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O96771

- SYW_ENCCU

UniProt

O96771 - SYW_ENCCU

Protein

Tryptophan--tRNA ligase

Gene

ECU11_0530

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. tryptophan-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. tryptophanyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan--tRNA ligase (EC:6.1.1.2)
    Alternative name(s):
    Tryptophanyl-tRNA synthetase
    Short name:
    TrpRS
    Gene namesi
    Ordered Locus Names:ECU11_0530
    OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
    Taxonomic identifieri284813 [NCBI]
    Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
    ProteomesiUP000000819: Chromosome XI

    Organism-specific databases

    EuPathDBiMicrosporidiaDB:ECU11_0530.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385Tryptophan--tRNA ligasePRO_0000136742Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed in late sporogonial stages.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi6035.ECU11_0530.

    Structurei

    Secondary structure

    1
    385
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi25 – 306
    Turni31 – 333
    Helixi39 – 4911
    Helixi55 – 584
    Beta strandi61 – 677
    Helixi68 – 769
    Beta strandi81 – 877
    Helixi96 – 983
    Helixi99 – 11214
    Beta strandi116 – 1205
    Helixi122 – 1287
    Helixi133 – 14816
    Turni149 – 1513
    Helixi154 – 1563
    Beta strandi157 – 1615
    Helixi162 – 1654
    Helixi166 – 1694
    Helixi170 – 1789
    Helixi182 – 1898
    Helixi197 – 20711
    Helixi208 – 2103
    Helixi212 – 2143
    Beta strandi223 – 2297
    Helixi230 – 2323
    Helixi233 – 24311
    Helixi244 – 2463
    Beta strandi252 – 2565
    Helixi274 – 2763
    Helixi284 – 29411
    Helixi313 – 3153
    Helixi317 – 3259
    Helixi329 – 34012
    Helixi346 – 37025
    Helixi373 – 3808
    Helixi382 – 3843

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TZEX-ray2.60A/B1-385[»]
    ProteinModelPortaliO96771.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi89 – 9810"HIGH" region
    Motifi268 – 2725"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0180.
    HOGENOMiHOG000224742.
    KOiK01867.
    OMAiKTFIFNN.
    OrthoDBiEOG74N5SR.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    [Graphical view]
    PANTHERiPTHR10055. PTHR10055. 1 hit.
    PfamiPF00579. tRNA-synt_1b. 1 hit.
    [Graphical view]
    PRINTSiPR01039. TRNASYNTHTRP.
    TIGRFAMsiTIGR00233. trpS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O96771-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEQRITPWD VEVVSTDEVP VAIDYDKIIN QFGCEKFNQA LADRLEKLSG    50
    KPAHYFFRRG IVFAHRDFNL LLDEIANNRP FYLYTGRGPS SKTMHIGHTI 100
    PFLLCKYMQD AFKIRLVIQI TDDEKFLWKS MRLEDAMAYG RENIKDIVAL 150
    GFDPKLTYIF SNVEASHHFE ENILKISKTI NLNEAIKVFG FDMSSNIGQV 200
    GFPAKEIAPC FSSSFRFIGK GAMCLVPAAV DQDPFFRLAR DKAKALGEKK 250
    PSSIYVSLLP DLKGVNRKMS ASDPNSSIYL DDAQDTIRKK IIAYAYSGGR 300
    KTLEEHREKG GDIDVDVPFE YLKYFLDDDQ ELEKYRSGYI KGEITSKEMK 350
    EKCVVVIQEF VSRYQESRKR VTDDDLRAFI DINKF 385
    Length:385
    Mass (Da):44,189
    Last modified:August 31, 2004 - v2
    Checksum:i2865C3AC95FCC859
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590450 Genomic DNA. Translation: CAD25963.1.
    AJ012470 Genomic DNA. Translation: CAA10034.1.
    PIRiT43806.
    RefSeqiNP_586359.1. NM_001042192.1.

    Genome annotation databases

    GeneIDi860012.
    KEGGiecu:ECU11_0530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL590450 Genomic DNA. Translation: CAD25963.1 .
    AJ012470 Genomic DNA. Translation: CAA10034.1 .
    PIRi T43806.
    RefSeqi NP_586359.1. NM_001042192.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TZE X-ray 2.60 A/B 1-385 [» ]
    ProteinModelPortali O96771.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6035.ECU11_0530.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 860012.
    KEGGi ecu:ECU11_0530.

    Organism-specific databases

    EuPathDBi MicrosporidiaDB:ECU11_0530.

    Phylogenomic databases

    eggNOGi COG0180.
    HOGENOMi HOG000224742.
    KOi K01867.
    OMAi KTFIFNN.
    OrthoDBi EOG74N5SR.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    [Graphical view ]
    PANTHERi PTHR10055. PTHR10055. 1 hit.
    Pfami PF00579. tRNA-synt_1b. 1 hit.
    [Graphical view ]
    PRINTSi PR01039. TRNASYNTHTRP.
    TIGRFAMsi TIGR00233. trpS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GB-M1.
    2. "Microsporidia, amitochondrial protists, possess a 70-kDa heat shock protein gene of mitochondrial evolutionary origin."
      Peyretaillade E., Broussolle V., Peyret P., Metenier G., Gouy M., Vivares C.P.
      Mol. Biol. Evol. 15:683-689(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-134.
    3. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
      Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
      Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiSYW_ENCCU
    AccessioniPrimary (citable) accession number: O96771
    Secondary accession number(s): Q8SQY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3