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O96693

- DXR_PLAFX

UniProt

O96693 - DXR_PLAFX

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Protein

1-deoxy-D-xylulose 5-phosphate reductoisomerase, apicoplast

Gene

DXR

Organism
Plasmodium falciparum (isolate HB3)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).1 Publication

Catalytic activityi

2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 1 divalent cation per subunit. Mg(2+) or Mn(2+).1 Publication

Enzyme regulationi

Inhibited by fosmidomycin and its derivative FT900098.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361NADP
Binding sitei205 – 2051Substrate
Binding sitei206 – 2061NADP
Metal bindingi231 – 2311Divalent metal cation
Binding sitei232 – 2321Substrate
Metal bindingi233 – 2331Divalent metal cation
Binding sitei293 – 2931Substrate
Metal bindingi315 – 3151Divalent metal cation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 896NADP
Nucleotide bindingi113 – 1175NADP

GO - Molecular functioni

  1. 1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. NADPH binding Source: InterPro

GO - Biological processi

  1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18397.
BRENDAi1.1.1.267. 1455.
UniPathwayiUPA00056; UER00092.

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase, apicoplast (EC:1.1.1.267)
Short name:
1-deoxyxylulose-5-phosphate reductoisomerase
Short name:
DOXP reductoisomerase
Short name:
DXP reductoisomerase
Gene namesi
Name:DXR
OrganismiPlasmodium falciparum (isolate HB3)
Taxonomic identifieri137071 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

Subcellular locationi

Plastidapicoplast 1 Publication

GO - Cellular componenti

  1. apicoplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apicoplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7272ApicoplastCuratedAdd
BLAST
Chaini73 – 4884161-deoxy-D-xylulose 5-phosphate reductoisomerase, apicoplastPRO_0000415608Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
488
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi78 – 847Combined sources
Helixi88 – 10316Combined sources
Beta strandi107 – 11610Combined sources
Helixi118 – 12811Combined sources
Beta strandi131 – 1366Combined sources
Helixi138 – 1403Combined sources
Helixi142 – 1454Combined sources
Helixi147 – 1504Combined sources
Beta strandi157 – 1604Combined sources
Helixi161 – 17010Combined sources
Beta strandi176 – 1794Combined sources
Helixi183 – 19513Combined sources
Beta strandi199 – 2024Combined sources
Helixi206 – 22116Combined sources
Beta strandi226 – 2294Combined sources
Helixi232 – 2409Combined sources
Helixi243 – 2464Combined sources
Beta strandi251 – 2533Combined sources
Helixi256 – 2594Combined sources
Beta strandi262 – 2698Combined sources
Turni273 – 2764Combined sources
Helixi279 – 2824Combined sources
Turni287 – 2893Combined sources
Helixi301 – 3077Combined sources
Helixi310 – 32314Combined sources
Helixi327 – 3293Combined sources
Beta strandi330 – 3345Combined sources
Beta strandi340 – 3467Combined sources
Beta strandi351 – 3555Combined sources
Helixi361 – 3699Combined sources
Helixi383 – 3864Combined sources
Beta strandi388 – 3903Combined sources
Turni396 – 3983Combined sources
Helixi400 – 41112Combined sources
Helixi415 – 43117Combined sources
Helixi437 – 45014Combined sources
Helixi460 – 48324Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AU8X-ray1.86A/B1-488[»]
3AU9X-ray1.90A/B1-488[»]
3AUAX-ray2.15A/B1-488[»]
4GAEX-ray2.30A/B75-488[»]
4KP7X-ray2.00A/B74-488[»]
ProteinModelPortaliO96693.
SMRiO96693. Positions 77-486.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO96693.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni269 – 2702Binding to substrate phosphate group
Regioni311 – 3155Substrate binding

Sequence similaritiesi

Belongs to the DXR family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00183. DXP_reductoisom.
InterProiIPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR30525. PTHR30525. 1 hit.
PfamiPF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view]
SUPFAMiSSF69055. SSF69055. 1 hit.
TIGRFAMsiTIGR00243. Dxr. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O96693-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKYIYIYFF FITITINDLV INNTSKCVSI ERRKNNAYIN YGIGYNGPDN
60 70 80 90 100
KITKSRRCKR IKLCKKDLID IGAIKKPINV AIFGSTGSIG TNALNIIREC
110 120 130 140 150
NKIENVFNVK ALYVNKSVNE LYEQAREFLP EYLCIHDKSV YEELKELVKN
160 170 180 190 200
IKDYKPIILC GDEGMKEICS SNSIDKIVIG IDSFQGLYST MYAIMNNKIV
210 220 230 240 250
ALANKESIVS AGFFLKKLLN IHKNAKIIPV DSEHSAIFQC LDNNKVLKTK
260 270 280 290 300
CLQDNFSKIN NINKIFLCSS GGPFQNLTMD ELKNVTSENA LKHPKWKMGK
310 320 330 340 350
KITIDSATMM NKGLEVIETH FLFDVDYNDI EVIVHKECII HSCVEFIDKS
360 370 380 390 400
VISQMYYPDM QIPILYSLTW PDRIKTNLKP LDLAQVSTLT FHKPSLEHFP
410 420 430 440 450
CIKLAYQAGI KGNFYPTVLN ASNEIANNLF LNNKIKYFDI SSIISQVLES
460 470 480
FNSQKVSENS EDLMKQILQI HSWAKDKATD IYNKHNSS
Length:488
Mass (Da):55,757
Last modified:May 1, 1999 - v1
Checksum:i4E280C81CDFAD3EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111813 Genomic DNA. Translation: AAD03739.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111813 Genomic DNA. Translation: AAD03739.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3AU8 X-ray 1.86 A/B 1-488 [» ]
3AU9 X-ray 1.90 A/B 1-488 [» ]
3AUA X-ray 2.15 A/B 1-488 [» ]
4GAE X-ray 2.30 A/B 75-488 [» ]
4KP7 X-ray 2.00 A/B 74-488 [» ]
ProteinModelPortali O96693.
SMRi O96693. Positions 77-486.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00056 ; UER00092 .
BioCyci MetaCyc:MONOMER-18397.
BRENDAi 1.1.1.267. 1455.

Miscellaneous databases

EvolutionaryTracei O96693.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
HAMAPi MF_00183. DXP_reductoisom.
InterProi IPR003821. DXP_reductoisomerase.
IPR013644. DXP_reductoisomerase_C.
IPR013512. DXP_reductoisomerase_N.
IPR026877. DXPR_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR30525. PTHR30525. 1 hit.
Pfami PF08436. DXP_redisom_C. 1 hit.
PF02670. DXP_reductoisom. 1 hit.
PF13288. DXPR_C. 1 hit.
[Graphical view ]
SUPFAMi SSF69055. SSF69055. 1 hit.
TIGRFAMsi TIGR00243. Dxr. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs."
    Jomaa H., Wiesner J., Sanderbrand S., Altincicek B., Weidemeyer C., Hintz M., Turbachova I., Eberl M., Zeidler J., Lichtenthaler H.K., Soldati D., Beck E.
    Science 285:1573-1576(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION.
    Strain: Isolate HB3.
  2. "Molecular basis of fosmidomycin's action on the human malaria parasite Plasmodium falciparum."
    Umeda T., Tanaka N., Kusakabe Y., Nakanishi M., Kitade Y., Nakamura K.T.
    Sci. Rep. 1:E9-E9(2011)
    Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) IN COMPLEXES WITH NADPH; FOSMIDOMYCIN DERIVATIVE FR900098; MAGNESIUM AND MANGANESE, COFACTOR, SUBUNIT.
    Strain: Isolate FCR-3.

Entry informationi

Entry nameiDXR_PLAFX
AccessioniPrimary (citable) accession number: O96693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: May 1, 1999
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3