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Protein

G-protein coupled receptor Mth

Gene

mth

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in biological aging and stress response. Essential for adult survival. Required in the presynaptic motor neuron to up-regulate neurotransmitter exocytosis at larval glutamatergic neuromuscular junctions (NMJs). Regulates a step associated with docking and clustering of vesicles at release sites. SP/Acp70A and sun are agonists that activate mth in vitro.4 Publications

Miscellaneous

Agonists of mth share minimal sequence homology, suggesting a remarkable promiscuity of mth for activation.1 Publication

GO - Molecular functioni

  • G-protein coupled peptide receptor activity Source: FlyBase
  • G-protein coupled receptor activity Source: FlyBase
  • peptide binding Source: FlyBase

GO - Biological processi

  • aging Source: FlyBase
  • cell surface receptor signaling pathway Source: InterPro
  • determination of adult lifespan Source: FlyBase
  • G-protein coupled receptor signaling pathway Source: FlyBase
  • response to heat Source: UniProtKB
  • response to oxidative stress Source: FlyBase
  • response to paraquat Source: FlyBase
  • response to reactive oxygen species Source: UniProtKB
  • response to starvation Source: UniProtKB
  • synaptic vesicle exocytosis Source: FlyBase

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processExocytosis, Neurotransmitter transport, Transport

Protein family/group databases

TCDBi9.A.14.14.2 the g-protein-coupled receptor (gpcr) family

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor Mth
Alternative name(s):
Protein methuselah
Gene namesi
Name:mth
ORF Names:CG6936
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0023000 mth

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 218ExtracellularSequence analysisAdd BLAST194
Transmembranei219 – 239Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini240 – 248CytoplasmicSequence analysis9
Transmembranei249 – 269Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini270 – 278ExtracellularSequence analysis9
Transmembranei279 – 299Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini300 – 320CytoplasmicSequence analysisAdd BLAST21
Transmembranei321 – 341Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini342 – 370ExtracellularSequence analysisAdd BLAST29
Transmembranei371 – 391Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini392 – 424CytoplasmicSequence analysisAdd BLAST33
Transmembranei425 – 445Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini446 – 454ExtracellularSequence analysis9
Transmembranei455 – 475Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini476 – 514CytoplasmicSequence analysisAdd BLAST39

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Increase in average life-span and enhanced resistance to various forms of stress, including starvation, high temperature and dietary paraquat, a free-radical generator.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Add BLAST24
ChainiPRO_000001302025 – 514G-protein coupled receptor MthAdd BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi29 ↔ 83Combined sources2 Publications
Glycosylationi45N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Disulfide bondi85 ↔ 90Combined sources2 Publications
Disulfide bondi94 ↔ 188Combined sources2 Publications
Disulfide bondi95 ↔ 106Combined sources2 Publications
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi123N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Disulfide bondi150 ↔ 209Combined sources2 Publications
Glycosylationi170N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Glycosylationi198N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi449N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO97148
PRIDEiO97148

Expressioni

Gene expression databases

BgeeiFBgn0023000
ExpressionAtlasiO97148 baseline and differential
GenevisibleiO97148 DM

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi63614, 1 interactor
STRINGi7227.FBpp0072470

Structurei

Secondary structure

1514
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 33Combined sources3
Beta strandi40 – 42Combined sources3
Beta strandi48 – 50Combined sources3
Beta strandi53 – 55Combined sources3
Helixi57 – 59Combined sources3
Beta strandi60 – 67Combined sources8
Beta strandi73 – 82Combined sources10
Helixi84 – 86Combined sources3
Beta strandi90 – 95Combined sources6
Beta strandi100 – 102Combined sources3
Beta strandi105 – 107Combined sources3
Helixi112 – 117Combined sources6
Beta strandi121 – 125Combined sources5
Beta strandi131 – 135Combined sources5
Turni136 – 139Combined sources4
Beta strandi140 – 143Combined sources4
Helixi161 – 163Combined sources3
Beta strandi165 – 168Combined sources4
Beta strandi173 – 175Combined sources3
Turni176 – 179Combined sources4
Beta strandi180 – 182Combined sources3
Beta strandi186 – 195Combined sources10
Beta strandi197 – 208Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FJRX-ray2.30A/B25-219[»]
2PZXX-ray3.50A/B/C/D25-212[»]
ProteinModelPortaliO97148
SMRiO97148
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO97148

