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Protein

Alkyldihydroxyacetonephosphate synthase

Gene

eapA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.1 Publication

Catalytic activityi

1-acyl-glycerone 3-phosphate + a long-chain alcohol = an alkyl-glycerone 3-phosphate + a long-chain acid anion.1 Publication

Cofactori

FAD1 Publication

Pathwayi: ether lipid biosynthesis

This protein is involved in the pathway ether lipid biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway ether lipid biosynthesis and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei352 – 3521Important for enzyme activityBy similarity
Binding sitei447 – 4471Substrate
Active sitei508 – 5081Proton donor/acceptor1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1757FAD1 Publication
Nucleotide bindingi237 – 2437FAD1 Publication
Nucleotide bindingi250 – 2556FAD1 Publication
Nucleotide bindingi301 – 3077FAD1 Publication

GO - Molecular functioni

  • alcohol binding Source: dictyBase
  • alkylglycerone-phosphate synthase activity Source: dictyBase
  • FAD binding Source: UniProtKB
  • flavin adenine dinucleotide binding Source: dictyBase
  • oxidoreductase activity, acting on CH-OH group of donors Source: InterPro

GO - Biological processi

  • ether lipid biosynthetic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00781.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkyldihydroxyacetonephosphate synthase (EC:2.5.1.26)
Short name:
Alkyl-DHAP synthase
Alternative name(s):
Alkylglycerone-phosphate synthase
Gene namesi
Name:eapA
ORF Names:DDB_G0286183
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286183. agps.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi352 – 3521R → H: 30-fold reduction in activity. 1 Publication
Mutagenesisi447 – 4471R → L: Loss of activity. The FAD binds poorly to the mutant enzyme. 1 Publication
Mutagenesisi508 – 5081Y → F: Loss of activity. 1 Publication
Mutagenesisi544 – 5441H → I: Loss of activity. 1 Publication
Mutagenesisi545 – 5451H → I: Loss of activity. 1 Publication
Mutagenesisi546 – 5461H → I: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 611611Alkyldihydroxyacetonephosphate synthasePRO_0000128178Add
BLAST

Proteomic databases

PaxDbiO96759.
PRIDEiO96759.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-29370N.
STRINGi44689.DDB0191146.

Structurei

Secondary structure

1
611
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 184Combined sources
Beta strandi21 – 266Combined sources
Beta strandi30 – 334Combined sources
Turni35 – 373Combined sources
Beta strandi40 – 434Combined sources
Beta strandi45 – 473Combined sources
Helixi55 – 628Combined sources
Helixi83 – 853Combined sources
Helixi95 – 1028Combined sources
Helixi107 – 1093Combined sources
Helixi114 – 1196Combined sources
Helixi126 – 1338Combined sources
Beta strandi142 – 1454Combined sources
Helixi150 – 16314Combined sources
Beta strandi166 – 1727Combined sources
Beta strandi189 – 1935Combined sources
Helixi194 – 1963Combined sources
Beta strandi200 – 2045Combined sources
Turni205 – 2084Combined sources
Beta strandi209 – 2135Combined sources
Helixi218 – 22710Combined sources
Helixi239 – 2413Combined sources
Helixi244 – 2507Combined sources
Helixi257 – 2604Combined sources
Helixi263 – 2664Combined sources
Beta strandi267 – 2748Combined sources
Beta strandi277 – 2793Combined sources
Helixi294 – 2974Combined sources
Beta strandi306 – 3138Combined sources
Beta strandi319 – 33012Combined sources
Helixi331 – 34414Combined sources
Beta strandi349 – 3546Combined sources
Helixi356 – 3649Combined sources
Helixi376 – 38611Combined sources
Turni387 – 3893Combined sources
Turni392 – 3943Combined sources
Beta strandi396 – 4049Combined sources
Helixi406 – 42116Combined sources
Turni422 – 4243Combined sources
Beta strandi426 – 4327Combined sources
Helixi435 – 4384Combined sources
Helixi439 – 4424Combined sources
Helixi443 – 4519Combined sources
Turni452 – 4543Combined sources
Beta strandi455 – 46511Combined sources
Helixi466 – 48520Combined sources
Turni486 – 4883Combined sources
Beta strandi491 – 50111Combined sources
Beta strandi504 – 51411Combined sources
Helixi522 – 53716Combined sources
Helixi564 – 57512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UUUX-ray1.95A/B/C/D9-587[»]
2UUVX-ray1.99A/B/C/D9-587[»]
ProteinModelPortaliO96759.
SMRiO96759. Positions 9-586.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO96759.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini137 – 317181FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni544 – 5463Important for enzyme activityBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi609 – 6113Microbody targeting signalSequence analysis

