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Protein

Serine/threonine-protein kinase PLK4

Gene

SAK

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as Sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATPPROSITE-ProRule annotation1
Active sitei138Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • centriole replication Source: FlyBase
  • centrosome organization Source: FlyBase
  • male meiosis Source: FlyBase
  • positive regulation of protein catabolic process Source: FlyBase
  • protein autophosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of centriole replication Source: FlyBase
  • regulation of protein stability Source: FlyBase
  • sperm axoneme assembly Source: FlyBase
  • syncytial blastoderm mitotic cell cycle Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO97143.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PLK4 (EC:2.7.11.21)
Alternative name(s):
Polo-like kinase 4
Short name:
PLK-4
Serine/threonine-protein kinase SAK
Gene namesi
Name:SAK
ORF Names:CG7186
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0026371. SAK.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: FlyBase

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi293S → A in SAK-ND; impairs interaction with SCF-slmb ubiquitin ligase complex and subsequent ubiquitination; when associated with A-297. 1 Publication1
Mutagenesisi297T → A in SAK-ND; impairs interaction with SCF-slmb ubiquitin ligase complex and subsequent ubiquitination; when associated with A-292. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003852911 – 769Serine/threonine-protein kinase PLK4Add BLAST769

Post-translational modificationi

Ubiquitinated by the SCF-slmb ubiquitin ligase complex; leading to its degradation by the proteasome during interphase and regulating centriole number and ensuring the block to centriole reduplication.2 Publications

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiO97143.
PRIDEiO97143.

Expressioni

Gene expression databases

BgeeiFBgn0026371.
GenevisibleiO97143. DM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi65629. 62 interactors.
DIPiDIP-49071N.
IntActiO97143. 55 interactors.
MINTiMINT-7952919.
STRINGi7227.FBpp0078042.

Structurei

Secondary structure

1769
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi397 – 400Combined sources4
Beta strandi402 – 408Combined sources7
Beta strandi414 – 421Combined sources8
Turni423 – 425Combined sources3
Beta strandi426 – 436Combined sources11
Beta strandi442 – 446Combined sources5
Turni449 – 452Combined sources4
Helixi468 – 470Combined sources3
Beta strandi471 – 473Combined sources3
Turni474 – 476Combined sources3
Helixi479 – 481Combined sources3
Helixi482 – 496Combined sources15
Beta strandi499 – 505Combined sources7
Beta strandi507 – 514Combined sources8
Beta strandi520 – 524Combined sources5
Beta strandi529 – 533Combined sources5
Turni534 – 536Combined sources3
Beta strandi537 – 541Combined sources5
Helixi554 – 578Combined sources25
Beta strandi589 – 593Combined sources5
Beta strandi660 – 666Combined sources7
Turni667 – 669Combined sources3
Beta strandi670 – 675Combined sources6
Beta strandi680 – 684Combined sources5
Beta strandi689 – 692Combined sources4
Helixi695 – 697Combined sources3
Beta strandi701 – 704Combined sources4
Beta strandi710 – 713Combined sources4
Helixi723 – 727Combined sources5
Helixi730 – 738Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G7NX-ray2.30A/B382-602[»]
4NK7X-ray3.23A383-601[»]
5LHXX-ray1.53A/B657-745[»]
ProteinModelPortaliO97143.
SMRiO97143.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 267Protein kinasePROSITE-ProRule annotationAdd BLAST254
Domaini665 – 734POLO boxPROSITE-ProRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0575. Eukaryota.
ENOG410XQBP. LUCA.
GeneTreeiENSGT00530000062954.
InParanoidiO97143.
KOiK08863.
OMAiKNAIMSI.
OrthoDBiEOG091G05NQ.
PhylomeDBiO97143.

Family and domain databases

CDDicd13114. POLO_box_Plk4_1. 1 hit.
cd13115. POLO_box_Plk4_2. 1 hit.
cd13116. POLO_box_Plk4_3. 1 hit.
InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR033700. Plk4.
IPR000959. POLO_box_dom.
IPR033699. POLO_box_Plk4_1.
IPR033698. POLO_box_Plk4_2.
IPR033696. POLO_box_Plk4_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008266. Tyr_kinase_AS.
PANTHERiPTHR24345:SF64. PTHR24345:SF64. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50078. POLO_BOX. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.

Sequencei

Sequence statusi: Complete.

O97143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSNRAFGET IEDYEVQHLL GKGGFATVYK ARCLHTHQDV AIKMIDKKLI
60 70 80 90 100
QGTGLTNRVR QEVEIHSRLK HPSVLQLYTF FQDANYVYLV LELAHNGELH
110 120 130 140 150
RYMNHIARPF TETEAASILK QVVAGLLYLH SHNIMHRDIS LSNLLLSREM
160 170 180 190 200
HVKIADFGLA TQLKRPDERH MTMCGTPNYI SPEVVSRTSH GLPADVWSVG
210 220 230 240 250
CMLYTLLVGR PPFETDAVQS TLNKVVMSEY IMPAHLSYEA QDLINKLLKK
260 270 280 290 300
LPHERITLEA VLCHPFMLKC SNGGHSAPGA LNVFSQSMES GDSGIITFAS
310 320 330 340 350
SDSRNSQQIR SVENSGPQQV LPQIREEFKQ VHHKLPYEQT GLFGQASTGL
360 370 380 390 400
AEPNWPGAAK SSAFCMEAGN VPNSKQASLK EDRISVPPLN TKRLLPTRYK
410 420 430 440 450
TKNAIMSILR NGEVVLEFLK FRPTYNEDRI NDICRISDDG QRIIIYQPDP
460 470 480 490 500
GRGLPVREQP PDLQIPSGDC VYNYDNLPSK HWKKYIYGAR FVGLVKSKTP
510 520 530 540 550
KVTYFSTLGK CQLMETMTDF EIRFYSGAKL LKTPSEGLKV YDRNGMLLSD
560 570 580 590 600
YSCSESRSLI EHGNECFTHC VNISNALEVA QTKDNSCFPV TIGRRPITDV
610 620 630 640 650
QPAQRLDGLR DTTNIAFSTP KSNQGSINFS LSTISSTRNT SDFGTNCSRS
660 670 680 690 700
NMLAAHQNIP IKRINVPEIG IATELSHGVV QVQFYDGSVV SVIPSMQGGG
710 720 730 740 750
ITYTQPNGTS THFGKGDDLP FPVRDRVGQI PNIQLKLKTA PLLGSGRKTD
760
YNNAMTPKTT TPYYNRMLL
Length:769
Mass (Da):85,887
Last modified:May 1, 1999 - v1
Checksum:iF050BF60A5D94AA4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145L → V in AAO45202 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106952 mRNA. Translation: AAD19607.1.
AE014296 Genomic DNA. Translation: AAF51737.1.
BT004846 mRNA. Translation: AAO45202.1.
BT044561 mRNA. Translation: ACI15756.1.
RefSeqiNP_649324.1. NM_141067.3.
UniGeneiDm.3240.

Genome annotation databases

EnsemblMetazoaiFBtr0078387; FBpp0078042; FBgn0026371.
GeneIDi40384.
KEGGidme:Dmel_CG7186.
UCSCiCG7186-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106952 mRNA. Translation: AAD19607.1.
AE014296 Genomic DNA. Translation: AAF51737.1.
BT004846 mRNA. Translation: AAO45202.1.
BT044561 mRNA. Translation: ACI15756.1.
RefSeqiNP_649324.1. NM_141067.3.
UniGeneiDm.3240.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G7NX-ray2.30A/B382-602[»]
4NK7X-ray3.23A383-601[»]
5LHXX-ray1.53A/B657-745[»]
ProteinModelPortaliO97143.
SMRiO97143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65629. 62 interactors.
DIPiDIP-49071N.
IntActiO97143. 55 interactors.
MINTiMINT-7952919.
STRINGi7227.FBpp0078042.

Proteomic databases

PaxDbiO97143.
PRIDEiO97143.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078387; FBpp0078042; FBgn0026371.
GeneIDi40384.
KEGGidme:Dmel_CG7186.
UCSCiCG7186-RA. d. melanogaster.

Organism-specific databases

CTDi40384.
FlyBaseiFBgn0026371. SAK.

Phylogenomic databases

eggNOGiKOG0575. Eukaryota.
ENOG410XQBP. LUCA.
GeneTreeiENSGT00530000062954.
InParanoidiO97143.
KOiK08863.
OMAiKNAIMSI.
OrthoDBiEOG091G05NQ.
PhylomeDBiO97143.

Enzyme and pathway databases

SignaLinkiO97143.

Miscellaneous databases

GenomeRNAii40384.
PROiPR:O97143.

Gene expression databases

BgeeiFBgn0026371.
GenevisibleiO97143. DM.

Family and domain databases

CDDicd13114. POLO_box_Plk4_1. 1 hit.
cd13115. POLO_box_Plk4_2. 1 hit.
cd13116. POLO_box_Plk4_3. 1 hit.
InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR033700. Plk4.
IPR000959. POLO_box_dom.
IPR033699. POLO_box_Plk4_1.
IPR033698. POLO_box_Plk4_2.
IPR033696. POLO_box_Plk4_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008266. Tyr_kinase_AS.
PANTHERiPTHR24345:SF64. PTHR24345:SF64. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50078. POLO_BOX. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLK4_DROME
AccessioniPrimary (citable) accession number: O97143
Secondary accession number(s): Q86NL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1999
Last modified: May 10, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.