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O96771

- SYW_ENCCU

UniProt

O96771 - SYW_ENCCU

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Protein
Tryptophan--tRNA ligase
Gene
ECU11_0530
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. tryptophan-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Ordered Locus Names:ECU11_0530
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819: Chromosome XI

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU11_0530.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Tryptophan--tRNA ligase
PRO_0000136742Add
BLAST

Expressioni

Developmental stagei

Expressed in late sporogonial stages.

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi6035.ECU11_0530.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 306
Turni31 – 333
Helixi39 – 4911
Helixi55 – 584
Beta strandi61 – 677
Helixi68 – 769
Beta strandi81 – 877
Helixi96 – 983
Helixi99 – 11214
Beta strandi116 – 1205
Helixi122 – 1287
Helixi133 – 14816
Turni149 – 1513
Helixi154 – 1563
Beta strandi157 – 1615
Helixi162 – 1654
Helixi166 – 1694
Helixi170 – 1789
Helixi182 – 1898
Helixi197 – 20711
Helixi208 – 2103
Helixi212 – 2143
Beta strandi223 – 2297
Helixi230 – 2323
Helixi233 – 24311
Helixi244 – 2463
Beta strandi252 – 2565
Helixi274 – 2763
Helixi284 – 29411
Helixi313 – 3153
Helixi317 – 3259
Helixi329 – 34012
Helixi346 – 37025
Helixi373 – 3808
Helixi382 – 3843

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TZEX-ray2.60A/B1-385[»]
ProteinModelPortaliO96771.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi89 – 9810"HIGH" region
Motifi268 – 2725"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000224742.
KOiK01867.
OMAiKTFIFNN.
OrthoDBiEOG74N5SR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O96771-1 [UniParc]FASTAAdd to Basket

« Hide

MAEQRITPWD VEVVSTDEVP VAIDYDKIIN QFGCEKFNQA LADRLEKLSG    50
KPAHYFFRRG IVFAHRDFNL LLDEIANNRP FYLYTGRGPS SKTMHIGHTI 100
PFLLCKYMQD AFKIRLVIQI TDDEKFLWKS MRLEDAMAYG RENIKDIVAL 150
GFDPKLTYIF SNVEASHHFE ENILKISKTI NLNEAIKVFG FDMSSNIGQV 200
GFPAKEIAPC FSSSFRFIGK GAMCLVPAAV DQDPFFRLAR DKAKALGEKK 250
PSSIYVSLLP DLKGVNRKMS ASDPNSSIYL DDAQDTIRKK IIAYAYSGGR 300
KTLEEHREKG GDIDVDVPFE YLKYFLDDDQ ELEKYRSGYI KGEITSKEMK 350
EKCVVVIQEF VSRYQESRKR VTDDDLRAFI DINKF 385
Length:385
Mass (Da):44,189
Last modified:August 31, 2004 - v2
Checksum:i2865C3AC95FCC859
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590450 Genomic DNA. Translation: CAD25963.1.
AJ012470 Genomic DNA. Translation: CAA10034.1.
PIRiT43806.
RefSeqiNP_586359.1. NM_001042192.1.

Genome annotation databases

GeneIDi860012.
KEGGiecu:ECU11_0530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL590450 Genomic DNA. Translation: CAD25963.1 .
AJ012470 Genomic DNA. Translation: CAA10034.1 .
PIRi T43806.
RefSeqi NP_586359.1. NM_001042192.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TZE X-ray 2.60 A/B 1-385 [» ]
ProteinModelPortali O96771.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6035.ECU11_0530.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 860012.
KEGGi ecu:ECU11_0530.

Organism-specific databases

EuPathDBi MicrosporidiaDB:ECU11_0530.

Phylogenomic databases

eggNOGi COG0180.
HOGENOMi HOG000224742.
KOi K01867.
OMAi KTFIFNN.
OrthoDBi EOG74N5SR.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view ]
PANTHERi PTHR10055. PTHR10055. 1 hit.
Pfami PF00579. tRNA-synt_1b. 1 hit.
[Graphical view ]
PRINTSi PR01039. TRNASYNTHTRP.
TIGRFAMsi TIGR00233. trpS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.
  2. "Microsporidia, amitochondrial protists, possess a 70-kDa heat shock protein gene of mitochondrial evolutionary origin."
    Peyretaillade E., Broussolle V., Peyret P., Metenier G., Gouy M., Vivares C.P.
    Mol. Biol. Evol. 15:683-689(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-134.
  3. "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
    Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
    Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiSYW_ENCCU
AccessioniPrimary (citable) accession number: O96771
Secondary accession number(s): Q8SQY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 31, 2004
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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