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Protein

Argininosuccinate synthase

Gene

CG1315

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (CG1315)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathway:iurea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (CG1315)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei86 – 861CitrullineBy similarity
Binding sitei91 – 911CitrullineBy similarity
Binding sitei118 – 1181AspartateBy similarity
Binding sitei122 – 1221AspartateBy similarity
Binding sitei122 – 1221CitrullineBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei126 – 1261CitrullineBy similarity
Binding sitei179 – 1791CitrullineBy similarity
Binding sitei188 – 1881CitrullineBy similarity
Binding sitei270 – 2701CitrullineBy similarity
Binding sitei282 – 2821CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 179ATPBy similarity
Nucleotide bindingi114 – 1229ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_292514. Urea cycle.
UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
ORF Names:CG1315
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0026565. CG1315.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Argininosuccinate synthasePRO_0000148557Add
BLAST

Proteomic databases

PRIDEiO97069.

Expressioni

Gene expression databases

BgeeiO97069.
GenevisibleiO97069. DM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi66010. 1 interaction.
DIPiDIP-21101N.
IntActiO97069. 1 interaction.
MINTiMINT-958605.
STRINGi7227.FBpp0081197.

Structurei

3D structure databases

ProteinModelPortaliO97069.
SMRiO97069. Positions 3-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
InParanoidiO97069.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG7PVWPB.
PhylomeDBiO97069.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O97069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKETVILAY SGGLDTSCVL KWLLDKQYEV ICVLADVGQK EDFTAAEKKA
60 70 80 90 100
LKIGAKKVIV ADVKQSFVED YIWPAVQMGL VYEERYLLGT SLARPCISVA
110 120 130 140 150
LMEVAREYGA KYLAHGATGK GNDQVRFELC AYALKPDLKI IAPWRDVEFC
160 170 180 190 200
CQFQGRQDLI AYAQQHGIEV SAKPATPWST DANILHISYE SGILEDPNTV
210 220 230 240 250
APENLYEMTV DPLTRAPRDP VHLVIQFDRG LPSSVEDLPG GRVYTKPLEM
260 270 280 290 300
LDFLNKLGGS YGIGRIDIVE NRFVGLKSRG VYETPGGTIL FAAHQDLEVF
310 320 330 340 350
ALDREVLRTK QVLRDRMADY VYNGFWFSPE AIYARKCIEL AEQRVSGKVT
360 370 380 390 400
VELAPGYCRA IARKAAKDVG ALYNEQLVSM DVHGGYVPQD AGGFIAINAV
410
RIREHVRAFG AYDVPTKKN
Length:419
Mass (Da):46,595
Last modified:May 1, 1999 - v1
Checksum:i0566A10A329BE513
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001572 Genomic DNA. Translation: AAD19816.1.
AE014297 Genomic DNA. Translation: AAF54103.2.
BT060440 mRNA. Translation: ACN22205.1.
RefSeqiNP_001262324.1. NM_001275395.1.
NP_649674.1. NM_141417.3.
UniGeneiDm.20149.

Genome annotation databases

EnsemblMetazoaiFBtr0081699; FBpp0081197; FBgn0026565.
FBtr0337022; FBpp0307951; FBgn0026565.
GeneIDi40812.
KEGGidme:Dmel_CG1315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001572 Genomic DNA. Translation: AAD19816.1.
AE014297 Genomic DNA. Translation: AAF54103.2.
BT060440 mRNA. Translation: ACN22205.1.
RefSeqiNP_001262324.1. NM_001275395.1.
NP_649674.1. NM_141417.3.
UniGeneiDm.20149.

3D structure databases

ProteinModelPortaliO97069.
SMRiO97069. Positions 3-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66010. 1 interaction.
DIPiDIP-21101N.
IntActiO97069. 1 interaction.
MINTiMINT-958605.
STRINGi7227.FBpp0081197.

Proteomic databases

PRIDEiO97069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081699; FBpp0081197; FBgn0026565.
FBtr0337022; FBpp0307951; FBgn0026565.
GeneIDi40812.
KEGGidme:Dmel_CG1315.

Organism-specific databases

FlyBaseiFBgn0026565. CG1315.

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
InParanoidiO97069.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG7PVWPB.
PhylomeDBiO97069.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.
ReactomeiREACT_292514. Urea cycle.

Miscellaneous databases

GenomeRNAii40812.
NextBioi820710.
PROiO97069.

Gene expression databases

BgeeiO97069.
GenevisibleiO97069. DM.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the Antennapedia complex of Drosophila."
    Celniker S.E., Pfeiffer B., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Berkeley.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Carlson J.W., Booth B., Frise E., Sandler J., Wan K.H., Yu C., Celniker S.E.
    Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.

Entry informationi

Entry nameiASSY_DROME
AccessioniPrimary (citable) accession number: O97069
Secondary accession number(s): C0HDN4, Q9VI41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 22, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.