O96821 (CDC2H_PLAKH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division control protein 2 homolog
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:

CRK2

Organism

Plasmodium knowlesi (strain H)

Taxonomic identifier

5851 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Plasmodium) › Plasmodium knowlesi

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-158 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Cell division control protein 2 homolog		PRO_0000232670
Regions
	Domain	4 – 284	281	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	125	1	Proton acceptor By similarity
	Binding site	32	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	158	1	Phosphothreonine; by CAK By similarity

Sequences

	Sequence		Length	Mass (Da)
	O96821 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 02B0F83D5187AC68	FASTA	288	32,946
	10 20 30 40 50 60 MEKYHGLEKI GEGTYGVVYK AQNNYGETFA LKKIRLEKED EGIPSTAIRE ISILKELKHS 70 80 90 100 110 120 NIVKLYDVIH TKKRLILVFE HLDQDLKKLL DVCDGGLESV TAKSFLLQLL SGIAYCHEHR 130 140 150 160 170 180 VLHRDLKPQN LLINREGELK IADFGLARAF GIPVRKYTHE VVTLWYRAPD ILMGSKKYST 190 200 210 220 230 240 PIDIWSVGCI FAEMVNGRPL FPGVSETDQL MRIFRILGTP NSANWPSVTE LPKYDPDFIV 250 260 270 280 YEPLPWETFL KGLDDTGIDL LSKMLRLDPN QRITAKEALQ HAYFKESS

References

[1]

"Characterisation of the Cdc2-related kinase 2 gene from Plasmodium knowlesi and P. berghei." Vinkenoog R., Speranca M.A., Ramesar J., Thomas A.W., del Portillo H.A., Janse C.J., Waters A.P. Mol. Biochem. Parasitol. 95:229-240(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224155 Genomic DNA. Translation: CAA11852.1.
RefSeq	XP_002261323.1. XM_002261287.1.
3D structure databases
ProteinModelPortal	O96821.
SMR	O96821. Positions 1-286.
ModBase	Search...
Proteomic databases
PRIDE	O96821.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7321824.
KEGG	pkn:PKH_111410.
Organism-specific databases
EuPathDB	PlasmoDB:PKH_111410.
Phylogenomic databases
KO	K04563.
ProtClustDB	CLSZ2500781.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC2H_PLAKH

Accession

Primary (citable) accession number: O96821

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)