ID   FYV1_DROME              Reviewed;        1809 AA.
AC   O96838; A4UZM3; Q8SZX0; Q95SX9; Q9V886;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2004, sequence version 2.
DT   07-JUL-2009, entry version 64.
DE   RecName: Full=Putative FYVE finger-containing phosphoinositide kinase fab1;
DE            EC=2.7.1.68;
DE   AltName: Full=1-phosphatidylinositol-4-phosphate 5-kinase;
DE            Short=PtdIns(4)P-5-kinase;
DE            Short=PIP5K;
GN   Name=fab1; ORFNames=CG6355;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA   Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA   Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA   Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA   Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA   Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA   Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA   Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA   Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA   de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA   Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA   Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA   Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA   Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA   Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA   Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA   Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA   Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA   Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA   Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA   Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA   Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA   Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA   Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA   Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA   Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA   Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA   Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA   Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA   Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RX   MEDLINE=22426069; PubMed=12537572;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA   Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA   Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a
RT   systematic review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oregon-R;
RX   MEDLINE=20196011; PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA   Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D.,
RA   Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E.,
RA   Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D.,
RA   Minana B., Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S.,
RA   Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B.,
RA   Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F.,
RA   Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A.,
RA   Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA   McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA   Glover D.M.;
RT   "From sequence to chromosome: the tip of the X chromosome of D.
RT   melanogaster.";
RL   Science 287:2220-2222(2000).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 979-1809.
RC   STRAIN=Berkeley; TISSUE=Embryo, Larva, and Pupae;
RX   MEDLINE=22426066; PubMed=12537569;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA   George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA   Rubin G.M., Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=16837550; DOI=10.1091/mbc.E06-03-0239;
RA   Rusten T.E., Rodahl L.M.W., Pattni K., Englund C., Samakovlis C.,
RA   Dove S., Brech A., Stenmark H.;
RT   "Fab1 phosphatidylinositol 3-phosphate 5-kinase controls trafficking
RT   but not silencing of endocytosed receptors.";
RL   Mol. Biol. Cell 17:3989-4001(2006).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-755; THR-760 AND
RP   SER-1530, AND MASS SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18327897; DOI=10.1021/pr700696a;
RA   Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT   "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL   J. Proteome Res. 7:1675-1682(2008).
CC   -!- FUNCTION: Supports the intracellular PIP pool and to a lesser
CC       extent, the PI 4,5-P(2) pool. It generates PIP from PI and, to a
CC       lesser extent, PI 4,5-P(2) from PI 4-P. There are indications that
CC       it phosphorylates the D-5 rather than the D-4 position. Has a role
CC       at a late stage in endosome-related membrane trafficking, at a
CC       point when signal termination has occurred. Is not required for
CC       receptor silencing.
CC   -!- CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-
CC       phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
CC   -!- COFACTOR: Zinc (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endosome membrane. Note=Mainly associated
CC       with membranes of the late endocytic pathway.
CC   -!- DISRUPTION PHENOTYPE: Flies contain undetectable
CC       phosphatidylinositol 3,5-bisphosphate levels, show profound
CC       increases in cell and organ size, and die at the pupal stage.
CC       Mutant larvae contain highly enlarged multivesicular bodies and
CC       late endosomes that are inefficiently acidified. Even though
CC       endocytic receptor trafficking is impaired in fab1 mutants, Notch,
CC       Wingless, and Dpp signaling is unaffected.
CC   -!- SIMILARITY: Contains 1 DEP domain.
CC   -!- SIMILARITY: Contains 1 FYVE-type zinc finger.
CC   -!- SIMILARITY: Contains 1 PIPK domain.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA22949.1; Type=Erroneous gene model prediction;
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DR   EMBL; AE013599; AAF57789.1; -; Genomic_DNA.
DR   EMBL; AE013599; AAS64818.1; -; Genomic_DNA.
DR   EMBL; AL035311; CAA22949.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY060436; AAL25475.1; ALT_INIT; mRNA.
DR   EMBL; AY069736; AAL39881.1; ALT_INIT; mRNA.
DR   PIR; T13576; T13576.
