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Protein

DNA topoisomerase 3-beta

Gene

Top3beta

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.By similarity1 Publication

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA topoisomerase type I activity Source: FlyBase

GO - Biological processi

  • DNA topological change Source: FlyBase
  • positive regulation of synapse assembly Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3-beta (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III beta
Gene namesi
Name:Top3beta
Synonyms:TOP3
ORF Names:CG3458
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0026015. Top3beta.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451961 – 875DNA topoisomerase 3-betaAdd BLAST875

Proteomic databases

PaxDbiO96651.
PRIDEiO96651.

Expressioni

Developmental stagei

Expressed during the first 6 hours of embryonic development, levels decline during larval and pupal stages to increase again during adulthood.1 Publication

Gene expression databases

BgeeiFBgn0026015.
ExpressionAtlasiO96651. baseline.
GenevisibleiO96651. DM.

Interactioni

Protein-protein interaction databases

BioGridi58054. 2 interactors.
DIPiDIP-22155N.
IntActiO96651. 1 interactor.
STRINGi7227.FBpp0070891.

Structurei

3D structure databases

ProteinModelPortaliO96651.
SMRiO96651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 153ToprimPROSITE-ProRule annotationAdd BLAST151

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi819 – 859Gly-richAdd BLAST41

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1956. Eukaryota.
COG0550. LUCA.
InParanoidiO96651.
KOiK03165.
OMAiICEYATT.
OrthoDBiEOG091G01EW.
PhylomeDBiO96651.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O96651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVLMVAEK PSLAASLAGI LSNGRCTAKR GTGNGCSTHE WTGNFRNEGS
60 70 80 90 100
VHFRMTSVCG HVMSLDFNKK YNCWDKVDPI QLFGCATEKK ETNPKQNMRK
110 120 130 140 150
FLAHEARGCD YLVLWLDCDK EGENICFEVM DAVKHVINNV YSDQVTYRAH
160 170 180 190 200
FSAITEKDIK KAMETLGHPN ENEAKSVDAR QELDLRIGCA FTRFQTKFFQ
210 220 230 240 250
DRYGDLDSSL ISYGPCQTPT LGFCVKRHDD IQTFKPESFW HLQLLAGQPE
260 270 280 290 300
VTLEWARGRV FKKDIAIMLL NRVKEHKKAT VESVASKEAY KSKPQALNTV
310 320 330 340 350
ELMRICSSGL GIGPFQAMQI AERLYTQGYI SYPRTETNQY PTNFDLPAVL
360 370 380 390 400
HVLKPSADFG EEARSILGDI QTPRKGKDAG DHPPITPMKL GNRSDFDRDT
410 420 430 440 450
WRVYEFICRH FMGTVSRDLK YRVTTAKLSV GMETFSCTAS VLIDAGFTKV
460 470 480 490 500
MTWSAFGKDE PQPPFVQGTQ VAINDVRLIE SQTGPPDYLT ESELITLMEE
510 520 530 540 550
HGIGTDASIP VHINNICQRN YVHIENGRKL MPTTLGIVLV HGYQKIDPEL
560 570 580 590 600
VLPTMRTEVE RMLTLIAQGS ANFQDVLRHA IKIFKLKFMY FVKNIDSMDA
610 620 630 640 650
LFEVSFSPLA ESGKAHSRCG KCRRYMKYIQ TKPARLHCSH CDETYALPIG
660 670 680 690 700
NVKVYREFKC PLDDFDLLAF STGVKGRSYP FCPYCYNHPP FSDMPHLGGC
710 720 730 740 750
NTCTNANCPH SLNTLGISSC VECPTGVLVL DCTLAPTWKL GCNRCDVIIN
760 770 780 790 800
CFKGATKITV EEAKCQECGA QQVNVVYKSD KSKFKDGSEE KSGCIFCSAD
810 820 830 840 850
FSHLVEKHRA VASRPVRSGG GFRGGKAGRG GGGMGGAAFG SGGAVTAGGG
860 870
PNAGGGVRGS RVAKDKMGQL ASYFV
Length:875
Mass (Da):96,973
Last modified:June 1, 2001 - v2
Checksum:i3A26520C10AB6057
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti747V → M in AAD13219 (PubMed:10636841).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099909 mRNA. Translation: AAD13219.1.
AE014298 Genomic DNA. Translation: AAF46144.1.
BT016116 mRNA. Translation: AAV37001.1.
RefSeqiNP_001284938.1. NM_001298009.1.
NP_511059.2. NM_078504.4.
UniGeneiDm.2946.

Genome annotation databases

EnsemblMetazoaiFBtr0070930; FBpp0070891; FBgn0026015.
FBtr0340028; FBpp0309042; FBgn0026015.
GeneIDi31565.
KEGGidme:Dmel_CG3458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099909 mRNA. Translation: AAD13219.1.
AE014298 Genomic DNA. Translation: AAF46144.1.
BT016116 mRNA. Translation: AAV37001.1.
RefSeqiNP_001284938.1. NM_001298009.1.
NP_511059.2. NM_078504.4.
UniGeneiDm.2946.

3D structure databases

ProteinModelPortaliO96651.
SMRiO96651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58054. 2 interactors.
DIPiDIP-22155N.
IntActiO96651. 1 interactor.
STRINGi7227.FBpp0070891.

Proteomic databases

PaxDbiO96651.
PRIDEiO96651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070930; FBpp0070891; FBgn0026015.
FBtr0340028; FBpp0309042; FBgn0026015.
GeneIDi31565.
KEGGidme:Dmel_CG3458.

Organism-specific databases

CTDi31565.
FlyBaseiFBgn0026015. Top3beta.

Phylogenomic databases

eggNOGiKOG1956. Eukaryota.
COG0550. LUCA.
InParanoidiO96651.
KOiK03165.
OMAiICEYATT.
OrthoDBiEOG091G01EW.
PhylomeDBiO96651.

Miscellaneous databases

GenomeRNAii31565.
PROiO96651.

Gene expression databases

BgeeiFBgn0026015.
ExpressionAtlasiO96651. baseline.
GenevisibleiO96651. DM.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP3B_DROME
AccessioniPrimary (citable) accession number: O96651
Secondary accession number(s): Q5U0X6, Q9W416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.