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1233. Eukaryota.
COG0277. LUCA.
InParanoidiO96759.
KOiK00803.
OMAiEIMKWNG.
PhylomeDBiO96759.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR025650. Alkyl-DHAP_Synthase.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR11748:SF3. PTHR11748:SF3. 1 hit.
PfamiPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 2 hits.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O96759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGEKKEYPK EHIDLYQQIK WNGWGDTRKF LHQLKPSGTI AMTTPEVSSV
60 70 80 90 100
PLPSLRGFIK KELTLPGEED KPFVLDETPA LQIENIHVDP PKQYPEFVRE
110 120 130 140 150
LKAFFLPDQL KDDKLARITH TFGKSLRDLI RVRIGQVKNA PDLIVLPHSH
160 170 180 190 200
EEVERLVQLA HKYNVVIIPM GGGSNIVGAI EPVSNERFTV SIDMRRMNKV
210 220 230 240 250
LWVDRREMTA CIQVGIMGPE LEKQLHKQGV SLGHDPDSFE FSTLGGWLAT
260 270 280 290 300
CSSGHQSDKY GDIEDMAVSF RTVTPTGTLE LRNGARSGAG INYKHIILGS
310 320 330 340 350
EGTLGIITEA VMKVHAVPQA VEYYGFLFPT FAHAVSALQQ IRSSEVIPTM
360 370 380 390 400
IRVYDPEETQ LSFAWKPSKG AVSEFTSAMV KKYLHYIRSF DFKNVCLSII
410 420 430 440 450
GFEGPKKVVD FHRTSVFDIL SKNAAFGLGS APGKTWAEKR YDLPYIRDFL
460 470 480 490 500
LDHNMWVDVA ETTVSYANLQ TLWKDAKQTF VKHFKDQGIP AWICAHISHT
510 520 530 540 550
YTNGVCLYFI FASKQNENKD MAQYIEAKKL MTDIIFKYGG SLSHHHGVGY
560 570 580 590 600
EHVPWMTRYA TRGWINVYRS LKETIDPKDI CNPRKLIPTI KEENNKEPFL
610
FDVVNVKYPK L
Length:611
Mass (Da):69,408
Last modified:May 1, 1999 - v1
Checksum:i99B1221683350271
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010740 mRNA. Translation: CAA09333.1.
AAFI02000085 Genomic DNA. Translation: EAL64267.1.
PIRiJE0365.
RefSeqiXP_637836.1. XM_632744.1.

Genome annotation databases

EnsemblProtistsiDDB0191146; DDB0191146; DDB_G0286183.
GeneIDi8625550.
KEGGiddi:DDB_G0286183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010740 mRNA. Translation: CAA09333.1.
AAFI02000085 Genomic DNA. Translation: EAL64267.1.
PIRiJE0365.
RefSeqiXP_637836.1. XM_632744.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2UUUX-ray1.95A/B/C/D9-587[»]
2UUVX-ray1.99A/B/C/D9-587[»]
ProteinModelPortaliO96759.
SMRiO96759. Positions 9-586.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29370N.
STRINGi44689.DDB0191146.

Proteomic databases

PaxDbiO96759.
PRIDEiO96759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191146; DDB0191146; DDB_G0286183.
GeneIDi8625550.
KEGGiddi:DDB_G0286183.

Organism-specific databases

dictyBaseiDDB_G0286183. agps.

Phylogenomic databases

eggNOGiKOG1233. Eukaryota.
COG0277. LUCA.
InParanoidiO96759.
KOiK00803.
OMAiEIMKWNG.
PhylomeDBiO96759.

Enzyme and pathway databases

UniPathwayiUPA00781.

Miscellaneous databases

EvolutionaryTraceiO96759.
PROiO96759.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR025650. Alkyl-DHAP_Synthase.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR11748:SF3. PTHR11748:SF3. 1 hit.
PfamiPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 2 hits.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADAS_DICDI
AccessioniPrimary (citable) accession number: O96759
Secondary accession number(s): Q54LZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: December 9, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.