DR   RefSeq; NP_611269.1; -.
DR   RefSeq; NP_995880.1; -.
DR   UniGene; Dm.7091; -.
DR   IntAct; O96838; 1.
DR   Ensembl; FBgn0028741; Drosophila melanogaster.
DR   GeneID; 37033; -.
DR   KEGG; dme:Dmel_CG6355; -.
DR   FlyBase; FBgn0028741; fab1.
DR   HOGENOM; O96838; -.
DR   OMA; O96838; IPYAQSY.
DR   BioCyc; DMEL-XXX-02:DMEL-XXX-02-005232-MON; -.
DR   BRENDA; 2.7.1.68; 48.
DR   NextBio; 801599; -.
DR   GermOnline; CG6355; Drosophila melanogaster.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0012506; C:vesicle membrane; ISS:UniProtKB.
DR   GO; GO:0000285; F:1-phosphatidylinositol-3-phosphate 5-kinase...; IMP:FlyBase.
DR   GO; GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase...; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005515; F:protein binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR   GO; GO:0044267; P:cellular protein metabolic process; IEA:InterPro.
DR   GO; GO:0008333; P:endosome to lysosome transport; IMP:FlyBase.
DR   GO; GO:0007242; P:intracellular signaling cascade; ISS:UniProtKB.
DR   GO; GO:0046488; P:phosphatidylinositol metabolic process; IEA:InterPro.
DR   GO; GO:0046854; P:phosphoinositide phosphorylation; IMP:FlyBase.
DR   InterPro; IPR002423; Cpn60/TCP-1.
DR   InterPro; IPR002498; PInositol-4-P-5-kinase_core.
DR   InterPro; IPR016034; PInositol-4P-5-kinase_core_sub.
DR   InterPro; IPR000591; Pleckstrin/G-protein_interact.
DR   InterPro; IPR000306; Znf_FYVE.
DR   InterPro; IPR017455; Znf_FYVE-rel.
DR   PANTHER; PTHR11353; Cpn60/TCP-1; 1.
DR   Pfam; PF00118; Cpn60_TCP1; 1.
DR   Pfam; PF01363; FYVE; 1.
DR   Pfam; PF01504; PIP5K; 1.
DR   SMART; SM00049; DEP; 1.
DR   SMART; SM00064; FYVE; 1.
DR   SMART; SM00330; PIPKc; 1.
DR   PROSITE; PS50186; DEP; 1.
DR   PROSITE; PS51455; PIPK; 1.
DR   PROSITE; PS50178; ZF_FYVE; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Endosome; Kinase; Membrane;
KW   Metal-binding; Nucleotide-binding; Phosphoprotein; Transferase; Zinc;
KW   Zinc-finger.
FT   CHAIN         1   1809       Putative FYVE finger-containing
FT                                phosphoinositide kinase fab1.
FT                                /FTId=PRO_0000185454.
FT   DOMAIN      323    399       DEP.
FT   DOMAIN     1473   1796       PIPK.
FT   ZN_FING     186    246       FYVE-type.
FT   REGION     1552   1809       Catalytic.
FT   MOD_RES     755    755       Phosphoserine.
FT   MOD_RES     760    760       Phosphothreonine.
FT   MOD_RES    1530   1530       Phosphoserine.
FT   CONFLICT   1403   1403       A -> ASSASA (in Ref. 3; CAA22949).
FT   CONFLICT   1420   1420       V -> I (in Ref. 3; CAA22949).