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193 Eukaryota
ENOG410XSD2 LUCA
GeneTreeiENSGT00530000064566
InParanoidiO97148
KOiK04599
OMAiGFYNCWI
OrthoDBiEOG091G04T4
PhylomeDBiO97148

Family and domain databases

CDDicd00251 Mth_Ecto, 1 hit
Gene3Di2.170.180.11, 1 hit
InterProiView protein in InterPro
IPR017981 GPCR_2-like
IPR000832 GPCR_2_secretin-like
IPR023311 Methusela_ecto_dom_2
IPR010596 Methuselah_N_dom
IPR036272 Methuselah_N_sf
PfamiView protein in Pfam
PF00002 7tm_2, 1 hit
PF06652 Methuselah_N, 1 hit
SUPFAMiSSF63877 SSF63877, 1 hit
PROSITEiView protein in PROSITE
PS50261 G_PROTEIN_RECEP_F2_4, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: O97148-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTLLVLRIS TVILVVLVIQ KSYADILECD YFDTVDISAA QKLQNGSYLF
60 70 80 90 100
EGLLVPAILT GEYDFRILPD DSKQKVARHI RGCVCKLKPC VRFCCPHDHI
110 120 130 140 150
MDNGVCYDNM SDEELAELDP FLNVTLDDGS VSRRHFKNEL IVQWDLPMPC
160 170 180 190 200
DGMFYLDNRE EQDKYTLFEN GTFFRHFDRV TLRKREYCLQ HLTFADGNAT
210 220 230 240 250
SIRIAPHNCL IVPSITGQTV VMISSLICMV LTIAVYLFVK KLQNLHGKCF
260 270 280 290 300
ICYMVCLFMG YLFLLLDLWQ ISISFCKPAG FLGYFFVMAA FFWLSVISLH
310 320 330 340 350
LWNTFRGSSH KANRFLFEHR FLAYNTYAWG MAVVLTGITV LADNIVENQD
360 370 380 390 400
WNPRVGHEGH CWIYTQAWSA MLYFYGPMVF LIAFNITMFI LTAKRILGVK
410 420 430 440 450
KDIQNFAHRQ ERKQKLNSDK QTYTFFLRLF IIMGLSWSLE IGSYFSQSNQ
460 470 480 490 500
TWANVFLVAD YLNWSQGIII FILFVLKRST WRLLQESIRG EGEEVNNSEE
510
EISLENTTTR NVLL
Length:514
Mass (Da):59,644
Last modified:May 1, 1999 - v1
Checksum:iF2A5370CECCD1D5C
GO
Isoform B (identifier: O97148-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     487-514: SIRGEGEEVNNSEEEISLENTTTRNVLL → RNHPKSTRSVISNRSMASRITVGTTPKNKARNSPLA

Note: No experimental confirmation available.
Show »
Length:522
Mass (Da):60,446
Checksum:i81C85F368E891600
GO

