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Protein

Tryptophan--tRNA ligase

Gene

ECU11_0530

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Ordered Locus Names:ECU11_0530
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
Proteomesi
  • UP000000819 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU11_0530

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367421 – 385Tryptophan--tRNA ligaseAdd BLAST385

Expressioni

Developmental stagei

Expressed in late sporogonial stages.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi284813.NP_586359.1

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 30Combined sources6
Turni31 – 33Combined sources3
Helixi39 – 49Combined sources11
Helixi55 – 58Combined sources4
Beta strandi61 – 67Combined sources7
Helixi68 – 76Combined sources9
Beta strandi81 – 87Combined sources7
Helixi96 – 98Combined sources3
Helixi99 – 112Combined sources14
Beta strandi116 – 120Combined sources5
Helixi122 – 128Combined sources7
Helixi133 – 148Combined sources16
Turni149 – 151Combined sources3
Helixi154 – 156Combined sources3
Beta strandi157 – 161Combined sources5
Helixi162 – 165Combined sources4
Helixi166 – 169Combined sources4
Helixi170 – 178Combined sources9
Helixi182 – 189Combined sources8
Helixi197 – 207Combined sources11
Helixi208 – 210Combined sources3
Helixi212 – 214Combined sources3
Beta strandi223 – 229Combined sources7
Helixi230 – 232Combined sources3
Helixi233 – 243Combined sources11
Helixi244 – 246Combined sources3
Beta strandi252 – 256Combined sources5
Helixi274 – 276Combined sources3
Helixi284 – 294Combined sources11
Helixi313 – 315Combined sources3
Helixi317 – 325Combined sources9
Helixi329 – 340Combined sources12
Helixi346 – 370Combined sources25
Helixi373 – 380Combined sources8
Helixi382 – 384Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TZEX-ray2.60A/B1-385[»]
ProteinModelPortaliO96771
SMRiO96771
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi89 – 98"HIGH" region10
Motifi268 – 272"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2145 Eukaryota
COG0180 LUCA
HOGENOMiHOG000224742
InParanoidiO96771
KOiK01867
OMAiADILHPQ
OrthoDBiEOG092C2ZCA

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

O96771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQRITPWD VEVVSTDEVP VAIDYDKIIN QFGCEKFNQA LADRLEKLSG
60 70 80 90 100
KPAHYFFRRG IVFAHRDFNL LLDEIANNRP FYLYTGRGPS SKTMHIGHTI
110 120 130 140 150
PFLLCKYMQD AFKIRLVIQI TDDEKFLWKS MRLEDAMAYG RENIKDIVAL
160 170 180 190 200
GFDPKLTYIF SNVEASHHFE ENILKISKTI NLNEAIKVFG FDMSSNIGQV
210 220 230 240 250
GFPAKEIAPC FSSSFRFIGK GAMCLVPAAV DQDPFFRLAR DKAKALGEKK
260 270 280 290 300
PSSIYVSLLP DLKGVNRKMS ASDPNSSIYL DDAQDTIRKK IIAYAYSGGR
310 320 330 340 350
KTLEEHREKG GDIDVDVPFE YLKYFLDDDQ ELEKYRSGYI KGEITSKEMK
360 370 380
EKCVVVIQEF VSRYQESRKR VTDDDLRAFI DINKF
Length:385
Mass (Da):44,189
Last modified:August 31, 2004 - v2
Checksum:i2865C3AC95FCC859
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL590450 Genomic DNA Translation: CAD25963.1
AJ012470 Genomic DNA Translation: CAA10034.1
PIRiT43806
RefSeqiNP_586359.1, NM_001042192.1

Genome annotation databases

EnsemblFungiiCAD25963; CAD25963; CAD25963
GeneIDi860012
KEGGiecu:ECU11_0530

Similar proteinsi

Entry informationi

Entry nameiSYW_ENCCU
AccessioniPrimary (citable) accession number: O96771
Secondary accession number(s): Q8SQY5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 31, 2004
Last modified: March 28, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health