928.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17904 mRNA. Translation: CAA76928.1 .

3D structure databases

ProteinModelPortali O97398.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.082.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae."
    Girard C., Jouanin L.
    Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Larval gut1 Publication.

Entry informationi

Entry nameiCTR_PHACE
AccessioniPrimary (citable) accession number: O97398
Secondary accession number(s): P81521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3