O97067 (PTH2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable peptidyl-tRNA hydrolase 2
Short name=PTH 2
EC=3.1.1.29

Gene names

ORF Names:

CG1307

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity.
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA.
Sequence similarities	Belongs to the PTH2 family.
Sequence caution	The sequence AAD19814.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM52710.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG15459	Q9VR92	1	EBI-197419,EBI-129983
CG9368	Q8MYU6	1	EBI-197419,EBI-173969
NC2alpha	Q9W2E3	1	EBI-197419,EBI-99385
RpLP2	P05389	1	EBI-197419,EBI-85954

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Probable peptidyl-tRNA hydrolase 2		PRO_0000120282

Sequences

	Sequence		Length	Mass (Da)
	O97067 [UniParc]. Last modified February 28, 2003. Version 2. Checksum: F431CCF19F4D8707	FASTA	186	19,873
	10 20 30 40 50 60 MGDKLLDPTQ IINGLAVMLS FFVGYRYALK RGDAKDSVTE GAATPFSQES SVSSGSEASV 70 80 90 100 110 120 SDKGYGGLND NFKMVLVVRN DLKMGKGKIA AQCGHGAVGA YQRAVVRTPR LLRSWENCGC 130 140 150 160 170 180 AKIAVRVESE AELMAIKKEA ERQQLNTCLI RDAGRTQIEA NSKTVLAVGP AAAADIDRVT GHLKLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of the Antennapedia complex of Drosophila." Celniker S.E., Pfeiffer B., Knafels J., Martin C.H., Mayeda C.A., Palazzolo M.J. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Berkeley.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001572 Genomic DNA. Translation: AAD19814.1. Sequence problems. AE014297 Genomic DNA. Translation: AAN13366.1. AY122198 mRNA. Translation: AAM52710.1. Different initiation.
RefSeq	NP_649675.2. NM_141418.3. NP_731106.1. NM_169155.1.
UniGene	Dm.11600.
3D structure databases
ProteinModelPortal	O97067.
SMR	O97067. Positions 70-186.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17218N.
IntAct	O97067. 7 interactions.
STRING	O97067.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0301623; FBpp0290838; FBgn0026566.
GeneID	40814.
KEGG	dme:Dmel_CG1307.
NMPDR	fig|7227.3.peg.11434.
Organism-specific databases
FlyBase	FBgn0026566. CG1307.
Phylogenomic databases
eggNOG	inNOG11006.
GeneTree	EMGT00050000005884.
InParanoid	O97067.
OMA	MEYLVHP.
OrthoDB	EOG4CJT0W.
PhylomeDB	O97067.
Gene expression databases
Bgee	O97067.
GermOnline	CG1307. Drosophila melanogaster.
Family and domain databases
InterPro	IPR023476. Pep_tRNA_hydro_II_dom. IPR002833. Pep_tRNA_hydro_PTH2. [Graphical view]
Gene3D	G3DSA:3.40.1490.10. G3DSA:3.40.1490.10. 1 hit.
KO	K04794.
Pfam	PF01981. PTH2. 1 hit. [Graphical view]
SUPFAM	SSF102462. SSF102462. 1 hit.
TIGRFAMs	TIGR00283. Arch_pth2. 1 hit.
ProtoNet	Search...
Other
NextBio	820724.

Entry information

Entry name

PTH2_DROME

Accession

Primary (citable) accession number: O97067
Secondary accession number(s): Q8MR09, Q9VI42

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 28, 2003
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project