Sequence cautioni

The sequence AAG22708 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22709 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22710 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22711 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22712 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22713 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22714 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22715 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22716 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22717 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22718 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22719 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22720 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22721 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22722 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22723 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22724 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22725 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22726 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22727 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22728 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22729 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22730 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22731 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22732 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22733 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22734 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22735 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22736 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22737 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG22738 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti54L → I in strain: MA97_6. 1 Publication1
Natural varianti132S → A in strain: ZIM(S)49 and ZIM(H)44. 1 Publication1
Natural varianti151D → N in strain: CT97_3, MFL97_1 and ZIM(S)37. 1 Publication1
Natural varianti281F → V in strain: ZIM(S)49 and ZIM(H)44. 1 Publication1
Natural varianti357H → N in strain: DPF96_3.0, HFL97_15, JFL97_1, JFL97_9 and ZIM(S)37. 1 Publication1
Natural varianti387T → N in strain: ZIM(S)35. 1 Publication1
Natural varianti407A → V in strain: ZIM(H)23. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002023487 – 514SIRGE…RNVLL → RNHPKSTRSVISNRSMASRI TVGTTPKNKARNSPLA in isoform B. 2 PublicationsAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109308 mRNA Translation: AAD16981.1
AF280552 Genomic DNA Translation: AAG22708.1 Sequence problems.
AF280553 Genomic DNA Translation: AAG22709.1 Sequence problems.
AF280554 Genomic DNA Translation: AAG22710.1 Sequence problems.
AF280555 Genomic DNA Translation: AAG22711.1 Sequence problems.
AF280556 Genomic DNA Translation: AAG22712.1 Sequence problems.
AF280557 Genomic DNA Translation: AAG22713.1 Sequence problems.
AF280558 Genomic DNA Translation: AAG22714.1 Sequence problems.
AF280559 Genomic DNA Translation: AAG22715.1 Sequence problems.
AF280560 Genomic DNA Translation: AAG22716.1 Sequence problems.
AF280561 Genomic DNA Translation: AAG22717.1 Sequence problems.
AF280562 Genomic DNA Translation: AAG22718.1 Sequence problems.
AF280563 Genomic DNA Translation: AAG22719.1 Sequence problems.
AF280564 Genomic DNA Translation: AAG22720.1 Sequence problems.
AF280565 Genomic DNA Translation: AAG22721.1 Sequence problems.
AF280566 Genomic DNA Translation: AAG22722.1 Sequence problems.
AF280567 Genomic DNA Translation: AAG22723.1 Sequence problems.
AF280568 Genomic DNA Translation: AAG22724.1 Sequence problems.
AF280569 Genomic DNA Translation: AAG22725.1 Sequence problems.
AF280570 Genomic DNA Translation: AAG22726.1 Sequence problems.
AF280571 Genomic DNA Translation: AAG22727.1 Sequence problems.
AF280572 Genomic DNA Translation: AAG22728.1 Sequence problems.
AF280573 Genomic DNA Translation: AAG22729.1 Sequence problems.
AF280574 Genomic DNA Translation: AAG22730.1 Sequence problems.
AF280575 Genomic DNA Translation: AAG22731.1 Sequence problems.
AF280576 Genomic DNA Translation: AAG22732.1 Sequence problems.
AF280577 Genomic DNA Translation: AAG22733.1 Sequence problems.
AF280578 Genomic DNA Translation: AAG22734.1 Sequence problems.
AF280579 Genomic DNA Translation: AAG22735.1 Sequence problems.
AF280580 Genomic DNA Translation: AAG22736.1 Sequence problems.
AF280581 Genomic DNA Translation: AAG22737.1 Sequence problems.
AF280582 Genomic DNA Translation: AAG22738.1 Sequence problems.
AE014296 Genomic DNA Translation: AAF47379.2
AE014296 Genomic DNA Translation: AAN11440.1
AY052111 mRNA Translation: AAK93535.1
BT044433 mRNA Translation: ACH92498.1
RefSeqiNP_001246535.1, NM_001259606.2 [O97148-1]
NP_523871.1, NM_079147.4 [O97148-1]
NP_728521.1, NM_167829.3 [O97148-2]
UniGeneiDm.4379

Genome annotation databases

EnsemblMetazoaiFBtr0072570; FBpp0072469; FBgn0023000 [O97148-1]
FBtr0072571; FBpp0072470; FBgn0023000 [O97148-2]
FBtr0306850; FBpp0297746; FBgn0023000 [O97148-1]
GeneIDi38058
KEGGidme:Dmel_CG6936
UCSCiCG6936-RA d. melanogaster [O97148-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMTH_DROME
AccessioniPrimary (citable) accession number: O97148
Secondary accession number(s): B5RJF9
, Q9GN21, Q9GN78, Q9GND6, Q9GNE8, Q9GT57, Q9GT58, Q9GT59, Q9GT60, Q9W0R4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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