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Protein

Thymidine kinase

Gene

TK

Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071Proton acceptorSequence Analysis
Binding sitei139 – 1391Substrate; via amide nitrogenBy similarity
Metal bindingi164 – 1641ZincBy similarity
Metal bindingi167 – 1671ZincBy similarity
Metal bindingi203 – 2031ZincBy similarity
Metal bindingi206 – 2061ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 388ATPSequence Analysis
Nucleotide bindingi63 – 653ATPBy similarity
Nucleotide bindingi106 – 1094ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
Ordered Locus Names:ECU01_0740i
OrganismiEncephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Taxonomic identifieri284813 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaUnikaryonidaeEncephalitozoon
ProteomesiUP000000819: Chromosome I

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU01_0740i.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Thymidine kinasePRO_0000174951Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi6035.ECU01_0740i.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076391.
InParanoidiO96720.
KOiK00857.
OrthoDBiEOG7Z3FGM.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

O96720-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLAVLEWKS IKRRNAPTAE MTRGTLNFVT SPMNAGKTAN MLLRARHAAT
60 70 80 90 100
LGRRVLLAKP LSDTRHESSV IRSRCGIEMK CDLCAGPEFS FTKDVLYGDV
110 120 130 140 150
DILLVDEAQF LSSRQIDELR EVADVHGIPV WCYGLLTDFK KNLFEGSKRL
160 170 180 190 200
VELCDKMIEL DIVCYFCKAD GRFHLKYANG KAVVEGPSID ISIPGDGKFV
210 220 230
AVCHMCWTEK TSTSEEVQDP RVLCAKVIPV DR
Length:232
Mass (Da):25,958
Last modified:May 29, 2013 - v3
Checksum:i3017360E71AF9BBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391737 Genomic DNA. Translation: CAD24944.2.
AJ006824 Genomic DNA. Translation: CAA07261.1.
RefSeqiXP_965909.1. XM_960816.1.

Genome annotation databases

GeneIDi860249.
KEGGiecu:ECU01_0740i.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL391737 Genomic DNA. Translation: CAD24944.2.
AJ006824 Genomic DNA. Translation: CAA07261.1.
RefSeqiXP_965909.1. XM_960816.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6035.ECU01_0740i.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi860249.
KEGGiecu:ECU01_0740i.

Organism-specific databases

EuPathDBiMicrosporidiaDB:ECU01_0740i.

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076391.
InParanoidiO96720.
KOiK00857.
OrthoDBiEOG7Z3FGM.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)."
    Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P.
    Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GB-M1.
  3. "Identification of transcriptional signals in Encephalitozoon cuniculi widespread among Microsporidia phylum: support for accurate structural genome annotation."
    Peyretaillade E., Goncalves O., Terrat S., Dugat-Bony E., Wincker P., Cornman R.S., Evans J.D., Delbac F., Peyret P.
    BMC Genomics 10:607-607(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GB-M1.
  4. "Putative thymidine kinase of Encephalitozoon cuniculi (Microspora) from the chromosome I."
    Duffieux F., Peyret P., Roe B.A., Vivares C.P.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-232.

Entry informationi

Entry nameiKITH_ENCCU
AccessioniPrimary (citable) accession number: O96720
Secondary accession number(s): Q8SSL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 29, 2013
Last modified: January 7, 2015
This is version 71 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.