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Protein

Serine/threonine-protein kinase PLK4

Gene

SAK

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as Sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATPPROSITE-ProRule annotation1
Active sitei138Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • centriole replication Source: FlyBase
  • centrosome cycle Source: FlyBase
  • male meiotic nuclear division Source: FlyBase
  • positive regulation of protein catabolic process Source: FlyBase
  • protein autophosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of centriole replication Source: FlyBase
  • regulation of protein stability Source: FlyBase
  • sperm axoneme assembly Source: FlyBase
  • syncytial blastoderm mitotic cell cycle Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO97143

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PLK4 (EC:2.7.11.21)
Alternative name(s):
Polo-like kinase 4
Short name:
PLK-4
Serine/threonine-protein kinase SAK
Gene namesi
Name:SAK
ORF Names:CG7186
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0026371 SAK

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi293S → A in SAK-ND; impairs interaction with SCF-slmb ubiquitin ligase complex and subsequent ubiquitination; when associated with A-297. 1 Publication1
Mutagenesisi297T → A in SAK-ND; impairs interaction with SCF-slmb ubiquitin ligase complex and subsequent ubiquitination; when associated with A-292. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003852911 – 769Serine/threonine-protein kinase PLK4Add BLAST769

Post-translational modificationi

Ubiquitinated by the SCF-slmb ubiquitin ligase complex; leading to its degradation by the proteasome during interphase and regulating centriole number and ensuring the block to centriole reduplication.2 Publications

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiO97143
PRIDEiO97143

PTM databases

iPTMnetiO97143

Expressioni

Gene expression databases

BgeeiFBgn0026371
GenevisibleiO97143 DM

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi65629, 73 interactors
DIPiDIP-49071N
ELMiO97143
IntActiO97143, 65 interactors
STRINGi7227.FBpp0078042

Structurei

Secondary structure

1769
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi397 – 400Combined sources4
Beta strandi402 – 408Combined sources7
Beta strandi414 – 421Combined sources8
Turni423 – 425Combined sources3
Beta strandi426 – 436Combined sources11
Beta strandi442 – 446Combined sources5
Turni449 – 452Combined sources4
Helixi468 – 470Combined sources3
Beta strandi471 – 473Combined sources3
Turni474 – 476Combined sources3
Helixi479 – 481Combined sources3
Helixi482 – 496Combined sources15
Beta strandi499 – 505Combined sources7
Beta strandi507 – 514Combined sources8
Beta strandi520 – 524Combined sources5
Beta strandi529 – 533Combined sources5
Turni534 – 536Combined sources3
Beta strandi537 – 541Combined sources5
Helixi554 – 578Combined sources25
Beta strandi589 – 593Combined sources5
Beta strandi660 – 666Combined sources7
Turni667 – 669Combined sources3
Beta strandi670 – 675Combined sources6
Beta strandi680 – 684Combined sources5
Beta strandi689 – 692Combined sources4
Helixi695 – 697Combined sources3
Beta strandi701 – 704Combined sources4
Beta strandi710 – 713Combined sources4
Helixi723 – 727Combined sources5
Helixi730 – 738Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4G7NX-ray2.30A/B382-602[»]
4NK7X-ray3.23A383-601[»]
5LHXX-ray1.53A/B657-745[»]
ProteinModelPortaliO97143
SMRiO97143
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 267Protein kinasePROSITE-ProRule annotationAdd BLAST254
Domaini665 – 734POLO boxPROSITE-ProRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0575 Eukaryota
ENOG410XQBP LUCA
GeneTreeiENSGT00530000062954
InParanoidiO97143
KOiK08863
OMAiGADFEVW
OrthoDBiEOG091G05NQ
PhylomeDBiO97143

Family and domain databases

CDDicd13114 POLO_box_Plk4_1, 1 hit
cd13115 POLO_box_Plk4_2, 1 hit
cd13116 POLO_box_Plk4_3, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR033700 Plk4
IPR000959 POLO_box_dom
IPR033699 POLO_box_Plk4_1
IPR033698 POLO_box_Plk4_2
IPR033696 POLO_box_Plk4_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008266 Tyr_kinase_AS
PANTHERiPTHR24345:SF50 PTHR24345:SF50, 2 hits
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50078 POLO_BOX, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

O97143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSNRAFGET IEDYEVQHLL GKGGFATVYK ARCLHTHQDV AIKMIDKKLI
60 70 80 90 100
QGTGLTNRVR QEVEIHSRLK HPSVLQLYTF FQDANYVYLV LELAHNGELH
110 120 130 140 150
RYMNHIARPF TETEAASILK QVVAGLLYLH SHNIMHRDIS LSNLLLSREM
160 170 180 190 200
HVKIADFGLA TQLKRPDERH MTMCGTPNYI SPEVVSRTSH GLPADVWSVG
210 220 230 240 250
CMLYTLLVGR PPFETDAVQS TLNKVVMSEY IMPAHLSYEA QDLINKLLKK
260 270 280 290 300
LPHERITLEA VLCHPFMLKC SNGGHSAPGA LNVFSQSMES GDSGIITFAS
310 320 330 340 350
SDSRNSQQIR SVENSGPQQV LPQIREEFKQ VHHKLPYEQT GLFGQASTGL
360 370 380 390 400
AEPNWPGAAK SSAFCMEAGN VPNSKQASLK EDRISVPPLN TKRLLPTRYK
410 420 430 440 450
TKNAIMSILR NGEVVLEFLK FRPTYNEDRI NDICRISDDG QRIIIYQPDP
460 470 480 490 500
GRGLPVREQP PDLQIPSGDC VYNYDNLPSK HWKKYIYGAR FVGLVKSKTP
510 520 530 540 550
KVTYFSTLGK CQLMETMTDF EIRFYSGAKL LKTPSEGLKV YDRNGMLLSD
560 570 580 590 600
YSCSESRSLI EHGNECFTHC VNISNALEVA QTKDNSCFPV TIGRRPITDV
610 620 630 640 650
QPAQRLDGLR DTTNIAFSTP KSNQGSINFS LSTISSTRNT SDFGTNCSRS
660 670 680 690 700
NMLAAHQNIP IKRINVPEIG IATELSHGVV QVQFYDGSVV SVIPSMQGGG
710 720 730 740 750
ITYTQPNGTS THFGKGDDLP FPVRDRVGQI PNIQLKLKTA PLLGSGRKTD
760
YNNAMTPKTT TPYYNRMLL
Length:769
Mass (Da):85,887
Last modified:May 1, 1999 - v1
Checksum:iF050BF60A5D94AA4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145L → V in AAO45202 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106952 mRNA Translation: AAD19607.1
AE014296 Genomic DNA Translation: AAF51737.1
BT004846 mRNA Translation: AAO45202.1
BT044561 mRNA Translation: ACI15756.1
RefSeqiNP_649324.1, NM_141067.3
UniGeneiDm.3240

Genome annotation databases

EnsemblMetazoaiFBtr0078387; FBpp0078042; FBgn0026371
GeneIDi40384
KEGGidme:Dmel_CG7186
UCSCiCG7186-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiPLK4_DROME
AccessioniPrimary (citable) accession number: O97143
Secondary accession number(s): Q86NL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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