O97048 (MA165_PINFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: N16.5 matrix protein Alternative name(s): N14#5 N14#7 Pearlin
Organism	Pinctada fucata (Pearl oyster)
Taxonomic identifier	50426 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Bivalvia › Pteriomorphia › Pterioida › Pterioidea › Pteriidae › Pinctada

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be specifically involved in the formation of the nacreous layer By similarity. Ref.3 Ref.4
Subunit structure	Heterooligomer; disulfide-linked. Pif97, Pif80, N16 and other proteins form a complex. Ref.3
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Component of conchiolin, the organic matrix of nacre. Specifically expressed in mantle epithelium.
Sequence similarities	Belongs to the N16 matrix protein family.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Repeat Signal
PTM	Disulfide bond
Gene Ontology (GO)
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 129	106	N16.5 matrix protein		PRO_0000379790
Regions
	Repeat	91 – 92	2	1
	Repeat	93 – 94	2	2
	Repeat	95 – 96	2	3
	Repeat	97 – 98	2	4
	Repeat	99 – 100	2	5
	Region	91 – 100	10	5 X 2 AA tandem repeats of N-G

Sequences

	Sequence		Length	Mass (Da)
	O97048 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6447712EA0940A9C	FASTA	129	15,388
	10 20 30 40 50 60 MTCTLRWTIT ALVLLGICHL ARPAFRTKCG RYSYCWIPYD IERDRYDNGD KKCCFCRNAW 70 80 90 100 110 120 SPWQCKEDER YEWLRCGHKF YYMCCYTDDD NGNGNGNGNG FNYLKSLYGG YGNGNGEFWE EYIDERYDK

References

[1]	"Complementary DNA cloning and characterization of pearlin, a new class of matrix protein in the nacreous layer of oyster pearls." Miyashita T., Takagi R., Okushima M., Nakano S., Miyamoto H., Nishikawa E., Matsushiro A. Mar. Biotechnol. 2:409-418(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mantle.
[2]	"14 kD matrix protein family in nacreous layer of Japanese peal oyster, Pinctada fucata." Hayashi N., Samata T. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mantle.
[3]	"An acidic matrix protein, Pif, is a key macromolecule for nacre formation." Suzuki M., Saruwatari K., Kogure T., Yamamoto Y., Nishimura T., Kato T., Nagasawa H. Science 325:1388-1390(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, FUNCTION.
[4]	"The molecular basis of nacre formation." Kroger N. Science 325:1351-1352(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Web resources

Protein Spotlight

String of intrusion - Issue 112 of December 2009

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB020779 mRNA. Translation: BAA75626.1. AB023252 mRNA. Translation: BAA83737.1. AB023253 mRNA. Translation: BAA83738.1. AB023254 mRNA. Translation: BAA83739.1.
3D structure databases
ProteinModelPortal	O97048.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MA165_PINFU

Accession

Primary (citable) accession number: O97048

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 1, 1999
Last modified:	October 3, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)