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Protein

Tryptophan--tRNA ligase

Gene

ECU11_0530

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Ordered Locus Names:ECU11_0530
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU11_0530.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367421 – 385Tryptophan--tRNA ligaseAdd BLAST385

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi284813.NP_586359.1.

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 30Combined sources6
Turni31 – 33Combined sources3
Helixi39 – 49Combined sources11
Helixi55 – 58Combined sources4
Beta strandi61 – 67Combined sources7
Helixi68 – 76Combined sources9
Beta strandi81 – 87Combined sources7
Helixi96 – 98Combined sources3
Helixi99 – 112Combined sources14
Beta strandi116 – 120Combined sources5
Helixi122 – 128Combined sources7
Helixi133 – 148Combined sources16
Turni149 – 151Combined sources3
Helixi154 – 156Combined sources3
Beta strandi157 – 161Combined sources5
Helixi162 – 165Combined sources4
Helixi166 – 169Combined sources4
Helixi170 – 178Combined sources9
Helixi182 – 189Combined sources8
Helixi197 – 207Combined sources11
Helixi208 – 210Combined sources3
Helixi212 – 214Combined sources3
Beta strandi223 – 229Combined sources7
Helixi230 – 232Combined sources3
Helixi233 – 243Combined sources11
Helixi244 – 246Combined sources3
Beta strandi252 – 256Combined sources5
Helixi274 – 276Combined sources3
Helixi284 – 294Combined sources11
Helixi313 – 315Combined sources3
Helixi317 – 325Combined sources9
Helixi329 – 340Combined sources12
Helixi346 – 370Combined sources25
Helixi373 – 380Combined sources8
Helixi382 – 384Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TZEX-ray2.60A/B1-385[»]
ProteinModelPortaliO96771.
SMRiO96771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi89 – 98"HIGH" region10
Motifi268 – 272"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2145. Eukaryota.
COG0180. LUCA.
HOGENOMiHOG000224742.
InParanoidiO96771.
KOiK01867.
OMAiFFFSHRD.
OrthoDBiEOG092C2ZCA.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O96771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQRITPWD VEVVSTDEVP VAIDYDKIIN QFGCEKFNQA LADRLEKLSG
60 70 80 90 100
KPAHYFFRRG IVFAHRDFNL LLDEIANNRP FYLYTGRGPS SKTMHIGHTI
110 120 130 140 150
PFLLCKYMQD AFKIRLVIQI TDDEKFLWKS MRLEDAMAYG RENIKDIVAL
160 170 180 190 200
GFDPKLTYIF SNVEASHHFE ENILKISKTI NLNEAIKVFG FDMSSNIGQV
210 220 230 240 250
GFPAKEIAPC FSSSFRFIGK GAMCLVPAAV DQDPFFRLAR DKAKALGEKK
260 270 280 290 300
PSSIYVSLLP DLKGVNRKMS ASDPNSSIYL DDAQDTIRKK IIAYAYSGGR
310 320 330 340 350
KTLEEHREKG GDIDVDVPFE YLKYFLDDDQ ELEKYRSGYI KGEITSKEMK
360 370 380
EKCVVVIQEF VSRYQESRKR VTDDDLRAFI DINKF
Length:385
Mass (Da):44,189
Last modified:August 31, 2004 - v2
Checksum:i2865C3AC95FCC859
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590450 Genomic DNA. Translation: CAD25963.1.
AJ012470 Genomic DNA. Translation: CAA10034.1.
PIRiT43806.
RefSeqiNP_586359.1. NM_001042192.1.

Genome annotation databases

EnsemblFungiiCAD25963; CAD25963; CAD25963.
GeneIDi860012.
KEGGiecu:ECU11_0530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590450 Genomic DNA. Translation: CAD25963.1.
AJ012470 Genomic DNA. Translation: CAA10034.1.
PIRiT43806.
RefSeqiNP_586359.1. NM_001042192.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TZEX-ray2.60A/B1-385[»]
ProteinModelPortaliO96771.
SMRiO96771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284813.NP_586359.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAD25963; CAD25963; CAD25963.
GeneIDi860012.
KEGGiecu:ECU11_0530.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU11_0530.

Phylogenomic databases

eggNOGiKOG2145. Eukaryota.
COG0180. LUCA.
HOGENOMiHOG000224742.
InParanoidiO96771.
KOiK01867.
OMAiFFFSHRD.
OrthoDBiEOG092C2ZCA.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYW_ENCCU
AccessioniPrimary (citable) accession number: O96771
Secondary accession number(s): Q8SQY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 31, 2004
Last modified: November 30, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.