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O96651 (TOP3B_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3-beta

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III beta
Gene names
Name:Top3beta
Synonyms:TOP3
ORF Names:CG3458
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length875 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA. Ref.1

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Developmental stage

Expressed during the first 6 hours of embryonic development, levels decline during larval and pupal stages to increase again during adulthood. Ref.1

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 875875DNA topoisomerase 3-beta
PRO_0000145196

Regions

Domain3 – 153151Toprim
Compositional bias819 – 85941Gly-rich

Sites

Active site3321O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Sequence conflict7471V → M in AAD13219. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O96651 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 3A26520C10AB6057

FASTA87596,973
        10         20         30         40         50         60 
MKSVLMVAEK PSLAASLAGI LSNGRCTAKR GTGNGCSTHE WTGNFRNEGS VHFRMTSVCG 

        70         80         90        100        110        120 
HVMSLDFNKK YNCWDKVDPI QLFGCATEKK ETNPKQNMRK FLAHEARGCD YLVLWLDCDK 

       130        140        150        160        170        180 
EGENICFEVM DAVKHVINNV YSDQVTYRAH FSAITEKDIK KAMETLGHPN ENEAKSVDAR 

       190        200        210        220        230        240 
QELDLRIGCA FTRFQTKFFQ DRYGDLDSSL ISYGPCQTPT LGFCVKRHDD IQTFKPESFW 

       250        260        270        280        290        300 
HLQLLAGQPE VTLEWARGRV FKKDIAIMLL NRVKEHKKAT VESVASKEAY KSKPQALNTV 

       310        320        330        340        350        360 
ELMRICSSGL GIGPFQAMQI AERLYTQGYI SYPRTETNQY PTNFDLPAVL HVLKPSADFG 

       370        380        390        400        410        420 
EEARSILGDI QTPRKGKDAG DHPPITPMKL GNRSDFDRDT WRVYEFICRH FMGTVSRDLK 

       430        440        450        460        470        480 
YRVTTAKLSV GMETFSCTAS VLIDAGFTKV MTWSAFGKDE PQPPFVQGTQ VAINDVRLIE 

       490        500        510        520        530        540 
SQTGPPDYLT ESELITLMEE HGIGTDASIP VHINNICQRN YVHIENGRKL MPTTLGIVLV 

       550        560        570        580        590        600 
HGYQKIDPEL VLPTMRTEVE RMLTLIAQGS ANFQDVLRHA IKIFKLKFMY FVKNIDSMDA 

       610        620        630        640        650        660 
LFEVSFSPLA ESGKAHSRCG KCRRYMKYIQ TKPARLHCSH CDETYALPIG NVKVYREFKC 

       670        680        690        700        710        720 
PLDDFDLLAF STGVKGRSYP FCPYCYNHPP FSDMPHLGGC NTCTNANCPH SLNTLGISSC 

       730        740        750        760        770        780 
VECPTGVLVL DCTLAPTWKL GCNRCDVIIN CFKGATKITV EEAKCQECGA QQVNVVYKSD 

       790        800        810        820        830        840 
KSKFKDGSEE KSGCIFCSAD FSHLVEKHRA VASRPVRSGG GFRGGKAGRG GGGMGGAAFG 

       850        860        870 
SGGAVTAGGG PNAGGGVRGS RVAKDKMGQL ASYFV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of Drosophila topoisomerase IIIbeta. Relaxation of hypernegatively supercoiled DNA."
Wilson T.M., Chen A.D., Hsieh T.-S.
J. Biol. Chem. 275:1533-1540(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[4]Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF099909 mRNA. Translation: AAD13219.1.
AE014298 Genomic DNA. Translation: AAF46144.1.
BT016116 mRNA. Translation: AAV37001.1.
RefSeqNP_511059.2. NM_078504.3.
UniGeneDm.2946.

3D structure databases

ProteinModelPortalO96651.
SMRO96651. Positions 1-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid58054. 2 interactions.
DIPDIP-22155N.
IntActO96651. 1 interaction.

Proteomic databases

PaxDbO96651.
PRIDEO96651.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0070930; FBpp0070891; FBgn0026015.
GeneID31565.
KEGGdme:Dmel_CG3458.

Organism-specific databases

CTD31565.
FlyBaseFBgn0026015. Top3beta.

Phylogenomic databases

eggNOGCOG0550.
GeneTreeENSGT00530000063430.
InParanoidO96651.
KOK03165.
OMAPPFVQGT.
OrthoDBEOG7RBZ7N.
PhylomeDBO96651.

Gene expression databases

BgeeO96651.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi31565.
NextBio774220.
PROO96651.

Entry information

Entry nameTOP3B_DROME
AccessionPrimary (citable) accession number: O96651
Secondary accession number(s): Q5U0X6, Q9W416
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase