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Protein

Argininosuccinate synthase

Gene

CG1315

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (CG1315)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (CG1315)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei86CitrullineBy similarity1
Binding sitei91CitrullineBy similarity1
Binding sitei118AspartateBy similarity1
Binding sitei122AspartateBy similarity1
Binding sitei122CitrullineBy similarity1
Binding sitei123AspartateBy similarity1
Binding sitei126CitrullineBy similarity1
Binding sitei179CitrullineBy similarity1
Binding sitei188CitrullineBy similarity1
Binding sitei270CitrullineBy similarity1
Binding sitei282CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 17ATPBy similarity9
Nucleotide bindingi114 – 122ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Urea cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113
UPA00158; UER00272

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
ORF Names:CG1315
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0026565 CG1315

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485571 – 419Argininosuccinate synthaseAdd BLAST419

Proteomic databases

PaxDbiO97069
PRIDEiO97069

Expressioni

Gene expression databases

BgeeiFBgn0026565
ExpressionAtlasiO97069 baseline and differential
GenevisibleiO97069 DM

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi66010, 1 interactor
DIPiDIP-21101N
IntActiO97069, 1 interactor
STRINGi7227.FBpp0081197

Structurei

3D structure databases

ProteinModelPortaliO97069
SMRiO97069
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1706 Eukaryota
COG0137 LUCA
GeneTreeiENSGT00390000004524
InParanoidiO97069
KOiK01940
OMAiGVGRIDM
OrthoDBiEOG091G0AIR
PhylomeDBiO97069

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.90.1260.10, 1 hit
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

O97069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKETVILAY SGGLDTSCVL KWLLDKQYEV ICVLADVGQK EDFTAAEKKA
60 70 80 90 100
LKIGAKKVIV ADVKQSFVED YIWPAVQMGL VYEERYLLGT SLARPCISVA
110 120 130 140 150
LMEVAREYGA KYLAHGATGK GNDQVRFELC AYALKPDLKI IAPWRDVEFC
160 170 180 190 200
CQFQGRQDLI AYAQQHGIEV SAKPATPWST DANILHISYE SGILEDPNTV
210 220 230 240 250
APENLYEMTV DPLTRAPRDP VHLVIQFDRG LPSSVEDLPG GRVYTKPLEM
260 270 280 290 300
LDFLNKLGGS YGIGRIDIVE NRFVGLKSRG VYETPGGTIL FAAHQDLEVF
310 320 330 340 350
ALDREVLRTK QVLRDRMADY VYNGFWFSPE AIYARKCIEL AEQRVSGKVT
360 370 380 390 400
VELAPGYCRA IARKAAKDVG ALYNEQLVSM DVHGGYVPQD AGGFIAINAV
410
RIREHVRAFG AYDVPTKKN
Length:419
Mass (Da):46,595
Last modified:May 1, 1999 - v1
Checksum:i0566A10A329BE513
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001572 Genomic DNA Translation: AAD19816.1
AE014297 Genomic DNA Translation: AAF54103.2
BT060440 mRNA Translation: ACN22205.1
RefSeqiNP_001262324.1, NM_001275395.1
NP_649674.1, NM_141417.3
UniGeneiDm.20149

Genome annotation databases

EnsemblMetazoaiFBtr0081699; FBpp0081197; FBgn0026565
FBtr0337022; FBpp0307951; FBgn0026565
GeneIDi40812
KEGGidme:Dmel_CG1315

Similar proteinsi

Entry informationi

Entry nameiASSY_DROME
AccessioniPrimary (citable) accession number: O97069
Secondary accession number(s): C0HDN4, Q9VI41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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