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Protein

Serine protease inhibitor dipetalogastin

Gene
N/A
Organism
Dipetalogaster maximus (Blood-sucking bug)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Thrombin inhibitor. Prevents blood clotting to allow insect to feed on blood. Also functions as an inhibitor of trypsin and plasmin.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei141 – 1422Reactive bondCurated

GO - Molecular functioni

  • serine-type endopeptidase inhibitor activity Source: UniProtKB

GO - Biological processi

  • negative regulation of coagulation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.022.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor dipetalogastin
Short name:
Dipetalin
OrganismiDipetalogaster maximus (Blood-sucking bug)Imported
Taxonomic identifieri72496 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraEuhemipteraHeteropteraPanheteropteraCimicomorphaReduviidaeTriatominaeDipetalogaster

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1311311 PublicationPRO_0000016580Add
BLAST
Chaini132 – 351220Serine protease inhibitor dipetalogastinPRO_0000016581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 50PROSITE-ProRule annotation
Disulfide bondi27 ↔ 46PROSITE-ProRule annotation
Disulfide bondi35 ↔ 67PROSITE-ProRule annotation
Disulfide bondi78 ↔ 103PROSITE-ProRule annotation
Disulfide bondi80 ↔ 99PROSITE-ProRule annotation
Disulfide bondi88 ↔ 120PROSITE-ProRule annotation
Disulfide bondi137 ↔ 162PROSITE-ProRule annotation
Disulfide bondi139 ↔ 158PROSITE-ProRule annotation
Disulfide bondi147 ↔ 179PROSITE-ProRule annotation
Disulfide bondi190 ↔ 215PROSITE-ProRule annotation
Disulfide bondi192 ↔ 211PROSITE-ProRule annotation
Disulfide bondi200 ↔ 232PROSITE-ProRule annotation
Disulfide bondi246 ↔ 271PROSITE-ProRule annotation
Disulfide bondi248 ↔ 267PROSITE-ProRule annotation
Disulfide bondi256 ↔ 287PROSITE-ProRule annotation
Disulfide bondi303 ↔ 328PROSITE-ProRule annotation
Disulfide bondi305 ↔ 324PROSITE-ProRule annotation
Disulfide bondi313 ↔ 345PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
351
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi134 – 1363Combined sources
Beta strandi153 – 1553Combined sources
Helixi157 – 16711Combined sources
Beta strandi175 – 1773Combined sources
Beta strandi179 – 1846Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KMANMR-A132-186[»]
ProteinModelPortaliO96790.
SMRiO96790. Positions 132-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO96790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 6951Kazal-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini72 – 12251Kazal-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini131 – 18151Kazal-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini184 – 23451Kazal-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini240 – 28950Kazal-like 5PROSITE-ProRule annotationAdd
BLAST
Domaini297 – 34751Kazal-like 6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 6 Kazal-like domains.PROSITE-ProRule annotationCurated

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 3 hits.
PF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 6 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 6 hits.
PS51465. KAZAL_2. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

O96790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
LIKELVNMVI QHAEEEEVKE LKNPCECPRA LHRVCGSDGN TYSNPCMLNC
60 70 80 90 100
AKHEGNPDLV QVHKGPCDEH DHDFEDPCKC DNKFEPVCGD DQITYLNLCH
110 120 130 140 150
LECATFTTSP GVEVAYEGEC HAETTNAMEV LFQGNPCECP RALHRVCGSD
160 170 180 190 200
GNTYSNPCML TCAKHEGNPD LVQVHEGPCD EHDHDFEDTC QCDDTFQPVC
210 220 230 240 250
GDDEITYRNL CHLECATFTT SPGVEVKHEG ECHPETKVNQ LILKSCMCPK
260 270 280 290 300
IYKPVCGTDG RTYPNICVLK CHISSNPGLG LAHLGECKVA VLAKETGEVR
310 320 330 340 350
NPCNCFRNFN PVCGTDGKTY GNLCMLGCAA ETKVPGLKLL HNGRCLPKEQ

L
Length:351
Mass (Da):38,690
Last modified:September 19, 2003 - v2
Checksum:i8B46FA8AE4319553
GO

Sequence cautioni

The sequence CAA10384.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131524 mRNA. Translation: CAA10384.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ131524 mRNA. Translation: CAA10384.1. Different initiation.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KMANMR-A132-186[»]
ProteinModelPortaliO96790.
SMRiO96790. Positions 132-186.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI01.022.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO96790.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 3 hits.
PF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 6 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 6 hits.
PS51465. KAZAL_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Dipetalogastin, a potent thrombin inhibitor from the blood-sucking insect Dipetalogaster maximus: cDNA cloning, expression, and characterization."
    Mende K., Petoukhova O., Koulitchkova V., Schaub G.A., Lange U., Kaufmann R., Nowak G.
    Eur. J. Biochem. 266:583-590(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Stomach.
  2. "Biochemical characterization of a thrombin inhibitor from the bloodsucking bug Dipetalogaster maximus."
    Lange U., Keilholz W., Schaub G.A., Landmann H., Markwardt F., Nowak G.
    Haemostasis 29:204-211(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 132-154, FUNCTION.
  3. "Interaction of Kazal-type inhibitor domains with serine proteinases: biochemical and structural studies."
    Schlott B., Woehnert J., Icke C., Hartmann M., Ramachandran R., Guehrs K.H., Glusa E., Flemming J., Goerlach M., Grosse F., Ohlenschlaeger O.
    J. Mol. Biol. 318:533-546(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 133-186.

Entry informationi

Entry nameiDPGN_DIPMA
AccessioniPrimary (citable) accession number: O96790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: July 6, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.