O97397 (CATLL_PHACE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cathepsin L-like proteinase EC=3.4.22.-
Organism	Phaedon cochleariae (Mustard beetle)
Taxonomic identifier	80249 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Chrysomeloidea › Chrysomelidae › Chrysomelinae › Chrysomelini › Phaedon

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Tissue specificity	Expressed in larval carcasses and gut, and adult gut. Ref.1
Developmental stage	Larvae and adult, but not eggs. Ref.1
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Biological process	Digestion
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – ?		Activation peptide Potential		PRO_0000223699
	Chain	? – 324		Cathepsin L-like proteinase		PRO_0000223700
Sites
	Active site	134	1	By similarity
	Active site	270	1	By similarity
	Active site	290	1	By similarity
Amino acid modifications
	Disulfide bond	131 ↔ 174		By similarity
	Disulfide bond	165 ↔ 206		By similarity
	Disulfide bond	263 ↔ 312		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O97397 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 5E0F191D2C84067F	FASTA	324	35,468
	10 20 30 40 50 60 MKLIIALAAL IVVINAASDQ ELWADFKKTH ARTYKSLREE KLRFNIFQDT LRQIAEHNVK 70 80 90 100 110 120 YENGESTYYL AINKFSDITD EEFRDMLMKN EASRPNLEGL EVADLTVGAA PESIDWRSKG 130 140 150 160 170 180 VVLPVRNQGE CGSCWALSTA AAIESQSAIK SGSKVPLSPQ QLVDCSTSYG NHGCNGGFAV 190 200 210 220 230 240 NGFEYVKDNG LESDADYPYS GKEDKCKAND KSRSVVELTG YKKVTASETS LKEAVGTIGP 250 260 270 280 290 300 ISAVVFGKPM KSYGGGIFDD SSCLGDNLHH GVNVVGYGIE NGQKYWIIKN TWGADWGESG 310 320 YIRLIRDTDH SCGVEKMASY PILA

References

[1]

"Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae." Girard C., Jouanin L. Insect Biochem. Mol. Biol. 29:1129-1142(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Larval gut.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y17903 mRNA. Translation: CAA76927.1.
3D structure databases
ProteinModelPortal	O97397.
ModBase	Search...
Protein family/group databases
MEROPS	C01.143.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATLL_PHACE

Accession

Primary (citable) accession number: O97397
Secondary accession number(s): P81520

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	May 1, 1999
Last modified:	October 3, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)