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O96771 (SYW_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Ordered Locus Names:ECU11_0530
OrganismEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]
Taxonomic identifier284813 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Subunit structure

Homodimer By similarity.

Developmental stage

Expressed in late sporogonial stages.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385Tryptophan--tRNA ligase
PRO_0000136742

Regions

Motif89 – 9810"HIGH" region
Motif268 – 2725"KMSKS" region

Secondary structure

........................................................... 385
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O96771 [UniParc].

Last modified August 31, 2004. Version 2.
Checksum: 2865C3AC95FCC859

FASTA38544,189
        10         20         30         40         50         60 
MAEQRITPWD VEVVSTDEVP VAIDYDKIIN QFGCEKFNQA LADRLEKLSG KPAHYFFRRG 

        70         80         90        100        110        120 
IVFAHRDFNL LLDEIANNRP FYLYTGRGPS SKTMHIGHTI PFLLCKYMQD AFKIRLVIQI 

       130        140        150        160        170        180 
TDDEKFLWKS MRLEDAMAYG RENIKDIVAL GFDPKLTYIF SNVEASHHFE ENILKISKTI 

       190        200        210        220        230        240 
NLNEAIKVFG FDMSSNIGQV GFPAKEIAPC FSSSFRFIGK GAMCLVPAAV DQDPFFRLAR 

       250        260        270        280        290        300 
DKAKALGEKK PSSIYVSLLP DLKGVNRKMS ASDPNSSIYL DDAQDTIRKK IIAYAYSGGR 

       310        320        330        340        350        360 
KTLEEHREKG GDIDVDVPFE YLKYFLDDDQ ELEKYRSGYI KGEITSKEMK EKCVVVIQEF 

       370        380 
VSRYQESRKR VTDDDLRAFI DINKF 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi."
Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P.
Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GB-M1.
[2]"Microsporidia, amitochondrial protists, possess a 70-kDa heat shock protein gene of mitochondrial evolutionary origin."
Peyretaillade E., Broussolle V., Peyret P., Metenier G., Gouy M., Vivares C.P.
Mol. Biol. Evol. 15:683-689(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-134.
[3]"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages."
Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.
Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL590450 Genomic DNA. Translation: CAD25963.1.
AJ012470 Genomic DNA. Translation: CAA10034.1.
PIRT43806.
RefSeqNP_586359.1. NM_001042192.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3TZEX-ray2.60A/B1-385[»]
ProteinModelPortalO96771.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6035.ECU11_0530.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID860012.
KEGGecu:ECU11_0530.

Organism-specific databases

EuPathDBMicrosporidiaDB:ECU11_0530.

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000224742.
KOK01867.
OMAKTFIFNN.
OrthoDBEOG74N5SR.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_ENCCU
AccessionPrimary (citable) accession number: O96771
Secondary accession number(s): Q8SQY5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 31, 2004
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries