O96720 (KITH_ENCCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
Ordered Locus Names:	ECU01_0740i

Organism

Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) [Reference proteome]

Taxonomic identifier

284813 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Unikaryonidae › Encephalitozoon › Encephalitozoon cuniculi

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Thymidine kinase		PRO_0000174951
Regions
	Nucleotide binding	11 – 18	8	ATP Potential
	Nucleotide binding	43 – 45	3	ATP By similarity
	Nucleotide binding	86 – 89	4	ATP By similarity
Sites
	Active site	87	1	Proton acceptor Potential
	Metal binding	144	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	183	1	Zinc By similarity
	Metal binding	186	1	Zinc By similarity
	Binding site	119	1	Substrate; via amide nitrogen By similarity
Experimental info
	Sequence conflict	129	1	R → V in CAA07261. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O96720 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: B210B51FA2952D5D	FASTA	212	23,647
	10 20 30 40 50 60 MTRGTLNFVT SPMNAGKTAN MLLRARHAAT LGRRVLLAKP LSDTRHESSV IRSRCGIEMK 70 80 90 100 110 120 CDLCAGPEFS FTKDVLYGDV DILLVDEAQF LSSRQIDELR EVADVHGIPV WCYGLLTDFK 130 140 150 160 170 180 KNLFEGSKRL VELCDKMIEL DIVCYFCKAD GRFHLKYANG KAVVEGPSID ISIPGDGKFV 190 200 210 AVCHMCWTEK TSTSEEVQDP RVLCAKVIPV DR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Putative thymidine kinase of Encephalitozoon cuniculi (Microspora) from the chromosome I." Duffieux F., Peyret P., Roe B.A., Vivares C.P. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome I of the amitochondriate intracellular parasite Encephalitozoon cuniculi (Microspora)." Peyret P., Katinka M.D., Duprat S., Duffieux F., Barbe V., Barbazanges M., Weissenbach J., Saurin W., Vivares C.P. Genome Res. 11:198-207(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.
[3]	"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi." Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F., Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P., Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J., Vivares C.P. Nature 414:450-453(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GB-M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ006824 Genomic DNA. Translation: CAA07261.1. AL391737 Genomic DNA. Translation: CAD24944.1.
RefSeq	XP_965909.1. XM_960816.1.
3D structure databases
ProteinModelPortal	O96720.
ModBase	Search...
Protein-protein interaction databases
STRING	6035.ECU01_0740i.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	860249.
KEGG	ecu:ECU01_0740i.
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	HESSVIR.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_ENCCU

Accession

Primary (citable) accession number: O96720
Secondary accession number(s): Q8SSL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 31, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program