Reviewed, UniProtKB/Swiss-Prot O96559 (APY_CIMLE)

Names and origin

Protein names

Recommended name:
    Apyrase
    EC=3.6.1.5

Gene names

Name:

APY

Organism

Cimex lectularius (Bed bug)

Taxonomic identifier

79782 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Paraneoptera › Hemiptera › Euhemiptera › Heteroptera › Panheteroptera › Cimicomorpha › Cimicidae › Cimex

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits platlet aggregation by the calcium-dependent hydrolysis of ATP and ADP. Ref.1
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate. Ref.1
Cofactor	Calcium. Ref.1
Sequence similarities	Belongs to the apyrase family.

Ontologies

Keywords
Domain	Signal
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of blood coagulation Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular space Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-dependent ATPase activity Ref.1 Inferred from direct assay. Source: UniProtKB nucleoside-diphosphatase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Ref.1
	Chain	36 – 364	329	Apyrase Ref.1		PRO_0000234527
Regions
	Compositional bias	21 – 28	8	Poly-Leu

Sequences

	Sequence		Length	Mass (Da)
	O96559-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C398CB6D29A882DE	FASTA	364	41,474
	10 20 30 40 50 60 MRSSYRVGNP IRFQPTNVVG LLLLSLVLSF MLVQSYELGH ASGETNANSK YPLTTPVEEN 70 80 90 100 110 120 LKVRFKIGVI SDDDKNAVSK DESNTWVSTY LTGTLEWEKS TDKITVQWDK GNEKKVKSKY 130 140 150 160 170 180 SYGGRGMELS ELVTFNGNLL TFDDRTGLVY ILKDDKVYPW VVLADGDGKN SKGFKSEWAT 190 200 210 220 230 240 EKAGNLYVGS SGKEWTTKEG TIENYNPMWV KMINKNGEVT SLNWQTNYEK IRSSMNITFP 250 260 270 280 290 300 GYMWHEAACW SDKYNKWFFL PRALSQEAYD SKKFETQGAN VIISCDDKFE KCEPTQIQGK 310 320 330 340 350 360 TEDKRGFSNF KFVPTSEDKI IVGLKTVEAD DTTETYFTAF DLEGKVLLEE TKIDDHKYEG VDFV

References

[1]

"Purification, cloning, and expression of an apyrase from the bed bug Cimex lectularius. A new type of nucleotide-binding enzyme." Valenzuela J.G., Charlab R., Galperin M.Y., Ribeiro J.M.C. J. Biol. Chem. 273:30583-30590(1998) [PubMed: 9804829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 36-85; 263-284 AND 326-345, FUNCTION, CATALYTIC ACTIVITY, COFACTOR. Tissue: Salivary gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF085499 mRNA. Translation: AAD09177.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.5. 142462.
Family and domain databases
InterPro	IPR009283. Apyrase. [Graphical view]
PANTHER	PTHR13023. Apyrase. 1 hit.
Pfam	PF06079. Apyrase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APY_CIMLE

Accession

Primary (citable) accession number: O96559

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)