SQ   SEQUENCE   1809 AA;  204635 MW;  B5A111FAD317B05F CRC64;
     MTSNNQNNSS SHQHLHSPSK LTEFARNFED KPESLFGRVV NKIQNVYNQS YNTVNDISSG
     SSSSSSTQPV QVVGKSQFFS DSQTSTAEIA DVETSSQSSV RPQPPTTLSI RTNSETRGTS
     TSSNTAAEDS ETSDRVETLP LPTSEANQGR TVSNVLKHIS NIVATKNNND LRNYKDTELQ
     RFWMPDSKAK ECYDCSQKFS TFRRKHHCRL CGQIFCSKCC NQVVPGMIIR CDGDLKVCNY
     CSKIVLTFLK SSSSEMGQDM QELQQHLSNK LEVQDSGSSL AKHPQMQRAP LPRKTSVGYQ
     EERFSSHPTY TTLSIDDRKN ILQQSNSLIT LHEEMQRDLP AQNCGQRLIE FLNSNNKSAN
     EVQAVAILNA MLAAGFLEPI VPDPEQMDFD SSLHYKFSKS SSSDTSRTMS PQFEANPHAE
     PQPPKSMDQS AEEKEKELEN ELENDRCYTT ATSKLLASYC EHEEQLLAQM LRAHNLDQEW
     DKVLQMLCST AANHFKPEHC SNDLMDIRNY VNFKKVPGGR RKDSKIVHGV AFSKNVAHKD
     MATHVPFPRI LLLQCPIVYE RIEGKFVTIE TVLLQEKEYL RNVCARIMSF KPNVVLVHKN
     VAGIAQDLLR SYEVTLVLDV KLSVMERLSR TLQCDIVSSI ESNITMPKLG YCNDFYIRNY
     NGKTLMFFEK LTNPRGYTCL LRGGSNAELT RVKRVASALL FARYNWRLEM SFLLNEFAQP
     LSPKPSIFDS KETSPKTETE AELRSKRPII LERKSEDKIT TIVSENVSDF TDPLRASQAE
     ALSTSPCAPP VVEALAVEPR YDNRFRTALS STLLSVSPFL TFPLPYLETE QGRKCKLRKL
     FPAELYFSKQ WSRTGLERPD SMGDGEAGKS EPGNKENQMQ LLPAHDFVLM KITAPASSRD
     IQSKLAEFRS FGGRLPKGKA PMLRPKKKNA EVIQRPQKVS EEQLYKDALD PQNHQRLPVL
     FCSFHYNPKG VSSFCKLPML LDMKFYGQYD IMLEQFLQRY CCLFNSMCPS CNLPMLGHVR
     RYVHSLGCVH VYLTEDLTRS DPTRIYFTSW CSICNATTPT IPLSDAAKCL SLAKYLEMRF
     HGHAYKRRPP STDAEQGGTV CEHSLHRDYV HHFSFRGVGA KFQYTPVEVW ETDLPSLTVQ
     LDLPQPFQSA QVQEEIKNFS IKGHEVYNRI HERIADLATE EENSPLVQHL KTMLTHDQFI
     FKQKIEIVHT LLTDNRATAY DTSDALAMAR RALAESIELW GPRLQEIEKL TAKQAHHIDS
     GTICTEELRP EQVQTADSSK VTTSSLPKEN DPLECPSEDT ETGASNSQTV LDKNFSIDQM
     LASTVNVYSD KKSIRKILTQ LLPSGNQVNP LQSPFPAQDH LTLPLGSIPI HVRETDLSSV
     IAYSLTSMDY QKAIDEAEAN SNAAHSSPQL KRKIPLAESV SDAEDSPSLS RTSSNTSAAP
     NASVPSPATA ASESEEKSKE RIKQPPSPHI TLAFQDHSCQ FQCKIYFARE FDAMRSKSLK
     PPKLDKSLYR RLEKSKMREE LRISQSRTGS EMELVRKPSD VGAPRTTEDD SNQEEDARIA
     LARSLCKSVQ WEARGGKSGS RFCKTLDDRF VLKEMNSRDM TIFEPFAPKY FEYIDRCQQQ
     QQPTLLAKIF GVFRVSVKKK DSFVERSVMV MENLFYGCNI ENKFDLKGSE RNRLVDPSNQ
     QGEIVLLDEN LVQMSWSKPL YVLSHSKTVL RDAIQRDSSF LEKNLVMDYS LLVGLDKKNG
     VLVLGIIDYI RTFTLDKRVE SIIKGSGILG GKGKDPTVVN PERYKQRFID AMDRYFLTVP
     DRWEGLSKV
//