Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O97069

- ASSY_DROME

UniProt

O97069 - ASSY_DROME

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Argininosuccinate synthase

Gene

CG1315

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei86 – 861CitrullineBy similarity
Binding sitei91 – 911CitrullineBy similarity
Binding sitei118 – 1181AspartateBy similarity
Binding sitei122 – 1221AspartateBy similarity
Binding sitei122 – 1221CitrullineBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei126 – 1261CitrullineBy similarity
Binding sitei179 – 1791CitrullineBy similarity
Binding sitei188 – 1881CitrullineBy similarity
Binding sitei270 – 2701CitrullineBy similarity
Binding sitei282 – 2821CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 179ATPBy similarity
Nucleotide bindingi114 – 1229ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-UniPathway
  2. urea cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_210740. Urea cycle.
UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
ORF Names:CG1315
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0026565. CG1315.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Argininosuccinate synthasePRO_0000148557Add
BLAST

Proteomic databases

PRIDEiO97069.

Expressioni

Gene expression databases

BgeeiO97069.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi66010. 1 interaction.
DIPiDIP-21101N.
IntActiO97069. 1 interaction.
MINTiMINT-958605.
STRINGi7227.FBpp0081197.

Structurei

3D structure databases

ProteinModelPortaliO97069.
SMRiO97069. Positions 3-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
InParanoidiO97069.
KOiK01940.
OMAiYKALAPH.
OrthoDBiEOG7PVWPB.
PhylomeDBiO97069.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O97069 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPKETVILAY SGGLDTSCVL KWLLDKQYEV ICVLADVGQK EDFTAAEKKA
60 70 80 90 100
LKIGAKKVIV ADVKQSFVED YIWPAVQMGL VYEERYLLGT SLARPCISVA
110 120 130 140 150
LMEVAREYGA KYLAHGATGK GNDQVRFELC AYALKPDLKI IAPWRDVEFC
160 170 180 190 200
CQFQGRQDLI AYAQQHGIEV SAKPATPWST DANILHISYE SGILEDPNTV
210 220 230 240 250
APENLYEMTV DPLTRAPRDP VHLVIQFDRG LPSSVEDLPG GRVYTKPLEM
260 270 280 290 300
LDFLNKLGGS YGIGRIDIVE NRFVGLKSRG VYETPGGTIL FAAHQDLEVF
310 320 330 340 350
ALDREVLRTK QVLRDRMADY VYNGFWFSPE AIYARKCIEL AEQRVSGKVT
360 370 380 390 400
VELAPGYCRA IARKAAKDVG ALYNEQLVSM DVHGGYVPQD AGGFIAINAV
410
RIREHVRAFG AYDVPTKKN
Length:419
Mass (Da):46,595
Last modified:May 1, 1999 - v1
Checksum:i0566A10A329BE513
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001572 Genomic DNA. Translation: AAD19816.1.
AE014297 Genomic DNA. Translation: AAF54103.2.
BT060440 mRNA. Translation: ACN22205.1.
RefSeqiNP_001262324.1. NM_001275395.1.
NP_649674.1. NM_141417.3.
UniGeneiDm.20149.

Genome annotation databases

EnsemblMetazoaiFBtr0081699; FBpp0081197; FBgn0026565.
GeneIDi40812.
KEGGidme:Dmel_CG1315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE001572 Genomic DNA. Translation: AAD19816.1 .
AE014297 Genomic DNA. Translation: AAF54103.2 .
BT060440 mRNA. Translation: ACN22205.1 .
RefSeqi NP_001262324.1. NM_001275395.1.
NP_649674.1. NM_141417.3.
UniGenei Dm.20149.

3D structure databases

ProteinModelPortali O97069.
SMRi O97069. Positions 3-407.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 66010. 1 interaction.
DIPi DIP-21101N.
IntActi O97069. 1 interaction.
MINTi MINT-958605.
STRINGi 7227.FBpp0081197.

Proteomic databases

PRIDEi O97069.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0081699 ; FBpp0081197 ; FBgn0026565 .
GeneIDi 40812.
KEGGi dme:Dmel_CG1315.

Organism-specific databases

FlyBasei FBgn0026565. CG1315.

Phylogenomic databases

eggNOGi COG0137.
GeneTreei ENSGT00390000004524.
InParanoidi O97069.
KOi K01940.
OMAi YKALAPH.
OrthoDBi EOG7PVWPB.
PhylomeDBi O97069.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
UPA00158 ; UER00272 .
Reactomei REACT_210740. Urea cycle.

Miscellaneous databases

GenomeRNAii 40812.
NextBioi 820710.
PROi O97069.

Gene expression databases

Bgeei O97069.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the Antennapedia complex of Drosophila."
    Celniker S.E., Pfeiffer B., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Berkeley.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Carlson J.W., Booth B., Frise E., Sandler J., Wan K.H., Yu C., Celniker S.E.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.

Entry informationi

Entry nameiASSY_DROME
AccessioniPrimary (citable) accession number: O97069
Secondary accession number(s): C0HDN4, Q9VI41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: October 1, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3