Reviewed, UniProtKB/Swiss-Prot O96539 (ATE1_DROME)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA--protein transferase 1
      Short name=Arginyltransferase 1
      Short name=R-transferase 1
    EC=2.3.2.8
Alternative name(s):
    Arginine-tRNA--protein transferase 1

Gene names

Name:	Ate1
ORF Names:	CG9204

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues By similarity.
Catalytic activity	L-arginyl-tRNA + protein = tRNA + L-arginyl-protein.
Sequence similarities	Belongs to the R-transferase family.
Sequence caution	The sequence AAO25000.1 differs from that shown. Reason: Frameshift at position 106. The sequence AAO25000.1 differs from that shown. Reason: Miscellaneous discrepancy. Deletion of many residues.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein arginylation Inferred from electronic annotation. Source: InterPro
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW arginyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: O96539-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A (identifier: O96539-2)

The sequence of this isoform differs from the canonical sequence as follows:
     175-181: Missing.

Isoform C (identifier: O96539-4)

The sequence of this isoform differs from the canonical sequence as follows:
     175-181: Missing.
     239-288: LRLIHVYDDE...KEHLQATPLQ → IVLVASSDTE...QRFLVKSPLK

Isoform D (identifier: O96539-5)

The sequence of this isoform differs from the canonical sequence as follows:
     239-288: LRLIHVYDDE...KEHLQATPLQ → IVLVASSDTE...QRFLVKSPLK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Arginyl-tRNA--protein transferase 1		PRO_0000195090
Natural variations
	Alternative sequence	175 – 181	7	Missing in isoform A and isoform C.		VSP_000339
	Alternative sequence	239 – 288	50	LRLIH…ATPLQ → IVLVASSDTERTCADAVIAL YRKYQITVHNDNPARLTLAS MQRFLVKSPLK in isoform C and isoform D.		VSP_011157

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified August 16, 2004. Version 3. Checksum: 38677BF50672AC6D	FASTA	484	55,317
	10 20 30 40 50 60 MSLSIVSYYG SQQSKCGYCA GANCSLSHGM HAYQLDCRDY QDLIDRGWRR CGYYCYKLRN 70 80 90 100 110 120 QETCCPCYTI KCNGLEFKLS KSNKRILRRI NRFLRDGKRE SKPEAGDGDG EADADYAIVA 130 140 150 160 170 180 PEVTASEPQP QLPDKSPPVI NVEQVASLAT AQRKPTKQAT AAAVEAPTLG SNKSSLLSSS 190 200 210 220 230 240 AAAPISNKPC KKAKQMRLDR RLAKLGDSAS YSTKSLTQEK TLRDFLNTDS ETNKHRLKLR 250 260 270 280 290 300 LIHVYDDEFR RTLPQSFALY KKYQISIHND PPKDQDAYKE HLQATPLQNE KPWDGPEMGY 310 320 330 340 350 360 GSFHQQYWLD DKLIAVGVID ILPGCVSSVY FFYDPDYSFL SLGTYGSLRE IELVQSLAEK 370 380 390 400 410 420 VPSLKYYYMG FYIHSCPKMR YKGKLSPSYL LCPETYEWLP LTDVIRAKLD EHKYQRLNED 430 440 450 460 470 480 PAARDVNEFL MEHLDEVKLL LGGRTRTDYK HFRQLRGTVS DDDIIIEYSK LVGKECARRM LYVK
	Isoform A. Checksum: B32B8200D1CC4CE8	FASTA	477	54,671
	10 20 30 40 50 60 MSLSIVSYYG SQQSKCGYCA GANCSLSHGM HAYQLDCRDY QDLIDRGWRR CGYYCYKLRN 70 80 90 100 110 120 QETCCPCYTI KCNGLEFKLS KSNKRILRRI NRFLRDGKRE SKPEAGDGDG EADADYAIVA 130 140 150 160 170 180 PEVTASEPQP QLPDKSPPVI NVEQVASLAT AQRKPTKQAT AAAVEAPTLG SNKSAAPISN 190 200 210 220 230 240 KPCKKAKQMR LDRRLAKLGD SASYSTKSLT QEKTLRDFLN TDSETNKHRL KLRLIHVYDD 250 260 270 280 290 300 EFRRTLPQSF ALYKKYQISI HNDPPKDQDA YKEHLQATPL QNEKPWDGPE MGYGSFHQQY 310 320 330 340 350 360 WLDDKLIAVG VIDILPGCVS SVYFFYDPDY SFLSLGTYGS LREIELVQSL AEKVPSLKYY 370 380 390 400 410 420 YMGFYIHSCP KMRYKGKLSP SYLLCPETYE WLPLTDVIRA KLDEHKYQRL NEDPAARDVN 430 440 450 460 470 EFLMEHLDEV KLLLGGRTRT DYKHFRQLRG TVSDDDIIIE YSKLVGKECA RRMLYVK
	Isoform C. Checksum: 0ED47DA90616B696	FASTA	478	54,348
	10 20 30 40 50 60 MSLSIVSYYG SQQSKCGYCA GANCSLSHGM HAYQLDCRDY QDLIDRGWRR CGYYCYKLRN 70 80 90 100 110 120 QETCCPCYTI KCNGLEFKLS KSNKRILRRI NRFLRDGKRE SKPEAGDGDG EADADYAIVA 130 140 150 160 170 180 PEVTASEPQP QLPDKSPPVI NVEQVASLAT AQRKPTKQAT AAAVEAPTLG SNKSAAPISN 190 200 210 220 230 240 KPCKKAKQMR LDRRLAKLGD SASYSTKSLT QEKTLRDFLN TDSETNKHRL KIVLVASSDT 250 260 270 280 290 300 ERTCADAVIA LYRKYQITVH NDNPARLTLA SMQRFLVKSP LKNEKPWDGP EMGYGSFHQQ 310 320 330 340 350 360 YWLDDKLIAV GVIDILPGCV SSVYFFYDPD YSFLSLGTYG SLREIELVQS LAEKVPSLKY 370 380 390 400 410 420 YYMGFYIHSC PKMRYKGKLS PSYLLCPETY EWLPLTDVIR AKLDEHKYQR LNEDPAARDV 430 440 450 460 470 NEFLMEHLDE VKLLLGGRTR TDYKHFRQLR GTVSDDDIII EYSKLVGKEC ARRMLYVK
	Isoform D. Checksum: D12F3150F3C10876	FASTA	485	54,994
	10 20 30 40 50 60 MSLSIVSYYG SQQSKCGYCA GANCSLSHGM HAYQLDCRDY QDLIDRGWRR CGYYCYKLRN 70 80 90 100 110 120 QETCCPCYTI KCNGLEFKLS KSNKRILRRI NRFLRDGKRE SKPEAGDGDG EADADYAIVA 130 140 150 160 170 180 PEVTASEPQP QLPDKSPPVI NVEQVASLAT AQRKPTKQAT AAAVEAPTLG SNKSSLLSSS 190 200 210 220 230 240 AAAPISNKPC KKAKQMRLDR RLAKLGDSAS YSTKSLTQEK TLRDFLNTDS ETNKHRLKIV 250 260 270 280 290 300 LVASSDTERT CADAVIALYR KYQITVHNDN PARLTLASMQ RFLVKSPLKN EKPWDGPEMG 310 320 330 340 350 360 YGSFHQQYWL DDKLIAVGVI DILPGCVSSV YFFYDPDYSF LSLGTYGSLR EIELVQSLAE 370 380 390 400 410 420 KVPSLKYYYM GFYIHSCPKM RYKGKLSPSY LLCPETYEWL PLTDVIRAKL DEHKYQRLNE 430 440 450 460 470 480 DPAARDVNEF LMEHLDEVKL LLGGRTRTDY KHFRQLRGTV SDDDIIIEYS KLVGKECARR MLYVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative splicing results in differential expression, activity, and localization of the two forms of arginyl-tRNA-protein transferase, a component of the N-end rule pathway." Kwon Y.T., Kashina A.S., Varshavsky A. Mol. Cell. Biol. 19:182-193(1999) [PubMed: 9858543] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B). Strain: Berkeley. Tissue: Embryo.
[5]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D). Strain: Berkeley. Tissue: Embryo.
[6]	"Independent emergence of alternative splicing of arginyl-tRNA-protein transferase in Drosophila melanogaster and mammals." Picaud F., Petit D., Maftah A. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 163-484 (ISOFORM C).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF079101 mRNA. Translation: AAD12369.1. AE013599 Genomic DNA. Translation: AAF57496.3. AE013599 Genomic DNA. Translation: AAF57497.3. AE013599 Genomic DNA. Translation: AAF57498.2. AY051688 mRNA. Translation: AAK93112.1. BT001498 mRNA. Translation: AAN71253.1. BT003243 mRNA. Translation: AAO25000.1. Sequence problems. AF354710 mRNA. Translation: AAL83965.1.
RefSeq	NP_477394.3. NP_725939.2. NP_725940.2.
UniGene	Dm.4513
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBtr0086318; FBpp0085627; FBgn0025720; Drosophila melanogaster. [Genome view]
GeneID	37288.
KEGG	dme:Dmel_CG9204.
UCSC	CG9204-RA. d. melanogaster.
Organism-specific databases
CTD	37288.
FlyBase	FBgn0025720. Ate1.
Phylogenomic databases
OMA	TERTCAD.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-005615-MON.
BRENDA	2.3.2.8. 48.
Gene expression databases
GermOnline	CG9204. Drosophila melanogaster.
Family and domain databases
InterPro	IPR017137. Arg-tRNA-P_Trfase_1_euk. IPR007472. Arg-tRNA-P_Trfase_C. IPR007471. Arg_tRNA_PTrfase_N. [Graphical view]
Pfam	PF04377. ATE_C. 1 hit. PF04376. ATE_N. 1 hit. [Graphical view]
PIRSF	PIRSF037207. ATE1_euk. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	802931.

Entry information

Entry name

ATE1_DROME

Accession

Primary (citable) accession number: O96539
Secondary accession number(s): Q7KRL0 , Q86PB1, Q8IH08, Q8N0R9, Q8T7L5, Q9V906, Q9V907

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project