O96771 (SYW_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Ordered Locus Names:

ECU11_0530

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).
Subunit structure	Homodimer By similarity.
Developmental stage	Expressed in late sporogonial stages.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 385

385

Tryptophan--tRNA ligase

PRO_0000136742

Regions

Motif

89 – 98

10

"HIGH" region

Motif

268 – 272

5

"KMSKS" region

Secondary structure

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385

Helix Strand Turn

Details...

Helix

25 – 30

6

Turn

31 – 33

3

Helix

39 – 49

11

Helix

55 – 58

4

Beta strand

61 – 67

7

Helix

68 – 76

9

Beta strand

81 – 87

7

Helix

96 – 98

3

Helix

99 – 112

14

Beta strand

116 – 120

5

Helix

122 – 128

7

Helix

133 – 148

16

Turn

149 – 151

3

Helix

154 – 156

3

Beta strand

157 – 161

5

Helix

162 – 165

4

Helix

166 – 169

4

Helix

170 – 178

9

Helix

182 – 189

8

Helix

197 – 207

11

Helix

208 – 210

3

Helix

212 – 214

3

Beta strand

223 – 229

7

Helix

230 – 232

3

Helix

233 – 243

11

Helix

244 – 246

3

Beta strand

252 – 256

5

Helix

274 – 276

3

Helix

284 – 294

11

Helix

313 – 315

3

Helix

317 – 325

9

Helix

329 – 340

12

Helix

346 – 370

25

Helix

373 – 380

8

Helix

382 – 384

3

Sequences

	Sequence		Length	Mass (Da)
	O96771 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 2865C3AC95FCC859	FASTA	385	44,189
	10 20 30 40 50 60 MAEQRITPWD VEVVSTDEVP VAIDYDKIIN QFGCEKFNQA LADRLEKLSG KPAHYFFRRG 70 80 90 100 110 120 IVFAHRDFNL LLDEIANNRP FYLYTGRGPS SKTMHIGHTI PFLLCKYMQD AFKIRLVIQI 130 140 150 160 170 180 TDDEKFLWKS MRLEDAMAYG RENIKDIVAL GFDPKLTYIF SNVEASHHFE ENILKISKTI 190 200 210 220 230 240 NLNEAIKVFG FDMSSNIGQV GFPAKEIAPC FSSSFRFIGK GAMCLVPAAV DQDPFFRLAR 250 260 270 280 290 300 DKAKALGEKK PSSIYVSLLP DLKGVNRKMS ASDPNSSIYL DDAQDTIRKK IIAYAYSGGR 310 320 330 340 350 360 KTLEEHREKG GDIDVDVPFE YLKYFLDDDQ ELEKYRSGYI KGEITSKEMK EKCVVVIQEF 370 380 VSRYQESRKR VTDDDLRAFI DINKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[2]	"Microsporidia, amitochondrial protists, possess a 70-kDa heat shock protein gene of mitochondrial evolutionary origin." Peyretaillade E., Broussolle V., Peyret P., Metenier G., Gouy M., Vivares C.P. Mol. Biol. Evol. 15:683-689(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-134.
[3]	"Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages." Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C. Proteomics 6:3625-3635(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL590450 Genomic DNA. Translation: CAD25963.1.
AJ012470 Genomic DNA. Translation: CAA10034.1.

PIR

T43806.

RefSeq

NP_586359.1. NM_001042192.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TZE	X-ray	2.60	A/B	1-385	[»]

ProteinModelPortal

O96771.

ModBase

Search...

Protein-protein interaction databases

STRING

6035.ECU11_0530.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

860012.

KEGG

ecu:ECU11_0530.

Phylogenomic databases

eggNOG

COG0180.

HOGENOM

HOG000224742.

KO

K01867.

OMA

KTGGRET.

Family and domain databases

Gene3D

3.40.50.620. 1 hit.

InterPro

IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]

PANTHER

PTHR10055. PTHR10055. 1 hit.

Pfam

PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]

PRINTS

PR01039. TRNASYNTHTRP.

TIGRFAMs

TIGR00233. trpS. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SYW_ENCCU

Accession

Primary (citable) accession number: O96771
Secondary accession number(s): Q8SQY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 31, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program